• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.2
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• pp.112-119
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• 2011

Purpose: Collagen membranes are used extensively as bioabsorbable barriers in guided bone regeneration. However, collagen has different effects on tissue restoration depending on the type, structure, degree of cross-linking and chemical treatment. The purpose of this study was to evaluate the inflammatory reaction, bone formation, and degradation of dehydrothermal treated porcine type I atelocollagen (CollaGuide$^{(R)}$) compared to of the non-crosslinked porcine type I, III collagen (BioGide$^{(R)}$) and the glutaldehyde cross-linked bovine type I collagen (BioMend$^{(R)}$) in surgically created bone defects in rat mandible. Methods: Bone defect model was based upon 3 mm sized full-thickness transcortical bone defects in the mandibular ramus of Sprague-Dawley rats. The defects were covered bucolingually with CollaGuide$^{(R)}$, BioMend$^{(R)}$, or BioGide$^{(R)}$ (n=12). For control, the defects were not covered by any membrane. Lymphocyte, multinucleated giant cell infiltration, bone formation over the defect area and membrane absorption were evaluated at 4 weeks postimplantation. For comparison of the membrane effect over the bone augmentation, rats received a bone graft plus different covering of membrane. A $3{\times}4$ mm sized block graft was harvested from the mandibular angle and was laid and stabilized with a microscrew on the naturally existing curvature of mandibular inferior border. After 10 weeks postimplantation, same histologic analysis were done. Results: In the defect model at 4 weeks post-implantation, the amount of new bone formed in defects was similar for all types of membrane. Bio-Gide$^{(R)}$ membranes induced significantly greater inflammatory response and membrane resorption than other two membranes; characterized by lymphocytes and multinucleated giant cells. At 10 weeks postoperatively, all membranes were completely resorbed. Conclusion: Dehydrotheramal treated cross-linked collagen was safe and effective in guiding bone regeneration in alveolar ridge defects and bone augmentation in rats, similar to BioGide$^{(R)}$ and BioMend$^{(R)}$, thus, could be clinically useful.

• Journal of Microbiology
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• v.42 no.1
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• pp.25-31
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• 2004

Sf9 cells have obvious advantages for the conventional production technology of vaccine. They are useful tools for high concentration and large-scale cultures. Sf9 cells were grown to maximal concentration, 8${\times}$l0$\^$6/ cells/$m\ell$ in a 500$m\ell$ spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing-l strain, Sf9 cells were infected with the virus. By 4-5 days post-infection, 10-15 % of the Sf9 cells showed cytopathic effect (CPE), from granularity to the formation of syncytia and multinucleated giant cells continuously observed over a period of 35 days. Positive fluorescent reactions were detected in 30-40% of cells infected with the JE virus Beijing-l strain, and the uninfected Sf9 cells were completely negative. Virus particles, propagated in Sf9 and Vero cells, were concentrated by sedimentation on 40% trehalose cushions by ultracentrifugation, and showed identical patterns of viral morphogenesis. Complete virus particles, 40 to 50 nm in diameter, were observed, and JE virus envelope (E) proteins, at 53 kDa, were found in the western blot analysis to the anti-JE virus E protein monoclonal antibody and reacted as a magenta band in the same position to the glycoprotein staining. To evaluate whether the infectious virus was produced in Sf9 cells inoculated with the JE virus Beijing-l stain, Sf9 cells were inoculated with the virus, and sample harvested every 5 days. The titers of the JE virus Beijing-l strain rose from 1.0${\times}$l0$\^$5/ to 1.5${\times}$l0$\^$6/ pfu/$m\ell$. The infected Sf9 cells could be subcultured in serum-free medium, with no change in the plaque sizes formed by the JE virus Beijing-l strain in the plaque assay. It is suggested that the ability of the JE virus Beijing-l strain to infect Sf9 cells in serum-free media will provide a useful insect cell system, where the JE virus replication, cytopathogenicity and vaccine immunogen can be studied.

