• Title/Summary/Keyword: coupled model

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Biochemical Characterization of a GDSL-Motif Esterase from Bacillus sp. K91 with a New Putative Catalytic Mechanism

  • Ding, Junmei;Yu, Tingting;Liang, Lianming;Xie, Zhenrong;Yang, Yunjuan;Zhou, Junpei;Xu, Bo;Li, Junjun;Huang, Zunxi
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1551-1558
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    • 2014
  • The esterase gene Est8 from the thermophilic bacterium Bacillus sp. K91 was cloned and expressed in Escherichia coli. The monomeric enzyme exhibited a theoretical molecular mass of 24.5 kDa and an optimal activity around $50^{\circ}C$ at pH 9.0. A model of Est8 was constructed using a hypothetical YxiM precursor structure (2O14_A) from Bacillus subtilis as template. The structure showed an ${\alpha}/{\beta}$-hydrolase fold and indicated the presence of a typical catalytic triad consisting of Ser-11, Asp-182, and His-185, which were investigated by site-directed replacements coupled with kinetic characterization. Asp-182 and His-185 residues were more critical than the Ser-11 residue in the catalytic activity of Est8. A comparison of the amino acid sequence showed that Est8 could be grouped into the GDSL family and further classified as an SGNH hydrolase. Est8 is a new member of the SGNH hydrolase subfamily and may employ a different catalytic mechanism.

MT response on the two dimensional anisotropic structure (2차원 이방성 구조의 MT 반응)

  • Lee, Chun Gi;Gwon, Byeong Du
    • Journal of the Korean Geophysical Society
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    • v.2 no.2
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    • pp.123-134
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    • 1999
  • Magnetotelluric responses may be affected by strong anisotropy of the high-conductivity layers (HCL) in the upper mantle or lower crust. We have studied two-dimensional anisotropy MT modelling to examine the effect of high anisotropic media. Electrical properties of a homogeneous anisotropic body are defined by a symmetric conductivity tensor and the problem is described by coupled diffusion equation in the frequency domain. In two-dimensional anisotropic environments, diagonal elements of the impedance tensor have higher values than those in isotropic environments. In some cases, TM mode phases reach more than 90°and apparent resistivities decrease for some frequency range because of telluric distortion. GB decomposition may be used to recover regional responses, but can be affected by the regional anisotropic effect. Considering these results, BC87 dataset was interpreted with a modified anisotropic model.

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A Design And Implementation Of Simple Neural Networks System In Turbo Pascal (단순신경회로망의 설계 및 구현)

  • 우원택
    • Proceedings of the Korea Association of Information Systems Conference
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    • 2000.11a
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    • pp.1.2-24
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    • 2000
  • The field of neural networks has been a recent surge in activity as a result of progress in developments of efficient training algorithms. For this reason, and coupled with the widespread availability of powerful personal computer hardware for running simulations of networks, there is increasing focus on the potential benefits this field can offer. The neural network may be viewed as an advanced pattern recognition technique and can be applied in many areas such as financial time series forecasting, medical diagnostic expert system and etc.. The intention of this study is to build and implement one simple artificial neural networks hereinafter called ANN. For this purpose, some literature survey was undertaken to understand the structures and algorithms of ANN theoretically. Based on the review of theories about ANN, the system adopted 3-layer back propagation algorithms as its learning algorithm to simulate one case of medical diagnostic model. The adopted ANN algorithm was performed in PC by using turbo PASCAL and many input parameters such as the numbers of layers, the numbers of nodes, the number of cycles for learning, learning rate and momentum term. The system output more or less successful results which nearly agree with goals we assumed. However, the system has some limitations such as the simplicity of the programming structure and the range of parameters it can dealing with. But, this study is useful for understanding general algorithms and applications of ANN system and can be expanded for further refinement for more complex ANN algorithms.

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Magnetic Exchange Interactions in a 2D Grid-like Copper(II) Polymer with Bridging End-on Cyanato and Pyrazine Ligands: A DFT Study

