• Title/Summary/Keyword: crude extract

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A Study on the General Components and Minerals in Parts of Omija (Schizandra Chinensis Baillon) (오미자의 부위별 일반성분과 무기질함량에 관한 연구)

  • Lee, Joung-Sook;Lee, Mi-Gyeung;Lee, Sung-Woo
    • Journal of the Korean Society of Food Culture
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    • v.4 no.2
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    • pp.173-176
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    • 1989
  • This study was carried out to comparison of the contents of general and mineral components between the original sample and water extract in each part of omija. The content of Carbohydrate was highest in endocarps, and that the crude protein and crude lipid in seeds. The contents of K and Mn in the fruits, endocarps, and seeds were all higher than those of the other cations. The content of K and Zn in endocarps were three to four times as much as those of seeds. and the content of Na, Ca, and Cu in endocarps were 1.5 to 1.75 times of seeds. Mineral contents by water extract in each part were ordered as K, Mg, Ca, and Fe. As the percentage of each ion in water extract on the basis of original sample, Fe was the highest ratio of behavior, and Mn was lowest.

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The Bioefficacy of Methanol Crude Leaf Extract of Acacia melanoxylon (Acacia melanoxylon 잎 메탄올 추출액의 생물활성)

  • M. Jayakumar;M.;Manikadan;Eyini, M.
    • The Korean Journal of Ecology
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    • v.21 no.6
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    • pp.805-808
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    • 1998
  • The methanolic crude leaf extract of Acacia melanoxylon shows inhibitory effects on seed germination, shoot growth, root growth and biomass of black gram (Phaseolus mungo L. Co 1) and weeds (Cassia occidentalis L. and Cyperus rotundus L.). The post emergence treatment was given on the 8th day after sowing and 4-6 days after seedling emergence. After postemergence treatment with 20% extract concentration, wilting symptoms appeared in P. mungo and C. occidentalis while necrosis occurred in C. rotundus. The seedling growth was inhibitory at all concentrations (5%, 10%, 20%) as compared to control plants. This inhibitory effect may be due to the presence of allelochemicals like phenolics in the A. melanoxylon leaves. Different types of phenolic acids were identified.

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Inhibitory Effect of the Extract from the Three Crude Drugs, Kalopanax pictus, Pueraria thunbergiana and Rhus verniciflua on NO and TNF-a production in LPS-induced Macrophage 264.7 Cells

  • Kim, In-Tae;Lee, Kyung-Tae;Choi, Jong-Won;Park, Hee-Juhn
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.155.2-155.2
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    • 2003
  • We previously reported the antimutagenicity, anti-lipid peroxidation and antiinflammtory properties of the three Chinese herb medicine, Kalopanax pictus, Pueraria thunbergiana and Rhus verniciflua, which have been used as therapeutics for diabetes mellitus and inflammation in Korea. Since these drugs have been used for the prescriptions consisted of several crude drugs, we investigated whether the extract of the three drugs could increase the individual drugs or not. The three extracts were independently prepared from K. pictus, P. thunbergiana, and R. verniciflua, which extracts were named the K-1, P-1 and R-1 in this order and the extract (KPR-1) was also prepared from the combined plant materials with each same weight of the three drugs. (omitted)

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Enhancement of Polyphenol Content and Antioxidant Activity of Brown Alga Eisenia bicyclis Extract by Microbial Fermentation

  • Eom, Sung-Hwan;Kang, Young-Mi;Park, Jae-Hong;Yu, Dae-Ung;Jeong, Eun-Tak;Lee, Myung-Suk;Kim, Young-Mog
    • Fisheries and Aquatic Sciences
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    • v.14 no.3
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    • pp.192-197
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    • 2011
  • The objective of this study was to select an effective microbial strain to improve the functional qualities of Eisenia bicyclis water extract by fermentation. For this purpose, several microorganisms isolated from traditional Korean fermented foods were inoculated and cultivated in E. bicyclis water extract. Ultimately, yeast strain YM-1 was selected for further study based on its total phenolic compound (TP) content and antioxidant activity, which were enhanced by microbial fermentation. The extract fermented by YM-1 exhibited a superior TP content and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to extracts fermented by other microbes. The highest TP content and DPPH radical scavenging activity were observed after one day of YM-1 fermentation. Yeast strain YM-1 was identified as Candida utilis based on an analysis of its physiological characteristics. During fermentation of the extract by C. utilis YM-1, no significant difference was observed in the proximate composition, including moisture, crude lipid, crude protein, and crude ash. Fermentation by C. utilis YM-1 resulted in enhanced biological activity, including increases in the TP content and antioxidant activity. Thus, fermentation by C. utilis YM-1 is an attractive strategy for developing value-added food ingredients.

