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The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes

  • Tsuzuki, Y.;Nozawa, K.;Ashizawa, K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.3
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    • pp.328-335
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    • 2009
  • This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs) immature porcine oocytes. The in vitro nuclear maturation of the COs, exposed to and removed from the CPAs was also assessed. Both COs and DOs were exposed to 1.5 M concentrations of each CPA (ethylene glycol (EG); propylene glycol (PG); dimethyl-sulfoxide (DMSO); and glycerol (G)) for durations of 5, 15, and 30 minutes at room temperature ($23.5{\pm}1.5^{\circ}C$), and immersed in physiological saline supplemented with 20% (v/v) fetal bovine serum for 5 minutes ($39^{\circ}C$) to remove each CPA. Before, during and after exposure to each CPA, the ATP content of both the COs and the DOs was measured. After removal from each CPA an aliquot of the COs was matured for 44${\pm}$2 h, and their nuclear maturation rates were measured up to the metaphase stage of the second meiotic division (the M-II stage). The maturation rates up to the M-II stage were not significantly different between all the groups that were exposed to each CPA for 5 minutes. For 15 and 30 minute exposures, the maturation rates of the COs exposed to PG, DMSO and EG were almost the same as those of the control groups; however, the rates of G group exposed for 15 and 30 minutes were significantly lower (p<0.05) than the control group. These groups were also found to have a decrease in the ATP content of COs and DOs during and after exposure for the same periods (p<0.05). In addition, although the ATP contents of the COs after exposure to EG for any period were the same as the controls, the ATP content of the DOs exposed to EG for any period were significantly lower (p<0.05) than those of the controls. When the ATP content of the COs and DOs of each CPA were compared, the DOs exposed to PG were found to have a significantly greater level (p<0.05) than DOs exposed to G for any duration. In addition, the ATP content of DOs exposed to PG for 30 min and removal was also higher (p<0.05) than when exposed to DMSO for the same period. These findings indicate that PG may be a useful CPA for the cryopreservation of immature porcine oocytes.

Effect of Chito-oligosaccharide Supplementation on Immunity in Broiler Chickens

  • Deng, Xingzhao;Li, Xiaojing;Liu, Pai;Yuan, Shulin;Zang, Jianjun;Li, Songyu;Piao, Xiangshu
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.11
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    • pp.1651-1658
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    • 2008
  • This study was conducted to determine the effects of dietary supplementation of either 100 mg/kg chito-oligosaccharide (COS) or chlortetracycline (CTC) with corn-soybean-fish meal on immunity in broiler chickens. A total of 147 one-day old male broiler chicks were randomly allocated to 3 treatments with 7 replicate pens per treatment and 7 birds per pen. The experimental diets consisted of a control diet based on corn, soybean and fish meal without COS and any antibiotic supplement and similar diets supplemented with either CTC (80 mg/kg from d 1 to 21 and 50 mg/kg from d 22 to 42) or COS (100 mg/kg from d 1 to 42). During the entire experimental period, all birds had ad libitum access to diets and water. The main immune organ indices, T-lymphocyte proliferation, serum cytokine concentrations, serum NO level and serum iNOS activity were measured on d 21 and d 42. On d 21, broilers fed 100 mg/kg COS had improved (p<0.01) indices of spleen, thymus, and bursa of Fabricius compared with the control and CTC birds. Birds receiving 100 mg/kg COS had higher (p<0.05) serum concentrations of $IL-1{\beta}$, IL-6, IgM, NO and iNOS than birds on the control treatment. Serum $Ca^{2+}$ level of birds fed 100 mg/kg COS tended to be higher (p = 0.049) than in birds fed CTC. On d 42, the birds fed 100 mg/kg COS had higher (p<0.05) concentrations of TNF-${\alpha}$ and IgM in serum than birds in both the CTC and control treatments. Birds fed 100 mg/kg COS had a higher concentration of IFN-$\gamma$ than the control group. In conclusion, dietary supplementation of COS appeared to improve the immunity of broilers by promoting the weight of the main immune organs, increasing IgM secretion, stimulating microphages to release $TNF-{\alpha}$, $IL-1{\beta}$, IL-6 and IFN-$\gamma$, and activating iNOS to induce NO.

