• Title/Summary/Keyword: correlated parameters

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Phylogenetic characterization of bacterial populations in different layers of oak forest soil (상수리나무림의 토양 층위별 세균군집의 계통학적 특성)

  • Han, Song-Ih
    • Korean Journal of Microbiology
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    • v.51 no.2
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    • pp.133-140
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    • 2015
  • We have examined the correlation between the physicochemical and microbiological environment variables for the different layers of oak forest soil in Mt. Gyeryong, Korea. The result shows that there is a high correlation in the environment variables between the soil parameters of the fermented (F) layer and humus (H) layer. In particular, the pH level in the F layer shows a high correlation with C and N, while the various organic acids of the H layer turns out to be closely correlated with soil bacteria density. As we evaluated phylogenetic characteristics of bacterial populations by DGGE analysis with DNA extracted. Total of 175 bands including 43 bands from litter (L) layer, 42 bands from F layer, 43 bands from H layer and 47 bands from rhizosphere (A) layer were selected as the major DGGE band of oak forest soil. Based on the 16S rRNA gene sequences, 175 DGGE bands were classified into 32 orders in 7 phylum. The heat map was analyzed in order to compare the quantity of the base sequences of each order and based on the clustering of the different layers of oak forest soil, the result confirms that the F layer and H layer belong to a different cluster from that of L layer and A layer. Furthermore, it also showed that approximately 50% of the total microbial population in different layers is ${\alpha}$-proteobacteria, which indicates that they belong to the dominant system group. In particular, Rhizobiales, Burkholderiales and Actinobacteriales were observed in all the seasons and layers of oak forest soil, which confirms that they are the indigenous soil bacterial community in oak forest soil.

Effect of temperature on single- and mixed-strain fermentation of ruminant feeds

  • Woo, Seungmin;Kim, Sooah;Ye, Suji;Kim, Soo Rin;Seol, Jeongman;Dooyum, Uyeh Daniel;Kim, Junhee;Hong, Dong Hyuck;Kim, Jong Nam;Ha, Yushin
    • Journal of Animal Science and Technology
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    • v.62 no.2
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    • pp.227-238
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    • 2020
  • Use of raw feedstuffs for livestock is limited by low digestibility. Recently, fermentation of feedstuffs has been highlighted as a new way to improve nutrient absorption through the production of organic acids using inoculated microorganisms, which can also play a probiotic role. However, standard procedures for feedstuff fermentation have not been clearly defined because the process is influenced by climatic variation, and an analytical standard for fermented feedstuffs is lacking. This study aimed to evaluate the microbiological and biochemical changes of feedstuffs during fermentation at temperatures corresponding to different seasons (10℃, 20℃, 30℃, and 40℃). We also investigated the effects of yeast, lactic acid bacteria (LAB), and Bacillus spp. on fermentation and determined the results of their interactions during fermentation. The viable cells were observed within 8 days in single-strain fermentation. However, when feedstuffs were inoculated with a culture of mixed strains, LAB were predominant at low temperatures (10℃ and 20℃), while Bacillus spp. was predominant at high temperatures (30℃ and 40℃). A significant drop in pH from 6.5 to 4.3 was observed when LAB was the dominant strain in the culture, which correlated with the concentrations of lactic acid. Slight ethanol production was detected above 20℃ regardless of the incubation temperature, suggesting active metabolism of yeast, despite this organism making up a marginal portion of the microbes in the mixed culture. These results suggested that fermentation temperature significantly affects microbiological profiles and biochemical parameters, such as pH and the lactic acid concentration, of fermented feedstuffs. Our data provide valuable information for the determination of industrial standards for fermented feedstuffs.

Statistical Characteristics of the Non-tidal Components Data in Korean Coasts (한반도 연안 비조석 성분자료의 통계적 특성)

  • Cho, Hong-Yeon;Jeong, Shin-Taek;Yoon, Jong-Tae;Kim, Chang-Il
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.18 no.2
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    • pp.112-123
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    • 2006
  • Double-peak normal distribution function was suggested as the probability density function of the non-tidal components (NTC) data in Korean coastal zone. Frequency distribution analysis of the NTC data was carried out using hourly tidal elevation data of the ten tidal gauging stations, i.e., Incheon, Gunsan, Mokpo, Jeju, Yeosu, Masan, Gadeokdo, Busan, Pohang, and Sokcho which were served through the Internet Homepage by the National Ocean Research Institute. NTC data is defined as the difference between the measured tidal elevation data and the astronomical tidal elevation data using 64 tidal constituents information. Based on the RMS error and R2 value comparison analysis, it was found that this suggested function as the probability density function of the NTC data was found to be more appropriate than the normal distribution function. The parameters of the double-peak function were estimated optimally using Levenberg-Marquardt method which was modified from the Newton method. The standard deviation and skewness coefficient were highly correlated with the non-tidal constants of the tidal gauging stations except Mokpo, Jeju and Sokcho stations.