Neuronal Cell Protective Effects of Methanol Extract from Cheonggukjang Using in vitro System (In vitro system에서 청국장 메탄올 추출물의 신경세포 보호효과)

  • Jeong, Chang-Ho;Kwak, Ji-Hyun;Kim, Ji-Hye;Choi, Gwi-Nam;Jeong, Hee-Rok;Heo, Ho-Jin
    • Korean Journal of Food Science and Technology
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    • v.42 no.6
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    • pp.768-772
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    • 2010
  • In this study, the neuronal cell protective effects of methanol extract from cheonggukjang were evaluated. The proximate composition and total phenolics of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fiber, and 28.43 mg/g of total phenolics. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when methanol extract was present in the media compared to PC12 cells treated with $H_2O_2$ only. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into the medium was also inhibited. Furthermore, the inhibitory effect of the methanol extract against acetylcholinesterase was dose-dependent.

Changes in Composition of Gugija (Lycii Fructus) Species According to Harvest Time (수확시기에 따른 구기자 품종의 성분변화)

  • Lee, Hee-Chul;Lee, Bong-Chun;Kim, Su-Dong;Lee, Ka-Soon;Paik, Seung-Woo;Lee, Sox-Su;Kim, Seong-Min
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.5
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    • pp.306-312
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    • 2008
  • Total sugar, crude protein, crude lipid, ash, total polyphenol, extract and betaine contents of various Lycii fructus species (Cheongyang jaerae, Myungan, Bulro, Cheongdae, Jangmyung, Cheongwoon, Cheongyang NO.6, and Cheongyang NO.7) were investigated according to harvest time at intervals of one momth from mid August to mid November. In Cheongyang NO.6, average content of total sugar and extract were the highest, 28.91% and 62.31%, respectively. In Cheongyang NO.7, crude protein (3.24%), total polyphenol (2.13%), betaine(10.77 mg/g) were the highest and crude lipid (6.82%) was the higest in Cheongdae species, and ash (5.83%) was the highest in Cheongwoon species. At the most of the species (except Cheongyang jaerae, Myungan) crude protein, crude lipid, ash and betaine contents were increased, and total sugar, total polyphenol and extract contents were decreased according to harvest time was lated. Also, a relationship among the components of Lycii fructus was found out. ; excract contents (r = 0.81) were increased as total sugar contents were increased, while crude protein (r = -0.88), crude lipid (r = -0.82), ash contents (r = -0.81) were decreased as that And betaine contents (r = 0.90) were increased as total polyphenol contents were increased.

Antioxidant and analgesic activity of Clerodendrum visconsum leaf

  • Rahman, Shafiur;Sarder, Mokaddez;Shilpi, Jamil Ahmad;Hasan, Choudhury Mahmud
    • Advances in Traditional Medicine
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    • v.6 no.4
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    • pp.319-323
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    • 2006
  • The crude ethanolic extract of the leaves of Clerodendrum viscosum (Family: Verbenaceae) was evaluated for its antioxidant and analgesic activities to investigate the scientific basis of the traditional uses. The antioxidant property of the extract was assessed by 1, 1-diphenyl -2- picryl hydrazyl (DPPH) free radical scavenging assay. The extract showed prominent antioxidant activity ($IC_{50}about{\sim}16{\mu}g/ml$) which was comparable to standard drug ascorbic acid ($IC_{50}about{\sim}15{\mu}g/ml$).The extract produced significant (P<0.001) writhing inhibition in acetic acid induced writhing in mice at the dose of 125 mg, 250 mg and 500 mg/kg body weight respectively, which were comparable to the standard drug diclofenac sodium. The results tend to suggest that the crude leaves extract at the above doses have antioxidant and analgesic activities and indicate that it might possess biologically active constituents having free radical scavenging and analgesic activities respectably.

