• Title/Summary/Keyword: crop mapping

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Development of RAPD Marker Related to Brown Planthopper Resistance Gene Derived from Rice Cultivar, Cheongcheongbyeo (청청벼에서 유래한 벼멸구 저항성관련 RAPD Marker의 개발)

  • Seo Ji-Hun;Kim Kyung-Min;Kim Suk-Man;Sohn Jea-Keun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.6
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    • pp.453-456
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    • 2005
  • This study was carried out to select DNA markers closely linked to brown planthopper (BPH) resistance gene originated from a rice cultivar 'Cheongcheong­byeo'. For the mapping of resistant gene to BPH, a doubled-haploid (DH) population was developed by anther culture of $F_1$ plants from a cross 'Cheongcheong­byeo/Nagdongbyeo'. In BPH bioassay and marker screen­ing for the DH population, the segregation of resistant and susceptible plants to BPH fitted to a 1:1 ratio. A total of 310 RAPDs of 520 markers showed polymorphism in parental survey using 'Cheongcheongbyeo' and 'Nag­dongbyeo'. In the analysis of relationship between BPH resistance and marker pattern for 40 DH lines, the OPE16 produced a specific dominant fragment, 700 bp, which was closely linked with BPH resistance gene of 'Cheong­cheongbyeo'. Based on the linkage analysis using 7 markers, BPH resistance of 'Cheongcheongbyeo' was mapped on chromosome 12, which was closely linked with $OPE16_{700}$ at a distance of 4.6 cM.

Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo1', a Japonica Rice Mutant Line (남일벼 돌연변이 후대 계통 'Namil(SA)-flo1'의 분질배유 특성에 대한 유전분석)

  • Mo, Young-Jun;Jeung, Ji-Ung;Kang, Kyung-Ho;Lee, Jeom-Sig;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.3
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    • pp.283-291
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    • 2013
  • Rice varieties with suitable flour-making quality are required to promote rice processed-food industry and boost rice consumption in Korea. 'Namil (SA)-flo1' is an advanced mutant line with floury endosperm which shows good flour-making quality under dry-milling process. Genetic analysis was carried out to localize the chromosomal region responsible for the floury endosperm of 'Namil (SA)- flo1'. By using 94 F2 progenies, which were derived from 'Namil (SA)-flo1' ${\times}$ 'Milyang 23', floury grains percentage was investigated as phenotypic data, and genotyping was conducted with 54 SSR markers. Association analysis showed that the target genetic region for floury endosperm is on middle-low region of chromosome 5. Through further association analysis with increased number of SSR markers on chromosome 5, we found that genotypic variation in RM164 explains 79.7% of the variation in floury grains percentage of F2:3 seeds. The floury endosperm locus was localized on 17.7-20.7 Mbp region of chromosome 5 and will be further analyzed for fine mapping and gene identification.

Mapping QTLs for Agronomic Traits Using an Introgressin Line Population from a Cross between Ilpumbyeo and Moroberekan in Rice (일품벼/모로베레칸 이입계통을 이용한 농업형질 관련 QTL 분석)

  • Ju, Hong-Guang;Kim, Dong-Min;Kang, Ju-Won;Kim, Myung-Ki;Kim, Yeon-Gyu;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.40 no.4
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    • pp.414-421
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    • 2008
  • We conducted a QTL analysis of agronomic traits using 117 $BC_3F_5$ and $BC_3F_6$ lines developed from a cross between Ilpumbyeo and Moroberekan. Genotypes of 117 $BC_3F_5$ lines were determined using 134 simple sequence repeat (SSR) markers. A total of 832 Moroberekan chromosome segments with 410 homozygous and 422 heterozygous, respectively, were detected, and the genetic distance of introgression segments ranged from 0.5 cm to 112.1 cm. A linkage map constructed using 134 SSR markers was employed to characterize quantitative trait loci (QTL). The 117 $BC_3F_5$ and $BC_3F_6$ lines were evaluated for seven agronomic traits at two locations in 2006 and 2007 and at one location in 2007. A total of 26 QTLs were identified for seven traits including days to heading, and the phenotypic variance explained by each QTL ranged from 9.2% to 24.2%. Moroberekan alleles contributed positive effects in the Ilpumbyeo background at eleven QTL loci including panicle length and spikelets per panicle. Five QTLs, two for days to heading and one each for culm length, panicle length and spikelets per panicle were consistently detected in every occasions indicating that these QTLs are stable. Among them, two QTLs, spp6 for spikelets per panicle and pl6 for paniclel length were localized in the similar region. Increase in spikelets per panicle at this locus might be due to the increase in panicle length, because both traits were associated with increase in spikelets per panicle and panicle length due to the presence of the Moroberekan allele. These Moroberekan QTLs might be useful in breeding programs to develop high-yielding cultivars.

