• 한국약용작물학회지
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• 제14권4호
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• pp.202-205
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• 2006

인삼의 안정적인 생산 및 합리적인 병해방제에 기여하기 위하여 연구를 실시한 결과 갈잎 퇴비에서 분리된 Streptomyces lauretii B8180과 Bacillus subtilis B8856, Burkholderia cepacia B7944 세 균주의 인삼병원균 5종에 대한 항균력이 인정되었으며, 특히 실내실험을 통하여 높은 역병 방제효과가 인정되었다. 그러나 이 실험에서와 같이 식물의 지상부에 길항균을 살포하는 방법을 통하여 역병 방제효과를 높일 수 있을 것으로 생각된다. 이 길항균들은 갈잎 퇴비에서 분리되었기 때문에, 갈잎 퇴비에서 증식시켜 인삼재배 예정지에 투입하면, 억제형 토양 (suppressive soil) 조성을 통한 주요병해 방제가능성도 클 것으로 생각된다.

• 한국식물병리학회지
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• 제14권5호
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• pp.491-495
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• 1998

A fungal disease of the cultivated mushroom, Ganoderma lucidum, caused by Xylogone sphaerospora was epidemic throughout all cultivation areas in Korea which caused a lot of yield losses in the mushroom production. For controlling the disease, the screening of effective fungicides against the pathogenic fungus were conducted. Thirty seven commercially available fungicides were tested for their inhibitory activities on potato dextrose agar media supplemented with these fungicides at various concentrations. Twenty one fungicides significantly inhibited mycelial growth of the pathogen, Xylogone sphaerospora, but 16 fungicides had no inhibitory effect. Among these 21 fungicides, 17 fungicides also inhibited mycelial growth of Ganoderma lucidum as well, but imazalil, procymidone, triforine, and vinclozolin had no inhibitory effects. However, vinclozolin showed no inhibitory effect on mycelial growth of the mushroom even at the concentration of 50 $\mu\textrm{g}$/ml vinclozolin solution for 2 hours, and then the pathogen was inoculated. After two month-cultivation of the mushroom, over 90% of logs treated with vinclozolin without pathogen inoculation produced fruiting bodies. However, fruiting bodies were not produced form the logs inoculated with the pathogen, but not treated with vinclozolin. Fifty seven percent of logs. which were pre-treated with vinclozolin and then inoculated with the pathogen produced fruiting bodies. Based on the results, vinclozolin is effective for the control of yellow disease of the Ganoderma lucidum caused by Xylogone sphaerospora.

• 한국미생물·생명공학회지
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• 제24권4호
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• pp.459-464
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• 1996

Exo-polysaccharide (BWS) obtained from submerged cultivation of Ganoderma lucidum mycelium was fractionated. Antitumor activity of their fractions was investigated in comparison with the mycelial polysaccharide fractions. Eight kinds (BWS-DN, BWS-DA, BWS-DN-GI, BWS- DA-GI, MWS-DN, MWS-DA, MWS-DN-GI and MWS-DA-GI) of polysaccharide fractions were obtained by DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel chromatography from BWS and MWS, which were isolated from culture fiuid and mycelial cell, respectively. The anticomplementary activities (ITCH$_{50}$%) of the exo-polysaccharide fractions showing 15% to 30% were lower than those of mycelial polysaccharide fractions showing 15% to 70%. The acidic fractions of BWS-DA and BWS-DA-GI fractionated from BWS, showed the highest activity of 30%. In the MTT assay, BWS-DN and MWS against mouse leukemia L1210 exhibited high inhibition ratio of 86 and 89%, respectively at the concentration of 600 $\mu$g/ml. High inhibition ratio of 50% (IC$_{50}$) was achieved for BWS, BWS-DA and MWS-DA fractions against human colon adenocarcinoma COLO-205 and for BWS-DA, BWS-DN and MWS-DN fractions against human leukemia HL-60 at the concentra- tion of 300 $\mu$g/ml among the six polysaccharide fractions, respectively.

