• Journal of Photoscience
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• 제12권1호
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• pp.1-9
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• 2005

The ubiquitin E3 ligase COP1 (Constitutive Photomorphogenesis 1) is a protein repressor of photomorphogenesis in Arabidopsisplants, and it found in various organisms, including animals. The COP1 protein regulates the stability of many of the light-signaling components that are involved in photomorphogenesis and in the developmental processes. To study the effect of COP1 on flowering in a short day plant, we have cloned a full-length of PnCOP1 (Pharbitis nil COP1) cDNA from Pharbitis nil Choisy cv. Violet, and we examined its transcript levels under various conditions. A full-length PnCOP1 cDNA consists of 2,280 bp nucleotidesthat contain 47 bp of 5'-UTR, 232 bp of 3'-UTR including the poly (A) tail, and 1,998 bp of the coding sequence. The deduced amino acid sequence contains 666 amino acids, giving it a theoretical molecular weight of 75 kD and a isolectric point of 6.2. The PnCOP1 contains three distinct domains, an N-terminal $Zn^2+$-binding RING-finger domain, a coiled-coil structure, and WD40 repeats at the C-terminal, implying that the protein plays a role in protein-protein interactions. The PnCOP1 transcript was detected in the cotyledon, hypocotyls and leaves, but not in root. The levels of the PnCOP1 transcript were reduced in leaves that were a farther distance away from the cotyledons. The expression level of the PnCOP1 gene was inhibited by light, while the expression was increased in the dark. During the floral inductive 16 hour-dark period for Pharbitis nil, the expression was increased and it reached its maximum at the 12th hour of the dark period. The levels of PnCOP1 mRNA were dramatically reduced upon light illumination. These results suggest that PnCOP1 may play an important function in the floral induction of Pharbitis nil.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1994년도 Proceedings of International Symposium on BIOLOGICAL CONTROL OF PLANT DISEASES Korean Society of Plant Pathology
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• pp.27-49
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• 1994

Root colonization of biocontrol agents via seed treatment was investigated and a compatible combination, Gliocladium virens G872B and Pseudomonas putida Pf3, in colonizing cucumber rhizosphere was confirmed through the study. Much higher number of fungal and bacterial propagules were detected when two isolates were inoculated together. The presence of Pf3 in root system was greatly helpful to G872B to colonize at root tip. The mechanism of this phenomenon is partially elucidated through the results of in vitro experiments and the observations of scanning electron and fluorescence microscope. Addition of Pf3 cells resulted earlier germination of G872B conidia and increased mycelial growth. And the more number of germinated conidia on seed coat, the more vigorous hypal streching and sporulation on the root surface were observed in coinoculated treatment. The propagules of G872B on the cucumber root when they were challenged against the pathogenic Fusarium oxysporum, were even higher than that of G872B treated alone, and the magnitude of such a difference was getting grater toward the root ip and the population of F. oxysporum on the root was reduced by seed inoculation of G872B. The rhizosphere competence was obviously reflected to disease suppression and plant growth promotion that induced by the given isolate. Green house experiments revealed that the combined treatment provided long-term disease suppression with greater rate and the larger amount of fruit yield than single treatments. Through this study the low temperature growing Pseudomonas fluorescens M45 and MC07 were evaluated to apply them to the winter crops in field or plastic film house. In vitro tests reveal that M45 and MC07 inhibited the mycelial growth of Pythium ultimum, Rhizoctona solani and Phytophthora capsici and enhanced growth of cucumber cotyledon in MS agar. This effect was more pronounced when the bacteria were incubated at 14$^{\circ}C$ than at 27$^{\circ}C$. And disease suppression and plant growth promotion in green house were also superior at low temperature condition. Seed treatment of M45 or soil treatment of MC07 brought successful control of damping-off and enhanced seedling growth of cucumber. The combined treatment of two isolates was more effective than any single treatment.