• Wind and Structures
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• v.21 no.1
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• pp.41-61
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• 2015

The flutter stability of long-span suspension bridges during erection can be more problematic and more susceptible to be influenced by many factors than in the final state. As described in this paper, numerical flutter stability analyses were performed for the construction process of Zhongdu Bridge over Yangtze River using the commercial FE package ANSYS. The effect of the initial wind attack angle, the sequence of deck erection, the stiffness reduction of stiffening girders, the structural damping, and the cross cables are discussed in detail. It was found that the non-symmetrical sequence of deck erection was confirmed to be aerodynamically favourable for the deck erection of long-span suspension bridges and the best erection sequence should be investigated in the design phase. While the initial wind attack angle of $-3^{\circ}$ is advantageous for the aerodynamic stability, $+3^{\circ}$ is disadvantageous compared with the initial wind attack angle of $0^{\circ}$ during the deck erection. The stiffness reduction of the stiffening girders has a slight effect on the flutter wind speed of the suspension bridge during erection, but structural damping has a great impact on it, especially for the early erection stages.

• Korean Journal of Oriental Medicine
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• v.14 no.1
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• pp.85-89
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• 2008

This study is to investigate cardiotonic effect of acupuncture on heart rate variability(HRV) analyzed by a nonlinear way(DFA, Detrended Fluctuation Analysis). It was designed as a randomized, single-blind, waiting list-controlled, cross-over study. We assessed heart rate and R-R intervals in Circadian electrocardiography with a Holter monitoring device for twelve hospitalized participants. The compatible analytical program, Zymed, was used for generating the signals of R-R intervals from 24 hour-ECG. In DFA analysis, we produced DFA alpha 1, alpha 2 parameters according to the process of Cygwin module of Linux server. We tested if there was any difference between HRV parameters using SPSS, a statistical package. There was no difference between acupuncture and no treatment group in DFA alpha 2 parameter {95% Confidence Interval (-)0.058 - 0.037, P = .565}. Two group all showed large intra-individual variations. Consequently, acupuncture treatment did not modulate the complexity of HRV in a DFA analysis. This study can be a rationale for acupuncture's properties on cardiovascular and autonomic systems.

• Journal of Power System Engineering
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• v.16 no.6
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• pp.30-37
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• 2012

Effect of rib heights is found as significant parameter to enhance convective heat transfer performance under laminar and low turbulent regime. Circular ribs with different ribheight to channel height ratios, e/H = 0.05, 0.1, 0.15, are fabricated over the copper substrate respectively in a rectangular duct having 7.5 cross sectional aspect ratio. Only one rib pitch to rib height ratio (P/e = 10) has been chosen for all different height ribs. The result shows that the arithmetic average of turbulence intensity decreases with decreasing roughness height calculated between two ribs under laminar and low turbulent region. It occurs because the area of recirculation and reattachment zone also decreases with decreasing rib height. Optimum thermal enhancement factor is derived by 0.1 rib height to channel height ratio under low turbulent region but 0.15 rib height to channel height ratio gives maximum subjected to laminar flow.

• Environmental Analysis Health and Toxicology
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• v.16 no.4
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• pp.211-221
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• 2001

The purpose of this study was to investigate the acute (4 hours) and repeated-dose (6 hours a day, 5 days a week, 4 weeks) toxic effects of 1-hexene on Sprague-Dawley (SD) rats which were treated by inhalation. The results were as follows; 1. The median lethal concentration(LC$_{50}$) was estimated 52,694 ppm (confidence limit 95％; 49,494~55,447 ppm) in acute inhalation. Abnormal clinical signs related to the 1-Hexene were not observed with the acute inhalation dose. Cross findings of necropsy revealed on evidence of specific toxicity related to the 1-hexene. II. By repeated inhalation exposure the body weight of male were more or less reduced by the dose of 2,500 ppm and 5,000 ppm compared with control group. However there were no significant variation hematology and blood biochemistry for the exposed rats compared with the control rats. Abnormal clinical signs and gross findings of necropsy related to the 1-hexene were not shown. In conclusion when we exposed 1-hexene to SD rats for 4 weeks, 5 days per week, 6 hours per day, the Lowest observed effect level (LOEL) was over 2,500 ppm and Non observed effect level (NOEL) was below 500 ppm.

