Kim, Yangseon;Kang, In Jeong;Shin, Dong Bum;Roh, Jae Hwan;Heu, Sunggi;Shim, Hyeong Kwon
Research in Plant Disease
/
v.23
no.3
/
pp.278-282
/
2017
Maize is one of the most cultivated cereals as a staple food in the world. The harvested maize is mainly stored after drying, but its quality and nutrition could be debased by fungal spoilage and mycotoxin contamination. In this study, we surveyed mycotoxin contamination fungal infection of maize kernels that were stored for almost one year after harvest in 2015. The amount of deoxynivalenol and zearalenone detected were higher than the other mycotoxin, such as aflatoxin, ochratoxin, fumonisin and T-2 toxin. In particular, level of deoxynivalenol was detected as $1200{\pm}610{\mu}g/kg$ in small size kernels, which was four to six times higher than the large and the medium size kernels. Moreover, the amount of deoxynivalenol, zearalenone, and fumonisin were increased with discolored kernels. 10 species including Fusarium spp., Aspergillus spp. and Penicillium spp. were isolated from the maize kernels. F. graminearum was predominant in the discolored kernels with detection rates of 60% (red) and 40% (brown). Our study shows that the mycotoxin contents of stored maize can be increased by discolored maize kernels mixed. Therefore elimination of the contaminated maize kernels will help prevent fungal infection and mycotoxin contamination in stored maize.
The pathogenicity of free-living amoeba, Waegleria fcwleri, is influenced according to the strain, cultural condition and host (Culbertson et at., 1968; Carter, 1970; Wong et at., 1975), Phillips (1973) demonstrated that Entamoeba histolytica became avirulent after more than 2 year maintenance in axonic culture in vitro. This study was carried out to compare the difference in pathogenicity between two strains of N. fowleri, one of a prolonged maintenance in arsenic medium and the other one obtained by serial brain passage in mice. The 0 strain was that N. fowleri had cultivated axenically more than 7 years in CGVS medium. The 2-1 strain was obtained from the brain of mouse inoculated intranasally with a strain, which was from the mouse brain infected with 0 strain, and cultured for 15 weeks until the beginning of this experiment. White male mice weighing 18-22 g were used. Mice were anesthetized by an intraperitoneal injection of about 1 mg secobarbital, and inoculated intranasally with $10{\times}$10^4 live N. fowleri trophoBoites in a $5{\;}{\mu}l$ cell suspension. Sluggish behaviour, nervousness, rotation and leg paralysis were developed earlier and more frequently in the 2-1 experimental group than the control 0 group. Pathological changes such as inflammatory and necrotic lesion were observed in the olfactory and anterior portion of brain, and these changes were more extensive in the 2-1 group. The edematous and inflammatory changes in lung were demonstrated in mice died after 13th day post-inoculation. The experimental mice of 2-1 group began to die suddenly from 7th day post-inoculation, and the survival time in 2-1 group mice was shorter than 0 group mice. The typical primary amoebic meningoencephalitis was developed in the mice inoculated intranasally with N. fowleri. The prolonged maintenance of N. fowleri amoebae in axonic CGVS medium was observed to have lost their original pathogenicity for mice, but their pathogenicity was restored by serial brain passage in mice.
Recently, agricultural lands have decreased sharply, which was caused by huge housing site, urbanization, land consolidation, and road construction etc. In particular, Yangju city near Seoul city has the most severe land use change in Korea. Therefore, we analyzed changes of land use, soil properties, and soil information in order to provide the basic soil information and soil management practices in this city. The area of crop cultivated land in Korea (2015) reduced by 12,090 ha compared to ones from the previous year (2014). The paddy field decreased by 25,421 ha but, upland field increased by 13,331 ha. One of the reasons for the reduction of the paddy field was converting paddy field to upland (20,916 ha) > others (3,056) > building (2,571) > public facilities (847) > idle land (217). But, reasons for increase of upland field were switching paddy to upland (20,916 ha) > land developed (634). The main reason of converting paddy field to upland was changing from rice to more profitable speciality crops or pulses. The cropland area (paddy fields, upland, orchard) of Yangju city reduced by 1,412 ha (2015/2014). The ratio of cropland area in each city reduced by 22.9% dramatically compared 2015 to 1999. The paddy fields located in alluvial plains in Yangju city were changed into upland or green house. The drainage classes of soil have been deteriorated because the flows of water were intercepted by road construction and other disturbance to water flows. In particular, paddy fields have been changed to not only upland, orchard, greenhouse cultivation but also to fallow and soil dressing on paddy in Yangju city. To analyze result of soil survey of Yangju city, 858 soil codes (soil phases) were used and the area was 105.17ha. The number of soil series increased from 60 to 65, and that of soil phase increased from 105 to 124. The largest increased area was Noegog soil series. 125.7ha of Neogog soil series was incorporated from the existing Sachon, Yecheon and Eungog soil series. The soil suitability class of paddy field in Ogjung huge housing site of Yangju city was the 4th grade for 32.6% of the area. The soil suitability classes of upland were 2nd and 3rd grade for 72.4% of the area. Farm land with high quality should be conserved by related law.
