• Title/Summary/Keyword: culture method

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Current Status and Issues of Nuclear Safety Culture

  • Lee, Yong-Hee
    • Journal of the Ergonomics Society of Korea
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    • v.35 no.4
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    • pp.247-261
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    • 2016
  • Objective:The aim of this paper is to discuss the nuclear safety culture in Korea. Background: Recently, Korean nuclear field has met a strong anti-nuclear trend after a few happenings, as well as Fukushima accident, which are turned out to be caused by organizational pitfalls and safety culture defects in general. Method: We review the concepts and constructs of safety culture based on the brief history of the nuclear safety culture that IAEA mainly raised after the Chernobyl accident in 1986. Additionally, we give a comparative discussion on the approach to the safety culture with U.S. Results: Finally, we discuss the three technical domains and propose six selected domestic issues related to the nuclear safety culture for coping with the demanding requests on nuclear safety in Korea. Conclusion and Application: We suggest several recommendations and a research direction in the form of a re-start line for the nuclear safety culture in Korea.

Recombinant Human Parathyroid Hormone Related Peptide (1-34) Stimulates Osteoclastic Bone Resorption in Both Rodent and Avian Disagsresated Osteoclast Culture (파골세포배야에서 나타난 부갑상선호르몬의 설치류 및 조류 파골세포에 대한 촉진 효과)

  • 양대석;김일찬남궁용이창호
    • The Korean Journal of Zoology
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    • v.37 no.2
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    • pp.255-261
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    • 1994
  • Recombinant human pBrathyriod hormone related peptide (1-341 (rhPTHrP) has been known to stimulate bone resorption in intact bone tissue culture system. Osteoclast has been known as a primary responsible cell for bone resorption. To examine the effect of rhPTHrP on this cell, we employed disaggregated rat osteodast culture. As a result, we found that rhPTHrP sisnificBntly elevates both the number and total area of resorbed pits in this culture. On the other hand, the conflicting results between disagsregated rat osteoc13st culture and Ca2+-deficient hen osteoclast culture system have been a big obstacle for the progress of bone research. To verify the differences between rat 3nd chick osteoclast system, we performed the same experiment using chick embryonic osteoclast. Since the similar results were obtained from the disaggregated chick osteoclast culture, the discrepancy between chick and rat osteoclast culture study seemed to be due to the difference in culture method, rather due to the species-difference.

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Improved Collaborative Filtering Using Entropy Weighting

  • Kwon, Hyeong-Joon
    • International Journal of Advanced Culture Technology
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    • v.1 no.2
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    • pp.1-6
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    • 2013
  • In this paper, we evaluate performance of existing similarity measurement metric and propose a novel method using user's preferences information entropy to reduce MAE in memory-based collaborative recommender systems. The proposed method applies a similarity of individual inclination to traditional similarity measurement methods. We experiment on various similarity metrics under different conditions, which include an amount of data and significance weighting from n/10 to n/60, to verify the proposed method. As a result, we confirm the proposed method is robust and efficient from the viewpoint of a sparse data set, applying existing various similarity measurement methods and Significance Weighting.

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In Vitro Mass Propagation and Economic Effects of Bioreactor Culture in Ever-bearing Strawberry 'Goha' (Bioreactor를 이용한 사계성 딸기 기내대량증식과 경제성)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Kwon, Young-Seok;Im, Ju-Sung;Lim, Hak-Tae;Yeoung, Young-Rok
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.845-849
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    • 2010
  • This study was conducted to compare various culture methods and evaluate economic feasibility of each method for mass propagation of new ever-bearing strawberry 'Goha'. Four different methods such as semi-solid culture, solid culture, liquid suspension culture and bioreactor culture were compared. The solid culture and bioreactor culture showed the shortest and longest root length, such as 3.6 cm and 8.3 cm, respectively. Fresh weights of plants cultured in bioreactor were 2,261 mg, which were heavier than those of cultures. Dry weights of plants cultured in bioreactor were the heavier compared to those in other cultures. The number of axillary bud developed in bioreactor was seven, but axillary bud was not developed in other cultures. Production cost through bioreactor culture was calculated to be 303 won per plant which was 542 won less than that of solid culture. As a result, we found that the bioreactor culture was the most cost effective culture method for in vitro mass propagation in new ever-bearing strawberry 'Goha'.

Effect of Preantral Follicle Isolation Technique on In Vitro Follicular Development in Mice

  • Lim, Hyun-Joo;Kim, Dong-Hoon;Im, Gi-Sun;Park, Sung-Jai;Son, Jun-Kyu;Baek, Kwang-Soo;Kwon, Eung-Gi
    • Journal of Embryo Transfer
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    • v.26 no.4
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    • pp.223-227
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    • 2011
  • The objective of this study was to compare of different isolation method of mouse preantral follicles, and to examine in vitro development of mouse preantral follicles isolated by different method. Preantral follicles were mechanically or enzymatically extracted from mouse ovaries. Mechanical isolation method used fine gauge needles and enzymatic method of isolating follicles used collagenase. The recovered preantral follicles were cultured for 10 days in alpha-minimal essential medium (${\alpha}$-MEM) + 5% FBS + Insulin-Transferrin-Selenium (ITS) + 100 mIU/ml FSH. The collected primary follicles by enzymatic treatment were higher than mechanical method. Others stage preantral follicle by mechanical isolation were higher than enzymatic method. After 10 days of culture, no statistical differences were shown in survival rates of preantral follicle among the 2 culture groups. The metaphase II rates of the oocytes were significantly higher (p<0.05) in mechanical method (17.8%) than in enzymatic method (5.1%). These results suggest that the isolation method of choice depends on the target stage preantral follicles and mechanical isolation is an optimal method of preantral folliclesin a culture of mouse preantral follicle.