Growth Characteristics of Blue-green Algae (Anabaena spiroides) Causing Tastes and Odors in the North-Han River, Korea (북한강 수계에서 이취미를 유발하는 남조류(Anabaena spiroides)의 증식 특성)

  • You, Kyung-A;Byeon, Myeong-Seop;Youn, Seok-Jea;Hwang, Soon-Jin;Rhew, Doug-Hee
    • Korean Journal of Ecology and Environment
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    • v.46 no.1
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    • pp.135-144
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    • 2013
  • Blue-green algae blooms occurred during early winter in the North-Han River, Korea. Among blue-green algae, Anabaena spiroides were observed for approximately 33 consecutive days, between 28 November and 30 December, 2011. A. spiroides emerged from Lake Uiam to Lake Paldang, depending on the flow of the river has spread downstream. Changes of physical water environment like rising water temperature and increasing hydraulic retention time influenced the A. spiroides bloom. The A. spiroides bloom showed a very rapid increase in cell density, and a slow decrease: the cell density increased to a maximum of $11,325cells\;mL^{-1}$ in Lake Paldang (st. 5), and was completely disappeared after the water temperature dropped below $4^{\circ}C$. A decrease in water temperature was the most influential factor among all environmental parameters, for the reduction of A. spiroides cell density. The A. spiroides bloom was accompanied with the occurrence of very high concentrations of the odor metabolite geosmin. Geosmin reached the peak value of $1,640ng\;L^{-1}$ in Lake Paldang (st. 4). The geosmin concentration was very strongly correlated with cell numbers of A. spiroides.

Characterization of Korean Sweet Potato Starches: Physicochemical, Pasting, and Digestion Properties (국내 육종 고구마 전분의 이화학 호화 및 소화 특성)

  • Baek, Hye Rim;Kim, Ha Ram;Kim, Kyung Mi;Kim, Jin Sook;Han, Gui Jung;Moon, Tae Wha
    • Korean Journal of Food Science and Technology
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    • v.46 no.2
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    • pp.135-142
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    • 2014
  • Physicochemical, pasting, and digestion properties of sweet potato starches from 11 Korean cultivars were investigated. Starch granules were variably oval, round, polygonal, spherical, and bell-shaped, and of 10.2-15.3 ${\mu}m$ in mean particle diameter. Amylose contents varied from 12.3 to 17.4%. A similar chain length distribution of amylopectin was found in each of the cultivars. The portion of $B_3$ correlated with the degree of amylose leaching. Thermal properties determined by differential scanning calorimetry showed high values of gelatinization temperatures in Shinyulmi and Jeungmi starches, but a relatively low value in Daeyumi starch. All starches exhibited a Ca-type diffraction pattern. Differing patterns were observed in swelling factors, depending on temperature. The contents of rapidly digestible starch, slowly digestible starch, and resistant starch ranged from 9.6-17.4, 31.4-45.6, and 35.7-62.8%, respectively. In Rapid Visco Analyser profiles, differences were observed in pasting parameters such as pasting temperature, peak viscosity, final viscosity, and breakdown.

Phytoplankton and Environmental Factors in Lake Hwaong (화옹호의 식물플랑크톤과 환경요인)

  • Chung, Mi-Hee;Kim, Ho-Sub;Choi, Chung-Il;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.37 no.2 s.107
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    • pp.193-204
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    • 2004
  • This study was conducted to evaluate both temporal and spatial dynamics of phytoplankton community and environmental parameters in a newly made reclaimed esturine lake (L. Hwaong). Monthly sampling was conducted at 4 sites covering the longest transect of longitudinal gradient of the lake from June to November, 2002. Total 5 classes 8 orders 26 families 83 genus 192 species were identified at all study sites during the study period. Phytoplankton total cell density ranged 24${\sim}$ 1,882 cells $mL^{-1}$ and highly varied both temporally and spatially. Total cell density was significantly related with salinity, pH, BOD, COD, SS, TN and TP concentration. Diatom density also was significantly correlated with salinity, SS, BOD, COD and TN concentration, Although there was spatial difference, a longtudinal gradient appeared in phytoplankton cell density, Chl-a, TN and TP concentration from the mouth of river in June and August. In conclusion, phytoplakton community structure was dominated by diatoms (Bacillariophyceae), and appeared to be largely influenced by salinity, precipitation, and nutrients during the summer and the fall.

