• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.560-567
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• 2008

An efficient method to produce water-soluble polysaccharides from Lentinus lepideus is described. The productivity of both endopolysaccharides (PPS) and exopolysaccharides (EPS) was compared under various culture conditions. The effect of treating their own PPS and EPS on immune cytokine production was also studied in relation to culture factors. High yield production of EPS required a moderate culture temperature $(25^{\circ}C)$ as well as long culture period (16-20 days). In contrast, PPS production required a high culture temperature $(30^{\circ}C)$ and short culture period (8 days). Most of the carbon sources did not affect polysaccharides and mycelial production except for sucrose. Immune cytokine levels in the EPS treatment varied among carbon sources or culture periods. PPS did not appear to affect much on the production of cytokines, regardless of the culturing factors, except for the culture period. These results suggest that the optimal culture conditions for L. lepideus vary according to culture purposes, and different culture conditions should be used for different targets including mycelial biomass, EPS, and PPS. Whereas the immunomodulating activitiy of EPS appeared to be affected by culture conditions in L. lepideus, that of PPS did not.

• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1115-1120
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• 2008

A novel cathepsin B inhibitor-producing bacterium was isolated from marine sediments and identified based on its 16S rDNA sequence as Pseudomonas sp. strain PB01 (Accession No. EU126129). The growth and enzyme inhibitor production were investigated under various culture conditions. A mixture of organic nitrogen source was required for the optimal production, whereas both glucose and maltose proved to be the effective carbon sources for cathepsin B inhibitor production. Other optimal culture conditions included temperature range between 25 and $28^{\circ}C$, initial medium pH of 6.6, and shaking speed of 200 rpm. Under these optimal conditions, the maximum inhibitory activity from culture broth was approximately 50% after 30 h of cultivation. Additionally, kinetic study revealed that inhibitor production paralleled with cell growth, which suggested that the inhibitor may be a primary metabolite of that bacterium.

• 한국버섯학회지
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• 제19권2호
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• pp.81-87
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• 2021

Roridin E, produced by Podostroma cornu-damae, is a mycotoxin with anticancer activity. To increase the content of roridin E, submerged culture conditions were optimized using response surface methodology. Three factors, namely, medium initial pH, incubation time and agitation speed were optimized using a Box-Behnken design. The optimum submerged culture conditions to increase the content of roridin E included a medium with an initial pH of 4.0, an incubation time of 12.90 days, and an agitation speed of 63.03 rpm. The roridin E content in the submerged culture, under the aforementioned conditions, was 40.26 mg/L. The findings of this study can help lower the current price of roridin E and promote its related research.

• 식물조직배양학회지
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• 제26권4호
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• pp.223-227
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• 1999

본 연구는 글라디올러스 캘러스로부터의 재분화 조건을 구명함으로써 캘러스 배양시스템을 확립하는데 기여하고자 하였다. 글라디올러스 캘러스로부터의 유식물체 획득은 캘러스를 2,4-D 무첨가 1/2 MS고체배지에 치상한 후 24시간 일장하에서 15$^{\circ}C$로 배양하였을 때 효과적이었다. 캘러스로부터의 기관형성에 미치는 배양방법의 영향을 검토한 결과, 부정근의 형성은 액체진탕배양이 그리고 부정아의 형성은 액채정치배양이 각각 우수한 것으로 확인되었다. 본 실험의 결과를 통해 글라디올러스 ‘Topaz' 캘러스로부터의 재분화를 위한 최적 배양 조건을 선발하는 한편, 액체진탕배양법에 의해 기관-캘러스 혼합체를 유도할 수 있었으며, 이를 이용하여 기내 유식물체를 대량생산할 수 있는 가능성을 확인할 수 있었다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권1호
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• pp.6-10
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• 2001

The production of chitosan from the mycelia of Absidia coerulea was studied to improve cell growth and chitosan productivity. Culture conditions were optimized in batch cultivation (pH 4.5, agitator speed of 250 rpm, and aeration rate of 2 vvm) and the maximum chitosan concentration achieved was 2.3g/L under optimized conditions. Continuous culture was carried out successfully by the formation of new growth spots under optimized conditions, with a chitosan productivity of 0.052g/L(sup)-1 h(sup)-1, which is the highest value to date, and was obtained at a dulution rate of 0.05h(sup)-1. Cell chitosan concentrations reached about 14% in the steady state, which is similar to that achieved in batch culture. This study shows that for the continuous culture of Absidia coerulea it is vital to control the medium composition.

• 약학회지
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• 제36권4호
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• pp.390-396
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• 1992

Streptomyces minoensis DMCJ-144 isolated from soil produces the ${\alpha}-amylase$ inhibitor. Optimum culture conditions for ${\alpha}-amylase$ inhibitor production of the strain were determined in this experiment. The optimum composition of the culture medium was studied by supplementing various carbon sources, nitrogen sources, vitamins, and metal salts to the basal medium containing 1% glucose, 0.1% asparagine, 0.005% $MgSO_4{\cdot}7H_2O$, 0.005% $K_2HPO_4$, 0.005% NaCl. Other culture conditions such as the culture temperature, initial pH of the medium, aeration, and culture time were also investigated. When the strain was cultured in a 100 ml flask containing 20 ml of 2% glucose, 0.5% beef extract, 0.0002% riboflavin, 0.0002% thiamine HCI, 0.01% $ZnCl_2$, 0.005% $MgSO_4{\cdot}7H_2O$, 0.005% $CuSO_4{\cdot}5H_2O$, 0.005% NaCl, pH 7.2, 180 rpm at $30^{\circ}C$, the maximum production of the ${\alpha}-amylase$ inhibitor was observed after 5 days of the cultivation.

