• International Journal of Advanced Culture Technology
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• 제11권4호
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• pp.263-272
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• 2023

We investigated and analyzed students' perceptions of their choice of science subjects in the high school credit system and the reasons for their choice. To achieve this, the selection of science subjects was investigated for the second year of S high school over the past three years. Students selected an average of 1.54 science subjects, and it was found that the rate of selection of science subjects was gradually increasing by year. Students chose high in the order of life science I, earth science I, chemistry I, physics I, etc. in the science subject group. Students who wish to enter the natural and engineering fields chose life science I, chemistry I, physics I, etc., while, students who wish to enter the humanities society chose society and culture, life science I, ethics and thought, etc. On the other hand, the reason for choosing science subjects was 'related to college admission', followed by 'aptitude and interest', 'career and real life help', etc. physics I, chemistry I, etc., were high in the subjects selected according to the 'related to college admission'. The subjects selected according to 'aptitude and interest' were high in life science I, earth science I, etc. Physics I, chemistry I, etc. are recognized as subjects necessary for college entrance, and life science I, earth science I, etc., are found to be related to their interests and aptitudes.

• 한국보건간호학회지
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• 제26권1호
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• pp.5-15
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• 2012

Purpose: The effects of the organizational culture and health promotion life style on job satisfaction of nurses in general hospitals have been studied in an effort to provide basic information that will be helpful in effective management of the organization and enhancement of nursing. Method: The 341 samples used in the study were selected randomly from general hospitals having more than 250 beds in Gwangju province and Yosu province. Data collected were analyzed for frequency, percentage, ANOVA, t-test, Pearson's correlation analysis, and Multiple regression analysis using SPSS, a program package for statistical analysis. Result: Factors affecting job satisfaction included work years, position, monthly income, stress management, which is a substructure of health promotion life style, innovation-oriented culture, relation-oriented culture, and task-oriented culture in organizational culture. Results showed an influence of 64.2%. Ed - highlight: Please review. Conclusion: The organizational culture, such as an innovation-oriented culture, relation-oriented culture, or task-oriented culture, has a positive influence on job satisfaction.

• International Journal of Advanced Culture Technology
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• 제7권2호
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• pp.67-76
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• 2019

As the attracting foreign student has become a very important strategy, detailed researches on their college selection and their satisfaction with college life are needed. Especially it is necessary to distinguish ordinary international students and those who take classes in Local Education Centers (LEC) before coming to Korea. The central purpose of this study is to identify how the two types of students differ in their perception of college selection factors and what factors affect their satisfaction with college life. A total of 186 international students participated in the study. It was found that the most important college selection factor of the pre-educated LEC students was 'obtaining academic ability through online classes and transfer of credit hours'. Second, these students reported that the two influential factors for their satisfaction with college life were 'quality of education' and 'cultural experience program'. Third, it was found that the major college selection factors influencing ordinary international students' college life satisfaction were 'expertise of faculty', 'transfer of credit through curriculum links', and 'recommendation from teachers at home country'. Fourth, the major factors affecting the pre-educated LEC students' satisfaction with college life were 'KSL classes at LE's', 'expertise of faculty', and 'financial aid system'.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.14-22
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• 2019

This study is intended to look into the effects of depression and stress on college life adjustment of ophthalmic optics students. To achieve this, a survey was empirically carried out to 142 male and female students majoring in ophthalmic optics at colleges in Gangwon-do. The findings are as follows: Depression had a significant effect on academic adjustment and social adjustment, but no significant effect on emotional adjustment and college satisfaction. Stress also had a significant effect on academic adjustment, but no significant effect on social adjustment, emotional adjustment and college satisfaction. The above results showed that depression and stress were major factors affecting academic and social adjustments, and academic adjustment, respectively. Which means that depression restricted the activities of academic adjustment and social adjustment, and did not have a close relationship with psychological mechanisms - emotional adjustment and college life satisfaction. Stress also had a significant effect on academic adjustment, but did not have a direct relationship with social adjustment, emotional adjustment and college satisfaction.

• Journal of Veterinary Science
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• 제21권1호
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• pp.9.1-9.15
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• 2020

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 춘계학술대회
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• pp.147-147
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• 2018

• 생명과학회지
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• 제26권6호
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• pp.633-639
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• 2016

고분자(polymer), 금속(metal), 세라믹(ceramic), 합성물(composite) 등과 같은 바이오소소재(Biomaterials)는 그들의 물리화학적 성질 때문에 의료용섬유(medical fibers), 인공혈관(artificial blood vessels), 인공관절(artificial joints), 임플란트(implants), 연조직(soft tissue), 인공성형물(plastic surgery materials) 등 의료용으로 많이 사용되며, 개발연구도 활발히 진행되고 있다. 그러나, 필름(film)이나 판(plate)형태의 바이오소재에 대한 생체적합성(biocompatibility)이나 독성(toxicity)을 포유동물세포를 이용하여 평가하는 것은 적절한 평가용 장치가 없기 때문에 매우 어려운 상황이다. 따라서 이러한 문제를 해결하기 위해서, 본 연구에서는 고분자필름이나 금속판에 유용하게 적용할 수 있는 실리콘링, 상판(top panel), 하판(bottom panel)으로 구성된 새로운 포유동물배양시스템을 개발하고, 이를 실제 적용하고자 하였다. 개발된 시스템은 평가하고자 하는 시료를 상판과 하판사이에 조립하는 샌드위치시스템을 기반으로 한다. 세포배양장치의 조립 후, SK-MEL-2세포를 3가지 시료; Styela Clava Tunic (SCT)- PF, NaHCO₃-added SCT (SCTN)-PF, magnesium MP (MMP)에 적용하고 37℃ 이산화탄소 배양기에서 24시간과 48시간 동안 배양하였다. MTT분석결과에서, 세포생존율(cell viability)은 24시간과 48시간 동안 SCT-PF배양그룹에서 정상적으로 유지되었지만 48시간 동안 SCTN-PF배양그룹에서는 급격하게 감소되었다. 더불어, MMP배양그룹에서 세포생존율은 24시간과 48시간 배양 후에 대조군과 유사하게 유지되었다. 이러한 결과는 본 연구에서 새롭게 개발된 샌드위치형태의 포유동물세포배양장치는 고분자필름이나 금속판형태의 바이오소재에 대한 독성이나 생체적합성을 평가하기 위한 우수한 잠재력을 보유하고 있음을 제시하고 있다.

