• 제목/요약/키워드: cultures

검색결과 4,684건 처리시간 0.028초

건강한 한국인 분변으로부터 분리된 Olsenella sp. KGMB 04489 균주의 유전체 염기서열 초안 (Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces)

  • 한국일;강세원;김지선;이근철;엄미경;서민국;박승환;이주혁;박잠언;오병섭;유승엽;최승현;이동호;윤혁;김병용;양승조;이정숙
    • 미생물학회지
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    • 제54권4호
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    • pp.456-459
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    • 2018
  • Olsenella 속 균주들은 척추동물의 구강, 반추위 및 분변 등에서 분리된 것으로 알려져 있다. 본 연구에서는 건강한 한국인 분변으로부터 Olsenella sp. KGMB 04489 균주를 분리하였으며 PacBio Sequel 플랫폼을 이용하여 Olsenella sp. KGMB 04489 균주의 유전체서열을 분석하였다. 유전체는 G + C 구성 비율이 65.5%이고, 1,838개의 유전자와 rRNA 13개, tRNA 52개로 구성되었으며, 염색체의 크기는 2,108,034 bp였다. 또한, 유전체 분석 결과를 통해 가수분해효소와 항생제 합성 및 내성과 관련된 다양한 유전자를 발견하였다.

1975년도(年度) 한국(韓國)에서 분리(分離)된 이질균(痢疾菌)에 관(關)한 보고(報告) (A Report on the Shigella Cultures Isolated in Korea(1975))

  • 김순희;전남호;류영해
    • 대한미생물학회지
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    • 제11권1호
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    • pp.23-26
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    • 1976
  • 1975年度 1년간(年間) 2033주의 장내병원성세균이 각 시, 도, 위생시험소 및 종합병원등에서 수집되어 검사대상이 되었으며 16주의 전형적인 이질균을 동정확인하였기에 미생물학적 성상을 보고하는 바이다. 수집된 지역적분포 사항은 Table 1과 같고 3 group으로, 6종류의 serotrpe으로 확인되었다. 생물학적 성상실험들은 미국 Center for Disease Control에서 발표된것과 비슷하였다. 우리나라에서 흔히 상용될 수 있는 항생제에 대한 감수성 검사를 실시하였으며 그 반응의 Pattern은 표3(Table 3)에서 보시는바와 같고 이 결과는 예년과 마찬가지로 Ericsson씨 법에 의해 관찰된 것이었다.

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Aspergllus oryza와 그 변이주의 연적배양에 의한 amylase 생성에 관한 연구 (Amylase Production by Continuous Cultures of Aspergillus oryzae and its Mutants)

  • Han, Hong-Eui
    • 미생물학회지
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    • 제15권2호
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    • pp.63-76
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    • 1977
  • Irradiation with high doses of gamma rays induced the reduction of mycelial weight and anaylase activity, and increased relative amylase activity in surface cultures. Biphase in growth curves was shown in aeration-agitation cultures but the behavior of the first phase of growth could be eliminated by replacing the amylasehydrolysed starch substrates, so that enzyme production was shortened ca. 40 hours and relative amylase activity was increased about 3 times higher before onset of autolysis. In the effect of gibberellin on amylase production, the positive stimulation was appeared to only surface culturs of the liquid medium and the negative effect to shake-cultures in a mutant. Trials of various continuous culture were resulted not only the approalch to the value of amylase activity in surface cultures of liquid medium, but also higher productivity than in batch cultures. The culture-degeneration was observed in two-stage continuous culture, but did not appear in continuous elevation culture.

