• 제목/요약/키워드: cos

검색결과 492건 처리시간 0.034초

The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes

  • Tsuzuki, Y.;Nozawa, K.;Ashizawa, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제22권3호
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    • pp.328-335
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    • 2009
  • This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs) immature porcine oocytes. The in vitro nuclear maturation of the COs, exposed to and removed from the CPAs was also assessed. Both COs and DOs were exposed to 1.5 M concentrations of each CPA (ethylene glycol (EG); propylene glycol (PG); dimethyl-sulfoxide (DMSO); and glycerol (G)) for durations of 5, 15, and 30 minutes at room temperature ($23.5{\pm}1.5^{\circ}C$), and immersed in physiological saline supplemented with 20% (v/v) fetal bovine serum for 5 minutes ($39^{\circ}C$) to remove each CPA. Before, during and after exposure to each CPA, the ATP content of both the COs and the DOs was measured. After removal from each CPA an aliquot of the COs was matured for 44${\pm}$2 h, and their nuclear maturation rates were measured up to the metaphase stage of the second meiotic division (the M-II stage). The maturation rates up to the M-II stage were not significantly different between all the groups that were exposed to each CPA for 5 minutes. For 15 and 30 minute exposures, the maturation rates of the COs exposed to PG, DMSO and EG were almost the same as those of the control groups; however, the rates of G group exposed for 15 and 30 minutes were significantly lower (p<0.05) than the control group. These groups were also found to have a decrease in the ATP content of COs and DOs during and after exposure for the same periods (p<0.05). In addition, although the ATP contents of the COs after exposure to EG for any period were the same as the controls, the ATP content of the DOs exposed to EG for any period were significantly lower (p<0.05) than those of the controls. When the ATP content of the COs and DOs of each CPA were compared, the DOs exposed to PG were found to have a significantly greater level (p<0.05) than DOs exposed to G for any duration. In addition, the ATP content of DOs exposed to PG for 30 min and removal was also higher (p<0.05) than when exposed to DMSO for the same period. These findings indicate that PG may be a useful CPA for the cryopreservation of immature porcine oocytes.

Effect of Chito-oligosaccharide Supplementation on Immunity in Broiler Chickens

  • Deng, Xingzhao;Li, Xiaojing;Liu, Pai;Yuan, Shulin;Zang, Jianjun;Li, Songyu;Piao, Xiangshu
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권11호
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    • pp.1651-1658
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    • 2008
  • This study was conducted to determine the effects of dietary supplementation of either 100 mg/kg chito-oligosaccharide (COS) or chlortetracycline (CTC) with corn-soybean-fish meal on immunity in broiler chickens. A total of 147 one-day old male broiler chicks were randomly allocated to 3 treatments with 7 replicate pens per treatment and 7 birds per pen. The experimental diets consisted of a control diet based on corn, soybean and fish meal without COS and any antibiotic supplement and similar diets supplemented with either CTC (80 mg/kg from d 1 to 21 and 50 mg/kg from d 22 to 42) or COS (100 mg/kg from d 1 to 42). During the entire experimental period, all birds had ad libitum access to diets and water. The main immune organ indices, T-lymphocyte proliferation, serum cytokine concentrations, serum NO level and serum iNOS activity were measured on d 21 and d 42. On d 21, broilers fed 100 mg/kg COS had improved (p<0.01) indices of spleen, thymus, and bursa of Fabricius compared with the control and CTC birds. Birds receiving 100 mg/kg COS had higher (p<0.05) serum concentrations of $IL-1{\beta}$, IL-6, IgM, NO and iNOS than birds on the control treatment. Serum $Ca^{2+}$ level of birds fed 100 mg/kg COS tended to be higher (p = 0.049) than in birds fed CTC. On d 42, the birds fed 100 mg/kg COS had higher (p<0.05) concentrations of TNF-${\alpha}$ and IgM in serum than birds in both the CTC and control treatments. Birds fed 100 mg/kg COS had a higher concentration of IFN-$\gamma$ than the control group. In conclusion, dietary supplementation of COS appeared to improve the immunity of broilers by promoting the weight of the main immune organs, increasing IgM secretion, stimulating microphages to release $TNF-{\alpha}$, $IL-1{\beta}$, IL-6 and IFN-$\gamma$, and activating iNOS to induce NO.

