• Journal of the Society of Cosmetic Scientists of Korea
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• v.30 no.1
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• pp.85-91
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• 2004

In this study, we evaluated the crow's feet of 152 Korean women by using visual assessment established by our research group and mechanical assessment, Skin Visiometer SV 600. The volunteers were divided into 3 groups according to age; the group I of 30's was 43, the group II of 40's was 75, the group III of 50's was 34. The wrinkle score by visual assessment showed tendency to increase according to age and was statistically significant different among those three age groups (P 〈 0.05). But there was no significant difference between the left and the right of crow's feet. The 5 wrinkle parameters (RI, R2, R3, R4, R5) of mechanical assessment varied as age and the Rl, R4, and R5 showed statistically significant difference among three age groups (P 〈 0.05). However there was no significant difference between the left and the right of crow's feet. The volunteers were divided into 3 groups according to wrinkle score; (0-3 grade for group A, 4-6 grade for group B, 7-9 grade for group C). Only R4 and R5 showed significant difference between group A and group C (P 〈 0.05). It was suggested that visual scoring criteria applied in this study was related to R4 and R5 and was useful in investigating in fine wrinkles.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.2
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• pp.191-200
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• 2018

In this study, we conducted research to find anti-wrinkle skin care ingredients from natural sources via the measurement of procollagen type 1 levels in the medium of normal human dermal fibroblast (NHDF) and found that a Nelumbo nucifera leave extract (NLE) was as the best effective ingredient. By the high performance liquid chromatography analysis, we confirmed isoquercitrin was one of the main compounds of NLE. Moreover, it also increased the procollagen type 1 production without cytotoxicity of NHDF. NLE and isoquercitrin exerted free radical scavenging activity. Especially, isoquercitrin exhibited strong intracellular antioxidant capacity in human skin-derived cells, HaCaT and NHDF. Finally, we performed clinical test for the inhibitory effect of NLE on skin wrinkle formation. A randomized study was conducted in 22 healthy female volunteers, aged between 30 and 65 yrs, with moderate to moderately severe facial wrinkles. Volunteer applied a 1.7% NLE cream (isoquercitrin 51 ppm) and a control cream at each facial side (left/right) twice daily for 8 weeks. The 1.7% NLE cream improved skin roughness through reducing the wrinkle of the craw's feet significantly without any skin side effect. Our results demonstrate that NLE and isoquercitrin can increase the collagen production and exert antioxidant activity. Therefore, we expect that the new non-toxic herbal extract, isoquercitrin-containing NLE will be interesting natural ingredient of cosmetics with anti-wrinkle efficacy.