Browse > Article

Aggregation of Human Eyelid Adipose-derived Stem Cells by Human Body Fluids  

Song, Yeonhwa (Dept. of Biotechnology, Seoul Women's University)
Yun, Sujin (Dept. of Biotechnology, Seoul Women's University)
Yang, Hye Jin (Dept. of Biotechnology, Seoul Women's University)
Yoon, A Young (Dept. of Biotechnology, Seoul Women's University)
Kim, Haekwon (Dept. of Biotechnology, Seoul Women's University)
Publication Information
Development and Reproduction / v.16, no.4, 2012 , pp. 339-351 More about this Journal
Abstract
Fetal bovine serum (FBS) is the most frequently used serum for the cultivation of mammalian cells. However, since animal-derived materials might not be appropriate due to safety issues, allogeneic human serum (HS) has been used to replace FBS, particularly for the culture of human cells. While there has been a debate about the advantages of HS, its precise effect on human adult stem cells have not been clarified. The present study aimed to investigate the effect of HS on the human eyelid adipose stem cells (HEACs) in vitro. When HEACs were cultivated in a medium containing 10% HS, many cells moved into several spots and aggregated there. The phenomenon was observed as early as 9 days following 10% HS treatment, and 12 days following 5% HS plus 5% FBS treatment. However, the aggregation was never observed when the same cells were cultivated with 10% FBS or bovine serum albumin. To examine whether cell density might affect the aggregation, cells were seeded with different densities on 12-well dish. Until the beginning of aggregation, cells seeded at low densities exhibited the longest culture period of 16 days whereas cells seeded at high densities showed the shortest period of 9 days to form aggregation. The number of cells was $15.1{\pm}0.2{\times}10^4$ as the least for the low density group, and $29.3{\pm}2.8{\times}10^4$ as the greatest for the high density group. When human cord blood serum or normal bovine serum was examined for the same effect on HEACs, interestingly, cord blood serum induced the aggregation of cells whereas bovine serum treatment has never induced. When cells were cultivated with 10% HS for 9 days, they were obtained and analyzed by RT-PCR. Compared to FBS-cultivated HEACs, HS-cultivated HEACs did not express VIM, and less expressed GATA4, PALLD. On the other hand, HS-cultivated HEACs expressed MAP2 more than FBS-cultivated HEACs. In conclusion, human adult stem cells could move and form aggregates by the treatment with human body fluids.
Keywords
Aggregation; Human eyelid adipose-derived stem cells; Human allogeneic serum; Human body fluids;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Assmus B, Schachinger V, Teupe C, Britten M, Lehmann R, Dobert N, Grunwald F, Aicher A, Urbich C, Martin H, Hoelzer D, Dimmeler S, Zeiher AM (2002) Transplantation of progenitor cells and regereration enhancement in acutemycardial infarction. Circulation 106:3009-3017.   DOI   ScienceOn
2 Bieback K, Hecker A, Kocaomer A, Lannert H, Schallmoser K, Strunk D, Kluter H (2009) Human alternatives to fetal bovine serum for the expansion of mesenchymal stromal cells from bone marrow. Stem Cells 27:2331-2341.   DOI   ScienceOn
3 Bogoch Y, Linial M (2007) Coordinated expression of cytoskeleton regulating genes in the accelerated neurite outgrowth of P19 embryonic carcinoma cells. Exp Cell Res 314:677-690.
4 Burridge PW, Thompson S, Millrod MA, Weinberg S, Yuan X, Peters A, Mahairaki V, Koliatsos VE, Tung L, Zambidis ET (2011) A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability. PLoS One 6:e18293.   DOI   ScienceOn
5 Chang L, Goldman RD (2004) Intermediate filaments mediate cytoskeletal crosstalk. Nat Rev Mol Cell Biol 5:601-613.   DOI   ScienceOn
6 Chin YR, Toker A (2010) The actin-bunding protein palladin is an Akt1-specific substrate that regulates breast cancer cell migration. Mol Cell 38:333-344.   DOI   ScienceOn
7 D'Amour KA, Bang AG, Eliazer S, Kelly OG, Aqulnick AD, Smart NG, Moorman MA, Kroon E, Carpenter MK, Baetge EE (2006) Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol 24:1392-1401.   DOI   ScienceOn
8 Erickson GA, Bolin SR, Landgraf JG (1991) Viral contamination of fetal bovine serum used for tissue culture: risks and concerns. Dev Biol Stand 75:173-175.