• Steel and Composite Structures
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• v.2 no.4
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• pp.297-314
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• 2002

An energy method of analysis is presented which can be used to study the inelastic lateral-distortional buckling of hot-rolled I-sections continuously restrained at the level of the tension flange. The numerical modelling leads to the incremental and iterative solution of a fourth-order eigenproblem, with very rapid solutions being obtainable, so as to enable a study of the factors that influence the strength of continuously restained I-beams to be made. Although hot-rolled I-sections generally have stocky webs and are not susceptible to reductions in their overall buckling loads as a result of cross-sectional distortion, the effect of elastic restraints, particularly against twist rotation, can lead to buckling modes in which the effect of distortion is quite severe. While the phenomenon has been studied previously for elastic lateral-distortional buckling, it is extended in this paper to include the constitutive relationship characteristics of mild steel, and incorporates both the so-called 'polynomial' and 'simplified' models of residual stresses. The method is validated against inelastic lateral-torsional buckling solutions reported in previous studies, and is applied to illustrate some inelastic buckling problems. It is noted that over a certain range of member slenderness the provisions of the Australian AS4100 steel standard are unconservative.

• Physical Therapy Korea
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• v.17 no.2
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• pp.67-74
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• 2010

The purpose of this study was to examine the acute effects of nerve mobilization, static stretching, and hold-relax on the flexibility of hamstring muscles and their surface electromyographic (EMG) responses to passive stretches in poststroke hemiparesis. This study was a randomized cross-over trial. Fourteen subjects received three treatment sessions in random order with each consisting of ten repetitions. The treatment sessions included nerve mobilization, static stretching, and hold-relax. The treatment sessions were held at least 24 hours apart to minimize any carryover effect. The outcome was measured by the distance between the greater trochanter and lateral malleolus and hamstring EMG activity during passive knee extension stretching. Repeated-measures analysis of variance showed significant changes in hamstring flexibility and EMG activity in main effect of time pre, post and followup (p<.05). However, no significant differences occurred among the three stretching techniques. No technique was consistently found to be superior. The three stretching techniques in this study make it difficult to determine the most effective technique. Therefore, clinicians use nerve mobilization of effective stretching techniques with other stretching techniques.

• Korean Journal of Adult Nursing
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• v.20 no.5
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• pp.756-764
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• 2008

Purpose: The purpose of this study was to examine the effects of aromatherapy on dysmenorrhea, menstrual pain, anxiety, and depression in female college students. Methods: A two-group cross-over design was used. The sample included a total of 20 female students who were enrolled in a 3 year nursing program. The treatments(aroma essential oil inhalation and the placebo inhalation) were given using a necklace. The data were analyzed by $x^2$-test, paired t-test, and Mann-Whitney test using SPSS/WIN 12.0 program. Results: The level of dysmenorrhea after the aroma treatment was not significantly different from the dysmenorrhea levels at pre-test and after the post-placebo treatment. The menstrual pain, anxiety and depression significantly improved after the aroma treatment. However, the levels of pain, anxiety, and depression after the aroma treatment were not significantly different from those after the placebo treatment. Conclusion: Aroma inhalation may be a effective in managing menstrual pain, anxiety, and depression. In order to further clarify the effect of aroma treatment on discomfort during menstruation, replication studies are necessary. Future studies need to examine the effects of different types of essential oils, administrating methods, and the lasting time of aroma treatment effect.

• Journal of Life Science
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• v.7 no.1
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• pp.59-65
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• 1997

In this experiment, gene actions were analysized for seven barley parents in order to obtain basic information on their genetic improvement by diallel crosses. The results obtained were summarized as follows. In analysis of variance, additive, dominant, maternal and reciprocal effects were observed significantly for culm length, tiller number, number of spiked per plant, culm diameter, awn length, leaf width, number of grains per spike, and 1000 grain weight. Over-dominance was shown by Vr-Wr graphic analysis in five characters such as tiller number, number of spikes per plant, length of flag leaf, number of grains per spike, and 1000 grain weight, and partial dominance in four characters such as culm length, culm diameter, awn length, and leaf width. Component of genetic varience analyzed for four characters such as culm diameter, awn length, length of flag leaf, and leaf width showed that additive effects were higher than dominant effects. Culm length, tiller number, number of spikes per plant, number of grains per spike, and 1000 grain weight showed dominant effects higher than additive effects. The narrow-sense heritability for awn length, leaf width, and number of grains per spike showed high values as more than 0.62, while broad-sense heritability for culm length, number of spikes per plant, culm diameter, awn length, leaf width, number of grains per spike, and 1000 grain weight showed high values as more than 0.65.