• 디지털융복합연구
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• 제17권3호
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• pp.55-61
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• 2019

본 연구는 올바른 창의 인성 함양을 위한 동요지도를 멀티미디어 자료로 개발하여 효과를 살펴보고자 했다. 이를 위해, 초등학생을 대상으로 인성 함양에 적합한 동요를 선별하고, 애니메이션이나 다큐멘터리, 합창이나 무용 공연, 인터뷰 등 다양한 영상 자료를 포함한 멀티미디어를 제작하고, 제작된 자료를 초등학교에서 동요방송으로 운영하였다. 무엇보다, 교사들이 학생들에게 동요를 쉽게 지도할 수 있도록 교수 학습안과 활동지를 프로그램으로 고안하여 재량활동 시간을 통해 '창의적인 교수 학습 활동안'으로 프로그램이 진행되었고, 학급 홈페이지를 통해 가정에서도 연계할 수 있도록 제시하였다. 그 결과, 동요방송을 통해 초등학생의 인성변화가 긍정적으로 나타났고, 초등학교 시기에 가장 강조되어야 할 창의 인성 함양을 위한 실제적인 활용방안을 통합적인 접근방법에서 논의하고자 했다.

• 한국자원식물학회지
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• 제25권6호
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• pp.657-663
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• 2012

Muscle strength and endurance activities of Korean ginseng (Panax ginseng C. A. Meyer; KG) were compared with those of wild simulated cultivation ginseng (WCG) in mice. Fifty male ICR mice were divided into five groups: A (vehicle); B (WCG 100 mg/kg); C (WCG 500 mg/kg); D (KG 100 mg/kg); E (KG 500 mg/kg). Subsequently, the mice were subjected to the forced swimming test (FST) and treadmill test at the $4^{th}$ and $7^{th}$ weeks. The glycogen content in the muscle and blood analysis (levels of glucose, triglyceride (TG), IGF-1) were also performed immediately after the last FST and treadmill test at the $7^{th}$ week. Immobility times in FST were shorter in WCG- than KG-treated groups, and the results of the treadmill tests were also significant except for KG-treated at 100 mg/kg. The glycogen content was increased in both groups with a peak at 500 mg/kg of WCG groups. Serum concentrations of TG and glucose were decreased by administration of KG and WCG and all treated groups showed increase in the level of IGF-1 in serum. These results suggest that KG and WCG supplementations are effective in escalating the muscle strength and endurance.

• 원예과학기술지
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• 제34권4호
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• pp.634-643
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• 2016

본 연구에서는 제주와 오키나와에서 생산되는 망고 'Irwin' 과실에 대해 품질 및 기능성을 비교 평가 하기 위해 가용성 고형분, 총 페놀 및 플라보노이드 화합물, mangiferin 등 원료특성과 에탄올 추출물의 항산화 활성을 비교해 보았다. 망고 'Irwin' 을 저수고 유인재배를 하는 오키나와 농가하우스와 온난화 대응농업연구소(IRCA)가 제주농가 하우스 보다 가용성 고형분 함량과 항산화 활성 및 mangiferin 함량이 높았으며, 오키나와 농가하우스가 IRCA 보다 이들 함량이 높았다. 이들 결과는 망고 'Irwin' 에 있어 저수고 유인재배가 과실의 품질향상에 중요하다는 것이 시사가 되었다.

