• 제목/요약/키워드: culture-dependent

검색결과 1,207건 처리시간 0.022초

Nitrogen-Dependent Regulation of Gluconic and/or Citric Acid Production by Aspergillus niger

  • Sankpal, Narenora V.;Joshi, Arvino P.;Kulkarni, Bhaskar D.
    • Journal of Microbiology and Biotechnology
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    • 제10권1호
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    • pp.51-55
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    • 2000
  • Surface culture fermentation using Aspergillus niger was studied for gluconic and citric acid production at different C/N ratios. A culture of A. niger was found to produce either gluconic acid alone, a mixture of gluconic and citric acid, or citric acid alone depending on the level of nitrogen in the medium (4 to 18mM). Glucose oxidase from the mycelial mat was also analyzed at different levels of nitrogen in the media. By choosing the level of nitrogen in the medium at the start of fermentation, it is possible to produce either of the two acids as the dominant product or the two together as a mixture.

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조직시민행동과 선행요인간의 관계에 관한 연구 : TPM 제조혁신의 조절효과를 중심으로 (A Study on the Relationship Between Employees' Organizational Citizenship Behavior and Antecedents : Moderating Effect of TPM Manufacturing Innovation)

  • 최창호;이상곤;이재광
    • 지식경영연구
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    • 제10권3호
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    • pp.133-152
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    • 2009
  • The purpose of this study is to enhance an understanding of employee's OCB(organizational citizenship behavior) in manufacturing innovations like a TPM(total productive maintenance). OCB is examined as a dependent variable in three dimensions; compliance behavior, altruistic behavior, and innovative behavior. The six determinants of OCB are considered based on previous literature : organizational culture, team climate, transactional leadership, transformational leadership, job satisfaction, and organizational commitment. And the moderating effect of TPM on the relationships between OCB and determinants are examined. Data is collected through questionnaires and interviews from 684 employees in the Korean medium-sized manufacturing company that are conducting TPM. The result indicates that the important influencing factors of OCB are team climate, organizational culture, and job satisfaction. And the moderating effect of TPM on the relationships between OCB and job satisfaction is founded.

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Hypoglycemic Effect of Collybia confluens Exobiopolymer Produced by Submerged Mycelial Culture on Diabetic Rats

  • YANG, BYUNG-KEUN;LEE, HYUN-JI;JEONG, SANG-CHUL;LIM, WANG-JIN;SONG, CHI-HYUN
    • Journal of Microbiology and Biotechnology
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    • 제15권1호
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    • pp.136-140
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    • 2005
  • The hypoglycemic effect of Collybia confluens exobiopolymer was investigated in streptozotocin (STZ)-induced diabetic rats. In a dose-dependent study, the exobiopolymer, at 150 mg/kg body weight (BW) dose substantially lowered the plasma glucose level by 29.3%, as compared to the control group. It also lowered the plasma total cholesterol and triglyceride levels by 23.3 and 30.7%, respectively, and reduced the liver total cholesterol and triglyceride levels by 23.0 and 33.5%, respectively. The activity of alanine transaminase (ALT) and aspartate transaminase (AST) was reduced 34.6% and 23.6% respectively, by the exobiopolymer administration, compared to the control group. The exobiopolymer was found to contain 83.2% carbohydrate and 16.8% protein. The sugar and amino acid of the exobiopolymer were also analyzed in detail.

인터넷 패션 쇼핑몰에서 상호작용성과 쇼핑가치가 관계몰입과 웹사이트 태도에 미치는 영향 (Effect of Interactivity and Shopping Value on Relationship Commitment and Attitude toward the Website)

  • 이옥희
    • 복식문화연구
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    • 제16권6호
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    • pp.1126-1141
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    • 2008
  • The purposes of this study identify how attributes of the website impact on consumer attitude toward the website. For this purpose, the study tested covariance structural model which set relationships among independent variables (interactivity and shopping value), meditated variables(relationship commitment), and dependent variables(attitude toward website). The data were collected from a sample of 239 internet shopper of college female students. The covariance structural model and research hypothesis analyzed by using SPSS 16.0 and AMOS 5.0 program. The results are as follows: First, the structural model is accepted significantly. Second, interactivity and shopping value of website have a positive influence on relationship commitment. Third, interactivity, shopping value of website, and relationship commitment have a positive impact on attitude toward the website. Forth, even if shopping value has not a positive influence on attitude toward the website, it was found to have a significant effect on attitude toward the website through the relationship commitment.

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Effects of Baicalin on the differentiation and activity of preosteoclasts

  • Ko, Seon-Yle
    • International Journal of Oral Biology
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    • 제34권2호
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    • pp.81-86
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    • 2009
  • Baicalin is a flavonoid purified from the medicinal plant Scutellaria baicalensis. It has been reported that baicalin exhibits antibacterial, anti-inflammatory and analgesic effects. The present study was undertaken to determine the underlying cellular mechanisms of baicalin action in preosteoclasts. The effects of this flavonoid on preosteoclasts were determined by measuring osteoclast generation and osteoclast activity in macrophage-colony stimulating factor (M-CSF)-dependent bone marrow cells (MDBMCs) and in co-cultures of MDBMCs and osteoblasts. Osteoclast generation was assayed by measuring the number of tartrateresistant acid phosphatase (TRAP) (+) multinucleated cells after culture. Osteoclast activity was assayed by measuring the area of the resorption pit after culture. We found that osteoclast generation was induced by M-CSF and receptor activator of NF-kB ligand (RANKL), and by the 1.25-dihydroxycholecalciferol in our cultures. Baicalin decreased both osteoclast generation and activity in MDBM cultures and co-cultures indicating that it may inhibit bone resorption.