• International Journal of Advanced Culture Technology
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• 제4권4호
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• pp.13-22
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• 2016

The purpose of this study is to identify the characteristics of school life and to examine the influencing factors of academic adjustment and social adjustment in university students. This cross-sectional descriptive study by using secondary data from 2,064 subjects who responded to a self-rated student adjustment test. Descriptive, t-test, ANOVA, Pearson correlation and multiple regression analyses were conducted. Satisfaction with college selection, expectation for college, satisfaction with college service, emotional stability, and relationships with faculty were significantly higher among male students, whereas confidence in major, support from others were significantly higher among the female students. There was a significant positive relationship between academic adjustment and confidence in major, and between social adjustment and expectation for college. Confidence in major, relationships with faculty, emotional stability, expectation for college, satisfaction with college selection, and commitment to college life accounted for 31.8% of the variance in academic adjustment. In addition expectation for college, emotional stability, support from others, gender, commitment to college life, and satisfaction with college services accounted for 44.7% of the variance in social adjustment for university students. Overall, the results of this study suggest that understanding the levels of expectation for college, commitment to college life, and levels of emotional stability may be important for facilitating their transition and adjustment to university life.

• 생명과학회지
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• 제27권5호
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• pp.567-574
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• 2017

본 연구는 막걸리로부터 ${\gamma}$-amino butyric acid (GABA) 생성 유산균을 분리 및 동정하고 최적 GABA 생산조건을 확립하는데 그 목적이 있다. 막걸리로부터 64균주의 유산균은 MRS 배지에서 성장된 집락의 색과 모양의 특성에 따라 분리하였다. 분리균주의 GABA 생산은 1% MSG가 첨가된 MRS 액체배지에서 배양하여 TLC와 HPLC 방법에 의해 평가되었다. B-134 균주는 GABA생성을 위한 우수균주로 선발하였다. 16S rRNA 유전자 및 glutamate decarboxylase B (gadB) 유전자의 염기서열분석을 통하여, B-134 균주는 Lactobacillus plantarum subsp. plantarum B-134 균주로 명명하였다. GABA 생성을 위한 온도, pH, NaCl 및 MSG 농도를 달리하여 최적배양조건을 조사하였다. 그 결과 B-134 균주의 최적배양 조건은 온도 $37^{\circ}C$, pH 5.7, NaCl 농도 0% (w/v), 그리고 MSG 농도 3% (w/v)로 결정되었으며 본 조건에서 48시간 배양시 25 mM의 GABA를 생산하였다. 이러한 결과로부터 B-134 균주는 GABA함유 건강기능식품개발을 위한 유용한 균주로 판단된다.

• Reproductive and Developmental Biology
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• 제38권4호
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• pp.147-158
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• 2014

Differentiated nuclei can experimentally be returned to an undifferentiated embryonic status after nuclear transfer (NT) to unfertilized metaphase II (MII) oocytes. Nuclear reprogramming is triggered immediately after somatic cell nucleus transfer (SCNT) into recipient cytoplasm and this period is regarded as a key stage for optimizing reprogramming. In a recent study (Dai et al., 2010), use of m-carboxycinnamic acid bishydroxamide (CBHA) as a histone deacetylase inhibitor during the in vitro early culture of murine cloned embryos modifies the acetylation status of somatic nuclei and increases the developmental competence of SCNT embryos. Thus, we examined the effects of CBHA treatment on the in vitro preimplantation development of porcine SCNT embryos and on the acetylated status of histone H3K9 on cloned embryos at the zygote stage. We performed the three groups SCNT: SCNT (NT), CBHA treatment at the porcine fetus fibroblast cells (PFFs) used as donor cells prior to SCNT (CBHA-C) and CBHA treatment at the porcine SCNT embryos during the in vitro early culture after oocyte activation (CBHA-Z). The PFFs were treated with a $15{\mu}M$ of CBHA (8 h) for the early culture and the porcine cloned embryos were treated with a $100{\mu}M$ concentration of CBHA during the in vitro early culture (10 h). Cleavage rates and development to the blastocyst stage were assessed. No significant difference was observed the cleavage rate among the groups (82.6%, 76.4% and 82.2%, respectively). However, the development competence to the blastocyst stage was significantly increased in CBHA-Z embryos (22.7%) as compared to SCNT and CBHA-C embryos (8.6% and 4.1%)(p<0.05). Total cell numbers and viable cell numbers at the blastocyst stage of porcine SCNT embryos were increased in CBHA-Z embryos as compared to those in CBHA-C embryos (p<0.05). Signal level of histone acetylation (H3K9ac) at the zygote stage of SCNT was increased in CBHA-Z embryos as compared to SCNT and CBHA-C embryos. The results of the present study suggested that treatment with CBHA during the in vitro early culture (10 h) had significantly increased the developmental competence and histone acetylation level at the zygote stage.