Mapping landuse change and major food crops production in Nepal: Applications for forest resource management

  • Panta, Menaka;Kim, Kye-Hyun;Neupane, Hari Sharma;Joshi, Chudamani
    • Proceedings of the KSRS Conference
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    • 2008.10a
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    • pp.390-393
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    • 2008
  • We analyzed the landuse change, quantify the area covered by majors food crops (Paddy, Wheat, Barely, Maize, Millet and Potato) and their productivity trends in Terai, Nepal from 1987 to 2006. We used series of area covered by each single crop and production data published by Government of Nepal, Central Bureau of Statistics and Ministry of Agriculture and Cooperatives. Our results indicated that the agriculture land has increased by about 47% while forest has decreased by 32% between 1964 and 2001 in Terai. Whilst the total cropped area has increased by 19% between 1987 and 2006. The highest incremental change has observed in Potato by 234% followed by Wheat 31%, Maize 20% and Paddy 12% and so on. However, data revealed with very low crops productivity and it showed less than half of its potential except in Potato. The average yield of food crops /hectare /year during last 20 year has found only 3.094 metric tons. Only Potato has gained high average yield by 10.34 metric tons. While others crops yielded entirely low. 3 periods moving average depicted that the productivity trend of Barely and Millet has stagnant while others crops showed slightly up and down and increasing steadily over time. Further study is needed to comprehend the linkage of food productivity in the present food supply to demand and food security system in Terai, Nepal.

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A Study on Analysis of Natural Disaster Using Remote Sensing Data (원격탐사 자료를 이용한 자연재해분석에 관한 연구)

  • Park, Byung-Uk;Kim, Chul
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.15 no.2
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    • pp.237-244
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    • 1997
  • The goal of this research is to evaluate methodology that uses satellite data for the analysis of flood and drought damaged area. Land cover classification were performed using satellite data that were acquired at disaster periods and comparatively normal times. Damaged area was extracted by use of overlay analysis in land cover change and compared with the field survey results. The results show analysis of flood damaged area could be carried out with single scene acquired at adequate day, and are corresponded with field survey data very well. And also, some areas that had been missed in field survey were found. The suggested method proved to be more accurate and effective way for mapping inundated areas of floodplains than field survey that would be held a few month later. The results on the analysis of drought damaged area show that drained water could be detected just only in small area, and crop damaged area could not be verified in objective validity. Drought analysis by remote sensing was proved not to be adequate for practical use in this study.

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Mapping the Spatial Distribution of IRG Growth Based on UAV

  • Na, Sang-Il;Park, Chan-Won;Kim, Young-Jin;Lee, Kyung-Do
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.5
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    • pp.495-502
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    • 2016
  • Italian Ryegrass (IRG), which is known as high yielding and the highest quality winter annual forage crop, is grown in mid-south area in Korea. The objective of this study was to evaluate the use of unmanned aerial vehicle (UAV) for the monitoring IRG growth. Unmanned aerial vehicle imagery obtained from middle March to late May in Nonsan, Chungcheongnam-do. Unmanned aerial vehicle imagery corrected geometrically and atmospherically to calculate normalized difference vegetation index (NDVI). We analyzed the relationships between $NDVI_{UAV}$ of IRG and biophysical measurements such as plant height, fresh weight, and dry weight over an entire IRG growth period. The similar trend between $NDVI_{UAV}$ and growth parameters was shown. Correlation analysis between $NDVI_{UAV}$ and IRG growth parameters revealed that $NDVI_{UAV}$ was highly correlated with fresh weight (r=0.988), plant height (r=0.925), and dry weight (r=0.853). According to the relationship among growth parameters and $NDVI_{UAV}$, the temporal variation of $NDVI_{UAV}$ was significant to interpret IRG growth. Four different regression models, such as (1) Linear regression function, (2) Linear regression through the origin, (3) Power function, and (4) Logistic function were developed to evaluate the relationship between temporal $NDVI_{UAV}$ and measured IRG growth parameters. The power function provided higher accurate results to predict growth parameters than linear or logistic functions using coefficient of determination. The spatial distribution map of IRG growth was in strong agreement with the field measurements in terms of geographical variation and relative numerical values when $NDVI_{UAV}$ was applied to power function. From these results, $NDVI_{UAV}$ can be used as a new tool for monitoring IRG growth.

