• 제목/요약/키워드: crop disease

검색결과 1,153건 처리시간 0.04초

유묘검정법을 이용한 궤양병 저항성 토마토품종 선발 (Screening of Tomato Cultivars Resistant to Bacterial Canker by Seedling Test)

  • 한유경;한경숙;이성찬;김형환;김수;김동휘
    • 식물병연구
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    • 제16권3호
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    • pp.290-293
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    • 2010
  • 국내에서 Clavibacter michiganensis subsp. michiganensis에 의한 궤양병은 토마토 농가에 심각한 피해를 주고 있다. 이에 병 발생의 감소와 방제를 위해서는 저항성 품종이 최선의 해결책으로 제시되고 있다. 따라서 국내에서 시판되고 있는 완숙토마토 39품종, 방울토마토 20품종, 대목토마토 13품종에 대한 궤양병 저항성 품종을 선별하기 위해 저항성 검정을 실시하였다. 시판되는 72품종 중 선명, 스위트, 다다끼, 아기꼬, 매치, 마그네트, 프렌즈, 그린파워, 품종이 궤양병에 대해 저항성을 나타내었다.

Pi5 and Pii Paired NLRs Are Functionally Exchangeable and Confer Similar Disease Resistance Specificity

  • Vo, Kieu Thi Xuan;Lee, Sang-Kyu;Halane, Morgan K.;Song, Min-Young;Hoang, Trung Viet;Kim, Chi-Yeol;Park, Sook-Young;Jeon, Junhyun;Kim, Sun Tae;Sohn, Kee Hoon;Jeon, Jong-Seong
    • Molecules and Cells
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    • 제42권9호
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    • pp.637-645
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    • 2019
  • Effector-triggered immunity (ETI) is an effective layer of plant defense initiated upon recognition of avirulence (Avr) effectors from pathogens by cognate plant disease resistance (R) proteins. In rice, a large number of R genes have been characterized from various cultivars and have greatly contributed to breeding programs to improve resistance against the rice blast pathogen Magnaporthe oryzae. The extreme diversity of R gene repertoires is thought to be a result of co-evolutionary history between rice and its pathogens including M. oryzae. Here we show that Pii is an allele of Pi5 by DNA sequence characterization and complementation analysis. Pii-1 and Pii-2 cDNAs were cloned by reverse transcription polymerase chain reaction from the Pii-carrying cultivar Fujisaka5. The complementation test in susceptible rice cultivar Dongjin demonstrated that the rice blast resistance mediated by Pii, similar to Pi5, requires the presence of two nucleotide-binding leucine-rich repeat genes, Pii-1 and Pii-2. Consistent with our hypothesis that Pi5 and Pii are functionally indistinguishable, the replacement of Pii-1 by Pi5-1 and Pii-2 by Pi5-2, respectively, does not change the level of disease resistance to M. oryzae carrying AVR-Pii. Surprisingly, Exo70F3, required for Pii-mediated resistance, is dispensable for Pi5-mediated resistance. Based on our results, despite similarities observed between Pi5 and Pii, we hypothesize that Pi5 and Pii pairs require partially distinct mechanisms to function.

Descriptor 조합 및 동일 병명 이미지 수량 역비율 가중치를 적용한 유사도 기반 작물 질병 검색 기술 설계 및 구현 (Design and Implementation of a Similarity based Plant Disease Image Retrieval using Combined Descriptors and Inverse Proportion of Image Volumes)

  • 임혜진;정다운;유성준;구영현;박종한
    • 한국차세대컴퓨팅학회논문지
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    • 제14권6호
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    • pp.30-43
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    • 2018
  • 영상의 특징인 색상, 모양, 질감 등을 이용해 영상을 검색하는 연구들은 많이 진행되어 왔다. 또한 작물의 질병 영상과 관련된 연구들도 진행되고 있다. 농업 현장에서 재배되는 작물에 발생한 질병을 확인하는데 도움이 되기 위해 본 논문에서는 시설원예 작물의 질병 영상을 이용한 유사도 기반 작물 질병 검색 시스템을 제안한다. 제안하는 시스템은 단일 Descriptor를 사용하지 않고, 조합 Descriptor를 통해 기존 대비 영상의 유사도 검색 성능을 높였고 유사도 검색 결과를 가독성 높게 사용자에게 제공하기 위해 가중치 기반 산출방법을 적용했다. 본 논문에서는 총 13개의 개별 Descriptor를 이용해 조합을 진행했다. 조합 Descriptor를 이용해 6개 작물의 질병에 대해 유사도 검색을 진행했고 작물별로 평균 accuracy가 높은 조합 Descriptor를 선정해 유사도 검색에 사용했다. 검색된 결과는 병명의 비율을 기반으로 한 산출방법과 가중치를 기반으로 한 산출방법을 사용해 백분율로 나타냈다. 병명의 비율을 기반으로 한 산출방법은 질의 영상과 유사도 검색에 사용되는 영상의 수가 많은 병명이 1순위로 출력되는 문제점이 있다. 이를 해결하기 위해 가중치를 기반으로 한 산출방법을 사용했다. 작물의 병명별 테스트 영상을 두 가지 산출방법에 적용해 검색 성능을 측정했다. 작물의 질병별로 두 가지 산출방법에 대해 검색 성능 값의 평균을 비교한 결과 고추, 사과 작물에서는 병명의 비율을 기반으로 한 산출방법의 성능이 가중치를 기반으로 한 산출방법의 성능보다 평균 약 11.89%의 높은 성능 결과를 보였다. 국화, 딸기, 배, 포도 작물에서는 가중치를 기반으로 한 산출방법이 병명의 비율을 기반으로 한 산출방법의 성능보다 평균 약 20.34%의 높은 성능 결과를 보였다. 또한 본 논문에서 제안하는 시스템의 UI/UX는 실제 사용자의 피드백을 통해 편리하게 구성했다. 시스템의 화면마다 상단에 제목과 설명을 출력했고 사용자가 질병의 정보를 보기 편리하게 화면을 구성했다. 검색된 질병의 정보는 위에서 제안한 산출방법을 토대로 유사한 질병의 영상과 병명을 출력한다. 시스템의 환경은 PC 환경 기반의 웹 브라우저와 모바일 디바이스 환경 기반의 웹 브라우저를 통해 사용할 수 있도록 구현했다.

