• 한국어교육
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• 제28권3호
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• pp.127-152
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• 2017

The purpose of this study is to clarify the perception and educational needs of international college students regarding the culture of college life. A questionnaire was given to international college students who are studying at universities in Korea. The results showed that international college students need to study about the culture of college life and had a perception that it is desirable to learn this through general education classes or freshman subjects after entering university. Also, the culture of college life can be divided into nine categories: bachelor's system; career and employment; university support centers and systems; interpersonal relations; departmental events; housing and living expenses; freshmen's events; exchange activities and programs; and cultural experience activities. International college students showed higher learning needs in order of career and employment; cultural experience activity; and bachelor's system. In addition, the characteristics of the learners showed different educational needs according to year of study and Korean language proficiency. The significance of this study is to specify the items of the culture of college life required for international college students, to measure the validity and reliability of these items, and to categorize the elements of the culture of college life.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.212-212
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• 2017

This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

• 한국수정란이식학회지
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• 제29권3호
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• Journal of Plant Biotechnology
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• 제34권4호
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• pp.355-361
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• 2007

Cotyledons of perilla6 were cultured on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BA for 7 weeks. The activity of perilla peroxidase was observed to increase following culture stages as assessed by peroxidase assay. A peroxidase (POD) was purified from perilla tissue cultured on MS medium for 7 weeks. The peroxidase was purified using ion exchange and gel nitration chromatography. The perilla peroxidase had a molecular mass of 30 kDa by SDS-PAGE. We showed that the N-terminal amino acid sequence of this protein shared 67% identity with the tea peroxidase. As indicated by SDS-PAGE, the banding pattern of the 30 kDa polypeptide present in total soluble protein from perilla tissue was increased following culture stages. Immunoblot analysis indicated that perilla peroxidase protein appeared after 3 weeks of perilla tissue culture, and continued to increase with extended duration of tissue culture for at least 7 weeks.

• 한국자원식물학회지
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• 제19권2호
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• pp.227-231
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• 2006

본 연구는 2종류의 하우스, 즉 1-2W 형 하우스와 무기둥하우스에, 시설억제재배, 촉성계배, 반측성재배 그리고 여름재배의 4 재배시기에 8개의 오이 품종을 재식하여, 재배시기에 따른 품종들의 암수꽃의 발생, 개체 당 과실수에 따른 퇴화 과실수, 그리고 수량을 조사 비교하여 각 작형에 알맞은 품종들을 추천하기 위해 수행하였다. 시설억제재배에서는 낙합계나 흑진주계 또는 입추백다다기오이가 생육 및 수량이 양호하였고, 촉성재배에서는 겨우살이청장오이, 입추백다다기오이 그리고 가을낙합오이가 추천된다. 반측성재배에서는 청장계 오이나 낙합계 오이가 비교적 양호한 수량을 보였으며 여름재배에서는 남부청장오이나 가을낙합오이가 양호하였다.

• 임상간호연구
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• 제17권1호
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• pp.57-69
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• 2011

Purpose: This study aimed to investigate the types of nursing organizational culture and its relationships with nurses' satisfaction with life, and job satisfaction. This study was eventually aimed to create healthier and more positive nursing organizational culture. Methods: This study utilized a descriptive correlational method to explore mediating effects of the types of nursing organizational culture on nurses' satisfaction with life and their job. Data were collected from a total of 1,801 nurses who have worked for more than 3 years in 35 different hospitals with more than 100 beds. Using SPSS 17.0 and Sobel Test Calculator, t-test, one way ANOVA, Duncan test, multiple regression and sobel test were used for data analysis. Results: The analysis showed that the nurses had a hierarchical organizational culture. Also the subjects' job satisfaction was significantly correlated with innovation, relation, and hierarchy-oriented factors and satisfaction with life. Conclusion: Innovative organizational culture is a leading factor in determining nurses' professional satisfaction. In view of the above it is recommended that strategies for creating innovative organizational culture and its application to nursing practice.