QTL Mapping of Agronomic Traits in an Advanced Backcross Population from a Cross between Oryza sativa L. cv. Milyang 23 and O. glaberrima

  • Kang, Ju-Won;Suh, Jung-Pil;Kim, Dong-Min;Oh, Chang-Sik;Oh, Ji-Min;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.40 no.3
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    • pp.243-249
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    • 2008
  • In the previous study, 141 $BC_3F_2$ lines from a cross between the Oryza sativa cv. Milyang 23 and O. glaberrima were used to identify favorable wild QTL alleles for yield component traits. In this study, we carried out QTL analysis of four grain morphology as well as four yield component traits using 141 $BC_3F_5$ lines from the same cross and compared QTLs detected in two different generations. The mean number of O. glaberrima segments in the 141 $BC_3F_5$ lines ranged from 1 to 13 with 2.69 and 5.71 of the average means of homozygous and heterozygous segments, respectively. There was a three-fold difference in the number of QTLs detected for four traits commonly evaluated in two generations (seven QTLs in the $BC_3F_5$ vs 21 in the $BC_3F_2$ population). The percentages of the phenotypic variance explained by QTLs in the BC3F5 population were similar to or less than those in the $BC_3F_2$ population. This is probably due to the difference in the genetic composition of two populations and the environmental effects. The locations of the QTLs commonly detected in both generations were in good agreement except for one QTL for spikelets per panicle. The yield QTL, yd3 was colocalized with the spikelets per panicle, spp3. Yield increase at this locus is due to the increase in spikelets per panicle, because both traits were associated with increase in spikelets per panicle and yield due to the presence of an O. glaberrima allele. Clusters of QTLs for grain morphology traits were observed in two chromosome regions. One cluster harboring five QTLs near SSR markers RM106 and RM263 was detected on chromosome 2. This population would serve as a foundation for development of the introgression line population from a cross between Milyang 23 and O. glaberrima.

Development and Characterization of Novel Rapeseed (Brassica napus L.) Mutant Lines through Mutation Breeding

  • Baul Yang;Sang Hoon Kim;Joon-Woo Ahn;Chang-Hyu Bae;Jaihyunk Ryu
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.23-23
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    • 2022
  • Rapeseed (Brassica napus L.) is one of the most valuable oilseed crop in the world. It is widely used in various industries, such as food, animal feed, energy and chemical industries. In order to improve the industrial requirements for rapeseed, useful agronomic characteristics (higher yields and disease resistance etc.) and modified oil traits (fatty acid composition and fat content) are important in rapeseed. However, Korea has limiting genetic resources of novel traits in rapeseed. In this research, novel rapeseed mutant genotypes by mutation breeding was developed. The mutant lines were generated by the treatment of the seeds of the original cultivar 'Tamra' with 700 Gy of gamma-ray (60Co). Mutants showing varied in flowering time, crude fat content, seed yield and fatty acid content that exhibited stable inheritance of the mutated characteristics from M5 to M7 generations were selected. We investigated genetic variation using SNPs identified from GBS analysis in rapeseed mutant lines derived from the gamma-ray, and interactions between the major agronomic and the oil traits. Significantly associated SNP loci were explored along with candidate genes using SNPs obtained by GBS analysis. As a results of association mapping, a total of 322 SNPs were significantly associated with agronomic traits (155 SNPs) and oil traits (167 SNPs). A total of 70 genes were annotated from agronomic characteristics SNPs; among them 7 genes significantly enriched in developmental process, and a total of 70 genes were annotated from crude fat content and fatty acid compositions SNPs; among them, 11genes were significantly enriched in biosynthetic process. These results could be used for the selection of rapeseed cultivar with enhanced qualities and potential economic benefits.

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Identification of Quantitative Trait Loci Associated with Anthracnose Resistance in Chili Pepper (Capsicum spp.) (고추 탄저병 저항성 관련 양적형질 유전자좌 분석)

  • Kim, Su;Kim, Ki-Taek;Kim, Dong-Hwi;Yang, Eun-Young;Cho, Myeong-Cheoul;Jamal, Arshad;Chae, Young;Pae, Do-Ham;Oh, Dae-Geun;Hwang, Ju-Kwang
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.1014-1024
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    • 2010
  • Pepper ($Capsicum$ spp.) anthracnose caused by $Colletotrichum$ $acutatum$ is a destructive disease susceptible to areas where chili peppers are grown. $Capsicum$ $baccatum$ var. $pendulum$ (Cbp) is resistant to anthracnose and has actively been used for interspecific hybridization for the introgression of resistance gene(s) into cultivated chili peppers. The goals of this study were to determine the inheritance of resistance to anthracnose within $Capsicum$ $baccatum$ and to map quantitative trait loci (QTLs) for the anthracnose resistance. A genetic mapping population consisting of 126 $F_2$ plants derived from a cross between $Capsicum$ $baccatum$ var. $pendulum$ (resistant) and $Capsicum$ $baccatum$ 'Golden-aji' (susceptible) was used for linkage mapping. The linkage map was constructed with 52 SSRs, 175 AFLPs, and 100 SRAPs covering 1,896cM, with an average interval marker distance of 4.0cM. Based on this map, the number, location, and effect of QTLs for anthracnose resistance were studied using plants inoculated in the laboratory and field. A total of 19 quantitative trait loci (2 major QTLs and 16 minor QTLs) were detected. Two QTLs ($An8.1$, $An9.1$) showed 16.4% phenotypic variations for anthracnose resistance after wounding inoculation. In addition, five minor QTL loci ($An7.3$, $An7.4$, $An4.1$, $An3.1$, $An3.2$) showed a total of 60.73% phenotypic variations of anthracnose resistance in the field test. Several significant QTLs were also detected and their reproducibility was confirmed under different inoculation conditions. These QTLs are now being confirmed with different breeding populations. Markers tightly linked to the QTLs that are reliable under different environmental conditions will help to determine the success of marker-assisted selection for anthracnose -resistant breeding programs in chili pepper.

