• 대한바이러스학회지
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• 제30권1호
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• pp.83-99
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• 2000

A defective HIV-1 helper virus DNA, pHyPC, was assembled by deleting the RNA packaging signal, env, nef and the 3'LTR sequences. HIV-1 like virus particles that carry the HIV-1 receptor, CD4 were generated by co expression of pHyPC and plasmid DNAs encoding different chimeric CD4 proteins. The CD4 particles, sharing the CD4 ectodomain, precisely fused to different membrane anchors. CD4(+) particles specifically bound to HIV-1 Env expressing cells, but any signs of infection into these cells were not detected. Binding was only partially blocked by either polyclonal anti-CD4 antibodies or by high concentrations of soluble CD4. Surprisingly, CD4(+) particles also adsorbed to HeLa, CHO, NIH3T3 and COS-7 cells in the absence of HIV-1 Env expression. Adsorption was comparable in strength and speed to the highly specific CD4-Env interaction. CD4(-) particles exhibited only background levels of binding. Cell binding was CD4. dependent, but it was independent of the cell type from which the CD4(+) particles originated. Interestingly, CD4-dependent/Env-independent binding was only found when CD4 was present on virus particles. This suggests that the micro-environment of CD4 on virus particles uniquely expose this new cell binding activity. Its high affinity could explain in part why infection of Env(+) cells by CD4(+) particles was not detected. Further experiments will be required to evaluate whether this strong membrane interaction could represent one step in the multiple-step viral entry process.

• Molecules and Cells
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• 제28권1호
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• pp.25-30
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• 2009

${\beta}$-Arrestins turn off G protein-mediated signals and initiate distinct G protein-independent signaling pathways. We previously demonstrated that angiotensin $AT_1$ receptorbound ${\beta}$-arrestin 1 is cleaved after $Phe^{388}$ upon angiotensin II stimulation. The mechanism and signaling pathway of angiotensin II-induced ${\beta}$-arrestin cleavage remain largely unknown. Here, we show that protein Tyr phosphatase activity is involved in the regulation of ${\beta}$-arrestin 1 cleavage. Tagging of green fluorescent protein (GFP) either to the N-terminus or C-terminus of ${\beta}$-arrestin 1 induced conformational changes and the cleavage of ${\beta}$-arrestin 1 without angiotensin $AT_1$ receptor activation. Orthovanadate and molybdate, inhibitors of protein Tyr phosphatase, attenuated the cleavage of C-terminal GFP-tagged ${\beta}$-arrestin 1 in vitro. The inhibitory effects of okadaic acid and pyrophosphate, which are inhibitors of protein Ser/Thr phosphatase, were less than those of protein Tyr phosphatase inhibitors. Cell-permeable pervanadate inhibited angiotensin II-induced cleavage of ${\beta}$-arrestin 1 in COS-1 cells. Our findings suggest that Tyr phosphorylation signaling is involved in the regulation of angiotensin II-induced ${\beta}$-arrestin cleavage.

• 한국통신학회논문지
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• 제30권11A호
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• pp.979-986
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• 2005

광전달망(Optical Transport Network)에서 사용자의 서비스 요구는 종종 주기적인 특성을 보이므로 이러한 계획 연결 요구에 대하여 시간 중복성을 RWA 문제에 이용할 수 있다. 계획 연결 요구의 RWA 문제는 조합 최적화 방법(Combinatorial Optimal Solution)과 그래프 컬러링(Graph Coloring)의 조합으로 해결되거나, 경로 선택에 기반한 순차적(sequential) RWA(sRWA) 의해서 해결된다. 이와 같은 방법은 복잡하거나 많은 연산을 필요로 하므로 본 논문에서는 그룹화를 통해 계획 연결 요구들의 시간 중복성을 이용하여 효과적으로 경로 설정 및 파장 할당을 하는 알고리즘을 제안한다. 본 논문에서 제안하는 알고리즘이 현재 실질적으로 효율적이라 알려진 sRWA 보다 같거나 작은 파장 수를 사용하여 $54\%$ 이상의 계산량의 성능 향상을 보인다.