  • Kang, Dae-Bok
    • Bulletin of the Korean Chemical Society
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    • v.31 no.6
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    • pp.1704-1710
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    • 2010
  • The structure of a 2D grid-like copper(II) complex [Cu$(NCO)_2$(pyz)](pyz=pyrazine) (1) consists of 1D chains of Cu-pyz units connected by double end-on (EO) cyanato bridges. Each Cu(II) ion has a distorted octahedral coordination, completed by the four EO cyanato and two pyrazine ligands. Magnetic interactions through EO cyanato and pyrazine bridges in 1 are discussed on the basis of DFT broken-symmetry calculations at the B3LYP level. For model dicopper(II) complexes I (bridged by cyanato) and II (bridged by pyrazine), electronic structure calculations reproduce very well the experimental couplings for the S = 1/2 ferromagnetic and antiferromagnetic exchange-coupled 2D system: the calculated exchange parameters J are +1.25 $cm^{-1}$ and -3.07 $cm^{-1}$ for I and II, respectively. The $\sigma$ orbital interactions between the Cu $x^2-y^2$ magnetic orbitals and the nitrogen lone-pair orbitals of pyrazine are analyzed from the viewpoint of through-bond interaction. The energy splitting of 0.106 eV between two SOMOs indicates that the superexchange interaction should be antiferromagnetic in II. On the other hand, there are no bridging orbitals that efficiently connect the two copper(II) magnetic orbitals in I because the HOMOs of the basal-apical NCO bridge do not play a role in the formation of overlap interaction pathway. The energy separation in the pair of SOMOs of I is calculated to be very small (0.054 eV). This result is consistent with the occurrence of weakly ferromagnetic properties in I.

A Numerical Study of the Residual Hydrogen Concentration in the Weld Metal (용접금속 잔류수소농도의 수치해석 연구)

  • Yoo, Jinsun;Ha, Yunsok;S.R., Rajesh
    • Journal of Welding and Joining
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    • v.34 no.6
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    • pp.42-46
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    • 2016
  • Hydrogen assisted cracking (HAC) is one of the most complicated problem in welding. Huge amount of studies have been done for decades. Based on them, various standards have been established to avoid HAC. But it is still a chronic problem in industrial field. It is well known that the main causes of the hydrogen crack are residual stress, crack susceptible micro structures and a certain critical level of hydrogen concentration. Even though the exact generating mechanism is unclear till today, it has been reported that the hydrogen level in the weld metal should be managed less than a certain amount to prevent it. Matsuda studied that the residual hydrogen level in the weld metal can be varied even if the initial hydrogen content is same. It is also insisted in this report that the residual hydrogen concentration is in stronger correlation with hydrogen crack than the initial hydrogen content. But, in practical point of view, the residual hydrogen is still hard to consider because measuring hydrogen level is time and cost consuming process. In this regard, numerical analysis is the only solution for considering the residual hydrogen content. Meanwhile, Takahashi showed the possibility of predicting the residual hydrogen by a rigorous FE analysis. But, few commercial software suitable for solving the weld metal hydrogen has been reported yet. In this study, two dimensional thermal - hydrogen coupled analysis was developed by using the commercial FE software MARC. Since the governing equation of the hydrogen diffusion is similar to the heat transfer, it is shown that the heat transfer FE analysis in association with hydrogen diffusion property can be used for hydrogen diffusion analysis. A series of simulation was performed to verify the accuracy of the model. For BOP (Bead-On-Plate) and the multi-pass butt welding simulations, remaining hydrogen contents in the weld metal is well matched with measurements which are referred from Kim and Masamitsu.

A Review on the Photochemical Oxidant Modeling as Applied to Air Quality Studies in Complex Terrain

  • Lee Hwa-Woon;Kim Yoo-Keun;Won Gyeong-Mee;Park Jong-Kil
    • Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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    • v.1 no.1
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    • pp.19-33
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    • 1997
  • The high oxidants, which occur the daily maximum concentrations in the afternoon, are transported into the other region via long range transport mechanisms or trapped within the shallow mixing boundary layer and then removed physically (deposition, transport by mountain wind, etc.) and chemically (reaction with local sources). Therefore, modeling formation of photochemical oxidants requires a complex description of both chemical and meteorological processes. In this study, as a part of air quality studies, we reviewed various aspects of photochemical modeling on the basis of currently available literature. The result of the review shows that the model is based on a set of coupled continuity equations describing advection, diffusion, transport, deposition, chemistry, emission. Also photochemical oxidant models require a large amount of input data concerned with all aspects of the ozone life cycle. First, emission inventories of hydrocarbon and nitrogen oxides, with appropriate spatial and temporal resolution. Second, chemical and photochemical data allowing the quantitative description of the formation of ozone and other photochemically-generated secondary pollutants. Third, dry deposition mechanisms particularly for ozone, PAN and hydrogen peroxide to account for their removal by absorption on the ground, crops, natural vegetation, man-made and water surfaces. Finally, meteorological data describing the transport of primary pollutants away from their sources and of secondary pollutants towards the sensitive receptors where environmental damage may occur. In order to improve our present study, shortcomings and limitation of existing models are pointed out and verification process through observation is emphasized.

