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http://dx.doi.org/10.4489/KJM.2002.30.1.044

Isolation and Identification of Entomopathogenic Bacteria for Biological Control of the Mushroom Fly, Lycoriella mali  

Lee, Su-Hee (Faculty of Natural Resources and Life Science, Dong-A University)
Lim, Eun-Kyung (Faculty of Natural Resources and Life Science, Dong-A University)
Choi, Kwang-Ho (Faculty of Natural Resources and Life Science, Dong-A University)
Lee, Jae-Pil (Faculty of Natural Resources and Life Science, Dong-A University)
Lee, Hyun-Ouk (Faculty of Natural Resources and Life Science, Dong-A University)
Kim, Ik-Soo (Sericulture & Entomology, National institute of Agricultural Science and Technology)
Moon, Byung-Ju (Faculty of Natural Resources and Life Science, Dong-A University)
Publication Information
The Korean Journal of Mycology / v.30, no.1, 2002 , pp. 44-49 More about this Journal
Abstract
The study was conducted to isolate and identify insecticidal bacteria for biological control of larvae of mushroom fly, Lycoriella mali, which is one of serious pests to oyster mushrooms during its cultivation period. Among eight bacteria isolated from the soil in the oyster mushroom beds and the dead body of L. mali, two bacteria, Bti-D and Bti-U showed more toxicity with mortality rate than other six-bacteria isolates. The two bacteria showed more toxicity in three instar of the period of development of the mushroom fly than in other instar. Symptoms of the larvae of L. mali infected by the two bacteria developed as follows: at the early infection, the front middle gut changed color to light brown, the middle gut to brown, whole body to black brown, and eventually, the fly died. For the identification of these isolates, cultural and biochemical characteristics by Bergey's manual and Biolog system, cell morphology by TEM, endospore and endotoxin by phase-contrast microscope, and test using 33H antisera were examined. According to the results, these two isolates, Bti-D and Bti-U were identified as Bacillus thuringiensis subsp. israelensis respectively.
Keywords
Bacillus thuringiensis subsp.; israelensis; Identification; Lycoriella mali; Oyster mushrooms;
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