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http://dx.doi.org/10.3746/jkfn.2013.42.9.1351

Chemical Components, DPPH Radical Scavenging Activity and Inhibitory Effects on Nitric Oxide Production in Allium hookeri Cultivated under Open Field and Greenhouse Conditions  

Won, Jun-Yeon (Dept. of Oriental Health Care, J oongbu University)
Yoo, Young-Choon (Dept. of Microbiology, College of Medicine, Konyang University)
Kang, Eun-Ju (Dept. of Microbiology, College of Medicine, Konyang University)
Yang, Hye (Dept. of Microbiology, College of Medicine, Konyang University)
Kim, Gwan-Hou (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Seong, Bong-Jae (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Kim, Sun-Ick (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Han, Seung-Ho (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Lee, Sox-Su (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Lee, Ka-Soon (Geumsan Ginseng & Medicinal Crop Experiment Station, CNARES)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.42, no.9, 2013 , pp. 1351-1356 More about this Journal
Abstract
To enhance the utilization of Allium hookeri (AH) as a food, characteristics of AH roots and leaves cultivated under open field and greenhouse conditions were investigated. The moisture content of the roots and leaves were 81.05 to 84.18% and 88.85 to 90.12%, respectively. The moisture content of AH cultivated in the open field was 2 to 3% lower than the moisture content of AH cultivated in the greenhouse for both roots and leaves. The content of nitrogen-free extract, carbohydrates, was 13.49 to 16.20% in the roots and 7.08 to 7.79% in the leaves. The main mineral generated from both open field and greenhouse cultivation was potassium, at 503.98 to 512.08 mg% in leaves. The free sugar content of roots cultivated in the open field was four times higher than the content in the leaves, and roots cultivated in the greenhouse contained three times lower free sugar than the leaves. In particular, the fructose content of roots cultivated in the open field was about 12 times higher than roots cultivated in the greenhouse. The crude saponin and total polyphenol content was higher in leaves than roots, and was higher in the open field than the greenhouse. The $IC_{50}$ for DPPH radical scavenging activity was highest, 2.74 mg/mL, in 70% MeOH extracts of AH leaves cultivated in the greenhouse. Water and 70% MeOH extracts of AH leaves cultivated in the greenhouse showed no cytotoxicity to RAW 264.7 cells. Water extracts of AH leaves cultivated in the open field markedly inhibited the production of the inflammatory mediator nitric oxide. These results suggest that AH may be used as the material of health functional food.
Keywords
Allium hookeri (AH); general component; DPPH radical scavenging activity; cytotoxicity;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Kim CH, Lee MA, Kim TW, Jang JY, Kim HJ. 2012. Antiinflammatory effect of Allium hookeri root methanol extract in LPS-induced RAW264.7 cells. J Korean Soc Food Sci Nutr 41: 1645-1648.   DOI   ScienceOn
2 Bae GC, Bae DY. 2012. The anti-inflammatory effects of ethanol extract of Allium Hookeri cultivated in South Korea. Kor J Herbology 27: 55-61.   과학기술학회마을   DOI   ScienceOn
3 Lee SM, Lee SI. 2013. Seasoning composition with Allium hookeri for manufacturing kimchi, method of preparing the same and kimchi having the same. Korean Patent 1012946540000.
4 Jeon ES. 2012. Manufacturing method of Allium hookeri jangajji. Korean Patent 1012590370000.
5 Jeon ES. 2013. Manufacturing method of Allium hookeri powder for meat sauce, meat sauce made from the Allium hookeri powder and seasoned meat made from the meat sauce. Korean Patent 1012226620000.
6 Kwak YJ, Chun HJ, Kim JS. 1998. Chlorophyll, mineral contents and SOD-like activities of leeks harvested at different times. Korean J Soc Food Sci 14: 513-515.   과학기술학회마을
7 Hong JH, Lee MH, Kang MC, Hur SH. 2000. Separation and identification of antimicrobial compounds from Korean leek (Allium tuberosum). J Fd Hyg Safety 15: 235-240.   과학기술학회마을
8 Kim KH, Kim HJ, Byun MW, Yook HS. 2012. Antioxidant and antimicrobial activities of ethanol extract from six vegetables containing different sulfur compounds. J Korean Soc Food Sci Nutr 41: 577-583.   과학기술학회마을   DOI   ScienceOn
9 AOAC. Official methods of analysis. 1995. 16th ed. Association of Official Analytical Chemists, Washington, DC, USA. p 69-74.
10 Ando T, Tanaka O, Shibata S. 1971. Chemical studies on the oriental plant drugs (XXV). Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Soyakugaku Zasshi 25: 28-33.
11 Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J Biol Chem 12: 239-243.
12 Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200.   DOI   ScienceOn
13 Bredt DS, Snyder SH. 1994. Nitric oxide: a physiologic messenger molecule. Annu Rev Biochem 63: 175-195.   DOI   ScienceOn
14 Harris SG, Padilla J, Koumas L, Ray D, Phipps RP. 2002. Prostaglandins as modulators of immunity. Trends Immunol 23: 144-150.   DOI   ScienceOn
15 National Rural Living Science Institute. 2011. Food Composition Table 8th Revision. RDA, Suwon, Korea. Vol II, p 152-154.
16 Nam KY. 1996. The new Korean ginseng (constituent and its pharmacological efficacy). Korea Ginseng & Tabacco Research Institute, Daejeon, Korea. p 13-23.
17 Ide N, Lau BHS. 2001. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear factor-${\kappa}B$ activation. J Nutr 131: 1020S-1026S.
18 Kleemann R, Verschuren L, Morrison M, Zadelaar S, van Erk MJ, Wielinga PY, Kooistra T. 2011 Antiinflammatory, anti-proliferative and anti-atherosclerotic effects of quercetin in human in vitro and in vivo models. Atherosclerosis 218: 44-52.   DOI   ScienceOn
19 Rivera L, Moron R, Sanchez M, Zarzuelo A, Galisteo M. 2008. Quercetin ameliorates metabolic syndrome and improves the inflammatory status in obese Zucker rats. Obesity (Silver Spring) 16: 2081-2087.   DOI   ScienceOn
20 Nathan C. 1992. Nitric oxide as a secretory product of mammalian cells. FASEB J 6: 3051-3064.
21 http://fse.foodnara.go.kr/origin/search_content_detail.jsp?idx=9585&query=삼채.
22 Ayam VS. 2011. Allium hookeri, Thw. Enum. A lesser known terrestrial perennial herb used as food and its ethnobotanical relevance in Manipur. Afr J Food Agric Nutr Dev 11: 5389-5412.