• Asian Pacific Journal of Cancer Prevention
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• 제16권17호
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• pp.7935-7941
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• 2015

The aim of this study was to implement massive parallel sequencing (MPS) technology in clinical genetics testing. We developed and tested an amplicon-based method for resequencing the BRCA1 and BRCA2 genes on an Illumina MiSeq to identify disease-causing mutations in patients with hereditary breast or ovarian cancer (HBOC). The coding regions of BRCA1 and BRCA2 were resequenced in 96 HBOC patient DNA samples obtained from different sample types: peripheral blood leukocytes, whole blood drops dried on paper, and buccal wash epithelia. A total of 16 random DNA samples were characterized using standard Sanger sequencing and applied to optimize the variant calling process and evaluate the accuracy of the MPS-method. The best bioinformatics workflow included the filtration of variants using GATK with the following cut-offs: variant frequency >14%, coverage ($>25{\times}$) and presence in both the forward and reverse reads. The MPS method had 100% sensitivity and 94.4% specificity. Similar accuracy levels were achieved for DNA obtained from the different sample types. The workflow presented herein requires low amounts of DNA samples (170 ng) and is cost-effective due to the elimination of DNA and PCR product normalization steps.

• 응용통계연구
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• 제26권3호
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• pp.453-470
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• 2013

본 논문에서는 카드뮴의 반응-용량 모형에 대한 베이지안 분석을 실시하고 기준용량에 대한 추정값들을 유도하고 비교한다. 이를 위하여 독성물질에 대한 용량반응곡선에서 많이 활용되는 두 가지 모형을 사용하고, 카드뮴의 독성연구에 관련한 기존의 문헌으로 수집된 자료에 대한 성별, 연령, 인종, study code 등과 같은 소집단 간의 개별적 형질을 반영할 수 있는 베이지안 메타분석 관점에서의 모형분석을 실시한다. 이러한 두 가지 모형에 대한 베이지안 분석을 위하여 WinBUGS를 이용한 마르코프 연쇄 몬테칼로(Markov chain Monte Carlo; MCMC) 방법을 통하여 모수를 추정하고 이에 따른 다양한 기준용량들을 계산하고 비교해보았다. 베이지안 모형 적합뿐만 아니라 편차정보기준을 통해서 주어진 자료를 더 잘 설명하는 모형을 선택하는 베이지안 모형 선택을 고려하였고, 이를 실제 자료에 적용해본다.

• 한국자원식물학회지
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• 제14권3호
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• pp.241-246
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• 2001

옻나무 근삽에 의한 묘목의 생산성을 향상시키기 위하여 7종류의 삽상배지를 공시하여 신초발생율과 묘소질을 조사하였다 삽상배지는 PV(peatmoss vermiculite = 1 : 1)와 PVS(peat moss : vermiculite : sand = 1 : 1 : 2) 처리구에서 3주 후부터 신초가 발생되었고, 삽 식 10주 후의 신초발생율은 PVSC(peatmoss : vermiculite : sand : clay loam = 1 : 1 : 2 : 2)배지에서 80%로써 가장 높았다. 삽수당 신초형성수는 PH와 PVC 삽상배지가 2.2개로써 가장 많았고 HSC 삽상배지 는 1.3∼1.5개로써 가장 적었으나 통계적인 유의차는 인정되지 않았다. Peatmoss, vermiculite, sand 및 clay loam을 각각 1:1:2:2 (v/v/v/v)로 혼합한 PVSC 삽상배지가 옻나무 근삽의 삽상배지로 이용하기에 양호하였으며, 수장, 분지수, 엽수, 경직경 및 엽면적 등의 묘소질 역시 양호하여 본포에 이식하기에 적당하였다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.185-191
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• 2013

