• 인간식물환경학회지
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• 제23권2호
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• pp.159-170
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• 2020

The purpose of this study was to understand the current status of education and programs and to identify the items that urban residents need most for garden management. First of all, there were 45 sites (40.5%) with gardens operated by city and county local governments nationwide, and 41(91.1%) of them had dedicated staff (1.8 persons on average). The average area of gardens was 18,623 m², garden area per person was 20.27m², the average number of participants was 683, and the average period of use was 8.69 months. In addition to gardening activities, 14 (31.1%) out of 45 sites were operating small group meetings, with an average of 2.29 meetings and 67 participants. In the satisfaction survey after gardening activities, 88.9% of 18 sites were satisfied. According to the questionnaire about education and programs related to garden users, an average of four sessions were conducted per education. In terms of education, the contents were in the following order: basic education on garden cultivation (33.9%) > prior education on garden operation (28.9%) > pest control (14.0%) > eco-friendly management (11.6%) > pesticides and Positive List System (9.9%) > others (1.7%). Over 95% of the respondents were generally satisfied. Regarding the perception survey on which items are needed to develop garden management guidelines, the most necessary items were in the order of crop management (38.7%) > public garden etiquettes among users (27.9%) > pest control (14.4%) > weed management (13.5%) > activities using harvest (5.4%). The contents that are to be included in the guidelines were in the order of garden planning and crop selection (17.2%) > cultivation techniques and schedule (16.5%) > pest and soil management (15.7%) > introduction of garden crops and gardening models (12.7%) > garden etiquettes (10.7%). In establishing urban garden management plans by region, the results will have high utility value as the basic data for continuous garden operation by setting a direction that meets the regional characteristics as well as the needs of urban residents.

• Mycobiology
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• 제50권2호
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• pp.104-109
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• 2022

Fungi of the genus Tuber are ectomycorrhizal fungi that form a symbiotic relationship mainly with oak and hazel trees. Tuber spp. exhibit a highly selective host plant preference; thus, for cultivation purposes it is important to select an appropriate host plant for successful mycorrhization. In addition, as mycorrhizal characteristics differ according to Tuber spp., it is necessary to understand the differences in mycorrhizae according to the fungal species. Tuber huidongense and Tuber himalayense were recently discovered in Korea; therefore, we used spore suspensions from these two species to inoculate two species of oak trees, Quercus acutissima and Quercus dentata, to compare colonization rates and morphologies of the mycorrhizae. The colonization rates demonstrated that the different Tuber spp. favored different host plant species. In addition, unique morphological and anatomical characteristics were observed for T. huidongense and T. himalayense depending on the host species. These findings can lead to new economically important agricultural activities related to truffle cultivation in Korea.

• 한국자원식물학회지
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• 제28권1호
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• pp.126-134
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• 2015

LED 광원을 달리하여 재배한 감초지상부의 항산화, 항돌연변이원성을 평가하였다. BL-0에서 DPPH 라디칼 소거활성에서 $EC_{50}$이 $3.02{\pm}0.13{\mu}g/ml$와 ABTS 라디칼 소거활성은 $EC_{50}$이 $2.18{\pm}0.18{\mu}g/ml$와 환원력이 가장 높게 나타났다. 폴리페놀함량은 $2.93{\pm}0.11g/100g$으로 가장 높게 나타났으며, RL-0에서는 항산화 활성이 가장 낮게 나타났다. 항돌연변이원성 평가에서 직접변이원인 1-NP에 대해 모든 실험군에서 돌연변이 억제효과를 나타냈으며, 간접변이원인인 Trp-P-1, Trp-P-2, $AFB_1$에 대해서는 BL-0에서 가장 높은 돌연변이 억제효과를 나타냈고, 2-AA에서는 RL-1에서 돌연변이 억제가 높게 나타났다. 청색광 LED (650 nm)와 적색광 LED (450 nm)를 이용하여 재배한 감초지상부의 성분변화를 관찰하기 위해 GC/MS를 이용해 분석한 결과 25분에서 RL-1에서만 cytidine이 검출되었고, 37분에서 BL-0에서만 2-bromotrimethylene glycol이 특정적으로 검출되었다. 이는 LED광원 조건에 따라 감초 경엽의 성분이 차이가 있음을 보여준다. 이 성분들이 생리활성에 어떤 영향을 주는지는 더 검토해야할 부분이다.

