• Journal of Soil and Groundwater Environment
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• v.8 no.4
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• pp.53-63
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• 2003

Objectives of this research were to fractionate heavy metals in soil samples in the upper Okdong River basin and to assess the potential pollution index of each metal fraction. Soil samples were collected from the cultivated land soils and analyzed for physical and chemical properties. pH of cultivated soils ranged from 5.2 to 7.6. Contents of total kelhaldal nitrogen and loss on ignition were in the ranges of 0.6∼2.5%, and 1.9∼12.9%, respectively. Heavy metals in the cultivated land soils were higher in the abandoned closed coal mine near field soils than those in the paddy soils. Total concentrations of metals in the cultivated land soils were in the orders of Zn > Pb > Ni > Cu > Cd, exceed the corrective action level of the Soil Environment Conservation Law and higher than the naturals were abundance levels reported from uncontaminated cultivated land soils. Mobile fractions of metals were relatively small compared to the total concentrations. Soil Pollution Assesment Index (SPAI) values of each fraction of metals were leveled from Non polluted to Moderately polluted based on total concentrations. SPAI values of mobil fractions were lower than those of immobile fractions. Results on metal fractions and SPAI values of the cultivated land soils indicate that field soils samples were contaminated with heavy metals and had potential to cause a detrimental effects on plants. A prompt countermeasure to prevent field soils in the abandoned closed coal mine near fields are urgently needed.

• Journal of the Korean Society of Food Culture
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• v.14 no.5
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• pp.529-534
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• 1999

Amino acids, minerals and color of Codonopsis lanceolota that is one of Korean indigenous culinary herbs were analyzed. Arginine, glutamine, alanine and proline were the major total amino acids in wild, organic cultivated and cultivated Codonopsis lanceolata. The content of arginine was the highest free amino acid in wild, organic cultivated and cultivated Codonopsis lanceolata. The contents of arginine and K were the highest in organic cultivated Codonopsis lanceolata that was obtained by rice straw and fallen leaves application. Wild Codonopsis lanceolata was containing $3{\sim}5$ times Ca than organic cultivated and cultivated one. The contents of Pb and Cd were lower than 'countermeasure values for foodstuffs contamination'. Values of a, b and ${\Delta}E$ were significantly high in wild Codonopsis lanceolata than cultivated one.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.48-56
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• 2018

The present study aimed to understand the variation in seed germination according to the storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea. In this milieu, we investigated the germination rate and germination energy of 59 Perilla accessions (15 cultivated var. frutescens type I, 4 cultivated var. frutescens type II, 20 weedy var. frutescens, and 20 weedy var. crispa), which were harvested in autumn 2016. The accessions of cultivated var. frutescens (type I) showed an average germination rate of 80.8% 6 months after harvest; however, the accessions of weedy var. frutescens and var. crispa showed an average germination rate of 0.9% and 8.7%, respectively. Furthermore, the accessions of cultivated var. frutescens (type II) showed an average germination rate of 18.2%. The accessions of cultivated var. frutescens (type I) showed an average germination energy of 75.8%, while the accessions of weedy var. frutescens and var. crispa showed an average germination energy of 0.6% and 6.9%, respectively. In addition, the accessions of cultivated var. frutescens (type II) showed an average germination energy of 14.3%. The germination rate and germination energy for the accessions of cultivated and weedy types of Perilla species increased marginally at 2 months from the first month after harvest. However, it did not significantly increase until six months after that. According to our results, there are two types of cultivated var. frutescens, namely, type I, which showed high germination rate and germination energy, and type II, which showed low germination rate and germination energy. The results of this study will provide basic information to understand variations in the germination of seeds during 6 months of storage period after harvest in the accessions of cultivated and weedy types of Perilla species in Korea.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.9
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• pp.1351-1356
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• 2013