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Effectiveness of Flashing Light for Increasing Photosynthetic Efficiency of Microalgal Cultures over a Critical Cell Density

  • Park, Kyong-Hee;Lee, Choul-Gyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제6권3호
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    • pp.189-193
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    • 2001
  • Critical cell density (CCD), the maximum cell concentration without mutual shading in algal cultures, can be used as a new operating parameter for high-density algal cultures and for the application of the flashing light effect on illuminated algal cultures. CCD is a function of average cell volume and light illumination area. The CCD is thus proposed as an index of estimation of mutual shading in algal cultures. Where cell densities are below the CCD, all the cells in photobioreactors can undergo photosysnthesis at their maximum rate. At cell densities over CCD, mutual shading will occur and some cells in the illumination chamber cannot grow photoautotrophically. When the cell concentration is higher than the CCD, specific oxygen production rates under flashing light were higher than those under continuous light. The CCD was found to be a useful engineering parameter for the application of flashing light, particularly in high-density algal cultures.

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Lactococcus lactis ATCC 11454로 처리한 냉장 돼지고기 등심의 미생물, pH 및 관능평가 (Microbiological, PH and Sensory Evaluations of Refrigerated Pork Loins Treated with Lactococcus lactis ATCC l1454)

  • 김창렬;김광현;이재일
    • 한국식품영양학회지
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    • 제14권2호
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    • pp.126-131
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    • 2001
  • Microbiological, pH and sensory evaluations of pork loins treated with lactic acid cultures (Lactococcus lactis subsp. lactis ATCC 11454 grown in 10% milk solids) during storage at 4$^{\circ}C$ were assessed. Pork loins were immersed in solutions containing 3.96 log units, 4.10 log units, or 4.23 log units of individual lactic acid cultures for 2 min. Pork loins treated with lactic acid cultures had a inhibitory effect (P<0.05) on the growth of aerobic palate counts during storage of 3 days at 4$^{\circ}C$. Pork loins treated with lactic acid cultures during storage of 3 days at 4$^{\circ}C$ significantly (P<0.05) decreased pH values compared to those of controls. Sensory evaluations for odor and appearance of pork loins treated with lactic acid cultures during storage of 9 days at 4$^{\circ}C$ were a "liked less" to "typical" category compared to those of controls.

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국내 패션잡지에 나타난 민족적 요소의 하이브리드 경향 (Hybridization of Ethnic-Cultural Elements Shown in Domestic Fashion Magazines)

  • 김선영
    • 복식
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    • 제61권8호
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    • pp.45-56
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    • 2011
  • This study is about the features of hybridization of ethnic-cultural elements in Korean fashion magazines. Its purpose was to embody more creative and newer images in fashion by promoting better mutual understanding of foreign cultures and addressing the issues of fashion design from multicultural perspectives. In doing so, the present study conducted a literature review and analyzed a total of 130 photographic images with any hybrid ethic element from two Korean fashion magazines, Vogue Korea and Harper's Bazaar Korea, issued between 2005 and 2009. The analysis revealed that there were 66 images(50.8%) with a mixture of two different cultures and 64 images(49.2%) with a mixture of three or more different cultures. As seen from the results, the two categories had a similar number of cases. In regional terms, the use of two different cultural elements included a mixture of Asian and Western cultures in 32 images(24.6%), a mixture of Western and African, Middle Eastern or Latin American cultures in 23(17.7%), and a mixture of Western and Russian or European folk cultures in 11(8.5%). In the use of three or more different cultural/national elements, the present study found a mixture of Asian, African, Middle Eastern, Latin American and Western costume items in 20 photo images(15.4%), a mixture of African, Middle Eastern and Latin American elements plus Western costume items in 19 images( 14.6%), a mixture of all regional cultures in 13 photos(10%), and a mixture of Asian ethic cultures plus Western costume items in 12 cases(9.2%). The results of this analysis indicated that the hybridization of ethnic-culture elements in Korean fashion magazines consisted of diverse clothing and accessories from various ethnic groups. The expression of these multi-cultural hybrid images that consists background images and models from different cultures well-portrayed the multi-cultural elements based on total coordination and broke the stereotypical aspects of styling.

