• 대한수의학회지
• /
• 제41권1호
• /
• pp.105-111
• /
• 2001

We performed this study to determine the effect of Jiegeng(Platycodon grandiflorum), Danggui(Angelica sinensis), Gancao(Glycyrrhiza glabla), Chaihu(Bupleurum falcatnosa), Shoudehuang(Rehmannia glutinosa), Huangqi(Satragalus membranaceus), Muxiang(Saussurea lappa), Yuanzhi(Polygala tenuifolia), Rensen(Panax ginseng) and Baishaoyao(Paeonia lactiflolia), as Oriental radices herbs, on jejunal crypt survival, endogenous spleen colony formation and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of ${\gamma}-radiation$. Jiegeng(p<0.005), Danggui(p<0.0005), Gancao(p<0.005), Chaihu(p<0.05), Muxiang(p<0.05), Rensen(p<0.005) and Baishaoyao(p<0.005) were effective in intestinal crypt survival. Danggui(p<0.05), Chaihu(p<0.05), Shoudehuang(p<0.05), Huangqi(p<0.05), Rensan(p<0.005) and Baishaoyao(p<0.05) increased the formation of endogenous spleen colony. The frequency of radiation induced apoptosis was also reduced by pretreatment with Chaihu(p<0.05), Muxiang(p<0.005), Yuanzhi(p<0.05), Rensan(p<0.05) and Baishaoyao(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Danggui, Chaihu, Muxiang, Rensan and Baishaiyao might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• Journal of Radiation Protection and Research
• /
• 제26권1호
• /
• pp.13-18
• /
• 2001

한의학에서 근경류(rhizomata)에 분류되어 있으며 각종 보익탕제에 주로 사용되는 대표적 생약인 백출(Baizhu, Atractylodes japonica), 천궁(Chuanxiong, Cnidium officinale), 산약(Shanyao, Discorea japonica) 및 승마(Shengma, Cimicifuga heracleifolia)의 방사선 방호효과를 확인하기 위해서 고선량 및 저선량의 방사선을 마우스에 조사하고 소장움 생존, 내재성 비장집락형성 및 apoptosis 유발 등을 관찰하였다. 방사선조사전 승마 투여 군에서 소장움세포의 생존율을 증가시켰으며 내재성 비장집락 형성 시험에서는 천궁 투여 군에서 평균치의 증가가 관찰되었고 천궁 및 승마는 저선량 방사선에 의한 apoptosis 형성을 억제시켰다. 이상의 결과에서 승마 및 천궁의 방사선 방호효과를 조혈세포의 생존과 회복, 소장움세포 생존을 통하여 확인하였으며 이는 독성이 적은 천연물이라는 관점에서 방사선 방호 식품으로서 적용이 가능할 것으로 사료된다.

• 한국식품영양과학회지
• /
• 제28권2호
• /
• pp.444-451
• /
• 1999

We performed this study to determine the radioprotective effect of Sam Ryung Baek Chul San (San Ling Bai Shu San), as a prescription of traditional Oriental medicine, and its major ingredients. Jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in irradiated mice with high and low dose of rays. Sam Ryung Baek Chul San administration before irradiation increased the formation of endogenous spleen colony(p<0.05) and reduced the frequency of radiation induced apoptosis(p<0.05). In the experiment on the effect of ingredients of Sam Ryung Baek Chul San, the result indicated that the extracts of Panax ginseng, Poria cocos and Coix lacryma jabi might have the major radioprotective effects. Although the mechanisms of these inhibitory effects remain to be elucidated, these results indicated that Sam Ryung Baek Chul San might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• 한국식품영양과학회지
• /
• 제28권3호
• /
• pp.698-704
• /
• 1999

In this study, we evaluated the radioprotective effects of Kuei Pi Tang and its ingredients, as a prescription of traditional Oriental medicine. The jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells were investigated in mice irradiated with high and low dose of ${\gamma}$ rays. In the mice administered with Kuei Pi Tang extract before irradiation, the jejunal crypts were protected significantly(p<0.005) and the frequency of radiation induced apoptosis was reduced(p<0.05). In the experiment on the effects of the ingredients of Kuei Pi Tang, the results indicated that the extract of Dangui(Angelica sinensis), Suanzaoren(Zizyphus valgaris), Rensan (Panax ginseng), Fuling(Poria cocos) and Muxiang(Saussurea lappa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indi cated that Kuei Pi Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• 제1권1호
• /
• pp.45-55
• /
• 1998

