• Applied Biological Chemistry
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• v.38 no.3
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• pp.202-206
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• 1995

In order to obtain the basic data for clarifing the mechanism of cold tolerance in crops, we analyzed various biochemical changes according to the Uniconazole treatment in Brassica seedling. Peroxidase activity in the root fraction of Brassica seedling was about 3 to 4 times higher than that in hypocotyl fraction, while catalase activity in those fractions showed opposite trend to the peroxidase activity. The content of hydrogen peroxide in root fraction was higher than that of hypocotyl fraction as being a reciprocal proportion with catalase activity. Especially in all fractions, peroxidase· activity of 'Sandongchae' (B. campestris) seedling, known as cold tolerant, was two-fold higher than that of cold sensitive rape(B. napus). The elongation rate of hypocotyl after germination was faster in B. napus than in B. campestris. The application of Uniconazole at 0.3 to 1.0 ppm to B. napus suppressed 43 to 46% of hypocotyl elongation and increased 65 to 73% of peroxidase activity in hypocotyl fraction. The shortening rate of hypocotyl length due to Uniconazole treatment was positively correlated with the increasing rate of peroxidase activity in hypocotyl fraction. Superoxide dismutase was not induced upon Uniconazole treatment and has only 3 isozymes in any fractions. Its activity was observed in the order of cotyledon>root>hypocotyl fraction.

• Current Research on Agriculture and Life Sciences
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• v.26
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• pp.71-79
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• 2008

It is known that the content of asparagine, an excellent detoxifying substance of alcohols in human body, is the highest in the roots of soy-sprout. At the same time, it is inferred that soy-sprouts producing more roots are better for detoxifying. In this experiment, the effects of room temperatures on number of watering per day, and duration of soy-sprout culture were carefully investigated. Some of the results obtained are as follows; 1. The yield rate of soy-sprouts for Agakong and Pungsannamulkong was continuously increased up to 9 days. It was higher in room temperature of $30^{\circ}C$ than in $20^{\circ}C$ and was the highest at 8th day of culture with 5 times of watering per day. 2. The asparagine content in soy-sprouts of Agakong and Pungsannamulkong was the highest in cotyledon and the lowest in roots. This rate was higher in the room temperature of $20^{\circ}C$ than in $30^{\circ}C$. 3. The highest asparagine content of soy-sprout of Agakong was 18.9%, obtained in the room temperature of $30^{\circ}C$, cultivated for 8 days with 5 times of watering per day. 4. The highest asparagine content of Pungsannamulkong was 18.8%, obtained in hypocotyl cultivated in the room temperature of $30^{\circ}C$ for 8 days with the number of 2 times watering per day. 5. When an cultivation apparatus of 5 liters volume was used, the optimum seed amount for the highest yield rate was 300g for Agakong and 500g for Pungsannamulkong. At the same time, the number of lateral roots showed increasing tendency with more amounts of soybean seeds used.

• Journal of Korean Society of Forest Science
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• v.34 no.1
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• pp.1-14
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• 1977

These studies were carried out to know the variation of morphological tree forms, needles, cotyledons, seed characters and top height growth of seedlings of Pinus koraiensis S. et Z. by populations in Mt. Jiri. The results are summarized as follows: 1. It was observed that the characteristics of length, needles of serration density of needles, percentage of full seeds length, of seeds, thickness, of seeds length of cotyledons, width of cotyledon, numbers of the stomata line in cotyledons and the top height growth of seedlings were significantly different in individual trees and populations. 2. The characteristics of branch angle specific gravity of wood and resin canal numbers of cotyledons were significantly different in populations. 3. It was that the characteristics of numbers of stomata line, width, of seeds weight, of seeds serration density of cotyledons, numbers of cotyledons were significantly different in individuals. 4. The resin canals of needles showed three principle resin canals in individual trees and population, and the coefficients of variation were not shown in individual trees and populations. Stem forms showed to be a straight form with all populations. 5. The characteristics of cotyledons had generally higher tendency than the characteristics of needles in comparison with coefficience of variation.