The purpose of this study is to improve production efficiency of vitrified-thawed transgenic bovine embryos. Transgenic bovine embryos were produced by injection of FIV-GFP lentiviral vector into perivitelline space of in vitro matured MII stage oocytes, and then in vitro fertilization. EGFP-expressing transgenic bovine blastocysts were cultured in serum-containing and serum-free medium. These blsatocysts were vitrified by pull and cut (PNC) container made with 0.25 cm plastic straw. Results indicate that total developmental rates of normal IVF embryo cultured in serum-containing and-free medium into blastocyst were not significantly different (22.3 vs 21.5%) and those of GFP-expressing transgenic bovine embryo into blastocyst showed no significant difference between serum-containing (13.9%) and-free medium (13.1%). However, developmental rate of GFP transgenic embryo was significantly (P<0.05) lower than its of normal IVF embryos. In additional study, we vitrified GFP transgenic normal bovine blastocysts using PNC vitrification method. Survival rate of vitrified-thawed GFP transgenic blastocyst (23.1%) was significantly (P<0.05) lower than its of normal blastocysts (68.9%). Although, survival rate of vitrified-thawed GFP transgenic blastocyst was lower than its of normal blastocyst, our result may suggested that PNC vitrification method is feasible to cryopreserve transgenic embryos. Our next plan will be the production of GFP express transgenic bovine derived from vitrified-thawed embryos using PNC method.

• 대한족부족관절학회지
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• 제2권2호
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• pp.88-92
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• 1998

The course of the sural nerve in the calf has been well documented, but there is a general lack of information concerning the distal course of the nerve. The purpose of this study was to describe the distal course of the sural nerve and its surgical implications. Seven fresh amputated specimens were dissected to show the anatomy of the sural nerve in the foot and ankle. At the level of about 10cm proximal to the plantar surface, the sural nerve coursed anteriorly and inferiorly away from the Achilles tendon. 2 to 4 lateral calcaneal branches arose. The first branch of the lateral calcaneal branches coursed along the lateral border of the Achilles tendon, and it arose at 8cm proximal to the plantar surface in 2 specimens, 12cm proximal to the plantar surface in 4 specimens, and at 12cm proximal to the plantar surface in one specimen. The main nerve trunk continued distally plantar to the peroneal tendons and divided into two terminal branches and crossed peroneus longus tendon at the level of the inferior border of the calcaneo-cuboid joint, at about 3cm(range, $2.5\sim3.0$)cm from the plantar surface. In conclusion, a longitudinal incision lateral to the Achilles tendon would cross the path of the sural nerve at about 10cm proximal to the plantar surface. When the first branch of them arise more than 10cm above the plantar surface, a logitudinal incision lateral to the Achilles tendon may be made without damage. The other lateral calcaneal branches will be cut when we make transverse incision paralled to the plantar surface. The terminal branch also may be in danger by the same transverse incision.

• 한국균학회지
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• 제24권1호통권76호
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• pp.67-80
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• 1996

Ganoderma속균은 약효가 인정되어 많이 인공 재배되어 이용되고 있으나 본 속균에 대한 기초적인 연구가 미흡한 실정이므로 본 연구에서는 영지 속균을 채집하여 분리 동정하였으며, 또한 배양적 특성과 형태적인 특징을 조사하였다. 영지속균은 경기도와 강원도 11개 장소의 참나무, 아카시나무, 오리나무 등에서와 6재배농가에서 채집되었다. 대부분의 영지는 하나의 버섯이 그루터기에서 형성되나 오리나무에서는 다수가 형성되었다. 영지속균의 균사 생장적온은 G. applanatum, G.lucidum, G. neo-japonicum에서는 $28{\sim}30^{\circ}C$이었고, G. tsugae에서는 $26^{\circ}C$이었으나 $32^{\circ}C$에서는 천천히 생장하였다. Hamda배지를 pH 6.2로 조정하였을 때 균사생장이 가창 좋았다. 6종의 공시균주에 대한 균사의 형태적인 특정으로 G. applanatum, G. lucidum, G. neo-japonicum은 staghorn를 형성하였으나 G.oregonens, G. ualeosiacum은 포도형의 가지를 가진 staghorn균사를 형성하였다. Clamp connection은 G. applanatum, G. lucidum, G. oregonense, G. valeosiacum균의 균사에서 관찰되었으나 node형을 가진 G. neo-japonioum에서는 형성되지 않았다. 후막포자는 G. applanatum, G. neo-japonicum에서 형성되었으며, cuticular cell는 G. lucidum, G. neo-japonicum, G. oregonense, G. tsugae의 균사에서 형성되었다.