재가노인들을 위한 무료급식소의 식단 평가 (Evaluation of the Menus of Free Meal Service Centers for Home-bound Elderly)

  • 한경희;박정숙;최미숙;정순둘;채인숙
    • 한국식생활문화학회지
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    • 제17권5호
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    • pp.584-593
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    • 2002
  • The purpose of this study was to evaluate the diet quality of the menus delivered by 17 free meal service centers for the low-income home-bound elderly in Chung- cheong buk-Do. Statistical data analysis was compleleted using the SPSS package program for descriptive analysis, T-test, and ANOVA. The meals offered by free meal service centers were not met the 1/3 recommended dietary allowances in calcium and vitamin $B_2$. There were significant differences between dependent variables(nutrient content, nutrient density, nutrient deficiency, NAR, MAR, food group intake patterns) and independent variables (operation type, operation status, operation period, nutritionist, food cost).

패밀리 레스토랑의 Yield Management 전략에 대한 고객의 태도 (Family Restaurant Customers' Attitude toward Yield Management Strategies)

  • 박희경;손대현
    • 한국식생활문화학회지
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    • 제20권2호
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    • pp.243-252
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    • 2005
  • This study identified how family restaurant customers' attitude toward yield management strategies, which include reservation, time, menu, and service factors. Generally, yield management explains how price and dining duration maximize the revenue of family restaurants. There are three ways to improve family restaurant yield; improving table turns, upgrading seat occupancy, increasing price per customer. The 45 items of yield management strategies are developed by literature reviews, web sites analysis, and interviews with family restaurant employers. Factor analysis and reliability analysis from present study found out 11 important factors, which represent the study's dependent variables. Regression analysis may be helpful to test whether demographic and socio-economic factors have relationships with family restaurant customers' attitude toward yield management strategies. As a regression result, gender, family size, age in demographic factors and education and occupation in socio-economic factors have relationships with family restaurant customers' attitude toward yield management strategies significantly.

Mercury chloride 및 Methylmercury chloride가 정상인(正常人)의 혈액배양(血液培養)에서 임파구(淋巴球)의 자매염색분체교환(姉妹染色分體交換)에 미치는 영향(影響) (Sister Chromatid Exchanges in Lymphocytes on Normal Human Blood Culture with Mercury chloride or Methylmercury Chloride)

  • 고대하
    • Journal of Preventive Medicine and Public Health
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    • 제17권1호
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    • pp.245-250
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    • 1984
  • Reciprocal exchanges of DNA in sister chromatids (SCEs) are induced by various carcinogens and mutagens, although the quantitative relationship between the number of mutations and SCEs induced varies among chemicals. Nevertheless, the analysis of SCEs production by various agents often proposed as a sensitive and quantitative assay for mutagenicity and cytotoxicity. Mercury, even if which has no evidences for mutagenicity and carcinogenicity, is reported to exert some cytotoxic effects, such as chromosomal aberrations or bad influences to ovulation and reproduction in experimental animals, etc.. In this study, tests for sister chromatid exchanges have been carried out on normal human lymphocytes in whole blood culture to add mercury chloride ($HgCl_2$) or methylmercury chloride ($CH_3\;HgCl$) for 72 hr. The results indicate the dose-dependent relationship between the frequencies of SCEs and the concentrations of $HgCl_2,\;CH_{3}HgCl$ and 5-bromo-2'-deoxyuridine (BrdU). Lymphocyte proliferation has depressed in the higher concentration of mercury.

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Bacterial Diversity in a Korean Traditional Soybean Fermented Foods (Doenjang and Ganjang) by 16S rRNA Gene Sequence Analysis

  • Cho, Kye-Man;Seo, Weon-Taek
    • Food Science and Biotechnology
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    • 제16권2호
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    • pp.320-324
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    • 2007
  • The bacterial diversity in Korean soybean-fermented foods was investigated using a PCR-based approach. 16S rRNA sequences were amplified and cloned from two different soybean-fermented foods such as doenjang (soybean paste), and ganjang (soybean sauce). Staphylococcus equorum (60.6%), Tetragenococcus halophila (21.2%), Leuconostoc mesenteroides (9.1%), Lactobacillus sakei (6.1%), and Bacillus subtilis (3.0%) were detected among clones isolated from soybean paste samples and Halanaerobium sp. (37.5%), Halanaerobium fermentans (37.5%), T. halophila (12.5%), Staphylococcus sp. (6.3%), S. equorum (3.1%), and B. subtilis (3.1%) were detected among clones isolated from soybean sauce. Our approach revealed different bacterial distributions and diversity from those previously obtained using culture-dependent methods.

Factors Affecting the Development of Embryos Produced by Nuclear Transfer

  • Lee, Joon-Hee;Campbell, Keith H.S.
    • 한국수정란이식학회지
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    • 제27권4호
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    • pp.193-203
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    • 2012
  • The development of embryos reconstructed by nuclear transfer is dependent upon numerous factors including the type of recipient cell, method of enucleation, the type of donor cell, method of reconstruction, activation, the cell cycle stage of both the donor nucleus and the recipient cytoplasm and the method of culture of the reconstructed embryos. Many of these points which have been reviewed extensively elsewhere (Sun and Moor, 1995; Colman, 1999; Oback and Wells, 2002; Renard et al., 2002; Galli et al., 2003b), here we will concentrate on main area, the production of suitable cytoplast and nuclear donor, nuclear-cytoplasmic coordination, oocyte activation, culture of reconstructed embryos, and the effects that this may have on development.