• 한국동물생명공학회지
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• 제39권1호
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• pp.19-30
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• 2024

Background: Although an understanding of the proliferation and differentiation of fish female germline stem cells (GSCs) is very important, an appropriate threedimensional (3D) research model to study them is not well established. As a part of the development of stable 3D culture system for fish female GSCs, we conducted this study to establish a 3D aggregate culture system of ovarian cells in marine medaka, Oryzias dancena. Methods: Ovarian cells were separated by Percoll density gradient centrifugation and two different cell populations were cultured in suspension to form ovarian cell aggregates to find suitable cell populations for its formation. Ovarian cell aggregates formed from different cell populations were evaluated by histology and gene expression analyses. To evaluate the media supplements, ovarian cell aggregate culture was performed under different media conditions, and the morphology, viability, size, gene expression, histology, and E2 secretion of ovarian cell aggregates were analyzed. Results: Ovarian cell aggregates were able to be formed well under specific culture conditions that used ultra-low attachment 96 well plate, complete mESM2, and the cell populations from top to 50% layers after separation of ovarian cells. Moreover, they were able to maintain minimal ovarian function such as germ cell maintenance and E2 synthesis for a short period. Conclusions: We established basic conditions for the culture of O. dancena ovarian cell aggregates. Additional efforts will be required to further optimize the culture conditions so that the ovarian cell aggregates can retain the improved ovarian functions for a longer period of time.

• 한국농공학회:학술대회논문집
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• 한국농공학회 1998년도 학술발표회 발표논문집
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• pp.224-229
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• 1998

This study was conducted to develop the simple and convenient irrigation control model which can maintain the appropriate rates of irrigation and drainage of nutrient solution according to the environmental conditions and growth stages in soilless culture of cucumber. In order to obtain fundamental data for development of the model, investigation of the actual state of soilless culture practices was carried out. Most irrigation systems of soilless culture were controlled by the time clock. Evapotranspiration of cucumber in soilless culture was investigated and correlations with environmental conditions were analyzed, and its prediction model was developed. A irrigation control model based on the time clock control and there were considered seasons, weather conditions, and growth stages was developed. Applicability of the model was tested by simulation. Drainage rates of irrigation system controlled by conventional time clock, integrated solar radiation, and the developed model were 61%, 20%, and 32%, respectively in cucumber perlite culture.

• 한국농공학회지
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• 제41권2호
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• pp.37-43
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• 1999

This study was conducted to develop the simple and convenient irrigation control model which can maintain the appropriate rates of irrigation and drainage of nutrient solution according to the enviornmental conditions and growth stages in soilless culture of cucumber. In order to obtain fundamental data for development of the model, investigation of the actual state of soilless culture practices was carried out. Most irrigatioin systems of soillness culture were controlled by the time colock. Evapotranspiration of cucumber in soilness culture was investigated and correlations with environmental conditions were analyzed , and its estimating model was developed. In order to develop the irrigation system which can control the amount of nutrient solution applied according to seasons, weather conditions, and growth stages, a irrigation clock control was developed. Applicability of the model was tested by simulation. Drainage rates of nutrient solution controlled by conventional time clock, integrated solar radiation, and the developed model were 61% , 20%, and 32% , respectively in cucumber perlite culture.

• 한국컴퓨터정보학회논문지
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• 제10권5호
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• pp.301-312
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• 2005

본 논문에서는 문화산업이 국가경쟁력의 새로운 척도로 부각되는 가운데 국가단위에서 지역도시단위로 옮아가고 있고 지역 특성화 방안으로 문화산업 즉, 엔터테인먼트산업에 대한 지역 및 주민의 요구가 크고 다양하게 증대되고 있다. 문화산업의 도시별 차이점을 고찰함으로서 대 . 중소도시별 상관관계와 문화산업의 접근방법에 따른 이해와 이용의 차이를 분석하였고 대도시와 중소도시에 따른 변화나 문화정책도 연구목적으로 하였다. 연구결과에서 첫째, 문화가 변화함에 따라 문화산업이 변화하는 것으로 나타났다. 둘째, 사회적 여건에 따라 도시별 차이점을 발견할 수 있었는데 대도시에선 대중문화 부상이 영향을 미치는 요인으로 나타났으며 중소도시에서는 대중문화 부상이 영향을 미치고 있으나 문화생활화도 중요시됨을 도출 할 수 있었다. 셋째, 경제적인 여건에 따라 도시별 차이점을 발견할 수 있었는데 대도시에선 문화유통체계다양화와 문화상품화, 문화클러스터화가 고루 영향을 미치는 요인으로 나타났으며 중소도시에서는 문화유통체계다양화와 문화상품화가 영향을 미치고 있으나 문화 클러스터화에 대해 영향을 못 미치는 것으로 나타났다.