• Korean Journal of Microbiology
• /
• v.48 no.3
• /
• pp.225-227
• /
• 2012

Lignin degrading enzymes from white rot fungi show broad substrate specificities, and therefore they can degrade variety of recalcitrant compounds. We have used three different protocols for the generation of immobilized laccase and manganese peroxidase crude enzymes from the genetically transformed strains of Merulius tremellosus Fr. These immobilized enzymes were used in the decolorization of Remazol Brilliant Blue R (RBBR), and they showed about 75% decolorization rates during the 48 h reactions. Although the decolorization efficiency decreased by 10-15% after a repeated use of the immobilized enzymes, these can be reused in various degrading reactions.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.30 no.4
• /
• pp.388-397
• /
• 1985

Forage productivity and sequential changes in forage quality of four rye and two triticale varieties were studied. All the observed characteristics were similar among rye or triticale varieties, but they were different between rye and triticale varieties. Early growth of rye was better compared to triticale varieties, but at heading stage yield in dry weight, digestible dry matter and percent dry matter of triticale were higher compared to rye varieties. Heading stage of rye was earlier than that of triticale by 11 - 14 days. Triticale had longer and broader leaf blades and a higher leaf blade/total dry weight ratio compared to rye. Protein content decreased as plant growth advanced and it was higher in rye before heading, but it was similar after heading compared to triticale. Cellulose and lignin contents of rye increased through 20 days after heading. However, in triticale cellulose increased until heading stage and it levelled off, but lignin content increased rapidly after heading. Among the crude fibers, only acid detergent fibers(ADF) was negatively correlated with in vitro dry matter digestibility(IVDMD) in both rye and triticale. IVDMD of rye decreased rapidly after heading, but it was maintained as high as heading stage up to 15 days after heading in triticale. Protein content and IVDMD in leaf blades were higher than those of culm + leaf sheath, hemicellulose was similar, and cellulose, lignin, and ADF in leaf blades were lower compared to culm + leaf sheath. In the early and middle part of April rye was superior to triticale as a soiling crop because of a better plant growth of rye under the low temperature conditions, but in the later part of April and early part of May triticale was superior to rye because of a rapid decreased in IVDMD of rye after heading and a late maturing characteristics of triticale. Planting both rye and triticale could prolong the utilization period of the soiling crops and increase in yield with better quality compared to a single crop of rye or triticale.

• Asian-Australasian Journal of Animal Sciences
• /
• v.3 no.4
• /
• pp.353-361
• /
• 1990

A number of correlation and regression analyses were performed on data from apparent and true digestibility of amino acids at ileal and fecal level with finishing pigs, in order to investigate whether these amino acid digestibilities could be predicted with certainly degree on their fiber fractions content (chemical analysis). The data comprised 16 diets varying in 4 levels of crude fiber and from 4 fiber sources. The relationships between fiber fractions including crude fiber (CF), NDF, ADF lignin and cellulose contents on apparent and true digestibility of almost all amino acids in both ileal and fecal level were negative, except glutamine at fecal level. In apparent digestibility at ileal level, the correlations of fiber fractions were moderate (r of NDF = 0.53 to 0.63; ADF, 0.50 to 0.77; cellulose, 0.50 to 0.75), with an exception of CF content was relatively high (r of 0.58 to 0.81). The correlations to true digestibility of amino acids were weaker. In case of at fecal level, the higher correlation (negative) was found with NDF than CF content. Estimations of amino acids digestibility were performed using regression equation. The data showed that apparent digestibility of amino acids could be estimated for almost amino acids except arginine, threonine, valine and tyrosine at fecal level and phenylalaine, valine and glycine at ileal level. The best prediction at ileal and fecal level ($r^2=0.55-0.77$ and 0.52-0.76), respectively was obtained with NDF content. Prediction for true digestibility of amino acids, none of fiber fractions could be estimated for arginine, leucine and valine at all collection levels. At ileal level, CF could be used for most of amino acids except phenylalanine, glycine and praline; cellulose, only for lysine and methionine and NDF, only for proline. At fecal level, glutamine digestibility could be estimated only from CF and ADF content. The best predictor at ileal level was ADF content whereas at fecal level was NDF content. These results indicate that lignin content could not be used as predictor for all amino acids at both levels neither in apparent nor true digestibility.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.26 no.4
• /
• pp.221-226
• /
• 2006