Enhancement of Chitooligosaccharides in Doenjang (Soybean Paste) and Kanjang (Soy Sauce) using Bacillus subtilis Koji and Rhizopus oryzae Koji (Bacillus subtilis koji와 Rhizopus oryzae koji를 이용한 된장 및 간장의 키토올리고당 함량 증대)

  • Eum, Byong-Wook;Kwak, Bo-Yeon;Kim, Soon-Young;Shon, Dong-Hwa;Lee, Ke-Ho
    • Korean Journal of Food Science and Technology
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    • v.35 no.2
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    • pp.291-296
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    • 2003
  • Effects of Bacillus subtilis and Rhizopus oryzae on chitooligosaccharides (COS) content of doenjang (soybean paste) and kanjang (soy sauce) were investigated using kojis made with the two strains. Competitive direct enzyme-linked immunosorbent assay (cdELISA) system using anti-COS mixture (COSM) antibody was applied for COS detection ranging from 0.001 to $1{\mu}g/mL$, and the recoveries of COSM spiked to doenjang and kanjang were 102 and 115%, respectively. Doenjang and kanjang products made with a mixture of B. subtilis and R. oryzae kojis showed COS contents of 171 and $29{\mu}g/mL$, respectively, during two-month aging period, much higher than those of Japanese and Korean commercial ones.

New Serial and Parallel Sin+Cos PSS1A PSS Design and Analysis

  • Lee Sang-Seung;Li Shan-Ying;Jang Gwang-Soo;Park Jong-Keun;Moon Seung-Il;Yoon Yong-Tae
    • KIEE International Transactions on Power Engineering
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    • v.5A no.4
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    • pp.378-384
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    • 2005
  • This paper proposes a new series and parallel Sin+Cos PSS (power system stabilizer) for the purpose of improving the existing PSS1A's performance. The purpose of the PSS is to enhance the damping of power system oscillations through injection of auxiliary signals for an excitation control terminal. The proposed series and parallel Sin+Cos PSS is connected adding the Sin+Cos terms additionally with the serial and parallel connection in a conventional PSS1A. The proposed controller is aimed at considering the damping of oscillation when it changes parameter fluctuations or operational load variations in a power system. The electric power system used is the KEPCO system and the voltage of the power transmission line is 154kV and 345kV. The PSCAD/EMTDC package is used to authorize the effect of the proposed controller. Simulations were shown by and compared with the waveforms for frequency, voltage and electric power.

Periodic Variations of Water Temperature in the Seas Around Korea(I) Annual and Secular Variations of Surface Water Temperature, Kumun-Do Region, Southern Sea of Korea (한국 근해 수온의 주기적 변화(I) 남해의 거문도해역 표면수온 년주변화 및 영년변화)

  • Hahn, Sangbok
    • 한국해양학회지
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    • v.5 no.1
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    • pp.6-13
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    • 1970
  • Ten days and monthly mean temperatures were analysed daily data observed during July, 1916 to March, 1970 statistically. Periodic characters were calculated by Δn, new method of approximate solution of Schuster Method. According to ten days mean temperatures, annual variation function is F($\theta_d$)=16.29-5.27 cos $\theta_d$+0.75 cos2 $\theta_d$-3.14 sin $\theta_d$+1.16 sin2 $\theta_d$-0.63 sin $\3{theta}_d$, where $\theta_d$=$-\frac{\pi}{18}$(d-3), d is the order of ten days period, 1 to 36. Annual mean water temperature is 16.3$^{\circ}C$, minimum in the last ten days of February 10.9$^{\circ}C$, maximum in the last ten days of August 24.5$^{\circ}C$. Periodic character of secular variation shows 11 year and its curve is F($\theta_y$)=16.29+0.53 cos $\theta_y$ -0.16cos $2{\theta}_y$+0.10 cos$3{\theta}_y$-0.10 sin $\theta_y$, where $\theta_y$=2$-\frac{2\pi}{11}$(y-1920), y is calendar year. And the relation between air temperature x and water temprature y is following. y=9.67 1.035$\^x$