To enhance the utilization of Allium hookeri (AH) as a food, characteristics of AH roots and leaves cultivated under open field and greenhouse conditions were investigated. The moisture content of the roots and leaves were 81.05 to 84.18% and 88.85 to 90.12%, respectively. The moisture content of AH cultivated in the open field was 2 to 3% lower than the moisture content of AH cultivated in the greenhouse for both roots and leaves. The content of nitrogen-free extract, carbohydrates, was 13.49 to 16.20% in the roots and 7.08 to 7.79% in the leaves. The main mineral generated from both open field and greenhouse cultivation was potassium, at 503.98 to 512.08 mg% in leaves. The free sugar content of roots cultivated in the open field was four times higher than the content in the leaves, and roots cultivated in the greenhouse contained three times lower free sugar than the leaves. In particular, the fructose content of roots cultivated in the open field was about 12 times higher than roots cultivated in the greenhouse. The crude saponin and total polyphenol content was higher in leaves than roots, and was higher in the open field than the greenhouse. The $IC_{50}$ for DPPH radical scavenging activity was highest, 2.74 mg/mL, in 70% MeOH extracts of AH leaves cultivated in the greenhouse. Water and 70% MeOH extracts of AH leaves cultivated in the greenhouse showed no cytotoxicity to RAW 264.7 cells. Water extracts of AH leaves cultivated in the open field markedly inhibited the production of the inflammatory mediator nitric oxide. These results suggest that AH may be used as the material of health functional food.

• 경락경혈학회:학술대회논문집
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• 2006.11a
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• pp.137-137
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• 2006

• Development and Reproduction
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• v.16 no.4
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• pp.339-351
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• 2012

Fetal bovine serum (FBS) is the most frequently used serum for the cultivation of mammalian cells. However, since animal-derived materials might not be appropriate due to safety issues, allogeneic human serum (HS) has been used to replace FBS, particularly for the culture of human cells. While there has been a debate about the advantages of HS, its precise effect on human adult stem cells have not been clarified. The present study aimed to investigate the effect of HS on the human eyelid adipose stem cells (HEACs) in vitro. When HEACs were cultivated in a medium containing 10% HS, many cells moved into several spots and aggregated there. The phenomenon was observed as early as 9 days following 10% HS treatment, and 12 days following 5% HS plus 5% FBS treatment. However, the aggregation was never observed when the same cells were cultivated with 10% FBS or bovine serum albumin. To examine whether cell density might affect the aggregation, cells were seeded with different densities on 12-well dish. Until the beginning of aggregation, cells seeded at low densities exhibited the longest culture period of 16 days whereas cells seeded at high densities showed the shortest period of 9 days to form aggregation. The number of cells was $15.1{\pm}0.2{\times}10^4$ as the least for the low density group, and $29.3{\pm}2.8{\times}10^4$ as the greatest for the high density group. When human cord blood serum or normal bovine serum was examined for the same effect on HEACs, interestingly, cord blood serum induced the aggregation of cells whereas bovine serum treatment has never induced. When cells were cultivated with 10% HS for 9 days, they were obtained and analyzed by RT-PCR. Compared to FBS-cultivated HEACs, HS-cultivated HEACs did not express VIM, and less expressed GATA4, PALLD. On the other hand, HS-cultivated HEACs expressed MAP2 more than FBS-cultivated HEACs. In conclusion, human adult stem cells could move and form aggregates by the treatment with human body fluids.

• Korean Journal of Medicinal Crop Science
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• v.11 no.3
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• pp.216-223
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• 2003

This experiment was carried out to investigation the growth differentiation between direct sowing and transplanting on P. ginseng cultivated on field and underwood. The results were as follows: In direct sowing treatment, emergence rate of P. ginseng cultivated on field and under conifer forest was above 80%, but it was only 40% at cultivated under broad-leaved forest. And survival rate was directly diminished about 10% every year. In transplanting treatment, survival rate was also diminished every year, it was slighter than that of direct sowing treatment. The growth amount of P. ginseng cultivated underwood was poor, compared to field culture, but there was no significant between conifer forest and broad-leaved forest. In transplanting treatment of 1 to 6 year-root seedling, survival rate of P. ginseng cultivated underwood was equal or slightly good compared to that of cultivated on field. Among seedling age, survival rate of elder seedling was lower than that of younger seedling. In P. ginseng cultivated underwood, the annual growth increment of 1 and 2 year-root seedling increased slowly and continually, whereas, that of 3 to 6 year-root seedling stopped or decreased slightly.