• Current Research on Agriculture and Life Sciences
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• 제16권
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• pp.31-36
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• 1998

어수리의 미숙배로부터 체소포배 형성시 자엽의 수적변이에 미치는 cytokinin의 영향을 조사하하였다. 구상형배에 0.2mg/l의 BA, 2ip, kinetin을 각각 처리하여 2주간 배양하였을 때, 다자엽의 형성률은 각각 84.9, 42.6, 32.9%로서 대조구(25.2%)에 비하여 BA>2ip>kinein순으로 높았으며 특히 BA 처리시에 가장 높게 나타났다. 또한 BA를 농도별(0, 0.01, 0.05, 0.1mg/l)로 배발생세포괴에 2주간 처리하였을 때, 다자엽의 형성율은 각각 23.3, 39.7, 69.4, 74.4%로서 농도가 높을수록 증가하는 경향이었다. 0.2mg/l의 BA를 구상형, 심장형, 또는 어뢰형, 자엽시기의 배에 2주간 처리한 경우에 다자엽의 형성률은 각각 92.4, 84.4, 26.3%로서 배발생 초기에 처리될 경우에 더욱 높게 나타났다.

• Plant Resources
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• 제2권1호
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• pp.1-9
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• 1999

The callus from the hypocotyl region of immature embryo of Citrus junos Sieb. was efficiently induced in the $\frac{1}{2}$ MS medium containing 45uM BA after 8 weeks culture. The callus was developed into the two callus type, embryogenic callus and nonembryogenic callus, which can be distinguished by visual examination depending on color and appearence. In vitro regeneration of callus established efficiently in the hormone-free MS medium from the embryogenic callus. In order to investigate the physiological changes depending on the developmental stage of embryo, the embryo was formed in the MS medium. The embryogenic and nonembryogenic callus, and the various stages of the somatic embryo were examimed the changes of esterase activity, and their isozyme patterns as well. The protein content and esterase activities was gradually increased on the developmental stages of embryo. Total protein pattern were different by the SDS-PAGE and were appeared strong band of 23 KD in the torpedo stage. The pattern of the esterase isozyme was exhibited a difference between embryogenic callus and nonembryogenic callus. It was appered pI 6.0, 8.0, 8.2 in the embryogenic callus. Also the new band of pI 4.75 was appeared in the cotyledon. These results suggest that the changes of esterase activities and isozyme patterns are importent factor in the differentiation and development of citrus.

• 한국환경과학회지
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• 제28권3호
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• pp.385-392
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• 2019

This study investigated the relationship among seven species of trichoderma through the identification of strains collected in Korea. The phylogenetic tree among the collected strains was classified into four groups. The trichoderma strains isolated in this way showed inhibitory effect on the fusarium wilt which is parasitic to cotyledon stem..The invisibility of J9, J10, J13 and J16 strains were higher in comparison with other strains in vitro test stand, and their spore production level was also higher. In the aluminum ring tests, it showed that the yield of the spores in J9, J10 and J13 were more than any other strain. As a result conducting the port test for cucumbers, the plant lengths of J13 were larger than the control plot, and the root lengths of all strains, except for J2 were longer than the control plot, and the root weights of J1, J9, J10, J13 and J16 were larger than the control plot. The disease severity for the fusarium wilt showed the smallest values at J13 and J16 in comparison with the control plot, and the control values of J13 and J16 were higher than other strains.

• 한국작물학회지
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• 제39권3호
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• pp.211-215
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• 1994

Lipoxygenase는 콩 비린내 유발의 주원인이 된다. 따라서 lipoxygenase 발현 양상을 알아보고자 콩 생육시기별로 재료를 채취하여 lipoxygenase activity를 측정한 바 그 결과는 다음과 같다. 1. $V_3$ 와 $V_5$시기에 콩 잎에서의 lipoxygenase activity는 유전자형에 상관없이 일정한 경향이 없었다. 2. $R_6$시기의 /pod wall에서도 유전자형에 따른 lipoxygenase activity의 일정한 경향은 없었으나 종자 lipoxygenase는 lipoxygenase 결핍계통의 activity가 일반품종에 비해 낮았으며 lipoxygenase 결핍계통간에도 isozyme 결핍정도에 따라 activity도 다르게 나타났다. 3. 종자를 종피, 배, 자엽으로 구분하여 각각의 lipoxygenase activity를 측정한 결과 종피에서는 유전자형에 따른 경향을 찾을 수 없었으나 배와 자엽에서는 lipoxygenase isozyme 결핍 정도에 따라 activity도 다르게 나타났다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.148.2-149
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• 2003

