• The Korean Journal of Food And Nutrition
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• v.30 no.4
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• pp.710-718
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• 2017

Contents of water soluble vitamin $B_6$ in a total of 62 agricultural products cultivated in local areas in Korea were analyzed by high performance liquid chromatography with fluorescence detector (HPLC/FLD). To verify the method of vitamin $B_6$ analysis, a quality control chart was formulated with in-house control using a mixture of broccoli and shiitake mushrooms. Among cereals, high content of vitamin $B_6$ measured $234.3{\sim}260.3{\mu}g/100g$ in dried mung bean and soybean. Vitamin $B_6$ content of non-glutinous and glutinous black rice measured $105.0{\mu}g/100g$ and $129.7{\mu}g/100g$, respectively. In vegetables, high content of vitamin $B_6$ were measured in passion fruit ($104.3{\mu}g/100g$), gat ($55.7{\sim}84.3{\mu}g/100g$), gomchwi ($31.3{\sim}88.0{\mu}g/100g$) and garlic ($72.7{\sim}98.3{\mu}g/100g$). Among fruits, gold kiwi 'Zespri' and green kiwi 'Hayward' revealed high vitamin $B_6$ content of $116.3{\mu}g/100g$ and $78.7{\mu}g/100g$, respectively. In persimmons, daebongsi had high vitamin $B_6$ content ($36.0{\sim}72.7{\mu}g/100g$) than bansi and sweet persimmon. Vitamin $B_6$ content in dried jujube and persimmon increased more than $86.7{\mu}g/100g$ compared to fresh materials. Among specialty crops, green tea powder ($64.7{\sim}251.0{\mu}g/100g$) and sansuyu ($172.3{\mu}g/100g$) revealed high content. Of mushrooms, vitamin $B_6$ content of Sparassis crispa ($139.3{\mu}g/100g$) was the highest. Vitamin $B_6$ content information of agricultural products in local areas in Korea collected from this experiment will be used as valuable preliminary data for grasp national nutritional status.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.31-31
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• 2023

Mung bean(Vigna radiata (L.) R. Wilczek var. radiata) is a legume that originated in India. It is the third most cultivated legume in Korea after soybean and adzuki bean. Recently, the use of mung bean seeds and sprouts in trendy foods such as rice noodles and Chinese-style stir-fry is expanding thereby increasing its demand. Subsequently, improvement of mung bean varieties is also being actively conducted. In this study, the important agricultural characteristics of 324 mung bean germplasm were recorded and statistically investigated. Seeds of the mung bean germplasm were cultivated at an experimental field located in the National Agrobiodiversity Center (Jeonju, Korea) and 10 quantitative agricultural traits were investigated. Basic statistics, correlation analysis, and principal component analysis were then performed. The results showed significant variations of the quantitative traits among the germplasms (p < 0.05). The days to flowering, maturity, and growth were in the ranges of 31~80, 22~72, and 57~110 days with means of 45, 47, and 92 days, respectively. The highest frequency (f = 192) was for lodging score with 11~50%, while simultaneous maturity (f = 182) was below 50%. Other quantitative traits related to yield including the number of seeds per pod (CV = 10.9%), number of pods per plant (CV = 41.2%), and one-hundred seeds weight (CV = 36.6%) also showed significant variations. Correlation analysis showed positive correlations between the days to maturity and one-hundred seeds weight (r = 0.41) and the days to growth and simultaneous maturity (r = 0.39). In contrast, one-hundred seeds weight was negatively correlated to the number of pods per plant (r = -0.41) and the days to flowering (r = -0.29). Similarly, the days to growth and the number of pods per plant had a negative association with each other (r = -0.29). The principal component analysis revealed the number of days to maturity as the most influential variable along the first principal component (23.7%). In general, this study revealed wide variations in quantitative traits among the studied mung bean germplasm, which could provide several options for cultivar development.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.58-68
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• 2018

Sunn hemp (Crotalaria juncea) is used as a nitrogen-fixing green manure in Korea to improve soil quality, reduce soil erosion, and suppress weeds and nematodes. In 2014, wilting sunn hemp plants were observed in green manure-cultivated fields in Wanju, Korea. Leaves of the infected plants began yellowing, starting with the lower leaves, eventually leading to their death. Moreover, a number of dark perithecia were observed on the wilting stems. Six isolates were obtained from these perithecia by single spore isolation. Based on their morphological characteristics, the isolates were identified as Fusarium udum (teleomorph: Gibberella indica). Macroconidia were slightly curved with almost hooked apical cell, and microconidia were formed on false heads by monophialides. Chlamydospores were produced abundantly in the hyphae, either singly or in clusters. To confirm the identification, multilocus sequence analysis was conducted using translation elongation factor 1 alpha (TEF), calmodulin (CAL), and histone 3 (HIS3). The sequences of TEF, CAL, and HIS3 showed 94.4~96.2%, 99.7%, and 99.6~99.8% similarity to the reference sequences of F. udum in NCBI GenBank, respectively. Pathogenicity was tested on sunn hemp and two soybean cultivars using the inoculation method of soil drenching with spore suspension. The wilting symptoms were observed only in sunn hemp and one cultivar of soybean (cv. Teagwang) after 14~21 days of inoculation. This is the first report of wilt disease in sunn hemp caused by Fusarium udum in Korea.