Classification of Isolates Originating from Kimchi Using Carbon-source Utilization Patterns

  • LEE, JUNG-SOOK;CHANG OUK CHUN;MIN-CHUL JUNG;WOO-SIK KIM;HONG-JOONG KIM;MARTIN HECTOR;SAM-BONG KIM;CHAN-SUN PARK
    • Journal of Microbiology and Biotechnology
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    • 제7권1호
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    • pp.68-74
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    • 1997
  • One hundred and eighty two lactic acid bacteria, isolated mainly from kimchi, including reference strains were examined for their ability to utilize 95 carbon sources. The test strains were assigned to 5 major, 1 minor and 12 single-membered clusters based on the $S_{SM}$, UPGMA algorithm (at similarity of $80{\%}$). These aggregate clusters were equivalent to the genus Leuconostoc (aggregate cluster M and N), the genus Lactobacillus (aggregate cluster Q and R), and the genera Lactobacillus and Leuconostoc (aggregate cluster O and P) according to the database of the Biolog system. This study demonstrates that rapid identification and classification of isolates originating from kimchi can be achieved on the basis of such carbon source utilization tests.

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Attraction of the Invasive Hornet, Vespa velutina nigrithorax, by using Bacillus sp. BV-1 Cultures

  • Lim, Da Jung;Lee, Jeong Eun;Lee, Jin Sil;Kim, Iksoo;Kim, In Seon
    • 한국환경농학회지
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    • 제38권2호
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    • pp.104-109
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    • 2019
  • BACKGROUND: The invasive hornet Vespa velutina nigrithorax has becomes a public concern in rural and urban South Korea. The technologies are necessary to develop a way to counter V. velutina. In an effort to develop a way to counter V. velutina, we found that a bacillus strain, named Bacillus sp. BV-1, produces volatile compounds that attract V. velutina. METHODS AND RESULTS: Field trials of V. velutina attraction were performed using plates and traps containing BV-1 cultures grown on sugar medium. When the sugar medium and sugar-grown BV-1 cultures in the plates were placed close together, V. velutina visited preferably the plates with the BV-1 cultures. Significantly more V. velutina were caught in the traps containing BV-1 cultures than in those containing only sugar medium. Headspace solid-phase microextraction coupled with GC/MS analysis of BV-1 cultures detected 2-methyl-1-propanol, 3-methyl-1-butanol, 3-methylbutanoic acid, ethyl hexanoate, 2-pheylethanol, ethyl octanoate, and ethyl decanoate as the major volatiles. CONCLUSION: BV-1 cultures were suggested as potential agents for managing V. velutina as they produce volatile compounds that attract the hornet.

벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석 (Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation)

  • 박영희
    • 생명과학회지
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    • 제31권4호
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    • pp.385-388
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    • 2021
  • 벼 육종을 위한 약 배양은 기존 육종 방법을 사용하여 새로운 품종을 개발하는데 최소 6세대에 필요한 시간을 크게 줄여 동형접합자를 신속하게 생산하는 방법이다. 이 약 배양기술은 방법론적 관점에서 더 많은 녹색 식물을 얻을 기회를 제공하며, 약 배양에서 시간과 노력을 절약하는 배양의 효율성을 높이기 때문에 중요하다. 본 연구에서는 배양액과 배양 방법이 다른 1단계와 2 단계 배양의 캘러스 유도율과 녹색 식물 재분화율로 비교하였다. 1단계 배양은 하나의 배지에서 캘러스 유도 및 식물 재생을 허용하는 반면, 2 단계 배양은 두 개의 다른 배지에서 유도 및 식물 재분화가 필요하다. 이 연구에서는 1 단계 및 2 단계 배양으로 벼 꽃밥의 캘러스 유도와 식물 재생률을 비교했다. 캘러스 형성률은 1 단계 배양의 경우 13.0 %, 2 단계 배양의 경우 8.6%로 2 단계 배양보다 1 단계 배양에서 4.4% 더 높았다. 식물 재분화율은 1 단계 배양에서 1.0%, 2 단계 배양에서 3.0 %로 1 단계 배양보다 2단계 배양에서 식물체 재분화율이 3배 더 높았다. 이것은 2 단계 배양이 반수체 생산을 위한 1 단계 배양보다 더 효율적임을 시사한다.