• 한국지역사회생활과학회지
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• 제17권1호
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• pp.11-21
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• 2006

This research is focused on not only cultivated land as an important factor which represented the agricultural district, but also details of the research in the landscape of cultivated land. Moreover, we also focused on the cultivated land operating environmental friendly agriculture which took a lot of interest among the landscape of the cultivated land. The reason is, we judged environmental friendly agriculture is not only the simple purpose only for manufacturing food but also the key role for preserving their environment and landscape, and making sustainable agriculture. For this reason, our research treats the analysis that operating environmental friendly landscape has what kinds of different factors in landscape characteristic compare with other general agricultural methods. Plus we selected evaluating both the degree of citizen's presence and the degree of rural residents's importance in those factors as a mainly research matter. We drew out the landscape of cultivated land and concerning factors by analyzing policies(ESA, CSS, CTE, MFSP, ACL, etc) concerned with overseas agricultural landscape to deduct landscape Character factors in internal cultivated land. Based on eleven items of landscape factors in cultivated land deducted by documents research, we started to conduct field studies during MAY. 7th. 2004 $\sim$ JUL. 8th, DEC. 1th $\sim$ 3th reaching 32 villages and concerned institutes to deduct the factors in landscape characteristic in agricultural cultivated land in detail. We analyzed degrees of importance and preference in citizen and rural residents classified by 5 province of the whole country to evaluate the landscape Character factors in environmental friendly agriculture deducted by field research and 21 documents including extra items such as a scene of duck grazing by the duck techniques which is very popular way among internal environmental friendly agriculture, gardening space composed around farm and furrow on slope ground formed by unique topology in hilly country. The research period was progressed for 20 days from MAY. 28th. 2005 $\sim$ JUN. 17th. This research is the result that cultivated land landscape as a detail item holding agricultural environment especially, it is also meaningful result in environmental friendly agriculture as a basic research, because it is not only a value of simple food producing but also very effective role in both landscape and environmental parts.

• Journal of Acupuncture Research
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• 제21권6호
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• pp.1-17
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• 2004

Objective : To compare and examine how adjustment of pH and electrolyte can affect the efficacy of cultivated wild ginseng distilled herbal acupuncture, we've administered pure cultivated wild ginseng distilled herbal acupuncture and pH and electrolyte adjusted cultivated wild ginseng distilled herbal acupuncture on A549 human lung cancer lines. Then mRNA and proteins which take parts in apoptosis were examined. Methods : Pure cultivated wild ginseng distilled herbal acupuncture treated group was set as the control group and pH and electrolyte adjusted cultivated wild ginseng distilled herbal acupuncture groups were administered on A549 human lung cancer lines. Cell toxicity was carefully examined and from the analysis of DNA fragmentation, RT-PCR, and Western blot, manifestation of mRNA and proteins which are associated with apoptosis were inspected. Results : The following results were obtained on apoptosis of A549 human lung cancer lines after administering pH and electrolyte adjusted cultivated wild ginseng distilled herbal acupuncture. 1. Measuring cell toxicity of lung cancer cells, higher cell toxicity was detected at pH and electrolyte adjusted groups and the results were concentration dependent. 2. Through DNA fragmentation, we were able to confirm cell destruction in all groups. 3. Experiment groups treated with cultivated wild ginseng distilled herbal acupuncture showed inhibition of Bcl-2 and COX-2 at mRNA and Protein level, whileas increase of Bax was shown. 4. Manifestation of p21, p53, Cyclin E, and Cyclin D1 were confirmed in all groups. 5. Extrication of Cytochrome C was detected at all groups, as well as increased activity of the enzyme caspase-3 and caspase-9, and PARP fragmentation were confirmed. Conclusions : From the above results, we can carefully deduce cell destruction of A549 human lung cancer lines were induced by Apoptosis. At the same concentration level, cell destruction efficacy was better with adjusted pH and electrolyte. Cultivated wild ginseng distilled herbal acupuncture also showed decrease of Bcl-2 and COX-2, as well as increase of Bax. Since cultivated wild ginseng distilled herbal acupuncture increases manifestation of p21, p53, Cyclin E, and Cyclin D1, it affects cellular cycle and through these phenomena, we can consider extrication of Cytochrome C, increase of caspase, and PARP fragmentation are the results.