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Security Analysis of SCOS-3 Block Cipher against a Related-Key Attack (블록 암호 SCOS-3의 연관키 차분 공격에 대한 안전성 분석)

  • Lee, Chang-Hoon
    • Journal of Advanced Navigation Technology
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    • v.13 no.6
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    • pp.977-983
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    • 2009
  • Recently, several DDP, DDO and COS-based block ciphers have been proposed for hardware implementations with low cost. However, most of them are vulnerable to related-keyt attacks. A 12-round block cipher SCOS-3 is desinged to eliminate the weakness of DDP, DDO and COS-based block ciphers. In this paper, we propose a related-key differential attack on an 11-round reduced SCOS-3. The attack on an 11-round reduced SCOS-3 requires $2^{58}$ related-key chosen plaintexts and $2^{117.54}$ 11-round reduced SCOS-3 encryptions. This work is the first known attack on SCOS-3. Therefore, SCOS-3 is still vulnerable to related-key attacks.

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Reactive Silencer Design for Suppressing Impulse Noise from a High Voltage Cut Out Switch Fuse (고전압 COS 퓨즈로부터 방사된 충격성 소음 저감용 반사형 소음기의 설계)

  • Song, Hwa-Young;Kim, Deok-Han;Lee, Jong-Suk;Lee, Dong-Hoon
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.05a
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    • pp.951-954
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    • 2006
  • This Study introduces the design of a reactive type silencer for reducing impulse noises emitted from a high voltage COS fuse of a transformer. When a high voltage COS fuse becomes a short circuit by the over current, the peak sound pressure level above 150dB(A) is generated at the distance of 2m from a COS Fuse. For the purpose of the reduction of impulse noise, in this study. the reactive type silencers have been utilized. The performance of noise reduction for them can be expressed by insertion loss. The reactive silencers have been tested for 23 different types with each different porosity, hole diameter and depth. From the experimental results, it is found that the reactive silencer has an excellent performance to greatly suppress the impulse noise. The one-stage, two-stage and three-stage reactive type silencers have been shown to have the insertion losses of about from 35dB (A) to 25dB(A).

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Approximation of Polynomials and Step function for cosine modulated Gaussian Function in Neural Network Architecture (뉴로 네트워크에서 코사인 모듈화 된 가우스함수의 다항식과 계단함수의 근사)

  • Lee, Sang-Wha
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.49 no.2
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    • pp.115-122
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    • 2012
  • We present here a new class of activation functions for neural networks, which herein will be called CosGauss function. This function is a cosine-modulated gaussian function. In contrast to the sigmoidal-, hyperbolic tangent- and gaussian activation functions, more ridges can be obtained by the CosGauss function. It will be proven that this function can be used to aproximate polynomials and step functions. The CosGauss function was tested with a Cascade-Correlation-Network of the multilayer structure on the Tic-Tac-Toe game and iris plants problems, and results are compared with those obtained with other activation functions.

A Deadline_driven CPU Power Consumption Management Scheme of the TMO-eCos Real-Time Embedded OS (실시간 임베디드 운영체제 TMO-eCos의 데드라인 기반 CPU 소비 전력 관리)

  • Park, Jeong-Hwa;Kim, Jung-Guk
    • Journal of KIISE:Computing Practices and Letters
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    • v.15 no.4
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    • pp.304-308
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    • 2009
  • This paper presents the deadline driven CPU-Power management scheme for the Real-Time Embedded OS: named TMO-eCos. It used the scheduling scenarios generated by a task serialization technique for hard real- time TMO system. The serializer does a off-line analysis at design time with period, deadline and WCET of periodic tasks. Finally, TMO-eCos kernel controls the CPU speed to save the power consumption under the condition that periodic tasks do not violate deadlines. As a result, the system shows a reasonable amount of power saving. This paper presents all of these processes and test results.