Choi, Man Kwon;Yun, Sung Wook;Yu, Chan;Lee, Si Young;Yoon, Yong Cheol
Journal of Bio-Environment Control
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v.22
no.3
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pp.234-240
/
2013
The purpose of this study was to provide basic data used in greenhouse design, and for this purpose, the crop loads of tomato and paprika which have high crop load among crops cultivated in greenhouse were measured. The maximum differences in lowest and mean temperatures between cultivation year and normal years for winter season were -5.6 and $-4.9^{\circ}C$, respectively and those for summer season were 3.0 and $1.5^{\circ}C$, respectively. The calibration of push & pull gauge indicated approximate 0.99 of coefficient of determination, representing very high association. The planting densities of paprika and tomato were 3.43, 3.56 and $2.13plants{\cdot}m^{-2}$, respectively. The yields were 4.1, 4.3 and $16.2kg{\cdot}plant^{-1}$, corresponding to 14.1, 15.2 and $34.5kg{\cdot}m^{-2}$ of yield per unit area. The maximum loads were 1.3, 1.5 and $3.3kg{\cdot}plant^{-1}$, respectively. When the paprika were hanged on cross beam, the total crop load applied to it was estimated to be $15.3{\sim}15.9kg{\cdot}m^{-2}$, and when the weights of gutters supporting the cultivation beds, the total weights were $26.0{\sim}26.9kg{\cdot}m^{-2}$.
Seo, Young-Ho;Kim, Se-Won;Choi, Seung-Chul;Kim, In-Jong;Kim, Kyung-Hi;Kim, Gun-Yeob
Korean Journal of Soil Science and Fertilizer
/
v.45
no.4
/
pp.540-543
/
2012
Atmospheric nitrous oxide ($N_2O$) level has been increasing at a rate of 0.2~0.3% per year. The rise in $N_2O$ concentration in atmosphere was mainly due to an increased application of nitrogen fertilizers. The objective of the study was to assess the effect of green manure crop and biochar on $N_2O$ emissions from upland crop field. The green manure crop used in the study was hairy vetch and the cultivated crop was red pepper (Capsicum annuum L.). Nitrogen was applied at a rate of $190kg\;ha^{-1}$, standard N fertilization rate for red pepper. Emissions of $N_2O$ from the field were reduced from the plots applied with hairy vetch and biochar by 46.5% and 24.6%, respectively, compared with nitrogen fertilizer treated plots with $N_2O$ emission of $1.14kg\;N_2O-N\;ha^{-1}$. The results from the study imply that green manure crop and biochar can be utilized to reduce greenhouse gas emission from the upland crop field.
Gadberry, Bradley A.;Colt, John;Maynard, Desmond;Boratyn, Diane C.;Webb, Ken;Johnson, Ronald B.;Saunders, Gary W.;Boyer, Richard H.
ALGAE
/
v.33
no.1
/
pp.109-125
/
2018
Turkish towel (Chondracanthus exasperatus), Pacific dulse (Palmaria mollis, also known as Red ribbon seaweed), and sea lettuce (Ulva spp.) were cultivated in a land-based intensive culture system at the Manchester Research Station, USA from August 2013 to September 2014. Macroalgae were grown in tumble-aerated tanks, harvested bimonthly for seasonal growth calculations, and analyzed for protein, lipid, ash, and amino acid content. Growth rate of all three species exhibited a similar pattern, with the highest specific growth rates occurring during the summer months (Turkish towel: 7.8%, Pacific dulse: 8.2%, and sea lettuce: 6.2%). Growth of all three species was lowest around winter solstice; with negative growth only observed in sea lettuce. On a dry weight basis significant differences in protein content existed between the three species with highest values for sea lettuce ($29.5{\pm}1.4%$). Lipid content varied between species (0.95-2.78%) with significantly higher lipid observed in sea lettuce (0.58-4.82%). No significant differences were detected on a seasonal basis among each species. Essential amino acids accounted for $43{\pm}0.9$ to $47{\pm}1.2%$ of total amino acids with Turkish towel having the highest value. Turkish towel had a significantly higher taurine level ($0.82{\pm}0.27$) than the other macroalgae. The levels of persistent organic pollutants and heavy metals were low. The estimated annual product of the three species ranged from 50- to $70-mt\;dry\;weight\;ha^{-1}\;y^{-1}$, significantly higher than conventional crops. Land-based culture of these species can produce year-round harvest, consistent product quality, and low contaminant levels.
