• Title/Summary/Keyword: cross resistance

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Inheritance of Tetradifon Resistance in Two-spotted Spider Mite (Acari: Tetranychidae) and Its Cross Resistance (Tetradifon에 대한 점박이응애(Tetranychus urticae Koch) 저항성의 유전양식과 교차저항성)

  • 박정규;이상계;최병렬;유재기;이정운
    • Korean journal of applied entomology
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    • v.35 no.3
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    • pp.260-265
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    • 1996
  • A field colony of the two-spotted spider mite, Tetranychus urticae Koch, was selected with tetradifon for 4 mousing whole-plant residual method. This strain showed 371-fold resistance to tetradifon. The log dosehatchability lines of $F_{1}$ eggs(RS cross; Td5female$\times$Smale, and SR cross; Sfemale$\times$Td5male) were closer to the line of the resistant colony than to that of the susceptible. These differences could be due to cytoplasmic inheritance or maternal effect. The estimate of dominance index (D) for the $F_{1}$ eggs of RS cross was 0.998 and that for $F_{1}$ eggs of SR cross was 0.262. This indicates that tetradifon resistance is completely dominant in RS cross and incompletely dominant in SR cross. Td5 strain exhibited high levels of resistance to clofentezine, benzoximate, and chlorfencon, and no cross resistance to fenazaquin, pyridaben, flufenoxuron, tebufenpyrad, and fenothiocarb.

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Inheritance if Resistance in Dicofol-selected Strain of the Two-spotted Spider Mite, Tetranychus urticae Koch (Acarina: Tetranychidae), and its Cross Resostance (Dicofol 저항성 점박이응애의 저항성 유전과 교차저항성)

  • Kim, Gil-Hah;Song, Cheol;Park, No-Joong;Cho, Kwang-Yun
    • Korean journal of applied entomology
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    • v.33 no.4
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    • pp.230-236
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    • 1994
  • Dicofol susceptible two-spotted spider mite(S) which was selected by 150 times of dicofol treatment showed 27 5-fold increase in resistance as compared to S strain. Inheritance of resistance and crossresistance in selected strain($R_d$) were investigated. There were differences oi susceptibility in the dicofol concentmtion-moltaliity relationships between $F_1$ progenies (R♀, XS♂, R♂, XS♀) obtained from reciprocal cross with the S strain and $R_d$, strain. Degree of dominance of the $F_1$ progenies (R♀, XS♂, R♂, XS♀) were -0.29 and -0.72 in adult females and -0.42 and -0.93 in adult males, respectively. These results suggest that inheritance of dicofol resistance is controlled by an incomplete recessive. $R_d$ strain exhibited a high level of cross resistance to amitraz, and also showed cross resistance to pyrethrotd acaricides, acrinathrin and bifenthnn. $R_d$ strain showed non-cross reslstancz to abamectin, chlolfenson, clofentezine, cyhexatm, fenbutatin oxlde, fenpyroximate, hexythiazox, monocrotophos, propargite and tetradifon, but negatively correlated cross-resistance to azocyclotin and fenbutatin oxide belonging to tin acaricides.

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Resistance development and cross-resistance of green peach aphid, Myzus persicae (Homoptera : Aphididae), to imidacloprid (Imidacloprid에 대한 복숭아혹진딧물의 저항성 발달 및 교차저항성)

  • Choi, Byeong-Ryeol;Lee, Si-Woo;Yoo, Jai-Ki
    • The Korean Journal of Pesticide Science
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    • v.6 no.4
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    • pp.264-270
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    • 2002
  • Studies on the resistance monitoring of green peach ahpid, Myzus persicae, its development pattern by artificial selection with imidacloprid and cross-resistance were carried out to develope resistance management strategy. Resistance ratios of M. persicae collected at Hwachon and Dunnae among 5 locations in alpine cultivation area appeared to be high as 37.2 and 16.5, respectively. Resistance of aphid to imidacloprid developed slowly up to 20 time selection, and after that it grew quickly. Imidacloprid-resistant aphid strain showed low cross-resistance ratios(<10) to most of organophosphates, carbamates, and mixed insecticides except pirimicarb(487.8), but high ratios to acetamiprid(143.0) which is one of the neonicotinoids like imidacloprid, and pyrethroids such as deltamethrin(14.9), flucythrinate(12.9) and halothrin(15.9).