• 한국작물학회지
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• 제62권3호
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• pp.203-213
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• 2017

본 연구는 팥을 벼 대체작물로 논 토양에 재배하기 하였을 경우 팥 품종별 생육, 수량, 습해정도, 종자의 무기성분, 항산화성분 및 항산화활성 성분을 평가한 결과는 다음과 같다. 1. 토양 수분은 팥 재배기간 동안 2015년에는 과습했던 일수가 16일었으나 2016년에는 49일이었고 평균 토양 수분도 2016년이 두 배정도 더 높았다. 2. 2015년과 2016년 팥의 수량을 비교한 결과 평균적으로 충주팥과 검구슬, 칠보팥이 높은 수량을 보였다. 즉 과습한 논 토양 환경에서도 수량 감소가 다른 품종들보다 적었다. 3. 품종간 종자의 일반성분 및 무기성분 함량은 크게 나타나지 않았다. 반면 항산화성분 중 총 폴리페놀과 플라보노이드 함량은 새길이 다른 품종들보다 확연히 높았고 검구슬이 다음으로 높았다. 낮은 함량을 보인 품종은 중부팥이 가장 낮았고 경원팥, 칠보팥이 다음으로 낮았다. 활성화 성분 함량은 역시 새길이 가장 높았고 검구슬이 다음으로 높았다. 4. 항산화성분과 활성화 성분 함량은 더 과습한 토양에서 재배된 팥들이 모든 품종에 걸쳐 증가하는 경향을 보였고 이 중 새길이 가장 큰 항산화성분 증가률을 보였다. 5. 여러 가지 측정된 변수들을 바탕으로 PCA 및 군집분석을 한결과 항산화성분과 활성화 성분이 시험품종들을 그룹으로 나누는데 영향이 가장 큰 변수로 나타났고 이에 따라 새길만이 확연한 차별화를 보였다. 이상의 결과를 종합해 보면 논 토양에서 또는 과습한 토양 상태에서 수량 관점으로는 충주팥, 칠보팥, 검구슬 같은 팥 품종이 수량 감소가 적었고 종실성분 향상을 고려할 경우 새길이 더 우수하다는 결과가 나타났다. 추후 팥이 습해에 대한 반응을 뿌리 또는 생리적 반응까지 고려하여 분석하고 이를 변수로 추가하여 토양 수분과 팥의 상관관계가 명확하게 나타날 것으로 본다.

• 농촌계획
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• 제25권4호
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• pp.57-64
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• 2019

The purpose of this study was to investigate the general characteristics of the elderly who participate in the planting activities of weekend farms and the effect of the planting activity levels on the subjective health, depression, and human relationships of the elderly. Data were collected and analyzed using subjective health, depression, and human relationship measures that can represent physical, mental, and social health. The main results were as follows. The educational level, the status of spouses, and the number of diseases of the general characteristics of the elderly who participated in the weekend farm showed significant differences in all subjective health, depression, and human relationships. Plant cultivation activity level was positively correlated with the subjective health of the elderly and had a significant effect. The higher the level of plant cultivation activity, the more positively it affected the human relationship. Plant cultivation activity was negatively correlated with the depression of the elderly.

• 농약과학회지
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• 제3권1호
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• pp.66-77
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• 1999

Bipyridylium계 제초제 paraquat 잔류물의 토양중 행적을 구명하기 위하여 [$^{14}C$]paraquat의 신생 및 6주간의 숙성잔류물을 함유한 2종의 토양을 가지고 특수하게 제작한 pot에서 옥수수를 4주간 재배하였다. 숙성기간과 옥수수 재배기간 동안 $^{14}CO_{2}$로 무기화된 양은 총처리 방사능의 각각 $0.13{\sim}0.18%$와 $0.02{\sim}0.17%$이었다. 옥수수 재배기간중 뿌리와 줄기에 흡수 이행된 $^{14}C$은 두 토양 모두에서 각각 0.1%와 0.01% 미만이었으나 토양중에 남아 있는 $^{14}C$은 두 토양 모두에서 총처리 방사능의 97% 이상이었다. 4주간 옥수수를 재배한 후 토양중 paraquat의 물에 의한 추출율은 1.2% 이하로 매우 낮았고 [$^{14}C$]paraquat 추출불가 토양잔류물의 대부분은 humin에 혼입되어 있었다. 토양을 숙성하는 동안 토양 B의 pH가 증가하였고 옥수수 재배후에는 모든 처리구에서 토양의 pH가 증가하였다. 옥수수뿌리의 각 subcellular particle중에서 residue 분획(incompletely homogenized tissue)에 가장 많은 $^{14}C$이 분포해 있었다. 토양의 dehydrogenase 활성은 숙성에 관계없이 옥수수 재배후에 증가하였다.