Park, Yunmi;Kim, Mahn-Jo;Park, Sang-Byeong;Oh, Sung-Il
Korean Journal of Agricultural and Forest Meteorology
/
v.17
no.4
/
pp.340-347
/
2015
This study was conducted to analyze the relationship between climate factors and fruit characteristics of 'Sangjudungsi' persimmon (Diospyros kaki THUNB) has been mainly cultivated in Sangju of Korea. We has been surveyed the yield and fruit characteristics such as fruit weight and soluble solids of 'Sangjudungsi' persimmon from 2010 to 2015 for five years. Also, as major meteorological factor, mean air temperature, cumulative temperature and days, the data of sunshine duration, diurnal range, and rainfall were collected from Sangju Regional Meteorological Administration. As result of that, the annual variation of fruit weight was affected by sunshine duration for April and October related starting point of fruit weight increase. The content of soluble solids was affected by sunshine duration for April and October similarly with fruit weight and was negatively correlated with the number of days with precipitation in the year. These results indicate the characteristics related to fruit quality like the above were affected by the sunshine duration and precipitation which is critical factor for the intensity of radiation during rapid growth period (April and October). Fruit number and yield per tree was commonly affected by mean air temperature of April and cumulative temperature days of October. These results also indicate that temperature for period of rapid growth stage of persimmon may have an important role in the fruit number and yield per tree.
Moon, Byung Woo;Jung, Hae Woong;Lee, Hee Jae;Yu, Duk Jun
Korean Journal of Environmental Agriculture
/
v.32
no.2
/
pp.102-107
/
2013
BACKGROUND: Pithy pear fruit are not distinguished externally from sound fruit and thus often cause unexpected economic losses. To find out the cause of pithiness, the pithiness incidence and characteristics of Japanese pear (Pyrus pyrifolia cv. Niitaka) fruit picked from a spot frequently produced pithy fruit in an orchard were compared with those of fruit picked from another spot produced sound fruit every year. And the soil chemical properties of the two spots and mineral contents in fruit, shoots, and leaves of Japanese pear trees cultivated in the two spots were also examined. METHODS AND RESULTS: The pithiness incidence was 0, 8.8, and 11.3% at 7 days before and 0 and 7 days after optimal harvest date, respectively, in the spot frequently produced pithy fruit. Flesh firmness was significantly lower in pithy fruit than in sound fruit, while soluble solids content was slightly higher in pithy fruit than in sound fruit. Unlike other mineral contents, Ca content was significantly lower in pithy fruit than in sound fruit. These results indicate that Ca deficiency in fruit is closely associated with decrease in flesh firmness and thus pithiness development. Ca content in soil of the spot frequently produced pithy fruit was also significantly lower than that in soil of the spot produced sound fruit. However, shoots or leaves did not exhibit significant difference in Ca and/or other mineral contents between the two spots, indicating that Ca deficiency in fruit is dependent on the translocation of Ca within a plant rather than soil Ca status. Although total-N, available $P_2O_5$, K, and Ca contents were significantly lower in soil of the spot frequently produced pithy fruit than in soil of the spot produced sound fruit, Mg and Na contents and pH were not different between the soil conditions. CONCLUSION(S): Fruit maturity and Ca level in fruit are closely related to the incidence of pithiness in 'Niitaka' Japanese pear.
Two red pepper(Capsicum annuum L.) cultivars, 'Geumtap' and 'Bugang', were cultivated at main producing areas in Korea and harvested four times in 1998 and 1999. Length, weight, dry matter ratio and color of red peppers were measured at each harvest. The relations between external characteristics with regional climatic elements such as total accumulated air temperature, total amount of precipitation and duration of sunshine during the fruit growing periods were compared. Length and fruit weight decreased by delayed harvest. These characteristics were shown significant difference according to cultivars, but not to growing year. Dry matter ratio had a tendency to increase at the 3rd and 4th harvest. The red color as measured a${\times}$L values of pepper powder, increased with being later in harvesting, but the trend was uncertain. In fruit growth, fruit length showed a positive correlation with total accumulated air temperature in later growth period of fruits(from 1st to 20th day before harvesting), and fruit weight had relationships with accumulated air temperature and total amount of precipitation in later growth period of fruit. There was no relation between dry matter ratio and climate elements. The red color was shown high correlations with total amount of precipitation and duration of sunshine. There was highest coefficient of determination($r^2$) in all external characteristics.
Park, Jong-Dae;Uhm, Ki-Baik;Yoo, Jae-Gi;Kim, Sang-Chul
The Korean Journal of Pesticide Science
/
v.4
no.2
/
pp.50-55
/
2000
Occurrence, Injury aspects and control effect of some chemicals were investigated on tomato in plastic house from 1997 to 1998. Adults trapped by yellow sticky trap and sweeping net were increased from late May abruptly. Survey of population densities by yellow sticky trap was more clear than sweeping net. Damaged leaves were found out all the year round in continuous cropping fields and rate of damaged leaves was below 20% in early April and increased gradually up to 80% level after late May. Otherwise, damaged leaves were appeared from 6 weeks after transplanting in first growing field and then damaged leaves was 80% level in spring culture. In autumn culture, rate of damaged leaves was maintained 80% level from transplanting to harvesting date regardless of cultivation years. Number of adults was trapped from late May and peak was late June but mature larva was $6{\sim}8$ individuals in spring culture. In autumn culture, adult and larval densities was maintained high for 3 weeks after transplanting but begun to decrease from 4 weeks(early September). Chlorfenapyr EC, cyromazine WP and abamectin EC could suppress larval populations of Liriomyza trifolii effectively.
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