• Korean Journal of Breeding Science
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• v.50 no.4
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• pp.442-452
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• 2018

In this study, we analyzed the growth characteristics and isoflavone content of 43 soybean varieties highly adaptable to highland areas. The flowering period of each cultivation zone was from July 15 to August 12 at Daewallyeong, from July 18 to August 11 at Jinbu, and from July 23 to August 13 at Gangneung. The accumulated temperature from flowering to maturity was $1,297^{\circ}C$ for Daegwallyeong, $1,391^{\circ}C$ for Jinbu, and $1,685^{\circ}C$ for Gangneung. Forty-three varieties were classified into four utilities; soy sauce and tofu, bean sprouts, cooking with rice, and vegetable and early maturity. The content of isoflavone was highest at $2,579{\mu}g/g$ in varieties for soy sauce and tofu usage. Five varieties ("Paldalkong," "Sinpaldal2," "Ilmikong," "Sinpaldalkong," and "Daepung") cultivated in Daegwallyeong had over $4,000{\mu}g/g$ of isoflavone. The isoflavone content of the region Daegwallyeong was different at the significance level of 0.1 (p=0.061) compared to Gangneung. There was no significant difference between Gangneung and Jinbu. It is thought that the low temperature of the maturation stage during the growing period affected isoflavone accumulation. The varieties with more than $3,000{\mu}g/g$ of isoflavone content in Daegwallyeong, Jindu, and Gangneung were "L29," "Williams82," "Ilmikong," and "Daepung." These were genetically and environmentally stable in isoflavone content. It is expected that this study will be used as basic data for the functional breeding and selection of soybean varieties highly adaptable to a specific region, and to help expand soybean cultivation areas in highlands.

• Korean Journal of Breeding Science
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• v.51 no.3
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• pp.214-221
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• 2019

'Aram' is a soybean cultivar developed for soy-sprout. It was developed from the crossing of 'Bosug' (Glycine max IT213209) and 'Camp' (G. max IT267356) cultivars in 2007. F₁ plants and F₂ population were developed in 2009 and 2010. A promising line was selected in the F₅ generation in 2011 using the pedigree method and it was evaluated for agronomic traits, yield, and soy-sprouts characteristics in a preliminary yield trial (PYT) in 2012 and an advanced yield trial (AYT) in 2013. Agronomic traits and yield were stable between 2014 and 2016 in the regional yield trial (RYT) in four regions (Suwon, Naju, Dalseong, and Jeju). Morphological characteristics of 'Aram' are as follows: determinate plant type, purple flowers, grey pubescence, yellow pods, and small, yellow, and spherical seeds (9.9 g 100-seeds^-1) with a light brown hilum. The flowering date was the 5^th of August and the maturity date was the 15^th of October. Plant height, first pod height, number of nods, number of branches, and number of pods were 65 cm, 13 cm, 16, 4.5, and 99, respectively. In the sprout test, germination rate and sprout characteristics of 'Aram' were comparable to that of the 'Pungsannamulkong' cultivar. The yield of 'Aram' was 3.59 ton ha^-1 and it was 12% higher than that of 'Pungsannamulkong' in southern area of Korea. The yield of 'Aram' in the Jeju region, which is the main region for soybean sprout production, was 20% higher than that of 'Pungsannamulkong'. The height of the first pod and the tolerance to lodging and pod shattering, which are connected to the adaptation to mechanized harvesting, were higher in 'Aram' compared to those in 'Pungsannamulkong'. Therefore, the 'Aram' cultivar is expected to be broadly cultivated because of its higher soybean sprout quality, and seed yield and better adaptation to mechanized harvesting. (Registration number: 7718)

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.649-656
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• 2002