Molecular Mechanism of ABC Transporter Mdr49A Associated with a Positive Cross-Resistance in Transgenic Drosophila (형질전환 초파리를 이용한 Mdr49A 유전자의 살충제 교차저항성 기능 구명)

  • Seong, Keon Mook;Pittendrigh, Barry R.
    • Korean journal of applied entomology
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    • v.59 no.4
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    • pp.341-348
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    • 2020
  • The ATP-binding cassette (ABC) transporter superfamily represents the largest transmembrane protein that transports a variety of substrates across extra- and intra-cellular membranes. In insects, the ABC transporter proteins play crucial roles in insecticide resistance. To date, no studies have investigated the involvement of ABC transporter gene for cross-resistance to insecticide chemistries. Here, we studied such possible mechanisms against six conventional insecticides using transgenic Drosophila melanogaster strains carrying Mdr49 transcript variant A. For the 91-R and 91-C strains of Drosophila melanogaster, although they have a common origin, 91-R has been intensely selected with DDT for over 60 years, while 91-C has received no insecticide selection. Our transgenic analyses showed that overexpression of 91-R-MDR49 transcript variant A along with three amino acid variations can yield a relatively low degree of cross-resistance to carbofuran (2.0~6.7-fold) and permethrin (2.5~10.5-fold) but did not show cross-resistance to abamectin, imidacloprid, methoxychlor, and prothiofos as compared to the Gal4-driver control strain without transgene expression. These results indicate that the overexpression of Mdr49A in itself leads to a cross-resistance and three amino acid changes have additional effects on positive cross-resistance to carbofuran and permethrin.

Inheritance and Stability of Etoxazole Resistance in Twospotted Spider Mite, Tetranychus urticae, and Its Cross Resistance (점박이응애의 Etoxazole저항성 유전과 안전성 및 교차저항성)

  • 이소영;안기수;김철수;신상철;김길하
    • Korean journal of applied entomology
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    • v.43 no.1
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    • pp.43-48
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    • 2004
  • Development of 3,700 folds resistance to etoxazole was found in the population of twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Buyo, Chungnam Provience in August 2000. This population was selected for 3yr with etoxazole to get 5,000,000 folds increase in resistance as compared to susceptible (S) strain. The etoxazole resistance was stablized for 16 months under the condition of no acaricide application. Inheritance and cross resistance in etoxazole to some acaricides of the etoxazole resistance strain (R) were investigated. There were differences of susceptibility in the etoxazole concentration-mortality relationships between $F_1$, $F_2$ progenies obtained from reciprocal cross with the S and R strains (R$_{♂}$${\times}$S$_{♂}$${\times}$R$_{♂}$). Degrees of dominance were 0.98 and 0.98 in $F_1$ and $F_2$ progenies of R$_{♂}$${\times}$S$_{♂}$, and -0.97 and -0.68 in $F_1$ and $F_2$ progenies of S$_{♀}$${\times}$R$_{♂}$ respectively. Inheritance in $F_1$ and $F_2$ progenies of R$_{♀}$${\times}$S$_{♂}$ were complete dominant. However $F_1$ and $F_2$ progenies of S$_{♀}$${\times}$R$_{♂}$ were incomplete recessive. These results suggest that inheritance of etoxazole resistance is controlled by a complete dominance. The R strain exhibited cross resistance to acequinocyl and emamectin benzoates in adult females, and milbemectin, amitraz and pyridaben in eggs. However they showed negatively correlated cross-resistance to bifenazate, a carbazate acaricide. These results may indicate bifenazate could be useful for the control of etoxazole resistant T. urticae population.

Fungicide Resistance and Genetic Diversity of Botrytis cinerea of Citrus (감귤 잿빛곰팡이병균의 살균제에 대한 저항성 및 유전적 다양성)

  • 고영진;이재군;서정규;문두길;한해룡
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.682-688
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    • 1998
  • Fungicide resistance of 48 isolates of Botrytis cinerea collected from citrus in Cheju was investigated and genetic diversity was analyzed with random amplified polymorphic DNA(RAPD). High levels of resistance to benzimidazole fungicides benomyl and thiophanate-methyl and N-phenylcarbamate fungicide diethofencarb were observed. Negative cross resistance was clear between benzimidazole and N-phenylcarbamate fungicides, and multiple resistance to the fungicides was also observed. There was cross resistance among the dicarboximide fungicides procymidione, vinclozolin and iprodione as it was observed between the benzimidazole fungicides benomyl and thiophanate-methyl. The lowest levels of resistance were to the dicarboximide fungicides, but no sensitive isolate to polyoxin B was observed. The isolates showed genetically diverse RAPD profiles according to the geographic origin collected, but there was no significant correaltion between RAPD profiles of genomic DNA and the levels of fungicide resistance of the isolates. The isolates showed genetically diverse RAPD profiles, indicating that genetic differentiation had already occurred in the populations of B. cinerea distributed in Cheju.

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Fatigue damage detection of CFRP using the electrical resistance change method

  • Todoroki, Akira;Mizutani, Yoshihiro;Suzuki, Yoshiro;Haruyama, Daichi
    • International Journal of Aeronautical and Space Sciences
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    • v.14 no.4
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    • pp.350-355
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    • 2013
  • Electrical resistance change measurements were performed, to detect fatigue damage of a quasi-isotropic CFRP and cross-ply CFRP laminates. A four-probe method was used to measure the exact electrical resistance change. A three-probe method was used to measure the electrical contact resistance change, during long cyclic loading. The specimen side surface was observed using a video-microscope to detect damage. The measured electrical resistance changes were compared with the observed damage. The results of this study show that the electrical resistance increase of the quasi-isotropic laminate was caused by a delamination crack between ${\pm}45^{\circ}$ plies. Matrix cracking caused a small electrical resistance increase of the cross-ply laminate, but the decreased electrical resistance caused by the shear-plastic deformation impedes matrix-cracking detection.

