• Title/Summary/Keyword: cultivation activities

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Phenolic Compounds Content and DPPH, ADH, ALDH Activities of Mungbean Sprout Based on Growth Temperature (녹두나물 재배온도에 따른 페놀화합물 함량과 DPPH, ADH 및 ALDH 활성)

  • Kim, Dong-Kwan;Son, Dong-Mo;Chon, Sang-Uk;Lee, Kyung-Dong;Kim, Kyong-Ho;Rim, Yo-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.1
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    • pp.1-6
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    • 2009
  • This study is to analyze the effects of the growth temperature of mungbean sprouts ($15{\sim}30{\pm}1^{\circ}C$) on the yield ratio, content of phenolic compounds and DPPH (1,1-diphenyl-2-picrylhydrazyl), ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase) activities of the sprouts. When the growth temperature of mungbean sprouts was higher, the yield ratio of the sprouts was higher while the hard seed rate was lower, but $25{\pm}1^{\circ}C$ and $30{\pm}1^{\circ}C$ showed no regular tendency. The content of the total phenol from the ethanol extract of the sprouts was higher in the growth temperature of $15{\pm}1$, $20{\pm}1$, and $25{\pm}1^{\circ}C$, while the content of total flavonoid was higher in the growth temperature of $15{\pm}1$, and $20{\pm}1^{\circ}C$. The DPPH radical scavenging activity of the ethanol extract of the sprouts was higher when the growth temperature was lower, while the activity of ADH and ALDH showed no regular tendency according to the growth temperature. Considering the yield ratio, content of phenolic compounds, biological activities of mungbean sprouts, the optimum cultivation temperature of mungbean sprouts may be $20{\sim}25^{\circ}C$.

Chemical properties and antioxidant activities of the sprouts of Kalopanax pictus, Cedrela sinensis, Acanthopanax cortex at different plucking times (채취시기별 엄나무, 참죽, 오가피 햇순의 화학적 특성 및 항산화 활성)

  • Im, Hee-Jin;Jang, Hye-Lim;Jeong, Yong-Jin;Yoon, Kyung-Young
    • Food Science and Preservation
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    • v.20 no.3
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    • pp.356-364
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    • 2013
  • The nutritional composition, bioactive components and antioxidant activities of the first and second sprouts of Kalopanax pictus, Cedrela sinensis and Acanthopanax cortex were investigated to increase the utilization of these sprouts. The moisture and crude lipid contents of the first sprout were higher than those of the second sprout, and the crude fiber and carbohydrate contents were higher in the second sprout. The organic acid content of the first sprout was higher than that of the second sprout. The second sprout had higher free amino acid contents in K. pictus and C. sinensis, and the first sprout had a higher content in A. cortex. Especially, the second sprout of K. pictus and C. sinensis had a higher level of glutamic acid, which is a major taste component of foods, than the first sprout. The second sprouts of all the samples had higher mineral levels than the first sprouts. In contrast, the first sprouts had higher total polyphenol and flavonoid contents and showed a higher antioxidant activity level, except for the DPPH radical scavenging activity of the A. cortex. The results of this study show that the nutritive value of the second sprout was higher than that of the first sprout, but the bioactive components value of the second sprout was lower than that of the first sprout. Therefore, additional research is needed on the cultivation condition required to maintain the nutritive values and the antioxidant activities of these sprouts.

Elevation of anti-oxidative activity and inhibitory activities against tyrosinase, elastase, collagenase and hyaluronidase of Oplismenus undulatifolius by elicitor treatment (Elicitor 처리한 주름조개풀(Oplismenus undulatifolius)의 항산화, tyrosinase, elastase, collagenase 및 hyaluronidase 억제 활성 증대)

  • Lee, Eun-Ho;Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • v.63 no.3
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    • pp.221-227
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    • 2020
  • This study was designed to assess the change of the antioxidative and biological enzyme activities [tyrosinase, elastase, collagenase, and hyaluronidase (HAase)] of extracts from elicitor-treated Oplismenus undulatifolius. Elicitor-treated O. undulatifolius showed higher total phenolic content than the non-treated extract. As a result of comparing the anti-oxidant activity of elicitor-treated O. undulatifolius extract and non-treated extract, the elicitor-treated group showed high activity. Elicitor-treated O. undulatifolius showed higher elastase and collagenase inhibitory activities as anti-wrinkle effect, tyrosinase inhibitory activity as whitening effect, and anti-inflammation effect to induced as HAase inhibition than the non-treated extract. Therefore, elicitor-treatment during O. undulatifolius cultivation in outdoors will elevate total phenolics content in the plant and elevate of various bioactivities, which will yield high quality for industrialization.