• 한국식품저장유통학회지
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• 제19권3호
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• pp.428-432
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• 2012

본 실험은 탄산수를 재배용수로 사용하였을 때 탄산수가 콩나물의 생육특성인 수량, 개체 중량, 길이 및 두께와 비타민 C, isoflavone과 같은 유용성분에 미치는 영향을 조사하기 위해 수행하였다. 제주산 준저리 품종의 콩 100 g을 불린 후 $22{\pm}1^{\circ}$에서 탄산수(pH 4.6)와 음용수(pH 7.8)로 6일간 재배하였다. 탄산수로 6일간 재배한 콩나물의 수율은 255.1 g으로 음용수로 재배한 대조구의 176.1 g에 비해 약 1.45배 높게 나타났으며, 중량은 $0.91{\pm}0.205$ g으로 1.3배 높게 나타났다. 길이도 $15.11{\pm}1.592$ cm로 대조구의 $12.92{\pm}1.914$ cm에 비해 1.2배 높았다. 콩나물의 두께는 탄산수로 재배한 경우와 대조구가 유사하였다. 탄산수로 재배한 콩나물의 비타민 C 함량은 1.13 mg% 로 대조구에 비해 약 2배 정도 높게 나타났다. 탄산수로 재배한 경우 genistein이 $11.7{\pm}0.14{\mu}g/g$, daidzein이 $74.7{\pm}0.35{\mu}g/g$로 대조구의 genistein과 daidzein함량이 각각 $8.4{\pm}0.08{\mu}g/g$와 $64.9{\pm}0.05{\mu}g/g$ 보다 높게 나타났다. Daidzein과 genistein의 함량비율은 탄산수로 재배한 경우 6.4인데 반해 대조구는 7.7로 다소 높게 나타났다. 이와 같이 탄산수로 재배한 콩나물에서 수량과 길이, 중량, 두께 등 생장이 효율적이고 비타민 C, isoflavone 같은 유효성분 함량이 대조구에 비해 높게 나타났다.

• 환경생물
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• 제21권3호
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• pp.271-275
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• 2003

유기농업을 수행해온 배추와 무의 근권토양과 관행농법의 근권토양 및 비경작지 토양으로부터 토양시료를 채취하여 선충의 밀도를 조사하고, 토양내 유익선충인 곤충병원성 선충의 분포상황을 검토하였다. 경작지 토양 내 선충 분포율이 비경작지에 비하여 높았고, 유기농업 토양이 관행농업 토양보다 선충의 밀도가 높게 나타났다. 선충의 정량적 평가법으로 직접계수법과 MPN 법을 비교 조사한 결과 MPN법이 신속하고 정확한 방법임이 확인되었다. 각 토양으로부터 분리된 곤충병원성 선충은 Rhabditida목 선충과 Diplogasterida목 선충으로 분류되었다. 이들 선충 중 Diplogasterida목 선충이 50％ 이상을 차지하여 높은 점유율을 나타내었다. 누에를 기주체로 사용한 살충력 검정에 있어 24∼36시간 이내에 90％이상의 기주 치사율을 보여 농작물 해충방제를 위한 생물농약으로의 활용 가능성을 나타내었다.

• Journal of Ginseng Research
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• 제40권2호
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• pp.113-120
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• 2016

Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

• 한국환경과학회지
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• 제21권5호
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• pp.641-646
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• 2012

Available phosphorus($P_2O_5$) in conventionally cultivated soil was more abundant in two fold than that of organically cultivated soil. Relative density of Arbuscular Mycorrhizal Fungi (AMF) was higher in organically cultivated soil, That of welsh onion cultivated soil was the highest, that of strawberry was followed and then that of pepper, respectively. Relative density of AMF was inversely proportioned to available soil phosphorus. Phosphorus content of crop and relative density of AMF were more abundant in organically cultivated crop or soil. However available soil phosphorus content was much in conventionally cultivated soil. The phosphorus contents between soil and crop were negatively correlated. The phosphorus content of crop was increased as the relative density of AMF increased. Relative density of AMF in the organically cultivated soil and phosphorus content of the crop with organic cultivation were higher than those of conventionally cultivated.

• 한국초지조사료학회지
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• 제16권4호
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• pp.283-290
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• 1996