This study was conducted to investigate the effect of tall type mixtures and turf type mixtures on dry matter yield, botanical composition and quality. The experimental design includes two mixture types : Tall type mixtures; orchardgrass (Potomac) 50+tall fescue (Fawn) 20+ Kentucky bluegrass (Kenblue) 10+red clover (Kenland) 20%) and turf type mixtures; Kentucky bluegrass(Newport) 60%+tall fescue (reboil Jr.) 20+perennial ryegrass (palmer II) 20%. The DM yield was higher obtained in tall type mixture than that of turf type mixture (p<0.05). In the chemical composition, Turf type mixture was higher in crude protein (CP) and dry matter digestibility (DMD), but lower in fibrous compound as NDF, ADF, cellulose and lignin than those of tall type mixture (p<0.05). The crude protein dry matter (CPDM) and digestible dry matter (DDM) yield was higher in tall type mixture than turf type mixture (p<0.05). In this experiment, nutritive value of turf type mixture was higher than that of tall type mixture, but CPDM and DDM yield of turf type mixture were lower than that of turf type mixture due to low dry matter yield. On the other hand, turf type mixture was obtained high feed value, and maintaining the various botanical composition on the 13 th cutting frequency condition. therefore, turf type mixture had a utilization possibility under grazing livestock as sheep and milk goats.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.9 no.3
• /
• pp.179-186
• /
• 1989

According to the results from the experiments of selecting a suitable variety of forage rape and comparing productivity and nutrient quality between forage rape and oil seed rape, Velox appeared to be the most suitable variety in terms of productivity and nutrient quality at the southern area of Korea among the varieties used in the experiments. Consequently, Velox was grown under two different cultural methods, individual culture and population culture, and productivity and variation of nutrient quality during the growth period were observed and compared between the cultural methods. The results from the experiment are summarized as follows: 1. Although variations were great in plant fresh weight and plant dry matter weight among plants, individual culture showed significantly higher value in these characters at the 1 % level than population culture. Plant fresh weight and plant dry matter weight were largely dependent upon the amount of branches in case of individual culture. However, in population culture, they were equally dependent upon the amount of branches and main stem. There was no significant difference in dry matter percentage between cultural methods, and main stem showed highest dry matter percentage. 2. Content of crude protein was decreasing gradually as plants continued to grow. Individual culture showed higher content of crude protein than population culture from 90 to 120 days after sowing but vice versa from 180 days after sowing to flowering stage. Contents of fiber such as NDF, ADF, cellulose and lignin was low at the early stage of growth. It was increasing gradually as plants grew older and at the latter stage of growth plants under individual culture showed higher values in contents of fiber. 3. In vitro dry matter digestibility (IVDMD) of both stems and leaves was decreasing gradually as plants grew older. Plants under individual culture showed higher IVDMD of stems than plants under population culture, but no significant difference in IVDMD of leaves was observed between cultural methods.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.4
• /
• pp.335-340
• /
• 1989

In order to find out the optimum harvest (clipping) date combined with sowing date on yield and nutrient Quality of forage rape which is suitable at the southern part of Korea, Velox, introduced variety was grown from Sep. 1987 to May 1988 at Sunchon National University, and yield and nutrient Quality of plant were observed. As harvest date and sowing date were delayed, the plant length was longest, number of branch and leaves per main stem were increased in the time of full bloom clipping and Sep. 24 sowing but stem diameter was thickest in the early sowing. Fresh yield was heaviest in the time of flowering clipping and Sep. 24 sowing but the most dry matter yield was heaviest in the time of full bloom clipping and Sep. 24 sowing. Content of crude protein was highest and that of crude fiber such as NDF, ADF, hemicellulose, cellulose and lignin were lowest in the late time of clipping and sowing. Further more IVDMD was high and dry matter yield and digestible dry matter yield were highest in the time of flowering clipping and Sep. 24 sowing. Judging from the results reported above, at optimum harvest (clipping) date combined with sowing date for yield and nutrient Quality of forage rape seemed to be the time of flowering clipping and Sep. 24 sowing.

• Journal of the Korean Wood Science and Technology
• /
• v.28 no.3
• /
• pp.25-33
• /
• 2000

This study was carried out to find the optimal isolation conditions of xylan from steam-exploded materials, such as rice straw(Oryza sativa), barley straw(Hordeum vulgare) and oak wood(Quercus mongolica), In the chemical composition, we found that the contents of water-extractives and ash of rice straw and barley straw were more than those of oak wood. Rice straw, barley straw and oak wood were steam-exploded at 20kgf/$cm^2$ for 3 minutes or 6 minutes. The content of lignin in three different steam-exploded materials was higher than that of non-treated materials. The crude xylan was extracted with hot water and 0.5% KOH solution from steam-exploded materials. In the sugar type of crude xylan extracted with hot water and 0.5% KOH solution, the oligomer content of crude xylan extracted with hot water was much more than that of crude xylan extracted with 0.5% KOH solution. The crude xylan was purified with 5% barium hydroxide Solution and ethanol precipitation procedure. The content of xylose of purified xylan was over 85%, but other sugar residues(arabinose, mannose, galactose and glucose) were not removed completely.