Evaluation of the In vitro Activity of Glycyrrhiza Cultivar Roots (감초 육성품종 뿌리 추출물의 In vitro 활성 평가)

  • Lee, Seung Eun;Lee, Jeong Hoon;Park, Chun Geon;Kim, Hyung Don;Lee, Yun Ji;Seo, Kyung Hae;Jeong, Hyeon Soo;Chang, Jae Ki;Kim, Dong Hwi
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.2
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    • pp.115-125
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    • 2019
  • Background: Glycyrrhiza radix (licorice root) have been used as an oriental medicine material for long time, and its protective effects on oxidative stress, inflammation and cognition deficit have been recently reported. However, the cultivation of Glycyrrhiza species as medicinal crops is associated with some problems such as low productivity and early leaf fall, etc. To resolve this problems, Glycyrrhiza cultivars have been developed by direct hybridization of each Glycyrrhiza species by Korean researchers. The present study was conducted to compare the Glycyrrhiza cultivar radix (Dagam, Sinwongam and Wongam) for their anti-oxidation, anti-inflammation, and cognition improvement effects and levels of liquiritin, isoliquiritigenin and licochalcone in order to select an excellent cultivar as a material resource. Methods and Results: For evaluating the inhibitory efficacies of the Glycyrrhiza cultivar extracts on oxidative stress and inflammation in BV2 cells, we measured their reactive oxygen species (ROS) production and nitric oxide (NO) release after treating them with lipopolysccharide. The scavenging activities on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and peroxynitrite ($NOO^-$) radicals were evaluated. Cell proliferation and N-methyl-D-aspartate receptor (NMDAR) inhibition were analyzed. The total phenol, liquiritin, isoliquiritigenin and licochalcone A content in the extracts of the three culivars were quantified. Furthermore, the correlation coefficient between the activities and contents of total phenol, liquiritin, isoliquiritigenin and licochalcone A were also calculated. The results indicated that Sinwongam exhibited potent anti-oxidant, anti-inflammatory and NMDAR inhibititory activities. Sinwongam also showed higher total phenol and licochalcone A contents than the other cultivars. Among the three cultivars, Dagam exhibited a positive effects on NO release inhibition, cell proliferation and contents of liquiritin and isoliquiritigenin. Conclusions: Sinwongam is expected to be the most useful resource as a functional material for anti-oxidation/anti-inflammation and cognition improvement among the three studied licorice cultivars.

Functional Bioactive Compounds and Biological Activities of Vaccinium oldhamii (정금나무의 기능성 생리활성 물질과 생리활성)

  • Chae, Jung-Woo;Jo, Huiseon
    • Journal of Life Science
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    • v.32 no.2
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    • pp.167-174
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    • 2022
  • In modern society, interest in antioxidants is increasing as the stress caused by oxidants increases. However, the demand for synthetic antioxidants is decreasing because some studies have confirmed that they are harmful when consumed in large quantities; thus, studies on antioxidants derived from natural substances are actively being conducted to replace synthetic antioxidants. Blueberry, known as one of the world's top ten long-lived foods, is a plant of the Vaccinium (Ericaceae) family, and various pharmacological activities of blueberry including antioxidant activity have been studied. Vaccinium oldhamii (VO) is a deciduous broad-leaved shrub in the same genus as blueberries, and in this paper, we summarize the studies on the efficacy analysis of VO extracts and purified products. The content of phenolic compounds in VO fruits was proportional to antioxidant and anti-influenza activity such as the inhibition of NO production, and the total content of polyphenols and anthocyanin was higher than that in blueberries. VO fruit extracts showed anti-inflammatory activity and anti-cancer activity against human acute leukemia; in contrast, VO branch extracts showed anti-inflammatory activity, activity to inhibit osteoclast differentiation and bone resorption due to inflammatory response, and anti-cancer activity against several human cancer cell lines. Compared to blueberries, VO showed higher phenolic compound content, antioxidant activity, and various physiological activities. In addition, VO is considered to have sufficient value as an alternative crop to blueberries, such as it can be grown natively in Korea, with simple mass cultivation and no need to pay royalties for commercialization.