목 적: 우유 과민성 장병증(cow's milk-sensitive enteropathy, CMSE)은 우유를 식이 하였을 때 유해 반응과 함께 발생하는 상부 소장 점막의 손상이 병리적 특징으로 관찰되며, 이러한 점막의 손상은 우유를 제거하면 회복될 수 있고, 다시 유발시험을 하면 재발될 수 있다. CMSE 환자에서 소장 점막 손상의 정도를 형태학적 계측을 이용하여 정량적으로 분석하여 보고, 이를 이용한 CMSE의 진단적 가치 및 병리적 특성에 관하여 알아보고자 본 연구를 시행하였다. 대상 및 방법: 1994년 3월부터 1996년 6월까지 만성 설사와 성장 장애를 주소로 대구효성가톨릭 대학병원 소아과에 입원한 환자에서 우유에 대한 유발, 제거시험에 따른 임상적 반응 및 상부위장 관내시경을 시술하여 얻게 된 십이지장부의 생검조직 소견을 통하여 CMSE로 진단된 생후 $30.4{\pm}11.7$(14~56)일된 13명의 환아를 대상으로 하였다. 소장 생검조직의 형태학적 계측을 이용하여 융모 높이(villous height, VH), 음와 영역 깊이(crypt zone depth, CZD), 점막전체 두께(total mucosal thickness, TMT), 음와 영역에 대한 융모의 상대적 높이(VH/CD), 융모 1 mm당 상피세포간 림프구의 수(interepithelial lymphocytes, IEL), 0.5 mm의 점막근육판(muscularis mucosa)에 해당하는 상피 표면의 길이(length of surface epithelium, LSE), 상피층의 호산구 침윤(EOS-EP), 고유층(lamina propria)의 호산구 침윤(EOS-LP)을 측정하고, 대조군 및 회복기 생검 조직과 비교하였다. 결 과: 1) VH는 CMSE 환자군에서 $135{\pm}59\;{\mu}m$으로 대조군 $408{\pm}48\;{\mu}m$에 비하여 유의하게 낮았으며(p<0.001), 전례에서 융모 위축의 소견이 관찰되었다. 2) CZD는 CMSE 환자군에서 $311{\pm}65\;{\mu}m$으로 대조군 $188{\pm}24\;{\mu}m$에 비하여 유의하게 깊었다(p<0.05). 3) TMT는 CMSE 환자군에서 $449{\pm}56\;{\mu}m$으로 대조군 $596{\pm}71\;{\mu}m$에 비하여 유의하게 낮았다(p<0.001). 4) VH/CD는 CMSE 환자군에서 $0.46{\pm}0.28$로 대조군 $2.17{\pm}0.12$에 비하여 유의하게 낮았다(p<0.001). 5) LSE는 CMSE 환자군에서 $889{\pm}231\;{\mu}m$으로 대조군 $2547{\pm}378\;{\mu}m$에 비하여 유의하게 낮았다(p<0.001). 6) IEL은 CMSE 환자군에서 $34.1{\pm}10.5$개로 대조군 $13.6{\pm}3.6$개에 비하여 유의하게 높았다(p=0.001). 7) EOS-EP는 CMSE 환자군에서 $0.47{\pm}0.83$개로 의미 있는 침윤은 관찰되지 않았으며, 대조군 $0.80{\pm}0.84$개와 유의한 차이는 보이지 않았다. EOS-LP는 환자군에서 $2.67{\pm}3.72$개로 의미 있는 침윤은 관찰되지 않았으며, 대조군 $4.0{\pm}2.5$개와 유의한 차이를 보이지 않았다. 8) 회복기의 형태학적 변화는 VH $320{\pm}34\;{\mu}m$, CZD는 $194{\pm}19\;{\mu}m$, TMT $514{\pm}21\;{\mu}m$, VH/CD $1.41{\pm}0.25$, LSE $1840{\pm}210\;{\mu}m$, IEL $10.8{\pm}2.3$개로 관찰되었다. 결 론: 소장 조직의 생검은 상부위장관내시경을 이용하여도 그 임상적 유용성이 높으며, CMSE 환자에서 소장 점막의 손상은 형태학적 계측을 통하여 정량적으로 평가할 수 있으며 진단적 민감도가 높다. CMSE에서 점막의 손상은 빠른 융모 상피의 손상에 대한 음와 세포의 충분한 보상의 실패로 사료되며, 단백질 가수분해물의 수유후 빠른 회복이 관찰되었다.

