• 한국미생물·생명공학회지
• /
• 제51권1호
• /
• pp.10-17
• /
• 2023

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 10⁷ colony-forming unit (CFU)/ml for fermented milk products and 10⁸ CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

• Journal of Species Research
• /
• 제6권1호
• /
• pp.68-75
• /
• 2017

We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LEDs lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LEDs light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권10호
• /
• pp.1550-1554
• /
• 2003

One hundred of lactic cultures were evaluated for their ability to inhibit hazardous microbes, such as Salmonella enteritidis, Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Bacillus cereus by agar well diffusion method. None of them showed inhibitory halo against S. enteritidis, while 27 strains showed inhibitory activity against S. typhimurium, 6 against E. coli, 9 against ampicillin resistant E. coli, 31 against L. monocytogens, 10 against B. cereus. pH of the culture does not explain for the inhibitory activity except against B. cereus. A neutralized culture from corn silage showed highest inhibitory activity against S. typhimurium, and the size of inhibitory halo was same as 10 ug/mL of ampicillin. The culture was identified to be Lactobacillus buchneri on the basis of biochemical characteristics and utilization of substrates. Using the culture as probiotics could be expected to reduce antibiotics for animal feeding.

• 감성과학
• /
• 제13권4호
• /
• pp.741-752
• /
• 2010

본 연구는 매력적인 도시브랜드개성 창출과 이의 활용을 위해, 도시의 문화자원의 도시브랜드개성에 미치는 영향을 규명하고, 관광객과의 호의적인 관계 구축 및 충성도를 이끌어내기 위한 방안을 도출하고자 시도되었다. 실증분석결과는 다음과 같이 요약된다. 첫째, 도시 문화자원은 문화프로그램, 문화인프라, 문화인력으로 구성된다. 둘째, 도시브랜드개성은 혁신성, 평온함, 세련성, 신뢰성, 역동성으로 각각 분류되었다. 셋째, 도시의 문화자원은 도시브랜드개성 형성에 정(+)의 영향을 주며, 각각 상의한 역할을 하는 것으로 나타났다. 넷째, 도시브랜드개성은 도시브랜드만족도와 도시브랜드몰입도에 각각 정(+)의 영향을 준다. 다섯째, 도시브랜드 만족도와 도시브랜드몰입도는 도시브랜드 충성도에 각각 정(+)의 영향을 준다. 이와 같은 결과는 효과적인 도시의 문화자산은 독특한 도시브랜드개성을 창조하며, 지속가능한 경쟁 우위 원천으로써, 도시의 매력을 효과적으로 전달하는 커뮤니케이션도구임을 시사한다.

• 한국자원식물학회지
• /
• 제3권1호
• /
• pp.1-30
• /
• 1990

The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

• 한국병원경영학회지
• /
• 제20권1호
• /
• pp.1-12
• /
• 2015

This study aims to examine the effect of a change of leadership on internal environment and organization effectiveness and suggest measures on effective organizational management by analyzing a change of leadership before and after 2nd generation management. There was analysis of a difference of leadership type, organizational structure, organizational culture, management resources, job satisfaction, and organizational commitment between the period of the founders and the period of 2nd generation successors by collecting data from employees of 6 hospitals in Seoul. Also there was analysis of the effect of a change of leadership on internal environment and organizational effectiveness. According to the analysis, it was demonstrated that the factor affecting job satisfaction during the period of the founder was complexity of organizational structure, and there were no factors affecting organizational commitment. In contrast, during the period of the 2nd generation successors, it was revealed that transformational leadership, centralism of organizational structure, and human resources of management resources affect job satisfaction, and transformational leadership, complexity of organizational structure, hierarchical culture of organizational culture, and human resources of management resources affect organizational commitment. Further, after the succession from the founders to 2nd generation successors, as transformational leadership increased, job satisfaction rose, and as developmental culture was reinforced and human resources increased, organizational commitment was heightened. Furthermore, as transformational leadership increased and developmental culture was reinforced, organizational commitment was heightened. Namely, as transformational leadership was reinforced resulting from the succession to 2nd generation, employees' job satisfaction and organizational commitment increased, with circumstances aiming for the actual change.

• 한국버섯학회지
• /
• 제2권3호
• /
• pp.140-144
• /
• 2004

전통적으로 Cordyceps종은 한국을 포함한 동양에서 건강증진과 심장과 폐에 관련된 질환을 치료하는 약용식물의 하나로 사용되어왔다. 최근에 동충하초의 약리적 가치때문에 큰번데기 동충하초를 포함한 몇가지 Cordyceps 종의 인공재배에 관한 연구가 활발하게 이루어지고 있다. 본 연구에서는 큰번데기 동충하초를 in vitro에서 인공재배한 결과 자실체 형성이 불안정한 것을 관찰하였기에 보고하고자 한다.

• Journal of Microbiology and Biotechnology
• /
• 제28권1호
• /
• pp.32-39
• /
• 2018

Samples of Laru (a fermentation starter) obtained from the upper part of Borneo Island were analyzed for their lactic acid bacteria (LAB) and fungal diversity using both a culture-independent method (PCR-DGGE) and culture-dependent methods (SDS-PAGE and MALDI-TOF MS). Pediococcus pentosaceus, Lactobacillus brevis, Saccharomycopsis fibuligera, Hyphopichia burtonii, and Kodamaea ohmeri were detected by all three methods. In addition, Weissella cibaria, Weissella paramesenteroides, Leuconostoc citreum, Leuconostoc mesenteroides, Lactococcus lactis, Rhizopus oryzae/Amylomyces rouxii, Mucor indicus, and Candida intermedia were detected by PCR-DGGE. In contrast, Lactobacillus fermentum, Lactobacillus plantarum, Pichia anomala, Candida parapsilosis, and Candida orthopsilosis were detected only by the culture-dependent methods. Our results indicate that the culture-independent method can be used to determine whether multiple laru samples originated from the same manufacturing region; however, using the culture-independent and the two culture-dependent approaches in combination provides a more comprehensive overview of the laru microbiota.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2003년도 생물공학의 동향(XII)
• /
• pp.245-248
• /
• 2003

As a result of broth substitutions when each culture-mediums were difference, whole culture-medium was found to be best feeding solution for production of PS-7 by B. indica. Maximal production of PS-7 was 1$10.0\;g/{\ell}$ and its conversion rate from 2% (w/v) glucose to PS-7 was 50%. After 48 hr, 50%(v/v) medium of working volume began to substitute in 7L jar fermenter. Production of PS-7 increased after 48hr, recovered productivity of PS-7. Following this preliminary culture, the resultant culture was subjected to continuous flow conditions controlled that the dilution rate were $0.01\;{\sim}\;0.04\;h^{-1}$. Production of PS-7 increased at dilution rate $0.0100\;h^{-1}$ whereas productivity of PS-7 decreased gradually in dilution rate $0.0200\;{\sim}\;0.0400\;h^{-1}$. Maximal production of PS-7 was $10.0\;g/{\ell}$ in continuous culture.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2001년도 추계정기 학술발표회 초록집
• /
• pp.111-113
• /
• 2001