• 한국토양비료학회지
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• 제49권2호
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• pp.144-156
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• 2016

Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

• 대한한의학회지
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• 제27권1호
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• pp.23-35
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• 2006

Objectives : This study was conducted to investigate the inhibitory effects of Gaejibokryunghwan on cell proliferation in HeLa cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Gaejibokryunghwan extract solution. All three were cultured for 24 hours, 48 hours and 72 hours each, to examine the inhibitory effects of Gaejibokryunghwan. Afterwards, we drew out the effect of Gaejibokryunghwan extract solution by making 5 analysis. First analysis was to measure the proliferation rate of cells. Second was FACS analysis. Third was to estimate the activity or caspase-3. Fourth, we used XTT assay to analyze the activation or cells. Ana lastly, a molecular biological method was used to determine activation of MAP kinase in the HeLa cells. Results : After 24, 48 and 72 hours cultivation, the proliferation of HeLa cells showed the dose-dependent decrease in all Gaejibokryunghwan extract solution groups compared to the control group. In the FACS analysis, Gaejibokryunghwan extract solution groups showed increased caspase expression compared to the control group, except for the group for 48 and 72 hours in 1 % concentrate. Caspase-3 activities were increased in all, except tile group cultured for 24 hours in 5% concentrate and the groups cultured for 48 hours in 1% and 5% concentrate. In the XTT study, 1% Gaejibokryunghwan extract solution groups showed increase compared to the control group, but other Gaejibokryunghwan extract solution containing groups showed significant decrease compared to the control after 24, 48 and 72 hours of cultivation. The expressions of MAP kinase were decreased in all Gaejibokryunghwan extract solution containing groups compared to the control group after 24, 48 and 72 hours of cultivation. Conclusions : From this study, we could suggest that Gaejibokryunghwan be available to the inhibition of proliferation of human cervical carcinoma cell line, HeLa cells in vitro.

• 생약학회지
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• 제43권3호
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• pp.231-238
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• 2012

Menthae herba (MH) extracts exhibit anti-inflammatory effects. The purpose of this study was to determine whether the anti-inflammatory effects of MH extracts vary according to the cultivation regions. We performed a comparative analysis of MH extracts by evaluating the production of inflammatory mediators in RAW 264.7 murine macrophage cells and HaCaT human keratinocyte cells. MH extracts obtained from different cultivation regions in Korea and China significantly reduced the production of nitric oxide (NO), prostaglandin $E_2$ ($PGE_2$), and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). No differences in these inhibitory activities were observed between MH extracts. In HaCaT cells stimulated with TNF-${\alpha}$ and interferon-${\gamma}$ (IFN-${\gamma}$), MH extracts did not inhibit the production of macrophage-derived chemokine (MDC/CCL22), but most extracts reduced the production of the regulated on activation normal T-cell expression and secreted (RANTES/CCL5). We used clustering tree analysis of the MH extracts according to the chromatographic pattern and anti-inflammatory potency of MH extracts. We observed differences in the chromatographic pattern of MH extracts but no difference in anti-inflammatory potency. Our findings suggest that MH extracts from different regions do not show any differences in their pharmacological potency in that MH extracts are used as therapeutic agents to treat inflammatory disorders.

• 한국환경농학회지
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• 제36권4호
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• pp.223-229
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• 2017

BACKGROUND: Tillage systems and fertilization play an important role in crop growth and soil improvement. This study was conducted to determine the effects of tillage and fertilization on the microbial biomass C and dehydrogenase activity of soils in a field under cultivation of soybean. METHODS AND RESULTS: An experimental plot, located in the temperate climate zone, was composed of two main sectors that were no-tillage (NT) and conventional tillage (CT), and they were subdivided into four plots, respectively, in accordance with types of fertilizers (non fertilizer, chemical fertilizer, hairy vetch, and liquid pig manure). Microbial biomass C and dehydrogenase activity were evaluated from May to July in 2016. The microbial biomass C and dehydrogenase activity of NT soils were significantly higher than those of CT in all fertilizer treatments, and they were further increased in hairy vetch treatment than the other fertilizer treatments in both NT and CT. The dehydrogenase activity was closely related to microbial biomass C. CONCLUSION: It is concluded that application of green manure combined with no-tillage can provide viable management practices for enhancing microbial properties of soil.