• Title/Summary/Keyword: curdling

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Effects of High-Pressure, Microbial Transglutaminase and Glucono-δ-Lactone on the Aggregation Properties of Skim Milk

  • Lee, Sang Yoon;Choi, Mi-Jung;Cho, Hyung-Yong;Davaatseren, Munkhtugs
    • Food Science of Animal Resources
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    • v.36 no.3
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    • pp.335-342
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    • 2016
  • The object in this study is to investigate the effects of high pressure and freezing processes on the curdling of skim milk depending on the presence of transglutaminase (TGase) and glucono-δ-lactone (GdL). Skim milk was treated with atmospheric freezing (AF), high pressure (HP), pressure-shift freezing (PSF) and high pressure sub-zero temperature (HPST) processing conditions. After freezing and pressure processing, these processed milk samples were treated with curdling agents, such as TGase and GdL. Pressurized samples (HP, PSF and HPST) had lower lightness than that of the control. In particular, PSF had the lowest lightness (p<0.05). Likewise, the PSF proteins were the most insoluble regardless of whether they were activated by TGase and GdL, indicating the highest rate of protein aggregation (p<0.05). Furthermore, the TGase/GdL reaction resulted in thick bands corresponding to masses larger than 69 kDa, indicating curdling. Casein bands were the weakest in PSF-treated milk, revealing that casein was prone to protein aggregation. PSF also had the highest G' value among all treatments after activation by TGase, implying that PSF formed the hardest curd. However, adding GdL decreased the G' values of the samples except HPST-treated samples. Synthetically, the PSF process was advantageous for curdling of skim milk.

Evaluation of Transglutaminase from Pig Plasma on the Quality of Milk Curd

  • Tseng, Tsai-Fuh;Liu, Deng-Cheng;Chen, Ming-Tsao
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.1
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    • pp.106-110
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    • 2002
  • The purpose of this study was to examine the effect of different levels of crude pig plasma transglutaminase (TGase) on the quality of milk curd. Different levels (0, 0.1, 0.2, 0.4, 0.8 and 1.0%) of crude pig plasma TGase was added to fresh milk, individually, and then incubated at $35^{\circ}C$. The time of milk curdling was recorded. Simultaneously, rheological properties, L value and microstructure of the samples were measured and observed. The results showed that the time of milk curdling decreased and the curd strength of milk curd increased with increasing of TGase (p<0.05). The softness of milk curd had the highest value when 0.8% TGase was added (p<0.05). However, L value of milk curds was not significantly different among all treatments. The microstructure of milk curd observed by scanning electron microscopy (SEM) became more dense with TGase level increased.

Exploration of optimal Lactobacillus plantarum strains for curdling milk for yogurt and evaluation of physicochemical and sensory properties (호상 요구르트에 적합한 Lactobacillus plantarum strains 탐색 및 요구르트의 이화학적 및 관능 특성)

  • Jeong, Seong-Yeop;Lee, Yong Hyen;Kang, Suna;Shin, Bae Keun;Park, Sunmin
    • Korean Journal of Food Science and Technology
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    • v.48 no.6
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    • pp.548-554
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    • 2016
  • Since some strains of kimchi lactobacilli can curdle milk, they can be used for making yogurt. However, the best Lactobacillus plantarum strains for curdling milk for yogurt are still unknown. In this study, we determined the best L. plantarum strains for curdling milk, and the physicochemical properties of yogurts made using different L. plantarum strains were examined. Three strains of L. plantarum useful for curdling milk were identified (YD2, YD9, YD12). The number of lactobacilli was lower in yogurts made with L. plantarum than in those made with control, and among the L. plantarum strains tested, YD12 had the highest bacterial counts. However, the microbial count reached $6.3{\times}10^8CFU/mL$ after 24-h fermentation in all yogurts. The pH of the yogurts decreased after 12-h fermentation, while the acidity increased. The low pH and high acidity decreased the viscosity in all the three types of yogurts, because the acids disturbed gel formation due to protein denaturation. Sensory evaluation revealed that the YD12 group showed a high percentage of completion similar to the control group. YD2 and YD9 showed a high sourness value and low sweetness value, whereas YD12 yielded optimal values for all the organoleptic characteristics. Therefore, YD12 would be a high quality bacterial strain for use as a yogurt starter culture.

A Study on the Variation of Physical Properties by the Water to Cement Ratio and the Mixing Speed for Grout Materials (그라우트재의 물시멘트비 및 혼합속도에 의한 물성변화에 관한 연구)

  • 천병식;김진춘;장의웅;송성호;이준우
    • Proceedings of the Korean Geotechical Society Conference
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    • 2001.03a
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    • pp.445-452
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    • 2001
  • Generally, OPC(ordinary portland cement) is used for grouting in Korea, and bentonite has usually been added to prevent the deposition of cement particles. The dispersion of CB(cement bentonite) grout is influenced by variable factors i.e. water to cement ratio, particle size of cement, kind of bentonite, adding volume, methods of adding, viscosity of CB grout materials and curdling time. Among variable factors, the viscosity of CB grout materials is influenced by the dispersion, and dispersion is improved as increasing the mixing speed. In this paper, described a suitable mixing speed of the High Speed Mixer in field, engineering characteristics of CB grout materials vary with the water to cement ratio and the mixing speed as well as confirming the state of dispersion.