• Journal of agriculture & life science
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• v.45 no.4
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• pp.121-133
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• 2011

Effects of roasting on vitamin E content, color, microstructure and moisture of peanuts, and vitamin E content in peanut oils prepared from the roasted peanuts were investigated. Runner-type peanuts were roasted at 140, 150, and $160^{\circ}C$ for 10-20 min. As roasting temperature and time increased, the CIELAB $L^*$ value of peanuts decreased while $a^*$ and $b^*$ values increased, resulting in formation of the golden brown color of roasted peanuts. Moisture ratio (M/Mo) and color $b^*$ value of peanuts roasted at 140 to $160^{\circ}C$ showed a correlation of $b^*=21.61\;(M/Mo)^2-40.62\;(M/Mo)+34.12$ ($R^2=0.9123$). Overall changes in the tocopherol contents of peanuts and peanut oils were significantly affected by roasting temperature and time (p<0.05). Roasting at $140^{\circ}C$ caused a slight increase in the levels of tocopherols of peanuts over roasting time up to 20 min (p<0.05). There was no significant change in the tocopherol levels of peanuts during roasting at $150^{\circ}C$ for 20 min (p>0.05). At $160^{\circ}C$, the levels of tocopherols significantly decreased during the initial 10 min of roasting (p<0.05) while there was no extended loss after 10 min, resulting in about 5, 12, 20, and 10% losses of ${\alpha}$-, ${\beta}$-, ${\gamma}$- and ${\delta}$-T, respectively. After 20 min, total tocopherols decreased by 18%. However, tocopherol contents of pressed peanut oils significantly decreased at all roasting temperatures (p<0.05). After roasting peanuts at $160^{\circ}C$ for 20 min, about 84% of initial ${\alpha}$-T in peanut oils was retained. ${\alpha}$-T was the most stable to roasting while ${\gamma}$-T was the least. Swollen epidermal cells on the inner surface and broken cell walls of parenchyma tissue of peanut cotyledon were observed in peanuts after roasting at $160^{\circ}C$ for 15 min. Severe changes in microstructure of peanut by roasting would contribute to vitamin E stability because of exposure of oil droplets in peanuts to oxygen.

• Korean Journal of Breeding Science
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• v.40 no.1
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• pp.26-30
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• 2008

Normal soybean seed contains three lipoxygenase isozymes called L-1, L-2, and L-3, respectively, which are responsible for the generation of undesirable grassy-beany flavors. Simple and effective methods for the detection of lipoxygenase isozymes were developed in soybean seeds. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) has been tried in separating these isozymes. It was done effectively on 7.5% separating gel and 4.5% stacking gel. However, no reliable method has been developed specifically for separating L-3, L-13 and L-23. Visual judging methods were based on the bleaching activities of lipoxygenase in contact with methylene blue and ${\beta}$-carotene. Sodium linoleate bleaching method was adopted to determine L-1 and L-2. Carotene bleaching and spectrophotometric methods were used to determine L-3. These systems were very rapid within one minute, furthermore only required a small piece of cotyledon (below 10 mg) and the other part could be used for generation advance after analysis. It was demonstrated that 200 seed samples could be analyzed per day by one laboratory assistant. The combination of visual judging methods and electrophoresis is suitable for breeding programs. It took 6.5 hours for analysis of 100 seed samples by one person.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.3
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• pp.186-194
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• 1994

