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Neuronal Cell Protective Effects of Methanol Extract from Cheonggukjang Using in vitro System  

Jeong, Chang-Ho (Department of Food Science and Biotechnology, Institute of Life Science and Resources, Kyung Hee University)
Kwak, Ji-Hyun (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Kim, Ji-Hye (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Gwi-Nam (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Jeong, Hee-Rok (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Heo, Ho-Jin (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Korean Journal of Food Science and Technology / v.42, no.6, 2010 , pp. 768-772 More about this Journal
Abstract
In this study, the neuronal cell protective effects of methanol extract from cheonggukjang were evaluated. The proximate composition and total phenolics of the methanol extract were 40.95% crude protein, 22.49% crude fat, 15.99% nitrogen free extract, 7.91% moisture, 6.74% crude ash, 5.92% crude fiber, and 28.43 mg/g of total phenolics. Intracellular ROS accumulation resulting from $H_2O_2$ treatment of PC12 cells was significantly reduced when methanol extract was present in the media compared to PC12 cells treated with $H_2O_2$ only. In a cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium-bromide (MTT), the methanol extract showed protective effects against $H_2O_2$-induced neurotoxicity, and lactate dehydrogenase (LDH) release into the medium was also inhibited. Furthermore, the inhibitory effect of the methanol extract against acetylcholinesterase was dose-dependent.
Keywords
neuronal cell protection; cheonggukjang; acetylcholinesterase;
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1 Kim DJ, Seong KS, Kim DW, Ko SR, Chang CC. Antioxidative effects of red ginseng saponins on paraquat-induced oxidative stress. J. Ginseng Res. 28: 5-10 (2004)   과학기술학회마을   DOI
2 Ames BN. Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases. Science 221: 1256-1264 (1983)   DOI
3 Yoon MY, Kim JY, Hwang JH, Cha MR, Lee MR, Jo KJ, Park HR. Protective effect of methanolic extracts from Dendrobium nobile Lindle. on $H_{2}O_{2}$ -induced neurotoxicity in PC12 cells. J. Korean Soc. Apple. Biol. Chem. 50: 63-67 (2007)
4 Choi JH, Kim DI, Park SH, Baek SJ, Kim NJ, Cho WK, Kim KJ, Kim HS. Effects of pine needle ethyl acetate fraction on acetylcholine (ACh) and its related enzymes in brain of rats. Korean J. Nutr. 37: 95-99 (2004)   과학기술학회마을
5 Jeong CH, Choi SG, Heo HJ. Analysis of nutritional components and evaluation of functional activities of Sara borealis leaf tea. Korean J. Food Sci. Technol. 40: 586-592 (2008)   과학기술학회마을
6 Lee JJ, Cho CH, Kim JY, Lee DS, Kim HB. Antioxidant activity of substances extracted by alcohol from cheonggukjang powder. Korean J. Microbiol. 37: 177-181 (2001)
7 Zhao B. Natural antioxidants protect neurons in Alzheimer's disease and Parkinson's disease. Neurochem. Res. 34: 630-638 (2009)   DOI   ScienceOn
8 Heo HJ, Kim MJ, Suh YM, Choi SJ, Mun NS, Kim HK, Kim EK, Shin DH. Protective effects of daidzein on oxidative stressinduced neurotoxicity and scopolamine-mediated cognitive defect. J. Food Sci. 70: S91-S94 (2005)
9 Choi HK, Lim YS, Kim YS, Park SY, Lee CH, Hwang KW, Kwon DY. Free-radical-scavenging and tyrosinase-inhibition activities of Cheonggukjang samples fermented for various times. Food Chem. 106: 564-568 (2008)   DOI   ScienceOn
10 Ishida H, Uesugi T, Hirai K, Toda T, Nukaya H, Yokotsuka K, Tsuji K. Preventive effects of the plant isoflavones, daidzin, and genistin, on bone loss in ovariectomized rats fed a calcium-deficient diet. Biol. Pharm. Bull. 21: 62-66 (1998)   DOI   ScienceOn
11 AOAC. Official Methods of Analysis of AOAC Intl. 13th ed. 920.39, 934.01, 942.05, 954.01 and 974.06, Association of Official Analytical Chemists, Washington, DC, USA (1990)
12 Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemical from various cultivars of plums. Food Chem. 81: 321-326 (2003)   DOI   ScienceOn