This experiment was carried out to determine the effects of soil condition that previous crop cultivated after [TO: Soil of corn cultivated, T1 : Soil of rye monoculture cultivated(l50kg/ha), T2 : Soil of mixedculture cultivated(Rye : Red clover = 120 : 3koa). T3 : Soil of mixed-culture cultivated(Rye : Red clover = 90 : 6kg/ ha), T4 : Soil of mixedculture cultivated(Rye : Red clover = 60 : 9kg/ha), T5 : Soil of mixedculture cultivated (Rye: Red clover = 30 : 12kg/ha), T6 : Soil of red clover monoculture cultivated(l5kgha)) on the growth characteristics and nutrient yield of silage corn. The results are summarized as follows. 1. The plant length of silage corn was high in order of T6>T5>T4 treatment. In the leaf length, T5 and T6 treatment showed short compared to the other treatments, but they were wider than the other treatments in leaf width. In the number of leaf, T6 treatment was the highest as 14 leaves per plant, while T1 treatment showed lower than the other treatments as 12.6 leaves per plant. 2. In the stem hardness, ear length, ear width and ear full degree, soil treatment of red clover mono-culture cultivation(T6) showed higher than the other treatment as 5.1 1kg/$cm^2$, 20.8~~1, 7.7cm and 97%. respectively (P<0.01, 0.05). Ihe leaf of summer depression did not many occurrence, but T6 treatment was lower than the other treatment as 0.8 leaf per plant. 3. ADF and NDF composition were not affected by soil condition. Crude protein composition of T6 treatment was the highest as 7.8%. while TI treatment was the lowest as 6.9%. Fresh weight yield(59,083kg/ha), dry matter yield(21,743kg/ha), crude protein yield(l,369kg/ha) and TDN yield(15,800kg/ha) at T6 treatment were much more increased by 41.9, 47.5, 57.4 and 49% than TI treatment(39,410, 14,259, 827 and 10,056kg/ha).

• 한국조경학회지
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• 제22권4호
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• pp.95-117
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• 1995

This study was carried out to investigate Korean native trees in the America landscape through arboretums, the plant inventory of America, computer files for accessions from Korea, source lists of plants and seeds, and seed and plant nursery company. The results were as follows; 1. Korean native trees introduced in America and Canada were 119 species, 116 species were being cultivated in arbrids were being sold in nurseries, new cultivars were selected from 32 species, and hybrids were bred from 5 species. 2. Abies koreana, Malus baccata, and Stewartia koreana were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids as well as new cultivars. 3. 25 species including Albizzia julibrissin were being cultivated in many arboretums and sold in many nurseries. Also there were new hybrids. 4. Ilex integra and Ilex rotunda were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars, but there were new hybrids. 5. 44 species including Abies holophylla were being cultivated in many arboretums and sold in many nurseries. There were no new cultivars or hybrids. 6. Populus tremula var. davidiana was being cultivated in many arboretums. There were new cultivars of 'Erecta', 'Gigas', 'Pendula', but no new hybrids. They were not sold by any nurseries. 7. 41 species including Abies nephrolepsis were being cultivated in many arboretums. But they were not sold by any nurseries. They didn't have any new cultivars or hybrids. 8. Diospyros kaki 'Kyungsan Bansi', Elaeocarpus sylvestris var. ellipticus, and Pyrus serotina 'Dankae' were not being cultivated in arboretums in America but some nurseries were selling those species as well as new cultivars. 9. Only 3 species (Berchemia berchemiaefolia, Carpinus eximia, and Chionanthus retusus var. coreana which are native Korean plants) were not introduced in America or Canada.

• 한국식품영양학회지
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• 제8권4호
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• pp.301-306
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• 1995

This study was carried out to find out rice quality of Chucheongbyeo cultivated on Gyeonggj and other province. Rice type, color difference, amylograph of milled rice and texturograph of cooked rice were not significant between two cultivated region. And fat, protein, Mg and Ca in milled rice were not significant but p, K and Na were significantly different at l% the level between them as 2, 210~2, 420ppwt 1, 304~1, 538ppm and 212~236ppm in Gyeonggi province cultivated rice, and 2, 130~2, 180ppm, 1, 133~1, 180ppm and 197~217ppm in other province, respectively. Also amylose content, alkali distribution and gel consistency were not significant between them.

• 대한약침학회지
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• 제13권1호
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• pp.37-43
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• 2010

Objective : Ginseng is one of most widely used herbal medicine. Ginseng showed anti-metastasis activities. However, its molecular mechanisms of action are unknown. So we want to report the wild ginseng repress which plays key roles in neoplastic epithelial-mesenchymal transition process. Methods : Treatment of the human colorectal carcinoma LOVO cells and human gastric carcinoma SNU601 cells with the increased concentrations of cultivated wild ginseng extracts resulted in a gradual decrease in the AXIN2 gene expression. Results : Metastasis-suppressor genes, maspin and nm23 was not affected by the treatment of ginseng extracts in LOVO cells. Moreover, the mountain cultivated wild ginseng or mountain wild ginseng are similar in their inhibitory effects on the expression of AXIN2 gene, but are substantially stronger than cultivated ginseng. Conclusion : We described the novel mechanism of wild ginseng-induced anti-metastasis activity by repressing the expression of AXIN2 gene that plays key roles in epithelial-mesenchymal transition process.