• 대한후두음성언어의학회지
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• 제30권2호
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• pp.132-135
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• 2019

Spasmodic dysphonia is a focal laryngeal dystonia that results in involuntary spasms during speech. The etiology of spasmodic dysphonia is not yet defined, but it is presumed to be a neurological abnormality of central nervous system motor function. The treatment of choice for spasmodic dysphonia is botulinum toxin injection directly at the laryngeal muscles. However botulinum toxin injection requires repeated procedures. Many different kinds of surgical treatments have been introduced but the recurrence rate is still high. So we performed myomectomy with LASER and neurectomy with specially designed electrical surgical knife which can cut recurrent laryngeal nerve branch selectively with its noble curved section. We report a case of a 43-year-old male patient with spasmodic dysphonia treated by thyroarytenoid myoneurectomy.

• International Journal of Concrete Structures and Materials
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• 제6권4호
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• pp.247-250
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• 2012

Composites are somewhat more difficult to model than an isotropic material such as iron or steel due to the fact that each layer may have different orthotropic material properties. In finite element literature the asphalt mixes are represented by using rectangular meshes, not the actual picture of their cross-sections. Asphalt aggregate size and distribution in the asphalt concrete sample, aggregate shape, and fractured surface effects are ignored. In this research, the actual image of the sample including all these effects were directly considered in the finite element. The samples, were cut into cross-sections and were scanned. The image-processing toolbox of Labview was utilized in obtaining the rectangular gray images of the scanned images. In the rectangular sample the aggregates were white and the asphalt binders were black. The grayscale images were converted by LABVIEW into the format required by ANSYS as an input file, with the same dimensions. The nodes at the bottom of the model were constrained in both x and y directions. Left and right edges were symmetry and top was free. Certain amount of pressure was applied along the top surface to simulate the tire pressure.

• 한국잠사곤충학회지
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• 제17권1호
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• pp.27-29
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• 1975

춘잠기에 있어서의 가지뽕의 간벌수확의 효과와 합리적인 간벌방법을 구명하기 위하여 시험한 바 다음과 같은 결과를 얻었다. 1. 춘잠기에 있어서의 가지뽕의 간벌수확은 1주일 정도의 간격으로서는 가는 가지 간벌수확구를 제외하고는 별로 효과가 없다. 2. 가는 가지 간벌구는 표준구와의 사이에 유의차는 없지만 약간 증수가 되는 경향에 있고 특히 소량의 가지뽕이 필요한 4령기에 잔조를 벌채하게 되어 그 수확비율이 현실에 적합하다는 이점이 있다. 3. 굵은 가지를 간벌하는 것은 다른 어떠한 수확법보다 못하다. 수량이 많은 굵은가지를 간벌한 후 1주일 정도로서는 나머지 가는 가지의 발육이 크게 호전하지 못하는 까닭이라고 생각된다.

• 한국경영과학회지
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• 제24권3호
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• pp.93-107
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• 1999

We consider the development of an integer programming model and algorithm for the ATM switching node location problem. There are two kinds of facilities, hub facilities and remote facilities, with different capacities and installation costs. Each customer needs to be connected to one or more hub facilities via remote facilities, where the hub(remote) facilities need to be installed at the same candidate installation site of hub(remote) facility. We are given a set of customers with each demand requirements, a set of candidate installation sites of facilities, and connection costs between facilities, We need to determine the locations to place facilities, the number of facilities for each selected location, the set of customers who are connected to each installed hub facilities via installed remote facilities with minimum costs, while satisfying demand requirements of each customer. We formulate this problem as a general integer programming problem and solve it to optimality. In this paper, we develop a branch-and-cut algorithm with path variables. In the algorithm, we consider the integer knapsack polytope and derive valid inequalities. Computational experiments show that the algorithm works well in the real world situation. The results of this research can be used to develop optimization algorithms to solve capacitated facility location problems.