Lack of seedling vigor is considered a serious deterrent to use of broadleafed birdsfoot trefoil (Lorus comicul~rus L.). Our objectives were to compare early seedling growth of broadleafed birdsfoot trefoil cultivar "MO-20" and the large-seeded accession 302921 at $15^{\circ}C$ and $25^{\circ}C$. in controlled-environment chambers. Cabon dioxide exchange rate (CER) was measured in a closed or open system using infrared gas analysis. Dark respiration rate was measured manometrically. Net carbon accumulated per day and growth analysis of the seedlings were calculated. Initial seed mass of 302921 was 3.5 times larger and final cotyledon area was 2 times larger than those of MO-20, and early seedling growth was better. But, from 3 weeks after emergence until the end of the test period at $25^{\circ}C$, MO-20 showed higher CER per unit leaf area and faster dry weight accumulation than did 302921. Compared with $25^{\circ}C$, growth of MO-20 at $15^{\circ}C$ was suppressed more than that of 302921. Dark respiration rate of MO-20 was slightly higher than that for 302921, but not COz uptake per day for MO-20 was highest at$25^{\circ}C$ and lowest at $25^{\circ}C$. The relative growth rate (RGR) of MO-20 was higher than 302921 at $25^{\circ}C$ due to high net assimilation rate, but there was little difference in RGR between entries at $15^{\circ}C$.}C$.

• Food Science and Preservation
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• v.12 no.6
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• pp.522-528
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• 2005

Effects of packaging and storage temperature on the quality and shelf life of mungbean sprouts(vigna radiata (L.) Wilczek) were studied Mungbean sprouts were packaged in polypropylene films(PP) and oriented polypropylene films(OPP) with 200 g, 250 g, and 300 g and stored at $4^{\circ}C,\;8^{\circ}C$ and $12^{\circ}C$, respectively. The deterioration of quality of mungbean sprouts during storage was caused by wilting of hypocotyl, abscission of cotyledon and softening of tissue. Total weight loss never exceeded $1\%$ and no visible signs of shrivelling of mungbean sprouts were observed. At $4^{\circ}C,\;30{\mu}m$ of OPP film per 250 g mungbean sprouts provided the optimal atmosphere composition(i.e. $3\%\;\O_2\;and\;5\%\;CO_2$). A shelf life of 6 days was achieved with these conditions. Hardness of hypocotyl, when deterioration in freshness began, was about 1,027.2 g when considerably deteriorated Hunter b value was 13 in deteriorated hypocotyl, vs. 11 for hypocotyl of fresh mungbean sprouts was accelerated by fluctuating storage temperature by the increment of storage time. It also was found that the optimum shelf life period was estimated to be 6, 2 and 2 days for 4, 8 and $12^{\circ}C$, respectively.

• The Korean Journal of Ecology
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• v.13 no.3
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• pp.225-236
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• 1990

We studied that seeds of wild radish with different size of weight can be obtained for the purpose of differentiation and growth of these seeds by total growth periods. This study is practised a view of plant eco-physiological side and dry-matter production. Through whole growth periods, we knowthat seed weight of L plots was higher than that of M and S plots. In the 33th day after day after germination, growth values of L, M and S plots were 13.93, 7.77, and 4.61 g/plant, respectively. Growth of shoot (cotyledon and leaf) area and shoot weight were shown the similar trends with individual plant dry-amtter weight. In the 33th day after germination, shoot area of L, M and S plots were 21.55, 11.81 and 8.75 $cm^3$/plant and shoot dry weight of L, M and S plots were 10.83, 6.03, 3.50 g/plant, respectively. In the early stage of growth (the 10-14th day after germination), the values of RGR of seed weight of L, M, S plots were 0.2887, 0.2807 and 0.2365 g/g/day, in the late stage of growth, those values of RGR of L, M, S plote were 0.2721, 0.1716, 0.1727 g/g/day, respectively. In the early stage of growth (the 14th day after germination), the values of NAR or L, M and S plots were 0.1513, 0.1373, and 0.1094 g/$cm^2$/day, respectively. It is thought that seed weights were influenced seriously effect in the early stage of growth. The NAR value, in the late stage of growth (the-28-33th day after germination), of L, M and S plots were not large different as 0.1086, 0.1097 and 0.112 g/$cm^2$/day, respectively. The LAR values of M and S (2.0396, 2.1520 $m^2$/g) plots, in the early stage of growth, were higher than L (1.9037 $m^2$/g) plots. In the late stage of growth, the value of LAR of L plots were nearly same as M plots, but the value of S plots were higher than that of L and M plots (0.0458 $m^2$/g). And, in the early stage of growth, the values of SLA of L, M and S plots were 2.2123, 2.2107 and 2.8448 $m^2$/g, respectively. We measured the photosynthetic rates and the respiratory rates as the physiological characteristics of wild radish. In the early stage of growth, the photosynthetic rates of L, M and S plots were 12.4, 14.4, 9.8 $\mu$mol /$m^2$/sec and 9.4, 11.4, 9.8 $\mu$mol/$m^2$ /sec in the late stage of growth, respectively. In this result, it could be informed that photosynthetic rates in the late stage of growth were lower than the early stage. In the middle stage of growth, the respiratory rates were 0.793, $\mu$mol/$m^2$/min in shoot, and 3.28 $\mu$mol/$m^2$/min in root without relation to seed weight. The growth analysis of plants was used by Hunt & Parson s computer program (1974, 1981) and its results used this program were good. Therefore, in this sense, it could be expected that the computer program used and developed largely in researches of plant growth.