Inheritance and Cross Resistance of Bifenazate Resistance in Twospotted Spider Mite, Tetranychus urticae (Bifenazate 저항성 점박이응애의 유전과 교차저항성)

  • Yu Jeong-Soo;Seo Dong-Kyu;Kim Eun-Hee;Han Jong-Been;Ahn Ki-Su;Kim Gil-Hah
    • Korean journal of applied entomology
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    • v.44 no.2
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    • pp.151-156
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    • 2005
  • The development of resistance to bifenazate (resistance ratio of egg=40.3 folds) was found in population of the twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Chilgok, Gyeongbuk Province in December 2000. This population was selected for 4 years with bifenazate treatment (over 150 times), and showed 248.8 folds increase in resistance as compared to susceptible (S) strain. Inheritance of bifenazate resistant strain (R) and cross resistance of this strain to 9 acaricides were investigated. There were differences of susceptibility in the bifenazate concentration-mortality relationships in F1 progenies obtained from reciprocal cross with the S and R strain $(R{\times}S,\;S{\times}R)$. Degrees of dominance were 0.48 and 0.94 in adult females and eggs of $R{\times}S$ and -0.85 and -0.17 in adult females and eggs of $S{\times}R$, respectively. Inheritance type in the F1 progeny of $R{\times}S$ was incomplete dominant, and F1 progeny of $S{\times}R$ was incomplete recessive. These results suggest that inheritance of bifenazate resistance is controlled by a complete dominance. The R strain exhibited cross resistance to acequinocyl and fenpyroximate in adult females, and amitraz, emamectin benzoate, fenpyroximate, milbemectin, pyridaben and spirodiclofen in eggs. However they showed negatively correlated cross-resistance to emamectin benzoate and milbemectin in adult females, and abamectin in eggs.

Bacterial Resistance to LB20304, a New Fluoroquinolone Antibiotic

  • Kim, Mu-Yong;Paek, Kyoung-Sook;Kim, In-Chull;Kwak, Jin-Hwan
    • Archives of Pharmacal Research
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    • v.19 no.5
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    • pp.400-405
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    • 1996
  • In vitro studies were conducted to dertermine the frequency rate of spontaneous resistance to LB20304 and to dertermine whether cross-resistance to other antimicrobial agents develops. In eight strains of bacteria, the frequency of mutation to LB20304 at the concentrations of four and eight times the minimal inhibitory concentration(MIC) ranaged from less than 4.0 ${\times}$ $10^{-10}$ to 2.2 $\{times}$ $10^{-10}$ . These results were similar to those founf for other new fluoroquinolones. THe development of stepwise resistance was determined by repeated subculture in broth in the presence of increasing concentration of the compounds. Exposure of bacteria to increasing concentrations of LB20304 resulted in the selection of organisms with higher MICs. There were 4- to 128-fold increases in the MIC of LB20304 for bacterial strains of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli and Pseudomonas aeruginosa. However, those strains selected after repeated exposure were well within the susceptibility range for LB20304 except for Pseudomonas aeruginosa. The resistant isolates selected with LB20304 showed cross-resistance when tested against ciprofloxacin and vice versa.

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Effect of Resistance Band Exercise on Body Composition, Physical Fitness and Health Promoting Behavior in Nursing Students (탄성밴드운동이 간호대학생의 체성분, 체력과 건강증진행위에 미치는 효과)

  • Baek, Hee Chong;Lee, Suk Jeong;Shin, Gisoo;Lim, Eun Ju
    • Journal of Korean Public Health Nursing
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    • v.28 no.2
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    • pp.310-319
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    • 2014
  • Purpose: The purpose of this study is to examine the effects of a resistance band training program on body composition, physical fitness, and health promotion behavior in nursing students. Method: This study has a one-group pretest-posttest design to examine the change in body composition, physical fitness, and health-promoting lifestyle profile after participation in the resistance band training program. Training consisted of hands-based exercises using an elastic resistance band for ten weeks. Participants performed the resistance band training two days per week with a sports trainer, and 30 minutes self-training three days per week. The participants were 21 freshmen in nursing. Results: Mean age of the participants was 20.9 years, and 61.9% were not satisfied with their body shape. After the training program, muscle mass, muscular endurance, and balance of the body were significantly improved. In health promoting lifestyle profile, categories of physical activity, health responsibility, and stress management showed a significant increase. Conclusion: Results of this study suggested that exercising with a resistance band improved body composition, physical fitness, and health promoting behavior in the nursing students. We propose that resistance band training should be suitable for those who have the environment with limitation of time and space.