작물에 함유된 Phytoestrogen의 특성과 생리활성 (Biological Activities of Phytoestrogens in Plant and Foodstuff)

  • 김성란;최선영;안지윤;하태열
    • 한국작물학회지
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    • 제48권
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    • pp.31-40
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    • 2003
  • Phytoestrogens are oestrogenic compounds found in plants and consist of isoflavones, lignans, and coumestans. The structural similarity of phytoestrogens to endogenous oestrogens has promoted the hypothesis that phytoestrogens exert hormonal or anti-hormonal effects relevant to the risk of hormone-dependent disease and/or their suitability as a dietary alternative to hormone replacement therapy. Epidemiological studies suggest that food stuffs containing phytoestrogens may have a beneficial role in protecting against a number of chronic disease and conditions. It is thought that these estrogen-like compounds may protect against chronic diseases, such as hormone-dependent cancers, cardiovascular disease and osteoporosis. Furthermore, phytoestrogens are used as a natural alternative to hormone replacement therapy and to reduce menopausal symptoms. Phytoestrogens are considered good candidates for use in natural therapies and as chemopreventive agents in adults. However safe and efficacious levels have yet to be established.

Resistance of Fusarium fujikuroi Isolates to Hydrogen Peroxide and Its Application for Fungal Isolation

  • Youn, Kihoon;Choi, Hyo-Won;Shin, Dong Bum;Jung, Boknam;Lee, Jungkwan
    • 식물병연구
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    • 제21권3호
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    • pp.227-230
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    • 2015
  • The ascomycete fungus Fusarium fujikuroi causes bakanae disease in rice and this disease has been reemerging in Korea. Other fungal species including F. graminearum and Magnaporthe oryzae are often associated with F. fujikuroi, hampering pure isolation of F. fujikuroi from rice. In this study, we modified a selective medium for F. fujikuroi as supplementing both pentachloronitrobenzene and hydrogen peroxide into minimal medium. This medium efficiently suppressed the vegetative growth of F. graminearum and M. oryzae, but did not significantly reduce F. fujikuroi growth, providing an efficient tool for isolating F. fujikuroi.

Impacts of Different Pentachloronitrobenzene (quintozene) Use Patterns on Severity of Damping-off of Ginseng (Panu quinquefolitrs)

  • Reeleder, R.D;Capell, B
    • Journal of Ginseng Research
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    • 제26권1호
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    • pp.10-16
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    • 2002
  • In replicated field trials, the efficacy of pentachloronitrobenzene (PCNB; quintozene) for control of damping-off of ginseng seedlings was found to be affected by timing of application and formulation. Application at the time of seeding and prior to placement of straw mulch was found to provide the moat consistent level of disease control. However, decline in plant stand during the four-year production cycle resulted in most treatments providing similar levels of plant populations at harvest. Soil residues of pentachloronitrobenzene were generally highest (1 $\mu\textrm{g}$ PCNB/g soil) in those treatments that exhibited the highest levels of disease control in the seedling year. Straw contained high levels of quintozene after application. Beet seed assays with artificially-infested soils indicated that current use rates provide an amount of product suitable for high levels of disease control.

