• 한국육종학회지
• /
• 제40권4호
• /
• pp.414-421
• /
• 2008

We conducted a QTL analysis of agronomic traits using 117 $BC_3F_5$ and $BC_3F_6$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_5$ and $BC_3F_6$ lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.

• 한국육종학회지
• /
• 제41권2호
• /
• pp.168-172
• /
• 2009

'풍한'은 조숙 다수 월동가능 조사료용 귀리 품종을 육성할 목적으로 1999년에 조숙인 '식용귀리'와 대립종인 'PA202-210'을 교배한 $F_1$잡종에 내한성 계통인 'Beltsville 61-150'을 부본으로 인공교배하여 집단 및 계통육종법에 의해 계통을 선발한 후 2004년부터 생산력검정시험 및 지역적응시험을 거쳐 2008년에 육성된 품종으로 주요 특성은 다음과 같다. 1. '풍한'은 잎폭이 중 정도이며, 이삭형은 산수형이고, 종자색은 황백색이다. 2. '풍한'은 적응지역에서 출수기가 평균 5월 7일이며, 출수 후 20일에 평가한 생초수량이 ha당 평균 50.1 톤, 건물수량이 평균 14.5 톤으로서 기존 품종인 '삼한'보다 출수기가 2일 빠르고 조사료 수량도 높다. 3. '풍한'의 조사료 품질은 조단백질 함량이 6.7%, TDN이 60.3%로 표준품종인 '삼한귀리'와 대등하다.

• 한국동물위생학회지
• /
• 제32권3호
• /
• pp.219-225
• /
• 2009

A cross sectional survey was conducted to determine the seroprevalence of brucellosis in cattle in Bangladesh Agricultural University (BAU) Veterinary Clinics, in BAU Dairy Farm and Vabokhali from June 2008 to November 2008. A total of 200 serum samples were collected from BAU Veterinary Clinic, from BAU Dairy Farm and Vabokhali. Among the serum samples 143 sera samples were collected from BAU Veterinary Clinic, 42 serum samples from BAU Dairy Farm and 15 serum samples from Vabokhali. Sera were separated from blood samples and tested with specific Brucella abortus antigen (BAA) test and B. melitensis antigen (BMA) test. The overall seroprevalence of brucellosis in cattle was 5% in BAA and 0.5% in BMA. It was observed that, a significant higher prevalence of B. abortus was found in female than male. An insignificant higher prevalence of brucellosis was found in adult cattle (aged above 5 years), in cross breed cattle, in cattle with grazing, cattle breed by natural breeding, and in pregnant cows. Although insignificant but a higher prevalence of brucellosis was found in aged cattle than young cattle, cross bred cattle, pregnant cattle than non pregnant cattle, cattle with grazing. A higher prevalence of brucellosis was found in female cattle than male.

• 한국약용작물학회지
• /
• 제16권5호
• /
• pp.287-293
• /
• 2008

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about $17.0\;mm{\times}4.0\;mm$ and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.