• Journal of Applied Biological Chemistry
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• 제60권2호
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• pp.149-153
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• 2017

원목과 톱밥배지에서 푸른곰팡이의 오염과 성장은 표고버섯의 생장을 심하게 저해할 수 있다. 표고버섯 재배에서 푸른곰팡이병의 방제를 위하여 농약과 항생제와 같은 화학 약품의 사용은 일반적으로 허용되지 않는다. 본 연구에서는 푸른곰팡이병의 방제를 위하여 느타리버섯에서 갈반병을 일으키는 세균성 병원균을 분리하고 이들의 펩티드 독소를 분리하였다. Pseudomonas tolaasii 균주들은 항진균 활성을 갖는 톨라신 및 이와 구조적 유사체인 다양한 펩티드 독소를 분비한다. 이러한 펩티드들은 표고버섯의 생장에는 영향을 주지 않는다는 것이 알려져 있다. 펩티드 독소들을 Trichoderma harzianum H1 푸른곰팡이에 처리하였을 때, 푸른곰팡이의 성장을 저해하였다. 펩티드를 분비하는 20 종의 P. tolaasii 균주 중에서 강, 중, 약의 항진균 활성을 가진 균주의 수는 각각 8, 5, 7 종으로 나타났다. 표고버섯을 재배하는 동안에, 펩티드 독소를 함유한 세균 배양액에서 세균을 제거한 배양원액을 톱밥배지 표면에 자란 푸른곰팡이 균사에 분무하였다. 배양원액은 푸른곰팡이의 성장은 억제할 수 있는 반면, 표고버섯 생장과 생산에는 영향을 주지 않았다. 펩티드 독소를 함유하는 배양원액은 곰팡이의 흰색 균사체를 노란색의 마른 딱지로 변화시켰고, 이것은 펩티드 독소가 강력한 항진균 활성과 살균 활성을 갖고 있음을 보여준다.

• 대순사상논총
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• 제26집
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• pp.183-214
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• 2016