Fibrinolytic microorganisms were screened from 42 samples of Korean fermented food (7 kinds of Chungook-jang, 14 kinds of commercial Doen-Jang, 5 kinds of home-made Doen-jang, and 16 kinds of Jeot-gal), 15 samples of Japanese fermented food (5 kinds of home-made soybean paste, and 10 kinds of Natto), and 19 samples of Indonesian fermented food (Tempe) as well as starters of Meju (500 microflora from Korea, and 22 from China). Initially, 11 isolates with strong fibrinolytic activity were selected for further characterization. The fibrinolytic activity of the 11 isolates ranged from 89 to 199% of standard plasmin. Four strains, M5l from Korean fermented food (Meju), I 1-1, I 1-4, and I 5-1 from Indonesian fermented food (Tempe), were chosen based on the degree of activity and reproducibility, and identified as Staphylococcus sciuri, Citrobacter or Enterobacter, Enterococcus faecalis, and Bacillus subtilis, respectively. The first two isolates are pathogenic stains while the latter two are considered as GRAS (Generally Recognized As Safe). Fibrinolytic activity of E. faecalis, characterized and designated as BRCA-5, reached a maximum, when the producer was cultivated in Ml7 broth supplemented with 1.0% glucose for 5 h at 37$^{\circ}C$ with shaking at 180 rpm. Compared to commercial fibrinolytic enzymes, the cell-free culture supernatant of 5. faecaiis BRCA-5 showed stronger activity than plasmin and streptokinase, but similar degree of specific activity as nattokinase and urokinase, aud it also demonstrated anticoagulant and antiplatelet activity ex vivo. These features of E. faecalis make it an attractive agent as a biomaterial for health-promoting foods.

• The Korean Journal of Mycology
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• v.18 no.2
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• pp.96-101
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• 1990

This study was conducted to select the promising antifungal microoganisms for biological control of wet bubble, Mycogone perniciosa. Ninty one isolates of fungi, 342 isolates of bacteria, and 556 actinomycetes were isolated from mushroom composts and soils, were subjected to primary screening test on agar medium base for their antimicrobial spectra. Among them, 12 bacteria and 71 actinomycetes were selected. Among the antibiotic producing microoganisms, 5 cultures were selected on the basis their antibiotic activities on casing soil with Benlate nontolerence M. perniciosa. Finally, AJ-117, AJ-136 and AK-139 were selected as microoganism with antifungal activity against two strains of M. perniciosa.

• Microbiology and Biotechnology Letters
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• v.31 no.3
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• pp.271-276
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• 2003

Bacillus amyloliquefaciens D4, which produces a strongly fibrinolytic enzyme, was isolated from Chungkook-Jang, a traditional Korean soybean-fermented food. B. amyloliquefaciens D4 was mutated with N-methyl-N-nitro-N-nitrosoguanidine (MNNG) to yield a series of mutants with increasing levels of fibrinolytic enzyme production. After mutation, a mutant D4-7 was obtained with fibrinolytic activity about eight times stronger than the parent strain. The fibrinolytic activity of B. amyloliquefaciens D4-7, reached a maximum, when the producer was cultivated in 2% Isolated Soy Protein (ISP) broth for 48 h at $37^{\circ}C$. Compared to commercial fibrinolytic enzymes, the cell-free culture supernatant of B. amyloliquefaciens D4-7 showed stronger activity than plasmin and streptokinase. The optimum temperature and pH were $50^{\circ}C$ and 10.0 and thermostability was increased by the addition of glycerol, glucose, and NaCl.

• Microbiology and Biotechnology Letters
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• v.13 no.1
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• pp.13-17
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• 1985

Rhizobium japonicum isolates from all around Korea could be classified into two groups, i.e., acid producing fast-growers with 2.4 hour mean generation time and non-acid producing slow-growers in yeast extract-mannitol medium with 13.1 hour mean generation time. Tested fast-growers were higher in 6-phosphogluconate dehydrogenase activity than slow-growers were and used sucrose as carbon source whereas slow-growers did not. Fast-grower R4, R257, R278, showed tolerance even in 0.5M NaCl or above and the growth of all the strains tested were inhibited at below pH 4.5. Relative symbiotic activities of nitrogen fixation for these isolated with Glycine max cv. Jangyeobkong (commercial soybean cultivar mostly cultivated in Korea) ranged 0.1 to 2.0 comparing to that of R. japonicum L-259 (NRRL), without regard to their growth rate.

• Journal of Internet of Things and Convergence
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• v.6 no.3
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• pp.45-52
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• 2020

In the paper, we propose a system that measures various agricultural parameters that affect crop yield and monitors location information. According to an analysis by international organizations, 60% of the world's population lives on agriculture. In addition, 11% of the world's soil is used for growing crops. For this reason, agriculture plays an important role in national development. If a problem occurs in agriculture due to weather or environmental problems, it can be a problem for national development. In order to solve these problems, it is important to modernize agriculture using modern IoT technology. It is possible to improve the agricultural environment by applying IoT technology in agriculture to build a smart environment. Through such a smart environment, it is possible to increase the yield of agricultural products, reduce water waste, and prevent overuse of fertilizers. In order to verify the proposed system, an experiment was performed in a soybean cultivation farm. Experimental results showed that using the proposed system, the moisture in the cultivated soil can be automatically maintained at 40%.