• Journal of Plant Biotechnology
• /
• 제7권1호
• /
• pp.27-35
• /
• 2005

Eighty-five different cultivars of Brassica rapa, B. juncea, B. nap us, B. carinata, B. oleracea and hexaploid Brassica collected from Bangladesh, Japan, China and Denmark were analyzed by SDS-PAGE for seed and leaf protein variations, using esterase, acid phosphatase and peroxidase isozyme analysis. Ten polymorphic bands were identified from seed protein however no identifiable polymorphic band was found in the leaf protein. Polymorphic markers clearly distinguished the different Brassica species as well as yellow sarson (YS) and brown seeded (BS) cultivars of B. rapa. The $F_1$ cross between YS and brown seeded cultivars showed the existance of all poly-morphic bands of the respective parents. The Bangla-deshi and Japanese cultivars of B. rapa differed in the amount of seed protein. In the case of isozyme analysis, esterase showed the highest number of polymorphic bands (13) followed by acid phosphatase (9) and peroxidase (5). These polymorphic markers were very effec-tive for classification of all the species studied in this experiment. In parentage tests using isozymes, the hybridity of intra-and-interspecific crosses of almost all the seedlings could be identified from their respective cross combinations. Esterase polymorphism showed a clear differentiation between YS and BS types of B. rapa. In addition, two esterase polymorphic markers were iden ified to differentiate some cultivars of B. juncea. Segregation patterns in these two esterase bands showed a simple Mendelian monohybrid ratio of 3:1 in $F_2$, 1:1 in test cross and 1:0 in back cross progenies. No polymorphic band was identified to distinguish different cultivars of the same species by acid phosphatase or peroxidase. Polymerase Chain Reaction (PCR) was carried out with seed coat color specific marker of B. juncea. The yellow seeded cultivars produced a strong band at 0.5 kb and weak band 1.2 kb. In the addition of these two specific bands, Japanese yellow-seeded cultivars expressed two more weak bands at 1.0 kb and 1.1 kb. Where the brown seeded cultivars generated a single strong band at 1.1 kb. In segregating population, the yellow seed coat color marker segregated at a ratio 15 (brown) : 1 (yellow), indicating the digenic inheritance pattern of the trait.

• 한국육종학회지
• /
• 제41권3호
• /
• pp.213-219
• /
• 2009

본 연구는 나리의 종간잡종 육성체계를 확립하는데 필요한 기초자료를 얻기 위하여 OA hybrid 중 자발적으로 생식세포 염색체가 배수화된 계통을 이용하여 후대 획득과 후대검정에 필요한 실험을 수행하여 얻은 결과를 요약하면 다음과 같다. 2n 화분을 생산하는 OA hybrid의 경우 화분의 채취시기에 따른 생존율과 발아율은 10월에 비해 6월이, 생존율은 n성 화분이, 발아율은 2n성 화분이 높았다. 2n 화분을 생산하는 2배체 OA hybrid와 2배체 또는 4배체 Asiatic 또는 Oriental hybrid간 교배시 모본과 부본을 달리함에 따라 후대 획득 수에 차이가 있었는데 OA hybrid를 부본으로 이용했을 때, 상대친을 Asiatic hybrid로 교배했을 때 후대획득이 용이하였다. 즉 A$\times$OA교배에서 후대 획득률이 0.64개체로 O$\times$OA교배에서의 후대 획득률 0.42개체보다 높았으며, OA$\times$A간 교배에서 후대 획득률이 0.40개체인 반면 OA$\times$O교배에서는 전혀 후대획득이 불가능하거나 극히 낮아 후대획득이 어려웠으며, 아울러 2n 화분을 생산하는 OA hybrid의 후대생산을 위한 상대친으로 4배체보다는 2배체가 효과적이었다. 2n 화분을 생산하는 OA hybrid와 Asiatic 또는 Oriental hybrid간 정역교배 결과, OA hybrid를 부본으로 교배했을 때, 상대친으로 Asiatic hybrid을 교배했을 때 후대획득이 용이하였다. 자발적인 2n 화분을 생산하는 2배체 OA hybrid의 여교잡 후대의 ploidy level의 측정결과, OA$\times$A교배에서는 3배체, AA$\times$OA, OO$\times$OA교배에서는 4배체임을 확인할 수 있었으나, O$\times$OA교배에서는 2배체, A$\times$OA교배에서는 2배체 또는 3배체로 확인되었다.

• Molecules and Cells
• /
• 제26권2호
• /
• pp.146-151
• /
• 2008

The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.

• 원예과학기술지
• /
• 제30권6호
• /
• pp.776-779
• /
• 2012

신품종 '여홍'은 국립원예특작과학원에서 1993년 '홍옥'에 '후지'를 인공교배하였고, 2002년에 고품질 과실의 우량 계통에서 예비선발하였다. 4년간 5개 지역에서 지역적응시험을 거쳐 '원교 가-31호'로 2007년에 최종 선발하였다. 과실의 적숙기는 9월 하순이고 과형은 편원형, 과피는 홍색, 바탕은 녹황색, 과육은 황백색이다. 과중은 평균 278g이며, 가용성고형물 함량은 $14.3^{\circ}Brix$, 산 함량은 0.4%로 당산미가 조화로와 식미가 우수하였다. 상온저장력은 3주 정도이며, 탄저병에 이병성이다. 신품종 '여홍'은 '홍로'와 '쓰가루' 품종에는 교배친화성이 있으나, 자방친인 '후지'와는 교배친화성이 없었고, 수세는 약한 편이다.