Bacillus subtilis koji와 Rhizopus oryzae koji를 이용한 된장 및 간장의 키토올리고당 함량 증대 (Enhancement of Chitooligosaccharides in Doenjang (Soybean Paste) and Kanjang (Soy Sauce) using Bacillus subtilis Koji and Rhizopus oryzae Koji)

  • 음병욱;곽보연;김순영;손동화;이계호
    • 한국식품과학회지
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    • 제35권2호
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    • pp.291-296
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    • 2003
  • 된장 및 간장의 chitooligosaccharide(COS) 함량을 높이기 위해 Bacillus subtilis와 Rhizopus oryzae를 접종하여 koji를 만들고 이들 균주들에 의한 된장 및 간장에서 COS 함량변화에 대하여 알아보았다. COSM에 특이적인 항체를 이용하여 competitive direct ELISA(cdELISA)로 COS를 검출하였는데, 검출 감도는 $0.001{\sim}1{\mu}g/mL$이었고, 회수율은 된장 및 간장에서 각각 102%, 115%를 보였다. B. subtilis koji와 R. oryzae koji를 섞어 담근 된장 또는 간장(BR 된장 또는 간장)이 높은 COS 함량을 보였는데, 두 달 숙성에서 된장이 $171{\mu}g/g$, 간장이$29{\mu}g/mL$을 나타내었다. 일본, 국내 시판, 그리고 실험실에서 만든 된장 및 간장의 COS 함량을 비교해 본 결과, BR 시료구가 일본이나 국내 시판용보다 훨씬 높은 함량을 나타내 주어서 본 실험에서 koji 제조시 이용된 Bacillus subtilis와 Rhizopus oryzae 의해 COS의 함량을 높일 수 있는 가능성을 보여주었다.

New Serial and Parallel Sin+Cos PSS1A PSS Design and Analysis

  • Lee Sang-Seung;Li Shan-Ying;Jang Gwang-Soo;Park Jong-Keun;Moon Seung-Il;Yoon Yong-Tae
    • KIEE International Transactions on Power Engineering
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    • 제5A권4호
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    • pp.378-384
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    • 2005
  • This paper proposes a new series and parallel Sin+Cos PSS (power system stabilizer) for the purpose of improving the existing PSS1A's performance. The purpose of the PSS is to enhance the damping of power system oscillations through injection of auxiliary signals for an excitation control terminal. The proposed series and parallel Sin+Cos PSS is connected adding the Sin+Cos terms additionally with the serial and parallel connection in a conventional PSS1A. The proposed controller is aimed at considering the damping of oscillation when it changes parameter fluctuations or operational load variations in a power system. The electric power system used is the KEPCO system and the voltage of the power transmission line is 154kV and 345kV. The PSCAD/EMTDC package is used to authorize the effect of the proposed controller. Simulations were shown by and compared with the waveforms for frequency, voltage and electric power.

한국 근해 수온의 주기적 변화(I) 남해의 거문도해역 표면수온 년주변화 및 영년변화 (Periodic Variations of Water Temperature in the Seas Around Korea(I) Annual and Secular Variations of Surface Water Temperature, Kumun-Do Region, Southern Sea of Korea)

  • 한상복
    • 한국해양학회지
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    • 제5권1호
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    • pp.6-13
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    • 1970
  • Ten days and monthly mean temperatures were analysed daily data observed during July, 1916 to March, 1970 statistically. Periodic characters were calculated by Δn, new method of approximate solution of Schuster Method. According to ten days mean temperatures, annual variation function is F($\theta_d$)=16.29-5.27 cos $\theta_d$+0.75 cos2 $\theta_d$-3.14 sin $\theta_d$+1.16 sin2 $\theta_d$-0.63 sin $\3{theta}_d$, where $\theta_d$=$-\frac{\pi}{18}$(d-3), d is the order of ten days period, 1 to 36. Annual mean water temperature is 16.3$^{\circ}C$, minimum in the last ten days of February 10.9$^{\circ}C$, maximum in the last ten days of August 24.5$^{\circ}C$. Periodic character of secular variation shows 11 year and its curve is F($\theta_y$)=16.29+0.53 cos $\theta_y$ -0.16cos $2{\theta}_y$+0.10 cos$3{\theta}_y$-0.10 sin $\theta_y$, where $\theta_y$=2$-\frac{2\pi}{11}$(y-1920), y is calendar year. And the relation between air temperature x and water temprature y is following. y=9.67 1.035$\^x$

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블록 암호 SCOS-3의 연관키 차분 공격에 대한 안전성 분석 (Security Analysis of SCOS-3 Block Cipher against a Related-Key Attack)