Development of dry milling suitable rice cultivar to invigorate rice processing products

  • Jeung, Ji-Ung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.10-10
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    • 2017
  • Rice consumption has been continuously decreasing as the eating habits of Koreans have become westernized and diversified. The per capita annual rice consumption in Korea has dropped sharply from 136.4 kg in 1970 to 61.9 kg in 2016. The Korean government, therefore, has been trying to promote rice consumption by invigorating the processed food industry using rice flour. To facilitate the market for processed rice foods, it is essential to develop proper milling technology in terms of flour particle size and damaged starch content to produce high quality rice flour at competitive cost. Dry milling and wet milling are the two major processes used to produce rice flour. Although the dry milling process is relatively simple with a lower production cost, damaged starch content increases because of the high grain hardness of rice. In wet milling, the quality of rice flour is improved by reducing flour particle size as well as damaged starch content through soaking procedures. However, the production costs are high because of the additional expenses associated with the disposal of waste water, sterilization and drying of the wet flour. Recently developed technologies such as jet milling and cryogenic milling also require expensive investment and production. Therefore, developing new rice cultivars with dry milling adaptability as well as good processing properties is an important goal of rice breeding in Korea. 'Suweon 542' is a floury endosperm mutant line derived from sodium azide treatment on a high-yield, early maturing, and non-glutinous japonica rice cultivar, 'Namil'. Compared with the wild type, after dry milling process, the grain hardness of 'Suweon 542' was significantly lower because of its round and loosely packed starch granules. Also, the flour of 'Suweon 542' had significantly smaller particles and less damaged starch than 'Namil' and other rice cultivars and its particle size distribution was similar to a commercial wheat cultivar. Recently, through collaborations with nine universities and food companies, a total of 21 kinds of processed prototypes, using the dry milling flour of 'Suweon 542', were evaluated. In the production of major rice processing products, there was no significant quality difference between the flours prepared by wet milling and dry milling. Although the amount of water added to the dough was slightly increased, it was confirmed that the recipe applying the wet flour could be used without significant change. To efficiently transfer the floury endosperm characteristics of 'Suweon 542' to other commercial rice cultivars, it is essential to develop DNA marker tightly linked to the target gene. Association analysis using 70 genome-wide SSR markers and 94 F2 plants derived from 'Suweon 542'/'Milyang 23' showed that markers on chromosome 5 explained a large portion of the variation in floury grains percentage (FGP). Further analysis with an increased number of SSR markers revealed that the floury endosperm of 'Suweon 542' was directed by a major recessive locus, flo7(t), located in the 19.33-19.86 Mbp region of chromosome 5, with RM18639 explaining 92.2% of FGP variation in the F2 population. Through further physical mapping, a co-segregate and co-dominant DNA marker with the locus, flo7(t) was successfully developed, by which, thereby, breeding efficiency of rice cultivars having proper dry milling adaptability with high yield potential or useful functional materials would be improved. 'Suweon 542' maintained the early maturity of the wild type, Namil, which can be used in rice-wheat double cropping systems in Korea not only for improved arable land but also for sharing flour production facilities. In addition to the high susceptibility against major rice diseases, nevertheless, another possible drawback of 'Suweon 542' is the high rate of viviparous under prolonged rainfall during the harvesting season. To overcome susceptibility and vivipary of 'Suweon 542', the progeny lines, derived from the crosses 'Suweon 542' and 'Jopyeong', an early maturing rice cultivar with multiple resistance against rice blast, bacterial blight, and rice strip virus, and 'Heugjinju', a anthocyanin pigment containing black rice cultivar, were intensively evaluated. As the outputs, three dry milling suitable rice elite lines, 'Jeonju614', 'Jeonju615', and 'Jeonju616' were developed.

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