• Journal of Microbiology
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• v.44 no.5
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• pp.530-536
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• 2006

Bacteriophage P4, a satellite phage of coliphage P2, is a very useful experimental tool for the study of viral capsid assembly and cos-cleavage. For an in vitro cos-cleavage reaction study of the P2-P4 system, new shortened and selectable markers containing P4 derivative plasm ids were designed as a substrate molecules. They were constructed by swapping the non-essential segment of P4 DNA for either the kanamycin resistance (kmr) gene or the ampicillin resistance (apr) gene. The size of the genomes of the resulting markers were 82% (P4 ash8 delRI:: kmr) and 79% (P4 ash8 delRI:: apr) of the wild type P4 genome. To determine the lower limit of genome size that could be packaged into the small P4-size bead, these shortened P4 plasmids were converted to phage particles with infection of the helper phage P2. The conversion of plasmid P4 derivatives to bacteriophage particles was verified by the heat stability test and the burst size determination experiment. CsCl buoyant equilibrium density gradient experiments confirmed not only the genome size of the viable phage form of shortened P4 derivatives, but also their packaging into the small P4-size head. P4 ash8 delRI:: apr turned out to be the smallest P4 genome that can be packaged into P4-sized head.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.277-281
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• 2004

The following paper proposes the new design of digital sine wave generator which allows users to define the phase shift of the out put sinewave according to user's demands. This new sinewave generator will have 2 outputs, cos(${\omega}_0n$) and cos(${\omega}_0n$+${\phi}$) The design of the new system starts from the construction of discrete time system with impulse response as cos(${\omega}_0n$) in a pair of conjugate complex poles and a pair of zeros at the origin and the real axis. If users want to make a phase shift of sign wave, users can change the position of zero at the real axis. The results of the experiment have shown that the new design of sign wave generator has generated sine wave with the correct phase shift according to the theory.

• Applied Microscopy
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• v.43 no.3
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• pp.117-121
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• 2013

Mitochondrion is an important intracellular organelle controlling energy production essential for cell survival. In addition, it is closely related to cellular apoptosis and necrosis. Linear, branched, circular, and ball-shaped mitochondria have been reported. Recent research suggests that mitochondrial morphology may reflect functional status of the cell. In this study, we investigated the density and ratio of the each morphological categories of mitochondria in a few normal cultured cells; astrocyte, HeLa and COS7 cells, of which metabolic activities are different, with high voltage electron microscopy. The absolute number and relative number per unit area of mitochondria was largest in astrocyte. But, the proportion of different mitochondrial shape was similar among cells. These results shows the numerical profiles but not morphological profiles of mitochondria are related to the metabolic activity of each cell line.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.28 no.3
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• pp.18-25
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• 2014

In this paper, tried to find out the proper luminous intensity distribution for the cabin with low-level height by analyzing the changing tendency of discomfort glare according to luminous intensity distribution of marine indoor luminaires using Unified Glare Rating(UGR). First, we analyzed UGR of the indoor luminaires in the existing cabin, and then evaluated influence on luminous intensity distributions of marine indoor luminaire. The results showed that $cos^2{\Theta}$ distribution got almost low UGR results regardless of height and UGR 16.5 in cabin height of 2m. However, Gaussian distribution with the same beam angle showed that UGR results consistently increased by getting lower height and UGR 20.7 in the same height. In conclusion, the $cos^2{\Theta}$ distribution in consideration of luminous intensity on the direction of observer's eye was appropriate for general cabin indoor luminaires because it decreased UGR in the low-level height.

• 한국신재생에너지학회:학술대회논문집
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• 2011.11a
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• pp.177.2-177.2
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• 2011

폐기물 합성가스에 포함되어 있는 오염물질 중 황화합물($H_2S$, COS)이 존재할 경우, 다른 연계 공정을 구성하는 설비의 부식, 합성가스 이용 공정의 촉매 피독 문제, 대기 배출시 환경오염 문제 등을 야기 시키므로 제거가 필요하다. 고온 정제 공정을 적용하여 황화합물을 제거하기위해 산화아연을 이용한 고온 탈황 실험을 수행하였다. 고정층 반응기에 탈황제로 선정한 산화아연을 충진하고, 공간속도 $3,000h^{-1}$, 입구 황화합물의 농도 $H_2S$ 1,000ppm, COS 300ppm일 때 반응 온도 변화에 따른 탈황특성을 살펴보았다. 가스 내부에 $H_2S$가 단독으로 존재할 경우에는 $400^{\circ}C$ 이상에서 모두 제거되었으나, $H_2S$와 COS가 동시에 존재할 경우에는 $450^{\circ}C$ 이상에서 모두 제거되는 것을 알 수 있었다. 반응온도 $500^{\circ}C$에서 산화아연 탈황제를 이용한 실험결과 $H_2S$, COS의 파과시간은 각각 1,217, 1,063 min, 흡착능력은 269.9 mg-$H_2S$/g-sorbent, 124.7 mg-COS/g-sorbent으로 파악되었다.

