• Journal of Plant Biotechnology
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• v.46 no.3
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• pp.180-188
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• 2019

Auxin plays a crucial regulatory role in plant growth and development processes. Three major classes of auxin-responsive transcription factors controlled by the Auxin/indole-3-acetic acid (Aux/IAA), Gretchen Hagen 3 (GH3), and small auxin up RNA (SAUR) genes regulate auxin signaling. Aux/IAA, in particular, encodes short-lived nuclear proteins that accumulate rapidly in response to auxin signaling. In this study, we isolated a PagAux/IAA1 gene from poplar (Populus alba ${\times}$ P. glandulosa) and investigated its expression characteristics. The PagAux/IAA1 cDNA codes for putative 200 amino acids polypeptide containing four conserved domains and two nuclear localization signals (NLSs). Utilizing Southern blot analysis, we confirmed that a single copy of the PagAux/IAA1 gene was present in the poplar genome. The expression of this gene is specific to leaves and flowers of the poplar. PagAux/IAA1 expressed in the early exponential growth phase of cell-cultured in suspension. PagAux/IAA1 expression level reduced in drought and salt stress conditions, and the presence of plant hormones such as abscisic acid. However, expression enhanced in cold stress, cambial cell division, and presence of plant hormones such as gibberellic acid and jasmonic acid. Thus, these results suggest that PagAux/IAA1 participates in cold stress response as well as developmental processes in the poplar.

• Korean Journal of Heritage: History & Science
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• v.37
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• pp.309-326
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• 2004

There are some methods such as fatty acid analysis and microscope analysis of parasite egg and pollen and genetic analysis of ancient bacteria from ancient soil. The fatty acid analysis can examine whether some materials is human feces or animals. This is important thing to reconstruct ancient toilet culture pattern. The methods using TLC and GC-MS as organic chemistry is able to confirm ancient diet life style and nutritive conditions. The microscope analysis of ancient soil is able to confirm ancient parasite egg and pollen. It is possible to analogize ancient human diseases from this analysis. Also, genetic analysis is able to confirm genetic diversity and variation pattern of ancient organisms in archeological soil. Most of all, it is convinced of carrying through genetic preservation of exterminated ancient organisms. If archeological soils should be analysed through the natural scientific methods such as organic chemistry, soil science, microbiology, molecular biology, and genetics, this is helpful for us to understand and interpretation past historic event. And it is expected to perform an major role for understanding origin of ancient human and life style.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.56-56
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• 2019

본 연구는 식용과 약용으로 이용되는 국내 자생식물인 야산고비(Onoclea sensibilis var. interrupta Maxim.)를 기내에서 대량증식하기 위한 조건을 구명하기 위하여 수행되었다. 무가온 온실에서 수집한 포자를 기내에서 발아시켜 전엽체를 획득하였으며, 계대배양하며 실험의 재료로 사용하였다. 전엽체의 대량증식을 위해 전엽체 0.3g을 메스로 균일하게 다진 후 증류수 1ml와 함께 배지에 고루 펼쳐서 배양하는 방법을 사용하였으며, 증식에 미치는 배지의 영향을 확인하기 위하여 1/4, 1/2, 1, 2배로 조절한 MS배지를 조성하여 8주간 배양하였다. 이후, 증식이 우수한 배지를 선정하여 sucrose와 질소급원의 농도를 조절하였으며, 활성탄을 첨가하여 증식에 미치는 영향을 확인하였다. 배지종류 실험의 결과, 생체중이 1MS에서 10.2g으로 초기 접종량에 비해 가장 많이 증가하였으며, 현미경을 이용하여 관찰한 결과에서도 정상적인 전엽체의 형태인 심장형으로 발달하였다. 증식이 우수하였던 1MS배지에 sucrose의 농도를 조절하여 배양한 결과에서는 1%의 처리구에서 가장 증식률이 좋았으며, 질소급원의 경우 30mM의 농도로 조절한 처리구가 가장 좋은 결과를 보였다. 배지 내 활성탄의 첨가는 처리구당 증가된 전엽체의 생체중이 유의적인 차이를 보이지 않았다. 포자체 대량 형성을 위한 적정 배양토의 혼합조건을 확인하기 위하여 원예상토, 피트모스, 펄라이트 및 마사토의 혼합비율을 5종류로 달리하여 조성하여 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 조성한 배양토에 기내에서 배양한 전엽체 1g을 증류수와 함께 블렌더를 이용하여 10초간 분쇄하여 토양표면에 고루 분주하였다. 이후 12주간 재배한 결과, 모든 토양조합에서 포자체가 형성되었다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 포트 당 405.0개로 가장 많은 포자체가 형성되었으며, 전체적인 생육 또한 비교적 양호한 결과를 보였다. 따라서 야산 고비의 전엽체 대량증식에 적합한 배지는 경제성과 생육수준을 고려하여 1%의 sucrose와 질소급원의 농도를 30mM로 조절한 1MS 배지가 적합하며, 포자체 대량생산을 위해서는 원예상토, 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단되었다.