Antioxidant Compounds and Antioxidant Activities of Sweet Potatoes with Cultivated Conditions (재배조건에 따른 고구마의 항산화성분 및 항산화활성)

  • Woo, Koan-Sik;Seo, Hye-In;Lee, Yong-Hwan;Kim, Hyun-Young;Ko, Jee-Yeon;Song, Seuk-Bo;Lee, Jae-Saeng;Jung, Ki-Yuol;Nam, Min-Hee;Oh, In-Seok;Jeong, Heon-Sang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.4
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    • pp.519-525
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    • 2012
  • Effects of cultivated conditions on antioxidant compounds and antioxidant activities of sweet potatoes (Ipomoea batatas (L.) Lam) were determined. The cultivated variety was Shinyulmi, and they were cultivated in a conventional culture, successful cropped hairy vetch culture, successful cropped barley cultivation, successful cropped rye cultivation, successful cropped mix-seeding of hairy vetch and barley, successful cropped mix-seeding of hairy vetch and rye, and not fertilizer. The brix degree, moisture, protein, and ash content of the sweet potatoes did not significantly change with the cultivated conditions. However amylose, total dietary fiber, and mineral content had significant changes. The total polyphenol, flavonoid, and tannin content of the methanolic extracts of the sweet potato's pericarp showed significant differences from cultivated conditions, however, the sweet potato's sarcocarp did not significantly change. The highest DPPH and ABTS radical scavenging activities of the methanolic extracts of the sweet potatoes were 958.81 and 663.53 mg TE/100 g in the sweet potato's pericarp on the successful cropped hairy vetch culture. Generally, there was a difference in antioxidant compound content and radical scavenging activity on the methanolic extract of sweet potato with cultivated conditions.

Biocontrol of Red Pepper Using Mixed Culture of Antagonistic Bacterium and Phosphate Solubilizing Yeast (항진균 세균과 난용성 인산염 가용화 효모의 혼합 배양액을 이용한 고추 병해의 생물학적 방제)

  • Lee, Gun Woong;Min, Byung-Dae;Park, Sujeong;Jheong, Weonhwa;Go, Eun Byeul;Lee, Kui-Jae;Chae, Jong-Chan
    • Korean Journal of Microbiology
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    • v.49 no.4
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    • pp.398-402
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    • 2013
  • This study was to investigate beneficial effects of microbial mixture on red pepper which was capable of promoting plant growth by solubilizing insoluble phosphate as well as protecting plants from pathogenic attack. Saccharomyces sp. L13 was isolated for phosphate solubilizing activity on aluminium phosphate, tricalcium phosphate, calcium hydrophosphate, and magnesium hydrophosphate. On the other hand, Bacillus sp. L32 was isolated for antagonistic activity against Phytophthora capsisi and Colletotrichum gloeosporioides, causing Phytophthora blight and Anthracnose disease in pepper, respectively. The strain L32 exhibited antagonistic activities both under dual culture assays and detached leaves assays. The each strain under the condition of mixed cultivation exhibited the same growth rates as one under pure cultivation. In greenhouse study, the mixed culture showed the both effect of plant growth promotion and reduction of disease symptom development against P. capsisi and C. gloeosporioides providing a potential as effective microbial agent for plant husbandry.

Occurrence of Diseases and Case of Clinical Diagnosis on Watermelon in South Korea, 2008-2012