  • 이창훈
    • 한국항행학회논문지
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    • 제13권6호
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    • pp.977-983
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    • 2009
  • 최근, 하드웨어 환경에서 효율적으로 구현이 가능한 DDP, DDO, COS-기반 블록 암호에 대한 연구가 활발히 진행되고 있다. 그러나 대부분의 DDP, DDO, COS-기반 블록 암호들은 연관키 공격에 취약한 것으로 드러났다. 12-라운드 블록 암호 SCOS-3는 기제안된 DDP, DDO, COS의 취약점을 제거하기 위해 개발된 COS-기반 블록 암호이다. 본 논문에서는 SCOS-3의 11-라운드 축소 버전에 대한 연관키 차분 공격을 제안한다. 본 논문에서 소개하는 공격은 SCOS-3에 대한 첫 번째 공격이며, $2^{58}$개의 연관키 선택 평문을 이용하여 $2^{117.54}$의 11-라운드 SCOS-3 암호화 연산을 수행하여 11-라운드 SCOS-3의 비밀키를 복구한다. 이를 통해 SCOS-3가 여전히 연관키 공격에 취약함을 알 수 있다.

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고전압 COS 퓨즈로부터 방사된 충격성 소음 저감용 반사형 소음기의 설계 (Reactive Silencer Design for Suppressing Impulse Noise from a High Voltage Cut Out Switch Fuse)

  • 송화영;김덕한;이종석;이동훈
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2006년도 춘계학술대회논문집
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    • pp.951-954
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    • 2006
  • This Study introduces the design of a reactive type silencer for reducing impulse noises emitted from a high voltage COS fuse of a transformer. When a high voltage COS fuse becomes a short circuit by the over current, the peak sound pressure level above 150dB(A) is generated at the distance of 2m from a COS Fuse. For the purpose of the reduction of impulse noise, in this study. the reactive type silencers have been utilized. The performance of noise reduction for them can be expressed by insertion loss. The reactive silencers have been tested for 23 different types with each different porosity, hole diameter and depth. From the experimental results, it is found that the reactive silencer has an excellent performance to greatly suppress the impulse noise. The one-stage, two-stage and three-stage reactive type silencers have been shown to have the insertion losses of about from 35dB (A) to 25dB(A).

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뉴로 네트워크에서 코사인 모듈화 된 가우스함수의 다항식과 계단함수의 근사 (Approximation of Polynomials and Step function for cosine modulated Gaussian Function in Neural Network Architecture)

  • 이상화
    • 전자공학회논문지CI
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    • 제49권2호
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    • pp.115-122
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    • 2012
  • 본 논문에서는 CosGauss라고 하는 코사인함수로 모듈화 된 가우시안 활성화함수가 뉴로 네트워크에서 다항식과 계단함수의 근사에 사용될 수 있음을 증명한다. CosGauss 함수는 시그모이드, 하이퍼볼릭 탄젠트, 가우시안 활성화 함수보다 더 많은 범프(bump)를 구성 할 수 있다. 이 함수를 캐스케이드 코릴레이션 뉴로 네트워크 학습에 사용하여 벤치마크 문제인 Tic-Tac-Toe 게임과 아이리스(iris) 식물 문제와 실험하고 여기에서 얻어진 결과를 다른 활성화 함수를 사용한 결과와 비교 분석한다.

실시간 임베디드 운영체제 TMO-eCos의 데드라인 기반 CPU 소비 전력 관리 (A Deadline_driven CPU Power Consumption Management Scheme of the TMO-eCos Real-Time Embedded OS)

  • 박정화;김정국
    • 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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    • 제15권4호
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    • pp.304-308
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    • 2009
  • 본 논문은 실시간 임베디드 OS인 TMO-eCos의 데드라인 기반 CPU 저전력 관리 기법을 다루고 있다. 해당 저전력 관리 기법은 경성 실시간 시스템인 TMO 시스템을 위한 태스크 순차화 기법에서 도출된 스케줄링 시나리오를 사용한다. 본 연구팀에서 개발한 스케줄링 사전 분석기는 주기적으로 동작하는 태스크의 주기, 데드라인, WCET를 기반으로 오프라인 분석을 실시한다. 최종적으로 TMO-eCos 커널은 CPU의 전력 소모를 줄이기 위하여 주기적인 태스크의 데드라인을 위반하지 않는 범위에서 CPU의 속도를 조절하여 시스템에서 사용하는 소비전력은 줄이게 된다. 본 논문은 이와 같은 과정과 실제 실험결과를 기술한다.