• Korean Journal of Food Science and Technology
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• v.39 no.5
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• pp.588-592
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• 2007

In this study, we evaluated the recovery effects of dietary fiber extracted from soybean hulls on diarrhea in rats. Diarrhea-induced Sprague-Dawley male rats were divided randomly into 4 groups and fed experimental diets for 24 hours. The diets, based on the AIN93G diet, were as follows: CON (control diet), S-COTL (67.2 g/kg soybean cotyledon fiber diet), S-HULL (59.6g/kg soybean hull fiber diet), CHI (55.6g/kg chicory fiber diet). The results showed significant (10-20%) reductions of fecal water content in the CON and S-HULL groups, as compared to the S-COTL and CHI groups. The change in serum osmolality, a measure of dehydration symptoms, was significantly reduced in CON and S-HULL as compared to the S-COTL and CHI groups. Based on the results, it is suggested that soybean hull fiber functions well for diarrhea recovery in rats. Consequently, soybean hull fiber is an important food source that could be used as a medical food in patients suffering from diarrhea.

• Research in Plant Disease
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• v.17 no.2
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• pp.191-195
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• 2011

This experiment was attempted to investigate a cause of the scion death in green pepper grafting system. A tobamovirus particle examined in the rootstock of the sample but not in the scion showing necrosis. The virus isolated from the rootstock was identified as Pepper mild mottle virus (PMMoV), pepper tobamovirus pathotype P1.2. (PMMoV-2), by nucleotide sequence analysis and host plant reaction. The virus isolate infected systematically in 6 commercial rootstock varieties using for green pepper grafting seedling production. Green pepper varieties 'Long green mart' and 'Daechan' represented resistance to the virus showing local lesions only on the inoculated leaves and 'Manitda' was systematically infected. In the experiment with grafting 'Long green mart' or 'Daechan' onto the those rootstocks, the upper leaves of the scions first showed vein necrosis and wilt symptoms 7 days after inoculation with PMMoV-2 on the cotyledon of the rootstock, following to the scion stem necrosis and then only the scion death. The virus was detected in the rootstock but not in the scion. However, 'Manitda' of susceptible variety in the grafting system showed mottle symptom on the leaves of the scion but not necrosis on the plant. PMMoV-3 isolate, pepper tobamovirus pathotype P1.2.3, did not cause the scion death in the grafting system. All of the varieties were susceptible to PMMoV-3. These results suggest that the scion death is caused by infecting with pepper tobamovirus pathotype P1.2. in the green pepper grafting system combined with the susceptible rootstock and the resistance scion to the virus pathotype.