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The Application of Wind Profiler Data and Its Effects on Wind Distributions in Two Different Coastal Areas (연안지역 지형적 특성에 따른 윈드프로파일러 자료의 자료동화 효과 분석)

  • Jeong, Ju-Hee;Lo, So-Young;Song, Sang-Keun;Kim, Yoo-Keun
    • Journal of Environmental Science International
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    • v.19 no.6
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    • pp.689-701
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    • 2010
  • The effects of high-resolution wind profiler (HWP) data on the wind distributions were evaluated in two different coastal areas during the study period (23-26 August, 2007), indicating weak-gradient flows. The analysis was performed using the Weather Research and Forecasting (WRF) model coupled with a three-dimensional variational (3DVAR) data assimilation system. For the comparison purpose, two coastal regions were selected as: a southwestern coastal (SWC) region characterized by a complex shoreline and a eastern coastal (EC) region surrounding a simple coastline and high mountains. The influence of data assimilation using the HWP data on the wind distributions in the SWC region was moderately higher than that of the EC region. In comparison between the wind speed and direction in the two coastal areas, the application of the HWP data contributed to improvement of the wind direction distribution in the SWC region and the wind strength in the EC region, respectively. This study suggests that the application of the HWP data exerts a large impact on the change in wind distributions over the sea and thus can contribute to the solution to lack of satellite and buoy data with their observational uncertainty.

Reduction of Electromagnetic Field from Wireless Power Transfer Using a Series-Parallel Resonance Circuit Topology

  • Kim, Jong-Hoon;Kim, Hong-Seok;Kim, In-Myoung;Kim, Young-Il;Ahn, Seung-Young;Kim, Ji-Seong;Kim, Joung-Ho
    • Journal of electromagnetic engineering and science
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    • v.11 no.3
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    • pp.166-173
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    • 2011
  • In this paper, we implemented and analyzed a wireless power transfer (WPT) system with a CSPR topology. CSPR refers to constant current source, series resonance circuit topology of a transmitting coil, parallel resonance circuit topology of a receiving coil, and pure resistive loading. The transmitting coil is coupled by a magnetic field to the receiving coil without wire. Although the electromotive force (emf) is small (about 4.5V), the voltage on load resistor is 148V, because a parallel resonance scheme was adopted for the receiving coil. The implemented WPT system is designed to be able to transfer up to 1 kW power and can operate a LED TV. Before the implementation, the EMF reduction mechanism based on the use of ferrite and a metal shield box was confirmed by an EM simulation and we found that the EMF can be suppressed dramatically by using this shield. The operating frequency of the implemented WPT system is 30.7kHz and the air gap between two coils is 150mm. The power transferred to the load resistor is 147W and the real power transfer efficiency is 66.4 %.

The Effects of Entrepreneurship on Innovation Performance - Focusing on the Mediating Effect of Social Capital and Intellectual Capital - (기업가정신이 혁신성과에 미치는 영향에 관한 연구 - 사회적 자본과 지적자본의 매개효과를 중심으로 -)

  • Ahn, Chang-Heun;Kim, Sun Geun
    • Management & Information Systems Review
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    • v.36 no.2
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    • pp.261-276
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    • 2017
  • One of the main objectives of this paper is to identify the mediating effects of both social and intellectual capitals in the relations between entrepreneurship and innovation performance in Small and Medium Businesses. Based on the previous literatures on their relationships, we set our research model and test our hypotheses through a questionnaire survey. The main results of our analyses are as followings: first, entrepreneurship has a significant effect on innovation performance; second, social capital has a significant mediating effect between entrepreneurship and innovation performance; and finally, there exists a significant mediating effect between intellectual capital and entrepreneurship. One of the main policy implications of this study would be that the level of both social and intellectual capitals, coupled with entrepreneurship, should be reinforced in order to improve innovation performance.

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Application of support vector machine with firefly algorithm for investigation of the factors affecting the shear strength of angle shear connectors

  • Chahnasir, E. Sadeghipour;Zandi, Y.;Shariati, M.;Dehghani, E.;Toghroli, A.;Mohamad, E. Tonnizam;Shariati, A.;Safa, M.;Wakil, K.;Khorami, M.
    • Smart Structures and Systems
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    • v.22 no.4
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    • pp.413-424
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    • 2018
  • The factors affecting the shear strength of the angle shear connectors in the steel-concrete composite beams can play an important role to estimate the efficacy of a composite beam. Therefore, the current study has aimed to verify the output of shear capacity of angle shear connector according to the input provided by Support Vector Machine (SVM) coupled with Firefly Algorithm (FFA). SVM parameters have been optimized through the use of FFA, while genetic programming (GP) and artificial neural networks (ANN) have been applied to estimate and predict the SVM-FFA models' results. Following these results, GP and ANN have been applied to develop the prediction accuracy and generalization capability of SVM-FFA. Therefore, SVM-FFA could be performed as a novel model with predictive strategy in the shear capacity estimation of angle shear connectors. According to the results, the Firefly algorithm has produced a generalized performance and be learnt faster than the conventional learning algorithms.