• Title/Summary/Keyword: cultural technology

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Isolation and Identification of Lactic acid Producing Bacteria from Kimchi and Their Fermentation Properties of Soymilk (젖산 생성능이 우수한 김치 유래 젖산균의 분리 및 두유 발효 특성)

  • Lee, Lan-Sook;Jung, Kyung Hee;Choi, Ung-Kyu;Cho, Chang-Won;Kim, Kyung-Im;Kim, Young-Chan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.11
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    • pp.1872-1877
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    • 2013
  • Lactic acid bacteria were selected on the basis of lactic acid producing ability from kimchi, a traditional Korean fermented food. Among the initial screening of over 150 strains selected from the sample, 27 strains were selected as lactic acid producing bacteria, and 4 strains were finally selected based on their ability to produce relatively high levels of lactic acid. The four strains were identified as Lactobacillus (L.) plantarum Gk04, Pediococcus pentosaceus Gk07, L. brevis Gk35 and L. curvatus Gk36 by the conventional morphological, cultural, physiological and biochemical characteristics, as well as by 16S rRNA sequence analysis. Among the identified lactic acid bacteria, L. curvatus Gk36 was used for soymilk fermentation. The viable cell counts and acidity values measured for the L. curvatus Gk36 were comparable to the commmercial L. acidopillus. Thus, the L. curvatus Gk36 is a potential probiotic strain to prepare fermented soy products, such as kephir, yogurt, tempeh and soy sauce.

A Comparison of Growth and Reproduction of Lumbricus rubellus and Eisenia foetida Cultured in Three Kinds of Artificial Soil Substrates (몇가지 인공토양에서 사육한 Lumbricus rubellus와 Eisenia foetida의 성장인자의 차이)

  • Lee, Sung-Kyu
    • Korean Journal of Environmental Agriculture
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    • v.14 no.1
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    • pp.82-87
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    • 1995
  • The standardization of test procedures and reproducibility of the toxicity data are prerequisite for the toxicity testing with the earthworm culturing in the laboratory. No in-depth study on culturing conditions of earthworms has been conducted in Korea, even of massive cultural practice is common for composting and production of biochemicals. The earthworms, Lumbricus rubellus and Eisenia foetida, were cultured in three kinds of artificial soil substrates(I, II and III) based on the OECD Guideline, which consist of different ratios of components (sand, sphagnum peat and kaolinite), and fed with a mixture of grain powders. During the period of culturing, the body weight and reproduction parameters were measured. L. rubellus showed the best results for increasing body weight and cocoon production in the artificial soil substrate(I) compared with E. foetida. The cocoon production was significantly high in both species cultured in the artificial soil substrate(I) among the three kinds of soil substrates, but the cumulative cocoon production of L. rubellus was 11 cocoon per worm compared with 3.7 cocoons per worm of E.foetida. L. rubellus, therefore, was more prolific than E. foetida in these culture schemes. The cumulative mortality in both species was less than 10%, and the number of juvenile worms per cocoon ranged from 1.5 to 2.3 and thus did not show any relationships with soil substrates or species. From these data, the culture of L. rubellus in the laboratory could be standardized, but for E. foetida, further study would be necessary to establish the optimal growth conditions in the laboratory.

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LIS Employment Prospects: LIS Students' Perspective (문헌정보학과 재학생의 취업전망에 대한 인식조사 연구)

  • Noh, Younghee;Ahn, In-Ja;Lee, Jongmoon;Oh, Se-Hoon
    • Journal of the Korean Society for Library and Information Science
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    • v.47 no.4
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    • pp.143-167
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    • 2013
  • The power of human capital in a library is the main resource that determines the level of library services, which in turn should play a pivotal role in forming the intellectual power of the country. This research conducted a survey on the employment perspectives of LIS undergraduates, which resulted as follows. First, among the LIS undergraduates, 40% of students responded that their desire was to be employed in the library as librarians. Although there were some negative opinions regarding the working environment of the industry, the undergraduates appreciated the developmental possibilities. Second, the most desired task was working with cultural programs. Third, the respondents were satisfied with the current curriculum overall, acknowledging the extra need for job search support programs, which include career support, employment-related mentoring, and internships. Fourth, the main requirements for their future employability were perceived as grade point average, language skills, obtaining licenses, internship and apprenticeship experience, and career management. Fifth, influential factors on employment decision making were divided into two aspects: environmental and personal factors. The environmental factors included salary, welfare, opportunities in personal development, workplace environment, organizational culture, and development possibilities of the organization. The personal factors included fitness with personal aptitude, the level of knowledge and technology in the job industry, social norms and acknowledgement, rewarding opportunities with their major, and location of job.

