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RPK118, a PX Domain-containing Protein, Interacts with Peroxiredoxin-3 through Pseudo-Kinase Domains  

Liu, Lungling (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Yang, Chenyi (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Yuan, Jian (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Chen, Xiujuan (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Xu, Jianing (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Wei, Youheng (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Yang, Jingchun (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Lin, Gang (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Yu, Long (State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University)
Publication Information
Molecules and Cells / v.19, no.1, 2005 , pp. 39-45 More about this Journal
Abstract
RPK118 is a sphingosine kinase-1-binding protein that has been implicated in sphingosine 1 phosphate-mediated signaling. It contains a PX (phox homology) domain and two pseudo-kinase domains, and co-localizes with sphingosine kinase-1 on early endosomes. In this study we identified a novel RPK118-binding protein, PRDX3 (peroxiredoxin-3), by yeast two-hybrid screening. The interaction between these proteins was confirmed by pull-down assays and co-immunoprecipitation experiments. Deletion studies showed that RPK118 interacted with PRDX3 through its pseudokinase domains, and with early endosomes through its PX domain. Double immunofluorescence experiments demonstrated that PRDX3 co-localized with RPK118 on early endosomes in COS7 cells. PRDX3 is a member of the antioxidant family of proteins synthesized in the cytoplasm and functioning in mitochondria. Our findings indicate that RPK118 is a PRDX3-binding protein that may be involved in transporting PRDX3 from the cytoplasm to its mitochondrial site of function or to other membrane structures via endosome trafficking.
Keywords
Co-immunoprecipitation; Co-localization; Early Endosome; PRDX3; RPK118; Yeast Two-Hybrid;
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