• Asian-Australasian Journal of Animal Sciences
• /
• v.8 no.2
• /
• pp.163-169
• /
• 1995

This study was conducted 1) to compare the nutrient content and mineral composition of the Philoppine indigenous sheep and goats diets when grazed in common pasture during rainy season, 2) to determine the influence of sampling methods on nutrient content and mineral composition of upland (UP) and lowland (LP) native pastures, and 3) to compare these two pasture types in terms of nutrient and mineral composition. Experiment 1 used sis esophageally fistulated animals, three each for sheep and goats. Weekly extrusa collection was done for a period of three months. Experiment 2 was conducted in UP and LP; these were sampled thrice by three methods, plucking, clipping and use of three esophageally fistulated sheep. Results of Experiment 1 indicated that the diets of sheep were greater in crude protein(CP; p < 0.01) and ether extract(EE; p < 0.05) but lower in crude fiber (CF) and acid detergent lignin (ADL; p < 0.05) than that of goat diets. All other nutrient fractions including mineral composition were not significantly different from each other. Significant findings of Experiment 2 were: CP content of UP was in the order fistulated > plucked > clipped (p < 0.05); CP content of LP was greater (p <0 .05) in fistulated and plucked samples; and ash content was greater (p < 0.05) in fistulated samples of both pastures. The CP of UP was different (p < 0.05) from each sampling method but the CP of LP and ADL of both pastures were greater (p < 0.05) in clipped samples. Most minerals either macro or microminerals were greater in fistulated samples of both pastures. Pooled data of the two pastures showed that LP had better nutrient characteristics and greater mineral composition. Based from this study, there were few differences between the diets of indigenous sheep and goats when grazed in common pasture. Also, sampling methods influenced the nutrient composition of indigenous sheep diets.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.8
• /
• pp.1107-1111
• /
• 2004

An experiment was conducted to evaluate the potential nutritive value of five legume forages (leaves and twigs) in West Sumatra during wet and dry seasons. The chemical composition, in vitro dry matter (IVDMD), organic matter (IVOMD) and crude protein digestibility (IVCPD), in vitro gas characteristics and estimated metabolizable energy (ME) showed variation among legume forages and between different seasons. Crude protein (CP) ranged from 14.2 to 27.8% DM in the wet season, with a significant (p<0.05) reduction in dry season. C. pubescens, G. maculata, L. leucocephala and P. phaseloides showed the least reduction in CP content. The NDF, ADF and lignin were about 39.0, 26.5 and 6.1% DM, respectively in the wet season, and significantly (p<0.05) increased in the dry season, except for NDF of C. mucunoides and C. pubescens. For IVDMD, IVOMD and IVCPD significantly increased in the wet season, but values remained as high as over 50.0% of DM. The in vitro gas characteristics and metabolizable energy were significantly (p<0.05) higher in wet season than the dry season. During both seasons, the rate of constant (c) for G. maculata and ME content for C. pubescens and P. phaseloides were not significantly (p<0.05). Results demonstrated that P. phaseloides and L. leucocephala have a good nutritive value during both wet and dry seasons. Further studies on feeding trials are needed to quantify the animal responses when offered these legume forages.

• Korean Journal of Medicinal Crop Science
• /
• v.2 no.2
• /
• pp.174-179
• /
• 1994

This experiment was carried out to get the basic information on utilizing artemisia selengensis, the chemical component of artemisia selengensis and those Artemisia sp. were analyzed and compared. The results are as followed; The contents of water, crude ash and crude fiber in Artemisia selengensis were higer than in Artemisia sp. the contents of thiamin, riboflavin and ascorbic acid in Artemisia selengensis were a little lower than those in Artemisia sp. while niacin in Artemisia selengensis was a little higher than those in Artemisia sp. The content of dietary fiber were 40.91% for Artemisia selengensis and 4166.7mg% for Artemisia sp. and the contents of Na, Fe and Mn in Artemisia selengensis were over twice as a high as those in Artemisia sp. While the content of Cu was Artemisia selengensis was lower than that in Artemisia sp. The content of phenolic compound in Artemisia selengensis was a little higher than those in Artemisia sp.