• Korean Journal of Microbiology
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• v.39 no.4
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• pp.277-282
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• 2003

Bacteriophage P4 ost2 which is the P4 mutant suppressing sir mutations of Bacteriophage P2, was isolated as a plaque-former by plating P4 ash8 sid 71 kmr intS on the lawn of P2 sir3 lysogen. P4 ost2 turned out to be the P4 mutant suppressing sir mutations of P2 in one-step growth experiments.

• Journal of the Korean Society of Safety
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• v.26 no.1
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• pp.8-14
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• 2011

Noise and vibration are likely to disturb the sensory system of human body leading to psychological stress and thereby property damage. In this research, a cut out switch(COS) with a built-in acoustic absorber along with a COS fuse broken was developed to reduce percussion noise. This new system is based on a design approach that combines existing absorber systems: expansion type, resonator type, and acoustic absorber type silencer The noise performance of the new system was simulated using the $SYSNOISE^{TM}$ software under optimized parameters: the diameter of perforated plate 2 mm, the plate thickness 3 mm, the width of expansion room 25 mm, the impinging vortex room 14 mm, and the noise absorbtion room 10 mm. The results showed that it reduced noise by approximately 41.1 dB compared to the current systems available in the market. Furthermore, it showed reduced noise by approximately 12 dB more than a product with an acoustic absorber of the Fault Tamer(USA).

• Journal of the Korea Society of Computer and Information
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• v.11 no.3
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• pp.107-115
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• 2006

This paper presents a new class of activation functions for Cascade Correlation learning algorithm, which herein will be called CosGauss function. This function is a cosine modulated gaussian function. In contrast to the sigmoidal, hyperbolic tangent and gaussian functions, more ridges can be obtained by the CosGauss function. Because of the ridges, it is quickly convergent and improves a pattern recognition speed. Consequently it will be able to improve a learning capability. This function was tested with a Cascade Correlation Network on the two spirals problem and results are compared with those obtained with other activation functions.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.96-96
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• 2002

We examined the role of SR 144528 (N-[-(1S-endo-1,3,,3-trimethyl-bicycle[2, 2, 1 ] heptan-2-y1]-5-(-4-chloro-3-mothyl-phenyl)-(4-methylbenzyl)-pyrazole-3- carboxamide) in the modulation of certain AC isoforms in transiently transfected COS-7 cells. We found that CB2 in COS cells has a constitutive activity, and thus leading to inhibition of AC-V activity even in the absence of agonist. In addition, this constitutive modulation of AC is reversed by SR144528. It is now well established that several G protein-coupled receptors can signal without agonist stimulation(constitutive receptors). Inverse agonists have been shown to inhibit the activity of such constitutive G protein-coupled receptor signaling. Agonist activation of the G$\_$i/o/-coupled peripheral cannabinoid receptor CB2 normally inhibits adenylyl cyclase type V and stimulates adenylyl cyclase type II. Using transfected COS cells, we show here that application of SR144528, an inverse agonist of CB2, leads to a reverse action (stimulation of adenylyl cyclase V and inhibition of adenylyl cyclase II). This inverse agonism of SR144528 is dependent on the temperature, as well as on the concentration of the cDNA of CB2 transfected. Pertussis toxin blocked the regulation of adenylyl cyclase activity by SR 144528.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.723-731
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• 2008

Immunomodulatory feed additives might offer alternatives to antimicrobial growth promoters in pig production. This experiment was designed to determine the effects of dietary galacto-mannan-oligosaccharide (GMOS) and chitosan oligosaccharide (COS) supplementation on the immune response in early-weaned piglets. Forty 15-day-old piglets (Duroc$\times$Landrace$\times$Yorkshire) with an average live body weight of $5.6{\pm}0.51kg$ were weaned and randomly assigned to 4 treatment groups that were fed maize-soybean meal diets containing either basal, 110 mg/kg of lincomycin, 250 mg/kg of COS or 0.2% GMOS, respectively, over a 2-week period. Another six piglets of the same age were sacrificed on the same day at the beginning of the study for sampling, in order to obtain baseline values. Interleukin (IL)-1${\beta}$gene expression in peripheral blood monocytes, jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2 and IL-6, IgA, IgG, and IgM, were evaluated for 5 pigs from each group at 15 and 28 days of age. The results indicate that weaning stress resulted in decreases in serum antibody and cytokine levels. Dietary supplementation with GMOS or COS enhanced (p<0.05) IL-1${\beta}$gene expression in jejunal mucosa and lymph nodes, as well as serum levels of IL-1${\beta}$ IL-2, IL-6, IgA, IgG and IgM compared to supplementation with lincomycin. These findings suggest that GMOS or COS may enhance the cell-mediated immune response in early-weaned piglets by modulating the production of cytokines and antibodies, which shows that GMOS or COS have different effects than the antibiotic on animal growth and health.