감자유전자 StACRE의 분리 및 풋마름병 저항성 기능 검정 (Isolation of Potato StACRE Gene and Its Function in Resistance against Bacterial Wilt Disease)

  • 박상렬;차은미;김태훈;한세연;황덕주;안일평;조광수;배신철
    • 생명과학회지
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    • 제22권2호
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    • pp.177-183
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    • 2012
  • Ralstonia solanacearum (Rs)에 의해 유발되는 풋마름병은 감자 재배 시 발병하는 주요 병 중의 하나이다. 감자에서 풋마름병 저항성관련 유전자를 찾기 위해 기존에 기능이 알려진 다른 가지과 작물의 기능 유사 유전체를 이용하여 StACRE (HM749652) 유전자를 분리하고 염기서열을 분석하였다. 분리한 StACRE의 발현양상을 분석하기 위해 병 저항성 유도 신호전달 물질인 SA와 풋마름병원균 Rs (KACC10722)를 처리한 감자에서 RNA를 추출하여 RT-PCR을 실시한 결과 이 유전자는 SA 처리에 의해 3시간 후부터, Rs에 의해서는 12시간 후부터 발현이 현저하게 증가하였다. 따라서, 감자에서 이 유전자의 생물학적인 기능을 분석하기 위해 Gateway System을 이용하여 과발현용 vector를 만든 후 과발현 형질전환 감자를 제작하고 풋마름병균인 Rs를 접종하여 병 저항성 기능을 검정 한 결과 대조구인 수미 감자에 비해 병 저항성이 증대하였다.

녹비작물 토양환원과 태양열 소독에 의한 3년생 인삼의 뿌리썩음병 억제효과 (Effect of Green Manure Incorporation and Solarization on Root Rot Disease of 3-year-old Ginseng in Soil of Continuous Cropping Ginseng)

  • 서문원;이성우;이승호;장인복;허혜지
    • 한국약용작물학회지
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    • 제27권4호
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    • pp.284-291
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    • 2019
  • Background: Ginseng root rot disease, caused by Cylindrocarpon destructans and Fusarium solani is a major cause of replant failure in continuous cropping ginseng. Methods and Results: To control replant injury in soil infected with C. destructans and F. solani, biosolarization was performed by covering the plot with transparent polyethylene film after adding green manure of maize and sunflower for the summer season. Per 10 a, fresh and dry weight of maize was 10.1 and 2.5 tons, respectively, and that of sunflower was 8.1 tons and 1.2 tons, respectively. Mean maximum temperature at 20 cm depth was $33.2^{\circ}C$, $41.5^{\circ}C$ and $41.8^{\circ}C$ in the control, maize-incorporated and sunflower-incorporated plots, respectively. The elapsed time over $40^{\circ}C$ was 36.4 h in the maize-incorporated plot and 77.3 h in the sunflower-incorporated plot. Biosolarization increased $NO_3$ content in soil, while content of organic matter, Ca, and Mg was decreased. Electrical conductivity, $NO_3$ and $P_2O_5$ in soil significantly increased after two years of biosolarization. The number of spores of C. destructans in soil was significantly decreased by biosolarization, and sunflower treatment was more effective than maize treatment in decreasing the number of spores. Root yield of 3-year-old ginseng was significantly increased by biosolarization, however, there was no significant difference between maize and sunflower treatments. Rate of root rot in 3-year-old ginseng decreased to 16.5% with the incorporation maize and 5.0% with the incorporation of sunflower, while that in control 25.6%. Conclusions: Biosolarization was effective in inhibiting ginseng root rot by decreasing the density of root rot disease and improving soil chemical properties.

Comparative Pathogenicity of Fusarium graminearum Isolates from Wheat Kernels in Korea

  • Shin, Sanghyun;Son, Jae-Han;Park, Jong-Chul;Kim, Kyeong-Hoon;Yoon, Young-mi;Cheong, Young-Keun;Kim, Kyong-Ho;Hyun, Jong-Nae;Park, Chul Soo;Dill-Macky, Ruth;Kang, Chon-Sik
    • The Plant Pathology Journal
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    • 제34권5호
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    • pp.347-355
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    • 2018
  • Fusarium head blight (FHB) caused by Fusarium species is a major disease of wheat and barley around the world. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins including; nivalenol (NIV), deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON). The objectives of this study were to identify strains of F. graminearum isolated in Korea from 2012-harvested wheat grain and to test the pathogenicity of these NIV- and DON-producing isolates. Three hundred and four samples of wheat grain, harvested in 2012 in Chungnam, Chungbuk, Gyeongnam, Jeonbuk, Jeonnam, and Gangwon provinces were collected. We recovered 44 isolates from the 304 samples, based on the PCR amplification of internal transcribed spacer (ITS) rRNA region and sequencing. Our findings indicate that F. asiaticum was the predominant (95% of all isolates) species in Korea. We recovered both F. asiaticum and F. graminearum from samples collected in Chungnam province. Of the 44 isolates recovered, 36 isolates had a NIV genotype while 8 isolates belonged to the DON genotype (3-ADON and 15-ADON). In order to characterize the pathogenicity of the strains collected, disease severity was assessed visually on various greenhouse-grown wheat cultivars inoculated using both NIV- and DON-producing isolates. Our results suggest that Korean F. graminearum isolates from wheat belong to F. asiaticum producing NIV, and both F. graminearum and F. asiaticum are not significantly different on virulence in wheat cultivars.