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Development of RAPD Marker Related to Brown Planthopper Resistance Gene Derived from Rice Cultivar, Cheongcheongbyeo  

Seo Ji-Hun (Department of Agronomy Graduate School, Kyungpook National University)
Kim Kyung-Min (Department of Environmental Horticulture, Sangju National University)
Kim Suk-Man (Department of Agronomy Graduate School, Kyungpook National University)
Sohn Jea-Keun (Department of Agronomy Graduate School, Kyungpook National University)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.50, no.6, 2005 , pp. 453-456 More about this Journal
Abstract
This study was carried out to select DNA markers closely linked to brown planthopper (BPH) resistance gene originated from a rice cultivar 'Cheongcheong­byeo'. For the mapping of resistant gene to BPH, a doubled-haploid (DH) population was developed by anther culture of $F_1$ plants from a cross 'Cheongcheong­byeo/Nagdongbyeo'. In BPH bioassay and marker screen­ing for the DH population, the segregation of resistant and susceptible plants to BPH fitted to a 1:1 ratio. A total of 310 RAPDs of 520 markers showed polymorphism in parental survey using 'Cheongcheongbyeo' and 'Nag­dongbyeo'. In the analysis of relationship between BPH resistance and marker pattern for 40 DH lines, the OPE16 produced a specific dominant fragment, 700 bp, which was closely linked with BPH resistance gene of 'Cheong­cheongbyeo'. Based on the linkage analysis using 7 markers, BPH resistance of 'Cheongcheongbyeo' was mapped on chromosome 12, which was closely linked with $OPE16_{700}$ at a distance of 4.6 cM.
Keywords
BPR; RAPD; MAS; rice;
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1 Yeo, U. S., D. Y. Kwak, S. J. Lim, W. G. Ha, J. H. Cho, and J. K. Sohn. 2002. Relationship between agronomic traits and resistance to brown planthopper in japonica RIL population. Korean J. Breed. 34(3) : 148-152
2 Hirabayashi, H. and O. Tsugufumi. 1995. RFLP mapping of Bph 1 (Brown Planthopper Resistnace Gene) in rice. Breeding Science 45 : 369-371
3 Huang, Z., G. He, L. Shu, X. Li, and Q. Zhang. 2001. Identification and mapping of two brown planthopper resistance genes in rice. Theor. Appl. Genet. 102 : 929-934   DOI   ScienceOn
4 Jeon, Y. H., S. N. Ahn, H. C. Choi, T. R. Hahn, and H. P. Moon. 1999. Identification of a RAPD marker linked to a brown planthopper resistance gene in rice. Euphytica 107 : 23-28   DOI   ScienceOn
5 Murata, K., M. Fujiware, C. Kaneda, S. Takumi, N. Mori, and C. Nakamura. 1998, RFLP mapping of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph 2 of indica rice intorogressed into a japonica breeding line Norin-PL4, Genes Genet. Syst. 73 : 359-364   DOI   ScienceOn
6 Kim, S. M., S. J. Yang, and J. K. Sohn. 2003, Development ofa DNA Marker for selection of resistanct plants to brown planthopper (BPH) in rice. Korean J. Breed 35(5) : 277-282
7 Chung, G. S. and J. K. Sohn. 1995. Anther culture technology in rice. In : Kannaiyan S., ed, Rice Management Biotechnology. Associated Pub. Co. New Delhi, pp 1-9
8 Jena, K. K., I. C. Pasalu, Y. K. Rao, Y. Varalaxmi, K. Krishnaiah, G. S. Khush, and G. Kochert. 2002. Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Euphytica 129 : 81-88   DOI   ScienceOn
9 Sharma, P. N., A. Torii, S. Takumi, N. Mori, and C. Nakamura. 2004. Marker-assisted pyramiding of brown planthopper (Nilaparvata lugens Stal) resistance genes Bph1 and Bph2 on rice chromosome 12. Hereditas. 140 : 61-69   DOI   ScienceOn
10 Ha W. H., K. M. Kim, and J. K. Sohn. 1999. Detection of resistnce gene to brown plnathopper(Nilaparvata lugens Stal.) using RFLP and Isozyme marker in rice. Korean J. Breed 32(4) : 319-322