• The Journal of the Korea Contents Association
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• v.19 no.9
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• pp.582-592
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• 2019

This study was conducted to identify the causal pathways of variables affecting life satisfaction in adolescents with disabilities to establish a basis for concrete intervention in their daily lives. This study was conducted on 239 adolescents with disabilities aged 9 to 24 years who responded to the survey on the actual conditions of people with disability in 2017. The SPSS macro process was used for analysis of the dual mediation effect. The results showed that there was a statistically significant correlation between instrumental daily life performance capacity, life satisfaction, Self-Rated Health, and participation in culture and leisure activities. It confirmed the mediating effects of Self-Rated Health and cultural leisure activities when the instrumental daily life performance capacity affected life satisfaction. This confirmed the dual mediating effect of instrumental daily life performance capacity on life satisfaction through causal pathways of Self-Rated Health and participation in cultural leisure activities. Based on the results of this study, it was possible to identify the focus of practical intervention through the identification of sequential pathways of Self-Rated Health and cultural and leisure activities on influence of instrumental daily life performance capacity and life satisfaction in adolescents with disabilities.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.1
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• pp.12-22
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• 2019

This study derived the design aspects by carrying out the case study of Neunggok prehistoric remains park, Ansansingil historic park and Yongjuk historic park to which are taken measures to preserve undesignated cultural heritages after studying the related laws & regulations and the systems as the design conditions of historic park preserving buried cultural heritages. The results of the study are as follows. First, according to the laws & regulations related to the historical parks, the historic sites should be preserved and utilized at the same time and can have history-related facility spaces, squares, rest spaces, exercise spaces, education & culture space, and convenience spaces. Second, by the space organization and the circulation system emphasizing only the preservation of buried cultural heritages, the feature-preservation space and the functional space are separated and due to not accepting the usage behavior considering peripheral land use, the effectiveness of the historical park was low. Third, the passive feature-preservation methods such as the preservation of the exposed site in architectural methods, the reproduction of the dugout hut, and the planting Royal azaleas or displaying stone after covering up the location of the pit dwellings with soil and the usage mainly for viewing have weakened the identity of the historical park. Fourth, the fence preventing users' access interferes experiencing the features, and the vertical structure protecting the upper part of the exposed features has overwhelmed the landscape of the historical parks. Fifth, it was difficult to figure out the feature space only by the texts mainly on terminologies and the excavation photographs presented on the information signs which introduce the buried cultural heritages.

• Journal of Conservation Science
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• v.36 no.6
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• pp.578-590
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• 2020

During the 1960-1970s, a group of ceramic conservation scientists and ceramic historians in South Korea came together and established their own disciplines. While the two disciplines share the same ceramics as the subjects of their research, there has been little interaction between the two as their research outputs are articulated in remarkably different languages. This paper aims to address the following questions by using a case study that focuses on the research on white porcelains centered on the project of Gyeonggi Museum and a series of studies conducted by one of the museum's project research teams. First, what are the characteristics of and differences between the explanation styles of the two disciplines that share the same research subjects of ceramics? Second, why has the communication between the two disciplines become difficult? Third, if there can be a trading zone wherein the two disciplines would be able to communicate again, what would be its epistemic conditions? The focus of this paper is the relationship between scientific data and ordinary language, which the two disciplines have shared from their inception. By analyzing the relationship, I first argue that, as the analytical techniques of conservation science have become more developed, conservation science's data have gradually lost its relevance in ceramic history, in spite of a shared common language between them; Second, I argue that by recovering the import of shared language again, the scientific data can be placed in a different practical context, providing novel interpretations that are relevant and often consequential to ceramic history.

• Journal of Digital Convergence
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• v.19 no.4
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• pp.217-225
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• 2021

This study aimed to provide implications required to establish a content strategy by examining the influencing factors affecting the acceptance of curation services for 320 OTT users, and the main results are as follows. First, innovativeness was found to have a positive effect on performance expectations. Second, innovativeness was found to have a positive impact on the effort expectation. Third, performance expectation had a positive effect on the intention to use continuously. Fourth, it was shown that the effort expectation had a positive effect on the intention to use continuously. Fifth, social influence was found to have no significant effect on the intention to use continuously. Sixth, it was found that the facilitating conditions did not significantly affect the intention to use continuously. The above results can be assessed as the higher the OTT users perceive the performance and effort expectations of the curation service, the higher their intention to continue using them. This study is meaningful in that it verified the factors affecting the intention to use the OTT curation service and expanded the UTAUT model.