Analgesic, cytotoxic and antioxidant activities of Trewia polycarpa bark

  • Rahman, Md Shafiur;Sadhu, Shamir Kumar;Hasan, Choudhury Mahmud
    • Advances in Traditional Medicine
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    • v.6 no.2
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    • pp.121-125
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    • 2006
  • The crude ethanol extract of the stem bark of Trewia polycarpa (Family: Euphorbiaceae) was subjected to acetic acid induced writhing inhibition, Brine Shrimp lethality bioassay and 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging assay for screening of analgesic, cytotoxic and antioxidant activity respectively. The extract produced significant (P < 0.001) writhing inhibition in acetic acid induced writhing in mice at the dose of 125, 250 and 500 mg/kg body weight respectively, which were comparable to the standard drug diclofenac sodium. The extract showed significant lethality to Brine Shrimp and the $LC_{50}$ value was $8\;{\mu}g/ml$. The extract showed prominent free radical scavenging activity ($IC_{50}$ about ${\sim}10\;{\mu}g/ml$) compare to standard drug ascorbic acid ($IC_{50}about\;{\sim}15\;{\mu}g/ml$). The results tend to suggest that the crude ethanol extract of the bark might possess analgesic, cytotoxic and antioxidant activities or active constituent(s) responsible for the activities.

Variation of Nutritional and Antioxidant Characteristics of Extract of Lycium barbarum produced by using Different Extraction Processes

  • Ho-Jong You
    • Journal of the Korean Applied Science and Technology
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    • v.41 no.2
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    • pp.520-529
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    • 2024
  • Lycium barbarum extract has a high potential to be developed as a health functional food due to the various health-promoting effects of Lycium barbarum. This study analyzed changes in nutritional and functional components depending on the extraction solvent (purified water and a mixture of purified water and alcohol) and the condition of the sample. The nutritional components (carbohydrates, protein, fat, ash), organic acids, amino acids, total phenolic compounds, and total flavonoids of the extract produced during the extraction process were analyzed. The nutritional composition and functional substances of the extracts showed some differences depending on the type of solvent and the condition of the sample. The amounts of crude protein (7.61%), crude fat (1.63%), carbohydrate (90.22%), and ash (0.54%) of dried Lycium barbarum extract using purified water as a solvent were similar to those of the powder sample extract. The highest content of citric acid was 4.31 mg/mL, similar to the case of acetic acid, when the powder sample used a mixture of purified water and alcohol as a solvent. The highest amino acid content was 357.39 mg/mL when the powder sample was mixed with purified water and alcohol as a solvent. The total amount of phenolic compounds was 686.16 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent. The highest total flavonoid content was 111.32 g/L when the powder sample was extracted with a mixture of purified water and alcohol as a solvent.

Antimicrobial Effect of Red Ginseng Against Major Food Poisoning Microorganisms Including Staphylococcus aureus (황색포도상구균을 포함한 식중독 미생물들에 대한 홍삼의 항균효과)

  • Kwak, Yi-Seong;Jung, Myung-Gyun;Jang, Kyoung-Hwa;Han, Min-Woo;Yu, Byeung-Il
    • Journal of Food Hygiene and Safety
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    • v.35 no.4
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    • pp.382-390
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    • 2020
  • This study was carried out to evaluate the antimicrobial effect of red ginseng (Panax ginseng C.A. Meyer) against several foodborne pathogens including Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger. The antimicrobial effect was determined by agar diffusion method using red ginseng extract, crude saponin and non-water-soluble fractions. Red ginseng extract showed antimicrobial effect against S. aureus, but not C. albicans or A. niger. The extract showed anti-bacterial activity at concentration above 30% against S. aureus, which cause both food poisoning and atophic dermatitis. Crude saponin showed antibacterial activity above 7.5% against the bacterium. However, the ginsenosides purified from crude saponin showed no antimicrobial activities at 100-200 ㎍/mL. To investigate the mode of growth inhibition, red ginseng extract and crude saponin were added to 0.85% NaCl solution containing S. aureus and then incubated at 35℃ for 12 h. The results showed that viable cells were rapidly reduced in above 10% concentration of red ginseng extract and above 2% of crude saponin, respectively. However, the crude saponin and red ginseng extract did not inhibit the bacterial cells completely at those same concentrations. On the other hand, whereas all non-water-soluble fractions showed inhibition zones above 10 mm against S. aureus, they showed no inhibition effects against E. coli, C. albicans or A. niger. The methanol fraction-1 (MF-1) showed the highest antibacterial activity against S. aureus, and the MIC (minimal inhibitory concentration) was 0.625 mg/mL. These results suggest that red ginseng extract, crude saponin and non-water-soluble fractions show selective antibacterial activity against S. aureus, and non-water-soluble fractions might be used as natural antibacterial agents.