• 한국컴퓨터정보학회논문지
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• 제8권1호
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• pp.51-59
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• 2003

분산 객체 복제 시스템에서 노드들이 공유하는 객체는 동일 내용을 복수 노드에 위치시키는 것이 효율적이다. 노드들은 시스템에 접근시 접근 정보를 자신의 지역 캐시에 저장해 두었다가 필요시에 인출해서 사용한다. 그러나 시간이 지나감에 따라 다른 노드들에 의해서 데이터의 갱신이 이루어지기 때문에 일관성 문제가 발생한다. 따라서 시스템의 일관성 유지를 통해 성능 및 가용성을 높이기 위해서는 객체를 효율적으로 관리하는 메커니즘이 필요하다. 본 논문에서는 공유 메모리 환경에서 일관성 유지를 위해 객체 관리시 기존의 중복 기법에서 사용하는 일관성 비용 외에 부가 비용이 없이도 제한적으로 병렬 수행의 효과를 얻으며, 또한 중복 기법에서 가장 큰 오버헤드로 알려진 일관성 유지비용을 최소화시키기 위하여 이 비용을 결정하는 가장 핵심적인 요소인 객체 복제본의 수와 위치 그리고 각 객체 사이의 상관도를 고려하여 객체를 효율적으로 관리하고, 전체 수행 시간을 개선시키는 적응적 중복 객체 관리 메커니즘을 연구한다.

• Molecules and Cells
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• 제19권1호
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• pp.39-45
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• 2005

RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.

• 대한기계학회논문집
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• 제14권3호
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• pp.725-733
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• 1990

본 연구에서는 Raju등의 연구의 확장으로 일정한 온도로 유지되는 경사진 평 판에 대하여 혼합대류 전 영역에 유효한 부력계수(.xi.)로서 (1+Gr$^{2}$$_{x}$cos.gamma./ Re$^{4}$$_{x}$)$_{-1}$를 적용하여 비상사 경계층 방정식을 유도하였다. 또한 적분 형 경계조건을 가진 편미분 경계층방정식을 유한차분법으로 Keller와 Cebeci의 Box scheme을 6차로 적용하여 수치해석하였다. 부력계수가 큰 영역에서 Mucoglu등의 해 석적 연구결과는 일정한 온도와 45˚의 경사를 유지하는 평판에 대한 Ramachandran의 실험적 연구결과와 약 10%정도 벗어난다. 이에 Ramachandran의 실험적 연구를 해석 적으로 재고찰하였다.하였다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.43-43
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• 2003

Large-conductance $Ca^{2+}$-actived $K^{+}$ channels ($BK_{Ca}$ channels) play a key role in setting the pace of contractile activity in muscle and are involved in the regulation of neurotransmitter release in neuron. $BK_{Ca}$ channels are activated by depolarizing membrane potential and the elevated level of intracellular calcium. Using yeast-two hybrid assay, we have identified a novel protein interacting with the cytosolic carboxyl terminus of rSlo, the brain isoform of rat large-conductance $Ca^{2+}$-activated $K^{+}$ channel $\alpha$-subunit. The novel gene encodes 51 kDa protein and is named as SIRK(rSlo-interacting RGS-like protein). SIRK is expressed in various tissues and localized in the cytosolic and the membrane fraction. Biochemical and immunological studies indicated that SIRK physically interacted with the cytosolic region of rSlo. To investigate whether SIRK can modulate the activity of rSlo, GFP-fused SIRK and rSlo were transiently transfected into COS-7 cells and the effects of SIRK was studied using electrophysiological means. We concluded that the overexpression of SIRK alters the surface expression of rSlo channel with only a limited effect on the biophysical characteristics of the channel.the channel.