9 Haack-Sorensen M, Hansen SK, Hansen L, Gaster M, Hyttel P, Ekblond A, Kastrup J (2012) Mesenchymal stromal cell phenotype is not influenced by confluence during culture expansion. Stem Cell Rev May 30. [Epub ahead of print]
10 Hatlapatka T, Moretti P, Lavrentieva A, Hass R, Marquardt N, Jacobs R, Kasper C (2011) Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions. Tissue Eng Part C Methods 17:485-493.   DOI   ScienceOn
11 Horwitz EM, Prockop DJ, Fitzpatrick LA, Koo WW, Gordon PL, Neel M, Sussman M, Orchard P, Marx JC, Pyeritz RE, Brenner MK (1999) Transplantability and therapeutic effects of bone marrow-derived mesenchymal cells in children with osteogenesis imperfecta. Nat Med 5:309-313.   DOI   ScienceOn
12 Jung J, Moon N, Ahn JY, Oh EJ, Kim M, Cho CS, Shin JC, Oh IH (2009) Mesenchymal stromal cells expanded in human allogenic cord blood serum display higher self-renewal and enhanced osteogenic potential. Stem Cells Dev 18:559-571.   DOI   ScienceOn
13 Kang HM, Kim J, Park S, Kim J, Kim H, Kim KS, Kim EJ, Seo SI, Kang SG, Lee JE, Lim H (2009) Insulin-secreting cells from human eyelid-derived stem cells alleviate type I diabetes in immunocompetent mice. Stem Cells 27:1999-2008.   DOI   ScienceOn
14 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomason JA (2001) Hematopoietic colony-forming cells derived from human embryonic stem cells. Proc Natl Acad Sci USA 98:10716-10721.   DOI   ScienceOn
15 Kocaoemer A, Kern S, Kluter H, Bieback K (2007) Human AB serum and thrombin-activated plateletrich plasma are suitable alternatives to fetal calf serum for the expansion of mesenchymal stem cells from adipose tissue. Stem Cells 25:1270-1278.   DOI   ScienceOn
16 Majmundar AJ, Wong WJ, Simon CM (2010) Hypoxia inducible factors and the response to hypoxic stress. Mol Cell 40:294-309.   DOI   ScienceOn
17 Kroon E, Martinson LA, Kadoya K, Bang AG, Kelly OG, Eliazer S, Young H, Richardson M, Smart NG, Cunningham J, Agulnick AD, D'Amour KA, Carpenter MK, Baetge EE (2008) Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol 26:443-452.   DOI   ScienceOn
18 Le Blanc K, Samuelsson H, Lonnies L, Sundin M, Ringden O (2007) Generation of immunosuppressive mesenchymal stem cells in allogeneic human serum. Transplantation 84:1055-1059.   DOI   ScienceOn
19 Ma HY, Yao L, Yu YQ, Li L, Ma L, Wei WJ, Lu XM, Du LL, Jin YN (2012) An effective and safe supplement for stem cells expansion ex vivo: cord blood serum. Cell Transplant 21:857-869.   DOI   ScienceOn