• Korean Journal of Plant Resources
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• v.32 no.4
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• pp.290-295
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• 2019

This study was carried out to select the optimal condition for prothallus propagation and sporophyte formation of Leptogramma pozoi (Lag.) Ching subsp. mollissima (Fisch. ex Kunze) Nakaike and to provide basic data for mass production system. In the propagation, 300 mg of prothallus were inoculated in different kinds of medium [1/4, 1/2, Murashige and Skoog (MS), Knop], sucrose (0, 1, 2, 3, 4%), and cultured for 8 weeks. Sporophyte formation studies were carried out by inoculating blended prothallus into artificial soils. The soils were mixed with horticultural substrate, peatmoss, perlite, and decomposed granite at different ratios or only with the horticultural substrate. Then the prothallus was cultivated for 12 weeks to figure out the formation and growth of the sporophytes. The growth and development of prothalli were excellent on MS medium. According to medium components, prothallus growth was favorable in all treatments except 0% sucrose treatment and the highest in 2% sucrose. In the experiment of soil mixtures, sporophyte formation was the highest in the horticultural substrate:perlite 2:1 (v:v) and horticultural substrate:decomposed granite 2:1 (v:v) treatment, and the overall growth was good in the horticultural substrate:decomposed granite 2:1 (v:v) mixed soil.

• Journal of Forest and Environmental Science
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• v.37 no.1
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• pp.52-61
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• 2021

Decay fungi can decompose plant debris to recycle carbon in the ecosystem. Still, they can also be fungal pathogens, which can damage living trees and/or wood material and cause a large amount of timber loss. We isolated and identified basidiomycetous fungi from the decayed bark of Mongolian oak wrapped with sticky roll traps. The degrading enzyme activities were then tested for all fungal isolates. The decay ability of selected isolates was assessed based on the weight loss of wood discs after inoculating with culture suspension of decay fungi under the different humidity levels. A total of 46 basidiomycetous fungal isolates belonged to 12 species, and 10 genera were obtained from Jong Myo (16 isolates), Chang Kyung palace (7 isolates), Cheong Gye (10 isolates), and Gun Po (13 isolates). Gymnopus luxurians was the most dominant fungus in the present study, and this species distributed in all survey sites with 9 isolates in Jong Myo, followed by 3 isolates in Chang Kyung palace, while Cheong Gye and Gun Po had only 1 isolate each. Among 46 isolates, 44 isolates secreted at least one enzyme, while 25 isolates produced both cellulase and phenol oxidase enzymes, and 2 isolates produced neither. The assessment of decay ability by artificial inoculation indicated that the weight loss of wood discs was significantly influenced by humidity conditions when inoculated with bark decay fungi. The percent weight losses by G. luxurians inoculation in RH of 90-100% and RH of 65-75% were 4.61% and 2.45%, respectively. The weight loss caused by Abortiporus biennis were 6.67% and 0.46% in RH of 90-100% and RH of 45-55%, respectively. The humidity reduction approach should be applied for further studies to control the growth and spread of bark decay fungi on the trunks wrapped with sticky roll traps.

• (The)Study of the Eastern Classic
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• no.57
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• pp.359-382
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• 2014

This paper is an essay on Chaekmun(Answer papers of official examination in Pre-modern period). Especially I tried to point out the possibility of practical use in the field of politics. For this purpose, I analyzed an examination paper written by Lee Ga-hwan, and the title is "a debate about construction of the Miang palace". Exquisite writing skill is also important in Chaekmun, but it is not everything. The subject of Chaekmun concerns various fields such like politics, economy, society, culture, and history. So that writers should have sufficient knowledge and opinion. The King, as an examiner, wants to test retainers' ability both in writing and politics. In this paper, I focused on using in field of politics between the King and the retainers. And as an good example, I analyzed a paper which dealt the event of building huge palace named Miang palace. That is because the King reflects his concerns by setting exam questions. And his concerns also could not be free of contemporary political conditions. Therefore we should be careful of reading those articles. Regarding this, Lee Ga-hwan's article had a distinctive characteristic. Unlike any other articles dealing with same event, he tried to access through creative point of view. And his thought were much close to the King's heart.