Cucumber fruit mottle mosaic tobamovirus (CFMMV) causes severe mosaic symptoms with yellow mottling on leaves and fruits, and occasionally severe wilting of cucumber plants. No genetic source of resistance against this virus has been identified. The genes coding for the coat protein or the putative 54-kDa replicase were cloned into binary vectors under control of the SVBV promoter. Agrobacterium-mediated transformation was peformed on cotyledon explants of a parthenocarpic cucumber cultivar with superior competence for transformation. R1 seedlings were evaluated for resistance to CFMMV infection by lack of symptom expression, back inoculation on an alternative host and ELISA. From a total of 14 replicase-containing R1 lines, 8 exhibited immunity, while only 3 resistant lines were found among a total of 9 CP-containing lines. Line 144 homozygous for the 54-kDa replicase was selected for further resistance analysis. Line 144 was immune to CFMMV infection by mechanical and graft inoculation, or by root infection following planting in CFMMV-contaminated soil. Additionally, line 144 showed delay of symptom appearance following infection by other cucurbit-infecting tobamoviruses. Infection of line 144 plants with various potyviruses and cucumber mosaic cucumovirus did not break the resistance to CFMMV. The mechanism of resistance of line 144 appears to be RNA-mediated, however the means is apparently different from the gene silencing phenomenon. Homozygote line 144 cucumber as rootstock demonstrated for the first time protection of a non-transformed scion from soil inoculation with a soil borne pathogen, CFMMV.

• 분석과학
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• 제22권4호
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• pp.277-284
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• 2009

원적외선 처리수와 음용수에서 자란 무의 발아율은 상대적으로 100%와 78% 이었다. 원적외선 처리수는 암소와 광에서 자엽의 세포분열을 촉진시켰고, 세포크기도 증가시켰다. 암소와 광조건에서, 원적외선 처리수에서 성장한 자엽의 종축과 횡축의 크기는 음용수에서 성장한 자엽보다 더 켰다. 엽록소의 양과 $CO_2$ 소모량은 원적외선 처리수에서 자란 자엽에서 상대적으로 더 높았다. 삼투압은 원적외선 처리수에서 성장한 자엽이 음용수에서 성장한 것보다 1.25배 더 높게 측정되었고, 수분퍼텐셜은 원적외선 처리수에서 자란 자엽이 더 음의 값을 보였다. 원적외선 처리수에서 성장한 하배축의 길이는 음용수보다 암소조건에서는 2.18배, 광조건하에서는 1.99배 더 키가 컸고, 무의 크기가 상대적으로 더 켰다.

• 한국작물학회지
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• 제51권spc1호
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• pp.160-165
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• 2006