The purpose of this study is to promote the understanding of the objective of Daesoon Thought through 'examining the Objectives of Daesoonjinrihoe.' The objective of Daesoon thought was set when Kang Jeungsan Sangje descended to human world, upon the plea of divine sages, buddhas, and bodhisattvas, and determined to save the world and relieve people far and wide. For building an Earthly Paradise with Daesoon thought, Sangje carried out the great work of Reordering of the Universe for nine years and passed into heaven. Cho Jeongsan Doju, who received the heavenly religious orthodox through divine revelation from Sangje, served the late will of Sangje and established Main Tenets, Creeds, and objective of Daesoon thought. The objective set by Doju was continued to Park Wudang Dojeon and formed the Objectives of Daesoonjinrihoe. The Objectives of Daesoonjinrihoe consist of three sets of objectives in sequential order, which are related in organic, mutual beneficient, and integrative with one another. The Objectives starts from individual cultivation and expand into worldly reformation: 1)one's Spiritual transformation through Guarding against self-deception 2)Renewal of human beings through Realizing Earthly Immortality 3)Opening of new world through Building of Earthly Paradise. Also, in these Objectives of Daesoonjinrihoe, Three Realms of Heaven, Earth, and Humanity keep close relations to enter into new world. These Objectives are based on the 'thought of mutual beneficence' and are realized with Daesoon thought, which is the Dao of 'Crowned King' that goes beyond the previous thoughts of Confucianism, Buddhism and Taoism. Objectives of Daesoonjinrihoe have following details in each objective as following; (1) 'Guarding against self-deception-Spiritual Transformation' is for human nature. In Daesoon thought, human has the potential to restore conscience, which is true nature, and return to undefiled essence by 'guarding against self-deception'. In other words, it views that one can reach into the stage of spiritual alignment with the Dao once he recovers conscience through cultivation of Daesoonjinrihoe based on the idea of 'Guarding against self-deception'. (2) 'Realizing Earthly Immortality-Renewal of human beings' is for ideal human character. 'Realizing Earthly Immortality' in Daesoon Thought indicates the advent of ideal human beings called 'Dotong gunja'(virtuous beings who are aligned with the Dao). They are leaders who integrate politics and religion in helping people to renew themselves and make the world into Earthly Paradise. (3) 'Building an Earthly Paradise-Opening of new world' is for ideal society. Daesoon thought aims to making a peaceful world by creating an ideal society of heaven on this earth. Park Wudang Dojeon, who led Daesoonjinrihoe, emphasized the importance of Three Basic Activities: Spreading of Sangje's virtue, Edification, and Cultivation: and Three Major Activities: Aid and charity, Social welfare, and Education. Dojeon said "One should make effort to reach the utmost goodness through enlightening their bright virtue and cultivating their talent virtue." In this words, he made clear that the Objectives of Daesoonjinrihoe can be realized when people enlighten their bright virtue and cultivate themselves with their talent virtue. In conclusion, the Objectives of Daesoon thought were clarified of their meanings by Sangje, established into laws by Doju, and actualized by Dojoen in various activities of Daesoonjinrihoe.

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.156-163
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• 2017

A greenhouse experiment was conducted for evaluation of ecological effects of transgenic melon plants in the rhizospheric soil in terms of soil properties, enzyme activities and microbial communities. Organic matter content of soil under transgenic melon plants was significantly higher than that of soil with non-transgenic melon plants. Significant variations were observed in organic matter, total P and K in soil cultivation with transgenic melon plants. There were also significant variations in the total numbers of colony forming units of fungi, actinomycetes and bacteria between soils treated with transgenic and non-transgenic melon plants. Transgenic and non-transgenic melon significantly enhanced several enzymes activities including urease, acid phosphatase, alkalin phosphatase, arysulphtase, ${\beta}$ glucosidase, dehydrogenase, protease and catalase. Soil polyphenoloxidase activity of $T_1$ transgenic melon was lower than that of $T_0$ transgenic melon and a non-melon plant during the same period. The first generation transgenic melon plants ($T_0$) showed significantly greater (p<0.05) effect on the activitiy of arylsulfatase, which increased from $2.540{\times}10^6CFU\;g^{-1}$ (control) to $19.860{\times}10^6CFU\;g^{-1}$ ($T_0$). These results clearly indicated that transgenic melon might change microbial communities, enzyme activities and soil chemical properties.

• Journal of Applied Biological Chemistry
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• 제60권1호
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• pp.41-47
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• 2017

Genetic differentiation and antioxidant activities in ethanolic extracts from leaves of Bouea macrophylla Griffith were determined. The result revealed genetic differentiation among sour ma-praang, ma-yong and sweet ma-praang of B. macrophylla Griffith (${\phi}_{PT}=0.772$, p-value=0.000). In addition, high genetic diversities were found in sour ma-praang and sweet ma-praang populations (P: 51.4 and 57.1 %; He: 0.1900.035 and 0.2240.036, respectively), but low genetic diversity was found in ma-yong population (P: 8.6 %; He: 0.0350.021). Total phenolic contents of sour ma-praang, ma-yong and sweet ma-praang were estimated as $680.51{\pm}89.81$, $701.03{\pm}59.89$ and $530.85{\pm}41.23mg$ gallic acid/g extract, respectively. Free radical scavenging activities of sour mapraang, ma-yong and sweet ma-praang were found (1/EC50=4.17, 1.43 and 1.37, respectively) corresponding with metalchelating activities (1/EC50=0.83, 0.65 and 0.17, respectively). Therefore, the obtained data may be applied to cultivation and utilization of their leaves as source of natural phenolics and antioxidants.