• 한국약용작물학회지
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• 제20권6호
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• pp.461-465
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• 2012

This study was carried out to select the appropriate medium (especially, carbon and nitrogen source, potassium phosphate and pH) for somatic embryogenesis in order to develop the rapid mass production system in suspension culture of Oplopanax elatus Nakai. Direct somatic embryos were obtained from root explants in the hormone free suspension culture (MS). Combination of $NH_4NO_3$ and $KNO_3$ at the ratio of 1650 (mg/${\ell}$) : 1900 (mg/${\ell}$) obtained the better result to produce somatic embryo in suspension culture. MS medium supplemented with $170mg/{\ell}$ $KH_2PO_4$. The addition of 1 and 3% sucrose was effective for formation of embryogenic callus. Therefore, this report will be helped to improve the establishment for suspension culture in Oplopanax elatus Nakai.

• 국제교류와 융합교육
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• 제2권2호
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• pp.59-79
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• 2022

In recent years, "Korean Wave" has entered the life of college students with the carrier of popular culture such as popular music, TV plays and novels, which is unique to South Korea, and has been widely pursued by college students. The Korean wave culture inevitably exerts an important influence on their values. Based on this, on the basis of questionnaire survey, this paper takes Chinese college students as the research object. After understanding the attitude and contact status of contemporary college students towards Korean Wave culture, this paper explores the reasons for the prevalence of Korean Wave culture in China, and then specifically analyzes the influence of Korean Wave culture on Chinese college students from both positive and negative aspects. It also puts forward countermeasures and suggestions to correctly guide college students to face the Korean Wave culture from the four aspects of society, colleges, families and individuals. I hope to guide college students to rationally cope with the Korean wave culture, establish a correct outlook on life, and better integrate into the trend of The Times.

• International Journal of Industrial Entomology and Biomaterials
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• 제35권1호
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• pp.58-62
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• 2017

The two-spotted spider mite (Tetranychus urticae) has sustained damage on more than 200 host plants worldwide. Many farmers have relied on chemical acaricides to control mite, but the abuse of acaricides has caused serious resistance to mite. To overcome this problem, microbial control using entomopathogenic fungi have been studied. Entomopathogenic fungi have been an important role against the control of pest, and most of their culture products have been demonstrated to have virulence against pest population. In this study, we evaluated and compared the virulence of culture filtrates, aerial conidia and blastospores of selected Metarhizium anisopliae 4-2 and Beauveria bassiana 2R-3-3-1, respectively, among two-spotted spider mite-pathogenic fungi. As a result, the virulence was confirmed in all treatments, and the accumulated mortality rates were between 77 and 100% within 7 days. Especially, treatment with the fungal culture filtrate alone exhibited quite high virulence, and combined treatment with aerial conidia or blastospores enhanced activity. However, the median lethal time of treatments was not significantly different. When two isolates were compared, M. anisopliae 4-2 showed higher virulence than B. bassiana 2R-3-3-1. These results suggest that the selected two fungal isolates and their culture products could be used effectively for the control of two-spotted spider mite.

• Reproductive and Developmental Biology
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• 제38권3호
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• pp.93-98
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• 2014

The objective of this study was to investigate the changes of oxidative stress and antioxidant enzyme during in vitro development with washing culture oil in porcine embryos. During the culture, the four types of culture oil such as paraffin oil with or without washing and mineral oil with or without washing were examined. The oil was washed with PZM-3 during 7 days and collected oil only. The embryos were stained with CellTracker$^{TM}$ Red, DCFDA and Hoechst 33342 to confirm the effects of the oil. As a results, Cleavage rates and total cell number were no difference among the four oil groups. However, ${\geq}16$ cell embryos were significantly different in fore type oil treatment and blastocyst rate was significantly higher washing paraffin treatment than in other group(p<0.05). Also, the expression of free radical were lower in washing paraffin oil than in other groups (p<0.05). On the other hand, the expression of glutathione were not significant different among paraffin oil with or without washing and mineral oil with or without washing, however washing paraffin oil and washing mineral groups were higher than other treatment groups. In conclusion, the washing oil was expected with positive effects on in vitro development in porcine embryos.