13 Choi SJ, Yoon KY, Choi SG, Kim DO, Oh SJ, Jun WJ, Shin DH, Cho SH, Heo HJ. Protective effect of Acanthopanax senticosus on oxidative stress induced PC12 cell death. Food Sci. Biotechnol. 16: 1035-1040 (2007)   과학기술학회마을
14 Heo HJ, Cho HY, Hong BS, Kim HK, Kim EK, Kim BK, Shin, DH. Protective effect of 4',5-dihydroxy-3',6,7-trimethoxyflavone from Artemisia asiatica against AB-induced oxidative stress in PC12 cells. Amyloid 8: 194-201 (2001)   DOI   ScienceOn
15 Ellman GL, Doutney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95 (1961)   DOI   ScienceOn
16 Kwak CS, Kim MY, Kim SA, Lee MS. Cytotoxicity on human cancer cells and antitumorigenesis of cheonggukjang, a fermented soybean product, im DMBA-treated rats. Korean J. Nutr. 39: 347-356 (2006)   과학기술학회마을
17 Song HN, Jung KS. Quality characteristics and physiological activities of fermented soybean by lactic acid bacteria. Korean J. Food Sci. Technol. 38: 475-482 (2006)   과학기술학회마을
18 Kim KY, Hahm YT. Recent studies about physiological functions of cheonggukjang and functional enhancement with genetic engineering. J. Genetic Engineer. Res. 16: 1-18 (2003)
19 Hong SW, Kim JY, Lee BK, Chung KS. The bacterial biological response midifier enriched cheonggukjang fermentation. Korean J. Food Sci. Technol. 38: 548-553 (2006)   과학기술학회마을
20 Yang JL, Lee SH, Song YS. Improving effect of powders of cooked soybean and cheonggukjang on blood prossure and lipid metabolism in spontaneously hypertentive rats. J. Korean Soc. Food Sci. Nutr. 32: 899-905 (2003)   과학기술학회마을   DOI
21 Kim YT, Kim WK, Oh HI. General microbiology, physiology and metabolism; Screening and identification of the fibrinolytic bacterial strain from cheonggukjang. Korean J. Microbiol. Biotechnol. 23: 1-5 (1995)
22 Anthony MS, Clarkson TB, Hughes JrCL, Morgan TM, Burke GL. Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. J. Nutr. 126: 43-50 (1996)
23 Heo HJ, Choi SJ, Choi SG, Shin DH, Lee JM, Lee CY. Effects of banana, orange, and apple on oxidative stress-induced neurotoxicity in PC12 cells. J. Food Sci. 73: 28-32 (2008)   DOI   ScienceOn
24 Merz-Demlow BE, Duncan AM, Wangen KE, Xu X, Carr TP, Phipps WR, Kurzer MS. Soy isoflavones improve plasma lipids in normocholesterolemic, premenopausal women. Am. J. Clin. Nutr. 71: 1462-1469 (2000)
25 Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JrJW. Soy protein and isoflavones; Their effects on blood lipids and bone density in postemenopausal women. Am. J. Clin. Nutr. 68: 1375-1379 (1998)
26 Ames BN, Shigenaga MK, Hagen TM. Oxidant, antioxidants, and the degenerative disease of aging. P. Natl. Acad. Sci. USA 90: 7915-7922 (1993)   DOI   ScienceOn
27 Yoon MY, Lee BB, Kim JY, Kim YS, Park EJ, Lee SC, Park HR. Antioxidant activity and neuroprotective effect of Psoralea corylifolia Linne extracts. Korean J. Pharmacogn. 38: 84-89 (2007)   과학기술학회마을
28 Kim SH, Yang JL, Song YS. Physiological functions of cheonggukjang. Food Indus. Nutr. 4: 40-46 (1999)
29 Lee BY, Kim DM, Kim KH. Physico-chemical properties of viscous substance extracted from cheonggukjang. Korean J. Food Sci. Technol. 23: 599-604 (1991)
30 Kim JS. Current research trends on bioactive function of soybean. Korea Soybean Digest 13: 17-24 (1996)
31 Lee YS. Antioxidative and physiological activity of extracts of Angelica dahurica leaves. Korean J. Food Preserv. 14: 78-86 (2007)   과학기술학회마을
32 Kim WK, Choi KH, Kim YT, Park HH, Choi JY, Lee YS, Oh HI, Kwon IB, Lee SY. Purification and characterization of a fibrinolytic enzyme produced form Bacillus sp. strain CK 11-4 screened from cheonggukjang. Appl. Environ. Microbiol. 62: 2482-2488 (1996)
33 Sato T, Yamada Y, Ohtani Y, Mitsui N, Murasawa H, Araki S. Efficient production of menaquinone by minadione resistant mutant of Bacillus subtilis. J. Ind. Microbiol. Biot. 26: 115-120 (2001)   DOI   ScienceOn
34 Sagara Y, Dargusch R, Chambers D, Davis J, Schubert D, Mater P. Cellular mechanisms of resistance to chronic oxidative stress. Free Radical Bio. Med. 24: 1375-1389 (1998)   DOI   ScienceOn