A Study on Deterioration of Stone Monuments by Acid Fog (산성안개에 의한 석조문화재 구성암석의 손상 연구)

  • Do, Jin Young;Kim, Sang Woo;Cho, Hyen Goo
    • Journal of the Mineralogical Society of Korea
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    • v.28 no.2
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    • pp.135-145
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    • 2015
  • In order to predict the deterioration of stone monument due to acid fog, an artificial fog test using pH4.0 and pH5.6 was applied to the Gyeongju Namsan granite, decite and marble. After the test had weathered Gyeongju Namsan granite a larger weight reduction due to acid fog than fresh one. Decite has shown the most significant changes among the tested rocks with about 0.005 % of weight reduction. Decite and weathered granite will have considerable weight reduction due to acid rain than the acid fog, whereas the marble was expected to show a weight reduction regardless of the phase of water. The porosity and water absorption rate of weathered granite had significantly increased. This result means that the weathered rock is predicted to be more susceptible to acid fog than the fresh rock. The absorption rate of the marble after the test had shown approximately 50 % increase. The color of the samples had slightly changed towards yellow, such tendency was greater shown in weathered rocks. The marble reacted with acid fog had an increased whiteness. A large amount of cation in the samples is caused mainly by the dissociation of minerals through the reaction with acid fog.

Isolation and Identification of Entomopathogenic Bacteria for Biological Control of the Mushroom Fly, Lycoriella mali (느타리 재배에서 버섯파리의 생물학적 방제를 위한 곤충병원성 세균의 분리 및 동정)

  • Lee, Su-Hee;Lim, Eun-Kyung;Choi, Kwang-Ho;Lee, Jae-Pil;Lee, Hyun-Ouk;Kim, Ik-Soo;Moon, Byung-Ju
    • The Korean Journal of Mycology
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    • v.30 no.1
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    • pp.44-49
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    • 2002
  • The study was conducted to isolate and identify insecticidal bacteria for biological control of larvae of mushroom fly, Lycoriella mali, which is one of serious pests to oyster mushrooms during its cultivation period. Among eight bacteria isolated from the soil in the oyster mushroom beds and the dead body of L. mali, two bacteria, Bti-D and Bti-U showed more toxicity with mortality rate than other six-bacteria isolates. The two bacteria showed more toxicity in three instar of the period of development of the mushroom fly than in other instar. Symptoms of the larvae of L. mali infected by the two bacteria developed as follows: at the early infection, the front middle gut changed color to light brown, the middle gut to brown, whole body to black brown, and eventually, the fly died. For the identification of these isolates, cultural and biochemical characteristics by Bergey's manual and Biolog system, cell morphology by TEM, endospore and endotoxin by phase-contrast microscope, and test using 33H antisera were examined. According to the results, these two isolates, Bti-D and Bti-U were identified as Bacillus thuringiensis subsp. israelensis respectively.

Improvement of cultural efficiency using DNA markers in anther and seed culture of rice (DNA marker를 이용한 벼의 조직배양 효율개선)

  • Kim, Hong-Jib;Kim, Tae-Heon;Sohn, Jae-Keun
    • Current Research on Agriculture and Life Sciences
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    • v.27
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    • pp.21-28
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    • 2009
  • The purpose of this study was to improve the culturability of 'IR 36', a indica type rice cultivar using DNA marker associated with the ability of plant regeneration in anther and seed culture. The culturability of 6 rice cultivars and 2 indica/japonica lines ('MGRI 036', 'MGRI 079') were investigated in anther and seed culture. The culturability of 3 japonica rice cultivars were much higher than tongil and indica rice cultivars, and 'MGRI 036' and 'MGRI 079' has high culturability with 20% regenerability, also. 34 $BC_2F_4$ 4 lines were selected by marker screening using RZ400 among 90 $BC_2F_4$ lines derived from a cross between 'MGRI 079' and 'IR 36'. The frequency of callus formation of 10 $BC_2F_4$ lines were higher than 'IR 36' in anther culture among selected 34 $BC_2F_4$ lines. The ability of plant regeneration of 10 lines were higher than 'IR 36' in the seed culture among selected 34 $BC_2F_4$ lines. A promising line, $BC_2F_4$-28, was selected to have better culturability in the anther and seed culture among selected 34 $BC_2F_4$ lines. The heading date and grain shape of the $BC_2F_4$-28 was similar to 'IR 36'. Using the RZ400 DNA marker associated with the culturability will be useful method for improving of indica rice culticvar's culturability in rice breeding program.

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Variation of Glucosinolate Contents among Domestic Broccoli (Brassica oleracea L. var. italica) Accessions (국내 브로콜리(Brassica oleracea L. var. italica) 유전자원 내 Glucosinolate 함량 변이)

  • Lee, Jun Gu;Kwak, Jung-Ho;Um, Yeong Cheol;Lee, Sang Gyu;Jang, Yoon-Ah;Choi, Chang Sun
    • Horticultural Science & Technology
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    • v.30 no.6
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    • pp.743-750
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    • 2012
  • A total of 95 broccoli (Brassica oleracea L. var. italica) accessions were evaluated for the identification of desulfo-glucosinolates and their content variation in the flower head using ultra performance liquid chromatography, to select the potentially functional broccoli breeding lines. The six individual desulfo-glucosinolates, including progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicanapin, and glucobrassicin, were commonly identified, based on the chromatogram peak comparison with those of the nine individual glucosinolate standards. The total glucosinolate contents varied from 4.2 to $29.0{\mu}mol{\cdot}g^{-1}$ DW and the glucoraphanin (1.6 to $13.9{\mu}mol{\cdot}g^{-1}$ DW) was confirmed as a major constituent in the total glucosinolate profile among the six identified individual glucosinolate species, whereas the progoitrin, which was only detected in 13 accessions, showed accession-specific variation and negative correlation with glucoraphanin content. It was also revealed that the four major glucosinolates, such as glucobrassicanapin, glucoraphanin, glucobrassicin, and gluconapin, affected major content variation and showed higher positive inter-correlation. These results might be used for the selection of potential breeding materials as functional broccoli germplasm through the further evaluation on the stability and reproducibility of glucosinolate profile depending on environmental factors or cultural managements using the selected accessions.