• 한국작물학회지
• /
• 제31권1호
• /
• pp.74-77
• /
• 1986

단순열성 유전자에 의한 GMS민 IR36ms에 가야벼, 남풍벼, 신광벼 및 수원 296 등 4개 한국 수도품종을 각각 4회 연속 Bulk로 교잡시킨 BC$_3$F$_2$에서 웅성불임 개체를 얻어서 여기에 각 반복친을 1회 더 Backcross시킨 BC$_4$F$_2$세대에서 웅성불임 개체들을 선별하였다. 여기에 각 반복친을 교잡시켜 4품종의 Msms 유전자형을 얻고 이를 다시 BC$_4$F$_2$에서 선발한 웅성불임 개체(msms)에 교잡시키므로서 가야벼ms, 남풍벼ms, 신광벼ms 및 수원 296ms를 육성하였다. 이들 4개 웅성불임 계통들의 주당수수, 수당영화수, 출수기 및 수장은 모품종과 차이가 있었으나 웅성불임 계통들의 간장은 모품종에 비하여 단축되었다. 동일한 웅성불임 유전자를 가진 GMS 계통도 반복친에 따라 자연교잡 정도의 현저한 차이가 있었다. 가야벼ms는 11.5%, 남풍벼 ms는 13.1%, 신광벼ms는 1.9% 그리고 수원 296ms는 12.7%의 자연교잡율을 나타내었다. 화분친으로부터 15~90cm 거리 내에서는 가야벼 mS 및 남풍벼ms의 자연교잡율의 거리에 따른 차가 인정되지 않았다.

• 한국작물학회지
• /
• 제38권5호
• /
• pp.413-417
• /
• 1993

우리나라 japonica 벼의 일대잡종을 다양하게 육성할 목적으로 세포질 유전자적 웅성불임인 BTCMS에 대한 임성회복력이 있는 계통 AR-3을 교잡하여 그 F1을 모본으로 하고 조ㆍ중ㆍ만생의 한국 japonica형 벼 4품종씩을 교본 반복친으로 5회 backcross하여 우리나라 벼 품종 배경의 우엉불임 계통과 임성회복계통을 육성하고 이들의 주요 작물학적 특성을 각각의 반복친과 비교한 결과를 요약하면 다음과 같다. 1. 우리나라 japonica형 품종의 배경을 갖는 세포질 유전자적 웅성불임계통을 조ㆍ중ㆍ만생군으로 각각 4개씩 도합 12계통 육성하였으며, 동일한 품종의 배경을 갖는 임성회복계통친도 조ㆍ중ㆍ만생군으로 각각 4개씩 12계통 육성하였다. 2. 육성된 웅성불임계통은 조중군에서 소백벼 A, 오대벼,A, 관악벼A, 대성벼A, 중생군에서 화진벼A, 팔달A, 수원 224A, 이리 386A, 만생군에서 낙동벼A, 팔굉A, 화청벼A 및 밀양 97A 등으로 명명하였다. 3. 육성된 임성회복계통은 조생군에서 소백벼R, 오대벼R, 팔달R, 수원 224R, 이리 386R, 만생군에서 낙동벼R, 팔굉R, 화청벼R 및 밀양 97R 등으로 명명하였다. 4. 육성된 웅성불임계통들은 반복친(불임유지친)에 비하여 간장이 단축되는 경향이었으나 출수기, 수장 및 수량구성요소 등은 대체로 비슷하였다. 5. 육성된 임성회복계통들은 반복친과 비교하면 대체로 간장, 수장, 수량구성요소 및 수량의 차이가 없었다.