20 Minguell JJ, Erices A, Conget P (2001) Mesenchymal stem cells. Exp Biol Med 226:507-520.
21 Moriguchi H, Chung RT, Sato C (2010) Tumorigenicity of human induced pluripotent stem cells depends on the balance of gene expression between p21 and p53. Hepatology 51:1088-1089.   DOI   ScienceOn
22 Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147.   DOI   ScienceOn
23 Prockop DJ (1997) Marrow stromal cells as stem cells for nonhematopoietic tissues. Science 276:71-74.   DOI   ScienceOn
24 Qiu P, Bai Y, Liu C, He X, Cao H, Li M, Zhu H, Hua J (2012) A dose-dependent function of follicular fluid on the proliferation and differentiation of umbilical cord mesenchymal stem cells (MSCs) of goat. Histochem Cell Biol 138:593-603.   DOI   ScienceOn
25 Shahdadfar A, Fronsdal K, Haug T, Reinholt FP, Brinchmann JE (2005) In vitro expansion of human mesenchymal stem cells: choice of serum is a determinant of cell proliferation, differentiation, gene expression, and transcriptome stability. Stem Cells 23:1357-1366.   DOI   ScienceOn
26 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A (2000) Embryonic stem cell lines from human blastocysts : Somatic differentiation in vitro. Nat Biotechnol 18:399-404.   DOI   ScienceOn
27 Richter K, Haslbeck M, Buchner J (2010) Life on the verge of death: the heat shock response revisited. Mol Cell 40:253-266.   DOI   ScienceOn
28 Sengupta S, Peterson TR, Sabatini DM (2010) Regulation of the mTOR complex 1 pathway by nutrients, growth factors, and stress. Mol Cell 40:310-322.   DOI   ScienceOn
29 Shetty P, Bharucha K, Tanavde V (2007) Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cells. Cell Biol Int 31:293-298.   DOI   ScienceOn
30 Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, Luych PJ, Hsu SC, Smith J, Prockop DJ (2004) Internalized antigens must be removed to prepare hypoimmunogenic mesenchymal stem cells for cells and gene therapy. Mol Ther 9:747-756.   DOI   ScienceOn
31 Stute N, Holtz K, Bubenheim M, Lange C, Blake F, Zander AR (2004) Autologous serum for isolation and expansion of human mesenchymal stem cells for clinical use. Exp Hematol 32:1212-1225.   DOI   ScienceOn
32 Sullivan GJ, Hay DC, Park IH, Fletcher J, Hannoun Z, Payne CM, Dalgetty D, Black JR, Ross JA, Samuel K, Wang G, Daley GQ, Lee JH, Church GM, Forbes JS, Iredale JP, Wilmut I (2010) Generation of functional human hepatic endoderm from human induced pluripotent stem cells. Hepatology 51:329-335.   DOI   ScienceOn
33 Tekkatte C, Vidyasekar P, Kapadia NK, Verma RS (2012) Enhancement of adipogenic and osteogenic differentiation of human bone-marrow-derived mesenchymal stem cells by supplementation with umbilical cord blood serum. Cell Tissue Res 347:383-395.   DOI   ScienceOn
34 Sun YL, Ping ZG, Li CJ, Sun YF, Yi KL, Chen L, Li XY, Wang XL, Zhou X (2011) Comparative proteomic analysis of follicular fluids from normal and cystic follicles in sows. Reprod Domest Anim 46:889-895.   DOI   ScienceOn
35 Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872.   DOI   ScienceOn
36 Tateishi K, Ando W, Higuchi C, Hart DA, Hashimoto J, Nakata K, Yoshikawa H, Nakamura N (2008) Comparison of human serum with fetal bovine serum for expansion and differentiation of human synovial MSC: potential feasibility for clinical applications. Cell Transplantat 17:549-557.   DOI   ScienceOn
37 Thomson SR, Wishart TM, Patani R, Chandran S, Gillingwater TH (2012) Using induced pluripotent stem cell (iPSC) to model human neuromuscular connectivity: promise or reality? J Anat 220:122-130.   DOI   ScienceOn
38 Wei X, Wang CY, Liu QP, Li J, Zhao FT, Lian JQ, Xie YM, Wang PZ, Bai XF, Jia ZS (2008) In vitro hepatic differentiation of mesenchymal stem cells from human fetal bone marrow. J Int Med Res 36:721-727.   DOI   ScienceOn
39 Wollert KC, Meyer GP, Lotz J, Ringes-Lichtenberg S, Lippolt P, Breidenbach C, Fichtner S, Korte T, Horning B, Messinger D, Arseniev L, Hertenstein B, Graser A, Drexler H (2004) Intracornonary autologous bone-marrow cell transfer after myocardial infarction: the BOOST randomized controlled clinical trial. Lancet 364:141-148.   DOI   ScienceOn
40 Woodbury D, Schwarz EJ, Prockop DJ, Black IB (2000) Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61:364-370.   DOI   ScienceOn
41 Yamamoto N, Isobe M, Negishi A, Yoshimasu H, Shimokawa H, Ohya K, Amagasa T, Kasugai S (2003) Effects of autologous serum on osteoblastic differentiation in human bone marrow cells. J Med Dent Sci 50:63-69.
42 Yamanaka S (2009) Elite and stochastic models for induced pluripotent stem cell generation. Nature 460:49-52.   DOI   ScienceOn