Study of Lime Finishing Layer(intonaco) in Koguryo Tomb Murals (고구려 고분벽화의 석회마감층에 관한 연구)

  • Lee, Hwa-Soo;Han, Kyeong-Soon
    • Journal of Conservation Science
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    • v.19
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    • pp.43-56
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    • 2006
  • We checked the conditions and material properties after producing the lime finishing layers with a mortar mix of processed lime. In addition, we reproduced the lime finishing layers of the Koguryo Tomb Murals, colored the layers with a few different techniques and identified the characteristics for each painting technique. As a result of the experiment, we could infer the producing techniques as well as teaming the conditions and properties of the lime finishing layers of the Koguryo tomb murals, and examined the maintenance of the lime finishing layers. Of those samples produced under various conditions, the layers of slaked lime, which were obtained after being hydrated underground for a long time, showed quite a good condition. Thus the slaked lime mixes produced through particular hydration methods are judged to increase the maintenance of the lime finishing layers of the Koguryo Tomb Murals. Mostly, the paints of Koguryo Tomb Murals indicated that they have similar conditions with the samples produced with a secco method. In addition, Considering the compositional forms of the painting layers and the boundary layers which formed between the painting layers and the finishing layers, we can verify that, in some cases, the conditions of the painting layers of the Koguryo tomb murals are similar to those which were produced with a combination of fresco and secco.

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Advertisement Criticism through Audience Response and Communication Efficacy - focused on KT&G TV-CM text - (수용자 반응 중심의 광고비평과 커뮤니케이션 실효성 - KT&G TV광고 텍스트를 중심으로 -)

  • Lee, Hyun-Woo
    • Archives of design research
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    • v.19 no.5 s.67
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    • pp.233-242
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    • 2006
  • The purpose of this study is to investigate communication efficacy of advertisement text through audience responses. This study approached qualitatively to KT&G TV commercials. The research proceeded as follows: First, as background theory, studies on audience focusing advertisement criticism and the interpretation of advertising texts were reviewed. Secondly, the characteristics of the audiences of the advertisements, analyzed through in-depth individual interviews as well as group interviews, were incorporated into a broad theme and then divided into different dimensions. Finally, the audiences' decoding code and critic reponses in reading ambiguous advertising texts, and the interrelationship between strategic ambiguity were discussed under a unified model. The major findings of this study are as follows: In interpreting the ambiguous advertising texts, the audiences use various decoding codes such as language, visuals, technology and rhetoric, and various critic responses such as linguistic, macroscopic, schematic, non-verbal and socio-cultural factors, in quite comprehensive manner. Also, it was shown that audiences make use of different decoding strategies in terms of their recognition, reliability, emotional attitude, and behavior. It can therefore be concluded that the strategic ambiguity has its limit in explaining its effectiveness in the entire dimensions of recognition, emotional attitude, and behavior, in the sense that the strategic ambiguity is most effective in recognition while it invokes more negativity in the behavioral dimension. Finally, this empirical study, focusing on qualitative analyses, may have its limit as well; however, deeper statistic-qualitative studies in the future could compensate for it.

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A Study on the Convergence of Digital and Analog Art -Art Collaboration Focused on My Series- (예술에 있어서 디지털과 아날로그 융합에 관한 연구 -아트콜라보레이션 본인작품 <결합체>시리즈를 중심으로-)

  • Ryu, Ji Eun;Yang, Jong Hoon
    • The Journal of the Korea Contents Association
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    • v.17 no.4
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    • pp.510-519
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    • 2017
  • Our world is communicating by digital network that transcends time and space. In such world of rich technology, people are longing for humanity and analog esthetic. 'Art' stimulates analog esthetic and 'design' is a good and easy tool to show personal esthetic preferences. This study is about the convergence of digital and analog art focusing on the researcher's artwork series. The series is derived from series. They are made by cut and deconstructed pieces from the original series. I assembled these pieces into another creation by using graphic editing programs. I looked into the succession cases of art and design collaborations and developed various design products with the assembled artwork images. Through this, I want to communicate more familiarly with the world widely connected to network viewers. There are already many fine artists making their artworks into design products. Collaboration with cultural art and commercial product provides win-win effect to artist, company, and consumer. It also creates innovative products and values. In this study, I try to design products from various fields using my artwork images, and research about how to promote the artist and art products effectively to the world.