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Effect of Different Seasons on Cross-Bred Cow Milk Composition and Paneer Yield in Sub-Himalayan Region

  • Sharma, R.B.;Kumar, Manish;Pathak, V.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.4
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    • pp.528-530
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    • 2002
  • The study was designed to evaluate the seasonal influences on cross-bred cow milk composition and paneer yield in Dhauladhar mountain range of sub-himalayan region. Fifty samples from each season were collected from a herd of $Jersey{\times}Red\;Sindhi{\times}Local$ cross-bred cows during summer (April-June), rainy (July-September) and winter (November-February) and analyzed for fat, total solids (TS) and solids not fat (SNF). Paneer was prepared by curdling milk at $85{\pm}2^{\circ}C$ with 2.5 per cent citric acid solution. Overall mean for fat, TS and SNF content of milk and paneer yield were 4.528, 13.310, 8.754 and 15.218 per cent respectively. SNF and TS content varied among seasons being highest in winter (8.983% and 13.639%) followed by summer (8.835% and 13.403%) and lowest in rainy season (8.444% and 12.888%). Paneer yield was lowest (14.792%) in rainy season and highest (15.501%) in winter season.

Physical Properties Variation of Grout Materials Based on the Water to Cement Ratio and the Mixing Speed (물시멘트비 및 혼합속도에 따른 주입재의 물성변화 연구)

  • Chun, Byung-Sik;Kim, Jin-Chun;Jang, Bui-Woong;Lee, Jun-Woo
    • Journal of the Korean GEO-environmental Society
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    • v.1 no.1
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    • pp.87-96
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    • 2000
  • Generally, OPC(ordinary portland cement) is used for grouting in Korea, and bentonite has usually been added to prevent the deposition of cement particles. The dispersion of CB(cement bentonite) grout is influenced by variable factors i.e. water to cement ratio, particle size of cement, kind of bentonite, adding volume, method of adding, viscosity of CB grout materials and curdling time. Among variable factors, the viscosity of CB grout materials is influenced by the dispersion, and dispersion is improved as the speed of grout mixer increase. In this paper, the specification of construction was derived by estimating physical characteristics of CB grout materials and confirming the sate of dispersion. The results show that the engineering characteristics of CB grout materials vary with the water to cement ratio and the mixing speed.

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The Visual Analysis of Myofascial Syndrome on Balance Posture (근육 근막 장애가 자세균형에 미치는 시각적 분석)

  • Park Young-Han
    • The Journal of Korean Physical Therapy
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    • v.9 no.1
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    • pp.177-184
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    • 1997
  • 1. The human body is the unification related to the powerful fascial network, I think. 2. Myofascial not only prevent and support the human body structure curdling but also keep the physical balance by dispersing traumatization properly. 3. Myofascial restriction will be developed into muscle deficiency and cause pains without releasing the muscle tension and the spasm. 4. Myofascial restriction affect and change the physical posture by losing the muscle elasticity and flexibility and by losing muscle supporting ability from gravitation. 5. The partial myofascial restriction affect the muscle and the adjoining joint supporting gravitation and cause the unbalance of the entire body.

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Whey Protein Attenuates Angiotensin II-Primed Premature Senescence of Vascular Smooth Muscle Cells through Upregulation of SIRT1

  • Hwang, Jung Seok;Han, Sung Gu;Lee, Chi-Ho;Seo, Han Geuk
    • Food Science of Animal Resources
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    • v.37 no.6
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    • pp.917-925
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    • 2017
  • Whey protein, a by-product of milk curdling, exhibits diverse biological activities and is used as a dietary supplement. However, its effects on stress-induced vascular aging have not yet been elucidated. In this study, we found that whey protein significantly inhibited the Ang II-primed premature senescence of vascular smooth muscle cells (VSMCs). In addition, we observed a marked dose- and time-dependent increase in SIRT1 promoter activity and mRNA in VSMCs exposed to whey protein, accompanied by elevated SIRT1 protein expression. Ang II-mediated repression of SIRT1 level was dose-dependently reversed in VSMCs treated with whey protein, suggesting that SIRT1 is involved in preventing senescence in response to this treatment. Furthermore, resveratrol, a well-defined activator of SIRT1, potentiated the effects of whey protein on Ang II-primed premature senescence, whereas sirtinol, an inhibitor of SIRT1, exerted the opposite. Taken together, these results indicated that whey protein-mediated upregulation of SIRT1 exerts an anti-senescence effect, and can thus ameliorate Ang II-induced vascular aging as a dietary supplement.

Effect of Fatty Acid Compositions by Monoglyceride on Rheological Properties of Ice Cream (Monoglyceride의 지방산(脂肪酸) 조성(組成)이 Ice Cream 물성(物性)에 미치는 영향)

  • Cho, Young-Koo
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.236-244
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    • 1988
  • The characteristics of ice cream, such as type-keeping viscosity, turbidity and stability of emulsion, were studied with the different composition of fatty acid of monoglyceride. The effect of saturated fatty acid of monoglycerides such as monolaurin, monomyristin, monopalmitin and monostearin on the characteristics of ice cream did not show any difference. The unsaturated fatty acid of monoglycerides, however, such as monocaprin and monoolein, was drastically enhanced the viscosity and easily happened the overrun of ice cream mixture which were resulted in the condensation of the fat droplet. Also the condensed fat droplet had the sterical network-structure. When the ratio of monostearin and monoolein becomed about 30:70, especially, it was confirmed the curdling of fat sphere increase to a maximun so that type-keeping and heat stability of ice cream were improved.

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