• Journal of Radiation Protection and Research
• /
• 제12권2호
• /
• pp.1-8
• /
• 1987

원자력병원(原子力病院) 싸이클로트론 중성자선(中性子線)의 방사선생물학적(放射線生物學的) 특성파악을 위하여 생쥐를 이용하여 RBE를 측정하였다. 단일전신조사(單一全身照射)에 의한 생쥐공장소양선세포(空腸小襄腺細胞)의 RBE는 2.8이었다. 이 RBE치(値)는 상이(相異)한 조직(組織)이나 기관(器管)종류에 있어서 분할(分割), 조사방법(照射方法)의 변화에 따라 상이(相異)하게 변하였다. 위장관(胃腸管) 및 골수(骨髓)의 단일조사(單一照射)에 대한 RBE는 각각 1.5 및 1.9였고, 분할조사시(分割照射時) 1회, 조사량(照射量), 총조사량(總照射量) 및 분할(分割)횟수 등에 따른 RBE의 변화를 관찰하였다.

• International Journal of Internet, Broadcasting and Communication
• /
• 제12권2호
• /
• pp.98-112
• /
• 2020

There is a strong connection between the diet rich in antioxidants and the decreased incidence of inflammatory bowel disease and cancerous diseases. Diets that are rich in anti-oxidants particularly include fruits and vegetables containing the high amounts of vitamin A-E, carotenoids, and minerals. The supercritical heat-treated radish extracts of the research result had an inhibitory effect on the development of aberrant crypt foci (ACF), namely, preneoplastic lesions having a potential to become cancer cells and reduced the number of the aberrant crypt foci (ACF) consisting of four or more aberrant crypts (AC) having high risk to become tumors by about half. The supercritical heat-treated radish extracts can reduce the incidence of preneoplastic lesions having a high risk of developing cancer by about 28 %. DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. Supercritical heat-treated radish extracts, as well as sulfasalazine, suppressed colonic length and mucosal inflammatory infiltration. In addition, supercritical heat-treated radish extracts treatment significantly reduced the expression of pro-inflammatory signaling molecules through suppression both mitogen-activated protein kinases (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways, and prevented the apoptosis of colon. Moreover, supercritical heat-treated radish extracts administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권9호
• /
• pp.5031-5035
• /
• 2013

Zizyphus spina-christi (ZSC) fruit is a rich source of bioactive compounds but any medicinal properties in chemoprevention of colon cancer have hitherto not been studied. The aim of the present study was to examine in vivo protective effects of ZSC water extract on colon carcinogenesis in azoxymethane (AOM)-treated rats. Our results showed that ZSC significantly reduced AOM-induced colonic aberrant crypt foci development and AOM-induced oxidative stress as indicated by restoration of endogenous glutathione depletion and abrogating the impairment of total antioxidant capacity. Caspase-3 cleavage, which has been considered as an apoptotic index, was almost undetectable in AOM-treated rats and ZSC exhibited pro-apoptotic effects evidenced by increased levels of cleaved caspase-3. In the studied model, our findings provide the first in vivo evidence that ZSC extract could inhibit the early stage of colon carcinogenesis by preventing oxidative stress and inducing apoptosis.