• Journal of Electrical Engineering and Technology
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• 제7권1호
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• pp.1-8
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• 2012

The current paper presents a novel control strategy of a three-phase, four-wire Unified Power Quality (UPQC) to improve power quality. The UPQC is realized by the integration of series and shunt active power filters (APF) sharing a common dc bus capacitor. The realization of shunt APF is carried out using a three-phase, four-leg Voltage Source Inverter (VSI), and the series APF is realized using a three-phase, three-leg VSI. To extract the fundamental source voltages as reference signals for series APF, a zero-crossing detector and sample-and-hold circuits are used. For the control of shunt APF, a simple scheme based on the real component of fundamental load current (I $Cos{\Phi}$) with reduced numbers of current sensors is applied. The performance of the applied control algorithm is evaluated in terms of power-factor correction, source neutral current mitigation, load balancing, and mitigation of voltage and current harmonics in a three-phase, four-wire distribution system for different combinations of linear and non-linear loads. The reference signals and sensed signals are used in a hysteresis controller to generate switching signals for shunt and series APFs. In this proposed UPQC control scheme, the current/voltage control is applied to the fundamental supply currents/voltages instead of fast-changing APF currents/voltages, thus reducing the computational delay and the required sensors. MATLAB/Simulink-based simulations that support the functionality of the UPQC are obtained.

• 한국정보처리학회논문지
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• 제7권5S호
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• pp.1666-1675
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• 2000

본 논문에서는 전자상거래 시스템에서 요구하는 상호운용성과 실시간 요구사항들을 지원할 수 있는 실시간 웹 미들웨이(RTWM Real-Time Web Middleware) 프레임워크를 제안한다. RTWM시스템은 전자상거래 시스템의 실시간 요구사항들을 지원하기 위해CORBA 객체 서비스 구조를 확장하여 이벤트 모니터, 실시간 스케줄러, 실시간 이벤트 서비스 등의 객체 컴포넌트를 포함하는 구조를 가진다. 특히, 본 논문에서는 전자상거래 사용자의 실시간 요구사항을 지원할 수 있는 실시간 이벤트 필터링을 제공하는 것에 중점을 두었다. 사용자로부터 시간적 제약조건과 원하는 이벤트 정보를 입력받아 QoS저장소에 저장하고, 실시간 이벤트가 발생하며RTFA(Real-Time Fitering Agent)를 통해 소비자가 원하는 이벤트를 필터링하여 제공하게 된다. 이로써 사용자는 보다 높은 서비스를 제공받게 되고 이 과정을 통해 전체적인 네트워크 트래픽이 감소되는 효과를 얻을 수 있다.

• Structural Engineering and Mechanics
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• 제25권4호
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• pp.481-500
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• 2007

The dynamic instability of doubly curved panels, subjected to non-uniform tensile in-plane harmonic edge loading $P(t)=P_s+P_d\;{\cos}{\Omega}t$ is investigated. The present work deals with the problem of the occurrence of combination resonances in contrast to simple resonances in parametrically excited doubly curved panels. Analytical expressions for the instability regions are obtained at ${\Omega}={\omega}_m+{\omega}_n$, (${\Omega}$ is the excitation frequency and ${\omega}_m$ and ${\omega}_n$ are the natural frequencies of the system) by using the method of multiple scales. It is shown that, besides the principal instability region at ${\Omega}=2{\omega}_1$, where ${\omega}_1$ is the fundamental frequency, other cases of ${\Omega}={\omega}_m+{\omega}_n$, related to other modes, can be of major importance and yield a significantly enlarged instability region. The effects of edge loading, curvature, damping and the static load factor on dynamic instability behavior of simply supported doubly curved panels are studied. The results show that under localized edge loading, combination resonance zones are as important as simple resonance zones. The effects of damping show that there is a finite critical value of the dynamic load factor for each instability region below which the curved panels cannot become dynamically unstable. This example of simultaneous excitation of two modes, each oscillating steadily at its own natural frequency, may be of considerable interest in vibration testing of actual structures.