A Study on Economic Analysis of Culture Program in Public Libraries (공공도서관 문화프로그램의 경제성 분석에 관한 연구)

  • Chung, Hye-Kyung;Jung, Eun-Joo
    • Journal of the Korean Society for information Management
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    • v.25 no.3
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    • pp.377-392
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    • 2008
  • The need for overall assessment of economic effectiveness of library culture program has been suggested, as its importance continues to grow. Our study proposed a new assessment method by applying DM format and IBM format to contingent valuation method that has been used for analyzing the economic value of culture program. This study also illustrates the process through case analysis so that the public library can apply this model. It is meaningful that the study provides basic framework to evaluate the fair value of the public library by minimizing the bias that the contingent valuation method has.

Microbiological Analysis of Dongchimi, Korean Watery Radish Kimchi, at the Early and Mid-phase Fermentation

  • Park, Sun-Jung;Chang, Jin-Hee;Cha, Seong-Kwan;Moon, Gi-Seong
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.892-894
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    • 2008
  • During dongchimi fermentation at 5 and $25^{\circ}C$, the pH lowered slowly and reached 4.03 at $5^{\circ}C$ after 30 days, whereas it lowered dramatically and reached 3.59 at $25^{\circ}C$ after 2 days. The predominant bacteria were Leuconostoc (Leu.) mesenteroides at $25^{\circ}C$ until day 2 which changed into Lactobacillus (Lb.) plantarum at day 3, analyzed by a culture dependent method with partial 16S rRNA gene sequencing, whereas Leu. mesenteroides occupied predominantly at $5^{\circ}C$ until day 7. In a culture-independent method using a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) with partial 16S rRNA gene sequencing, Lb. algidus was predominant at $5^{\circ}C$ until day 7 and Lb. plantarum occupied predominantly at $25^{\circ}C$ until day 3, which is different from the results of the culture based method, indicating the both methods need to be combined for accuracy. Based on the culture-dependent method, Leu. mesenteroides might be responsible for the early and mid-phase of dongchimi fermentation.

A low computational cost method for vibration analysis of rectangular plates subjected to moving sprung masses

  • Nikkhoo, Ali;Asili, Soheil;Sadigh, Shabnam;Hajirasouliha, Iman;Karegar, Hossein
    • Advances in Computational Design
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    • v.4 no.3
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    • pp.307-326
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    • 2019
  • A low computational cost semi-analytical method is developed, based on eigenfunction expansion, to study the vibration of rectangular plates subjected to a series of moving sprung masses, representing a bridge deck under multiple vehicle or train moving loads. The dynamic effects of the suspension system are taken into account by using flexible connections between the moving masses and the base structure. The accuracy of the proposed method in predicting the dynamic response of a rectangular plate subjected to a series of moving sprung masses is demonstrated compared to the conventional rigid moving mass models. It is shown that the proposed method can considerably improve the computational efficiency of the conventional methods by eliminating a large number of time-varying components in the coupled Ordinary Differential Equations (ODEs) matrices. The dynamic behaviour of the system is then investigated by performing a comprehensive parametric study on the Dynamic Amplification Factor (DAF) of the moving loads using different design parameters. The results indicate that ignoring the flexibility of the suspension system in both moving force and moving mass models may lead to substantially underestimated DAF predictions and therefore unsafe design solutions. This highlights the significance of taking into account the stiffness of the suspension system for accurate estimation of the plate maximum dynamic response in practical applications.

Effect of Improving Quality by Changing the Distribution Method of Shrimp Culture

  • KWON, Woo-Taeg;JUNG, Min-Jae;Woo, Hyun-Jin;LEE, Woo-Sik;KWON, Lee-Seung
    • Journal of Distribution Science
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    • v.19 no.4
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    • pp.53-60
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    • 2021
  • Purpose: This study focuses on exploring ways to improve the distribution method of shrimp farming so that it is eco-friendly and increases the distribution of shrimp. Research design, data and methodology: The experimental device installed in a biofloc shrimp culture in one area tested 10 times. Complex odor, concentration of H2S, water quality improvement effected by decomposition of organic substances, and degree of microbial activation measured. The data of the experimental results verified using the T-test technique, and the p value was determined based on the significance probability of 0.05. Results: This experimental device was effective in reducing odor and hydrogen sulfide in shrimp farms. With the improvement of water quality, dissolved oxygen increased due to the microbubble and cavitation action of air ejector and ultrasonic waves. In addition, the cultured microorganisms in the cultured water treated by the experimental device were remarkably proliferated compared to the raw water. Conclusions: The biofloc distribution method has a significant effect on improving water quality and reducing odor substances and will become a new eco-friendly and efficient distribution method for shrimp farming in the future.

Process Development of therapeutic antibody (ISU301) using disposable bioreactor

  • Park, Heung-Rok
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.39-39
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    • 2005
  • Large scale mammalian cell culture has become, over the past two decades, the preferred method to produce therapeutic monoclonal antibodies. In this presentation, I will introduce disposable bioreactor system and analyze key factors and points for consideration during mammalian cell culture process development. Example will be provided highlighting the selection of master cell, culture media and environmental factors based on productivity and product quality.

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