우수한 기능성을 가진 나물콩을 생산하기 위하여 isoflavone함량이 높은 품종을 선발하고, 재배적 방법에 의한 원료콩 종실의 isoflavone함량의 증진 가능성을 조사하는 한편, 콩나물의 재배 일수에 따른 콩나물 부위별 isoflavone 함량의 경시적 변화 양상을 조사한 결과를 요약하면 다음과 같다. 1. 공시한 나물콩 21품종의 종실에 함유된 daidzein과 genistein의 총 함량은 $756{\sim}1,682{\mu}g/g$으로 품종간 차이가 컸으며, 특히 익산 13호$(1,682{\mu}g/g)$ 및 남원재래$(1,500{\mu}g/g)$에서 함량이 높았다. 2. Daidzein 함량은 익산 13호$(924{\mu}g/g)$ 및 IT 160996 $(828{\mu}g/g)$에서 높았으며, genistein 함량은 익산 13호$(758{\mu}g/g)$ 및 남원재래$(756{\mu}g/g)$에서 높았다. 3. 풍산나물콩의 영양생장기에 식물생장조절제인 안티폴$(2,472{\mu}g/g)$과 후라스타$(2,052{\mu}g/g)$를 처리하였을 때 daidzein과 genistein의 총 함량이 대조군$(1,800{\mu}g/g)$에 비하여 각각 37%와 14% 증가하였다. 4. 은하콩의 콩나물 재배일수 경과에 따른 isoflavone 함량은 자엽의 경우 변화가 미미하였으나, 배축의 경우 daidzein이 치상 후 3일까지 증가하다 4일째부터 급격히 감소하는 경향을 나타냈으며, 배축의 genistein함량은 콩나물 재배기간이 경과할수록 감소하였다. 5. 풍산나물콩의 콩나물 재배일수 경과에 따른 isoflavone 함량은 은하콩과 달리 자엽의 경우에는 변화가 미미하였으나, 배축의 경우 daidzein이 치상 후 1일에 $5,154{\mu}g/g$까지 증가했으나 재배일수가 경과함에 따라 급격히 감소하는 경향을 나타냈으며, 배축의 genistein함량은 큰 변동을 보이지 않았다. 6. 콩나물재배 6일째부터 발생한 잔뿌리에는 isoflavone 함량이 $4,416{\mu}g/g{\sim}5,232{\mu}g/g$으로 매우 높았다. 7. 고기능성 나물용 콩을 육성하기 위해서는 원료콩 종실의 isoflavone함량이 높고, 콩나물이 식용으로 이용되는 시기까지 isoflavone함량이 크게 감소하지 않는 특성을 가진 품종의 육성이 필요할 것으로 생각된다.

• 한국작물학회지
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• 제56권4호
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• pp.394-399
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• 2011

땅콩의 새로운 수요개발을 위해 국내 육성품종 37종에 대한 땅콩 싹나물 비교시험을 통하여 싹나물에 적합한 우량품종을 선발하고 생육형질 상호간의 관계를 조사여 싹나물 연구를 위한 기초 자료자료로 활용하고자 수행한 결과는 다음과 같다. 본 시험에 이용한 각 품종의 100립중은 조광 56 g에서 호광 142 g까지 넓게 분포하였다. 전체품종의 하배축길이는 팔광 4.3 cm에서 대신 5.8 cm 분포하고 평균 5.1 cm였으며 하배축의 직경은 평균 6.9 mm 미광 5.0 mm에서 대청 8.0mm까지 걸쳐 분포하였다. 뿌리길이는 대청 11.1 cm에서 미광 20.6 mm에 분포하였으며 평균 14.6 cm이였다. 상배축의 길이는 평균 1.8 cm로서 대풍 0.8 cm에서 미광 4.6 cm에 걸쳐 분포하였다. 미광은 하배축이 가늘고 짧으며, 상배축과 뿌리가 가장 길었고 기능성 성분 resveratrol 함량($88.2\;{\mu}g/g$)도 높았다. 중소립종 조광은 하배축의 길이(5.0 cm)가 짧은 편이나 하배축 직경(7.8 mm)은 통통하고 싹나물의 크기가 균일하며 수율은 820%로 가장 높았으며 특히 깨끗한 외관 품질을 가져 땅콩 싹나물로 적합한 특성을 가졌다. 땅콩 싹나물 형질 상호간의 상관관계에서 땅콩 종자의 100립중은 싹나물의 resveratrol함량, 성묘율, 수율과는 부(-)의 상관관계를 나타냈고, 묘중, 곡묘 하배축 개열성과는 정(+)의 상관관계를 나타내었다. 또한 싹나물 뿌리 길이는 상배축의 길이, resveratrol 함량, 수율과는 정의 관계를 나타내고 하배축 직경과 개열성과는 부의 관계를 나타냈다.