  • Noh, Jaejong;Kim, Ju-Hee;Lim, Jeong Hyeon;Kim, Tae Bok;Seong, Mun Ho;Jung, Gi Tai;Kim, Jeong Man;Cheong, Seong-Soo;Oh, Nam Ki;Lee, Wang-Hyu
    • Research in Plant Disease
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    • v.20 no.1
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    • pp.8-14
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    • 2014
  • The socio-network activities for regional governments, agricultural research institutes, developing agencies and policy makers of any countries are very crucial and important to take into account the root cause of current problems faced by farmers. The survey was conducted in South Korea during the period January, 2008 to August, 2012 in order to shed light on prevalence of different diseases on watermelon in different regions and to better understand farmers' knowledge and perceptions for following watemelon's growing procedures and practices. A total of 590 cases were reported on 573 watermelon growers with highest 87.1% in Jeonbuk, 4.7% in Jeonnam, and 8.2% in other regions such as Gyeongbuk, Gyeongnam, Chungnam, Gwangju, Gyeonggi, Daegu, Gangwon, Changwon and Seoul. The maximum percentages of cases recorded were related to diseases and insect pest (38.6%) followed by physiological disorder (29.7%), cultural practices (18.8%), soil and fertilizer (9.0%), herbicide (2.9%) and others (1.0%). It was observed that the manifestation aspects of the diseases were changed due to increasing in proportion of 'in-facility' cultivation to 12,995 ha compared to 'open-field' cultivation (2,722 ha). The present survey revealed the necessity to reduce the damages incurred at watermelon farms as soon as possible through the breeding program to develop resistant cultivar, use of pathogen-free propagating plants, and efficient prevention of pathogen by regular monitoring of watermelon plants at farms.

Diversity and Physiological Characteristics of Culturable Bacteria from Marine Sediments of Ross Sea, Antarctica (남극 로스해 퇴적물로부터 분리된 세균의 다양성 및 생리학적 특성)

  • Lee, Yung Mi;Jung, You-Jung;Hong, Soon Gyu;Kim, Ji Hee;Lee, Hong Kum
    • Korean Journal of Microbiology
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    • v.50 no.2
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    • pp.119-127
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    • 2014
  • The affiliations and physiological characteristics of culturable bacteria isolated from the sediments of Ross Sea, Antarctica were investigated. Sixty-three isolates obtained by cultivation were grouped into 21 phylotypes affiliated with the phyla Actinobacteria and Bacteroidetes and with the classes Alphaproteobacteria and Gammaproteobacteria by phylogenetic analysis of 16S rRNA gene sequences. Based on phylogenetic analysis (<98.65% sequence similarity), approximately 49% of total isolates represented potentially novel species or genus. Among them, extracellular protease, lipase, and exopolysaccharide activities at $10^{\circ}C$ or $20^{\circ}C$ were detected in approximately 46%, 25%, and 32% of the strains, respectively. Forty-three isolates produced at least one type of extracellular material and 21 of them produced at least two extracellular protease, lipase, and/or exopolysaccharides. Our findings indicate that culturable bacterial diversity present within the marine sediments of Ross Sea, Antarctica may contribute to the hydrolysis of the major organic constituents which is closely related with carbon and nitrogen cycling in this environment.

Testing a Simple Cultivation Method using Jew's Mallow(Corchorus olitorius L.) for Evaluating Biological Quality of Upland Soils (Jew's mallow (Corchorus olitorius L.)의 유식물(幼植物)을 이용(利用)한 밭 토양(土壤) 미생물성(微生物性) 간이(簡易) 평가(評價) 방법(方法))

  • Okano, Seigo;Kwon, Jang-Sik;Suh, Jang-Sun;Arao, Tomohito
    • Korean Journal of Soil Science and Fertilizer
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    • v.32 no.1
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    • pp.84-89
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    • 1999
  • A simple cultivation method using Jew's mallow (Corchorus olitorius L.) was examined to evaluate the biological quality of 72 upland soils collected from greenhouses in Korea. Each soil was mixed with 4-fold volume of sterilized basal soil having no indigenous chemical and physical problems to lessen the chemical and physical variations among original sample soils. This study assumes that the biological characteristics of each mixture 2 weeks after treatment reflect those of individual sample soil prior to treatment. Jew's mallow (Corchorus olitorius L.) was cultivated for 4 weeks in a pot filled with 250g of each experimental soil and the biological quality of each soil was estimated by the aboveground plant growth. The results indicated that microbial floral quality was explicitly deteriorated in 18 soils having EC above or equal to $5dS\;m^{-1}$. However, in soils having EC below $5dS\;m^{-1}$, some showed a sign of deterioration, while others did not. As this simple method is a kind of bioassay, it dose not require the measurements of microbial numbers and activities.

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