• Korean Journal of Soil Science and Fertilizer
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• v.27 no.3
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• pp.215-219
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• 1994

This experiment was tried for finding out some soil chemical problems when new apple trees were replanted in old orchards. Soil samples were collected from the soil horizons in the old apple orchards cultivated over 40 years and reference soils. The non-cultivated reference soils were located near the old apple orchards and each of the soils was showed as the same pedon with each of the cultivated soils. The results were as follows : Soil pH showed a tendency to decrease in low horizons of the cultivated soils whereas increase in those of the uncultivated soils. As a comparision with each chemical component, the content of exchangeable Ca or total Mn was likely to be deficient in the cultivated soils. But all components except those were not like that. Total exchangeable cations in the cultivated soils were lower than in uncultivated soils. The pH in the cultivated soils showed very high positive correlation with total exchangeable cations. From those result, it was assumed that lower pH in lower horizon which would be originated from low content of total exchangeable cations, reacts as a factor for the deterioration of old apple orchard soil.

• Journal of Pharmacopuncture
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• v.6 no.2
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• pp.7-27
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• 2003

Objective : The purpose of this study was to investigate acute and subacute toxicity and sarcoma-180 anti-cancer effects of herbal acupuncture with cultivated wild ginseng (distilled) in mice and rats. Method : Balb/c mice were injected intravenous with cultivated wild ginseng herbal acupuncture for $LD_{50}$ and acute toxicity test. Sprague-Dawley rats were injected intravenous with cultivated wild ginseng herbal acupuncture for subacute toxicity test. The cultivated wild ginseng herbal-acupuncture was injected at the tail vein of mice. Results : 1. In acute $LD_{50}$ toxicity test, there was no mortality thus unable to attain the value. 2. Examining the toxic response in the acute toxicity test, there was no sign of toxication. 3. In acute toxic test, running biochemical serum test couldn't yield any differences between the control and experiment groups. 4. In subacute toxicity test, there was no sign of toxication in the experimental groups and didn't show any changes in weight compared to the normal group. 5. In subacute toxicity test, biochemical serum test showed significant increase of Total albumin, Albumin, and Glucose in the experimental group I compared with the control group. Significant decrease of GOT, ALP, GPT, and Triglyceride were shown. In experiment group II, only Glucose showed significant increase compared with the control group. 6. Measuring survival rate for anti-cancer effects of Sarcoma-180 cancer cell line, all the experimental groups showed significant increase in survival rate. 7. Measuring NK cell activity rate, no significant difference was shown throughout the groups. 8. Measuring Interleukin-2 productivity rate, all the experimental groups didn't show significant difference. 9. For manifestation of cytokine mRNA, significant decrease of interleukin-10 was witnessed in the experimental group compared to the control group. Conclusion : According to the results, we can conclude cultivated wild ginseng herbal acupuncture caused negligible toxicity, and had anti-tumor effects in mice.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.623-634
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• 2019

Little known the connections between soybeans mitogen-activated protein kinase (MAPK) gene expression and the rhizomicrobiome upon invasion of the root pathogen Fusarium oxysporum. To address this lack of knowledge, we assessed the rhizomicrobiome and root transcriptome sequencing of wild and cultivated soybean during the invasion of F. oxysporum. Results indicated F. oxysporum infection enriched Bradyrhizobium spp. and Glomus spp. and induced the expression of more MAPKs in the wild soybean than cultivated soybean. MAPK gene expression was positively correlated with Pseudomonadaceae but negatively correlated with Sphingomonadaceae and Glomeraceae in both cultivated and wild soybean. Specifically, correlation profiles revealed that Pseudomonadaceae was especially correlated with the induced expression of GmMAKKK13-2 (Glyma.14G195300) and GmMAPK3-2 (Glyma.12G073000) in wild and cultivated soybean during F. oxysporum invasion. Main fungal group Glomeraceae was positively correlated with GmMAPKKK14-1 (Glyma.18G060900) and negatively correlated with GmRaf6-4 (Glyma.02G215300) in the wild soybean response to pathogen infection; while there were positive correlations between Hypocreaceae and GmMAPK3-2 (Glyma.12G073000) and between Glomeraceae and GmRaf49-3 (Glyma.06G055300) in the wild soybean response, these correlations were strongly negative in the response of cultivated soybean to F. oxysporum. Taken together, MAPKs correlated with different rhizomicrobiomes indicating the host plant modulated by the host self-immune systems in response to F. oxysporum.