• 대한수의학회지
• /
• 제37권1호
• /
• pp.167-176
• /
• 1997

The present investigation was focussed mainly on the development of the tumors and proliferating cells on the intestinal tracts of 1, 2-dimethyl-hydrazine(DMH)-treated young or adult rats. 26 rats(Wistar, 14 young rats weighting approximately 130~180gm and 12 adult rats weighting approximately 500~550gm) were given subcutaneously once weekly with 20mg of DMH/kg body weight(BW)/week for 8~22 weeks. Individual body weight were recorded weekly at the same day and time. The rats were killed at 8, 13, 15. 17, 19, 21 and 22 weeks. The intestinal tracts were opened longitudinally and carefully examined for tumors. The localization, number, and size of tumors were noted. Tumor-bearing areas were dissected out and fixed on neutral buffered 10% formalin and normal-looking mucosa from 8~22 weeks rats were also taken for fixation. Paraffin sections were stained by H-E for histopathological examination or with immunohistochemical stain for bromodeoxyuridine(Brdur) positive cells. 1. The growth proportion of body weight appeared to be decreased in the DMH-treated young rats than in control young rats and body weight of DMH-treated adult rats appeared to be 13.4% or less lower than weighted on 0 week. 2. Macroscopically, the developed tumors in the intestinal tracts were not observed as early as the 13 weeks after DMH treatment. The number of developed tumors per rat was found to be 14.3, 18.8, 22.3 in 15, 17 and 22 weeks. The numbers of tumors in intestinal regions per rat were 2.1, 4.3, 5.4, 2.5 in duodenum, jejunum, ilium and colon on 15 weeks, 2.3, 6.4, 7.8, 2.3, on 17 weeks, and 2.7, 9.3, 9.0, 1.3 on 22 weeks, respectively and the ileum and jejunum were higher in appearance rate of tumors and tumor types are dome shapes and diameter of largest tumor were 6.3mm. 3. Histopathologically, intestinal mucosa were thickened by the irregular distorted and distended crypts following hyperplasia. The tumors developed on the mucosa and submucosa and were recognized to be adenocarcinoma. 4. Immunohistochemically, the labeling index(LI) was calculated as the ratio of the number of Brdur-labeled cells to the total number of column cells of the crypts with longitudinal axis. LI of Brdur positive cells per crypt were 5.6%, 8.0% on small intestine of control and 22 week group, respectively and 3.7%, 12.7% on large intestine of control and 22 week group, respectively and were appeared to be increase in 22 week group than in control group and to be more number of proliferating cells in 22 week group than in control group. 5. LI of Brdur positive cells in 1, 2, 3, 4, 5 segments of crypt column were 11.7%, 10.7%, 3.8%, 0.6%, 0% in small intestine of control group and 23.5%, 11.8%, 2.3%, 2.4%, 0.8% in small intestine of 22 week group, and 5.4%, 7.4%, 3.8%, 1.0%, 0.4% in large intestine of control group and 29.5%, 20.3%, 5.9%, 6.3%, 1.3% in large intestine of 22 week group respectively. So results indicate that the number of proliferating cells by DMH treatment increase and were concentrated on the 1, 2 segments of crypt columns.

• 대한수의학회지
• /
• 제52권2호
• /
• pp.115-124
• /
• 2012

Colorectal cancer (CRC) is one of the leading causes of cancer death in western countries or in the developed countries. Zinc intake has been associated with decreased risk of CRC. We investigated the effect of zinc on the formation of colonic aberrant crypt foci (ACF) induced by azoxymethane followed by dextran sodium sulfate in mice. Five-week old ICR mice were fed with the different zinc levels (0.01, 0.1, 1 ppm) for 12 weeks. The numbers of ACF were measured in the colonic mucosa. The ACF number of HZn group was significantly low compared with LZn group or MZn group. Cytosolic superoxide dismutase activity was the highest in HZn group, while thiobarbituric acid reactive substance level for lipid peroxidation was the highest in LZn group. There was no difference in number of PCNA-positive proliferative cells among the groups. TUNEL-positive apoptotic cells were increased in HZn group compared with LZn group. The HZn group exhibited a decrease of ${\beta}$-catenin immunostaining areas compared with the LZn or MZn group. These findings indicate that dietary zinc might exert a protecting effect against colon carcinogenesis by inhibiting the development of ACF in the mice.