Genetic parameter estimation for reproductive traits in QingYu pigs and comparison of carcass and meat quality traits to Berkshire×QingYu crossbred pigs

  • Luo, Jia;Yang, Yiting;Liao, Kun;Liu, Bin;Chen, Ying;Shen, Linyuan;Chen, Lei;Jiang, An'an;Liu, Yihui;Li, Qiang;Wang, Jinyong;Li, Xuewei;Zhang, Shunhua;Zhu, Li
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.8
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    • pp.1224-1232
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    • 2020
  • Objective: The QingYu pig is well known for its excellent meat quality attributes in Sichuan province, China. In order to improve its production efficiency, the determination of genetic factors contributing to quantifiable economic traits of livestock is important. Moreover, the cross-breeding of QingYu pigs with western breeds possessing strong growth attributes is an efficient way to improve the performance of this breed. Methods: Here, the genetic parameters of several important reproductive traits of QingYu pigs were estimated, include total number born (TNB), number born alive, litter birth weight, individual birth weight, number of piglets weaned, litter weaning weight, and individual weaning weight. The data was analyzed using the ASReml 3.0 software (NSW Inc., Sydney, Australia). Furthermore, the effects of crossing Berkshire with QingYu (BQ) pigs on carcass and meat quality traits, as well as the effects of slaughter weight on carcass and meat quality of BQ were characterized. Results: QingYu pigs exhibited superior reproductive traits. The TNB available to QingYu pigs was more than 8 per parity. The observed repeatability of the reproductive traits of the QingYu pigs was between 0.10 and 0.23. The significantly correlated genetic and phenotypic of reproduction traits were consistent. Interestingly, the BQ pigs exhibited improved carcass quality, with a significant increase in loin muscle area, lean percentage and reduction in sebum percentage. As a result, BQ had higher L45min, lower cooking scores, and lower drip loss. In addition, the loin muscle area, body length, and sebum percentage were significantly higher in 90 and 100 kg animals. Cooking loss showed a significant increase at 80 kg, and marbling increased significantly from 90 kg. Conclusion: The results of this study suggest that QingYu pigs exhibit excellent reproductive properties and heritability of these traits. Crossing with Berkshire is an efficient strategy to improve the carcass and meat quality of QingYu pigs for commercial operations. Furthermore, it appears as though the optimal slaughter weight of BQ pigs is at approximately 90 kg.

Regional Distribution of Hydrocarbon Degrading Bacteria in the Sediment of South Sea, Korea (남해해역 퇴적토의 탄화수소 분해세균 분포)

  • 김상진;최성찬
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.366-370
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    • 1992
  • Sediment samples were collected from the stations 0101-0921 located between N $32^{\circ}$30'~$34^{\circ}$30' and E $123^{\circ}$30'-$128^{\circ}$30' during July 31-August lO. 1988. The distributions of total heterotrophic bacteria, freshwater bacteria and hydrocarbon degrading bacteria were studied. Each bacterial distribution was in the range of $3{\times}10^{5}~9.2{\times}10^{6}CFU/cm^{3}$sediment, $3{\times}10^{3}~2.1{\times}10^{6}CFU/cm^{3}$ sediment and $2{\times}10^{4}~6.2{\times}10^{6}CFU/cm^{3}$ sediment. respectively. The percent of hydrocarbon degrading bacteria against total heterotrophic bacteria was 0.7-73,2 % which was much higher than other marine sediments reported. These values were statistically analyzed with the percent of freshwater bacteria against total heterotrophic bacteria. These two parameters were well correlated with the correlation coefficient r= 0.60058 (n=34) and P=0.OOO2. This means that the distributions of hydrocarbon degrading bacteria and freshwater bacteria in the research area were affected together by the fresh water discharge into the sea environment. Therefore it can be concluded that the distribution of hydrocarbon degrading bacteria in the sediment of South Sea was affected by petroleum hydrocarbon input from terrestrial region through rivers.

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Studies of Solvolyses of Biphenyl-4-carbonyl Chloride by Extended Grunwald-Winstein Equation (확장된 Gruwald-Winstein 식에 의한 Biphenyl-4-carbonyl chloride의 가용매분해 반응대한 연구)

  • Choi, Hojune;Koh, HanJoong;Yang, Kiyull;Koo, InSun
    • Journal of the Korean Chemical Society
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    • v.60 no.1
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    • pp.16-20
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    • 2016
  • The solvolysis rate constants of biphenyl-4-carbonyl chloride (C6H5C6H5COCl, 1) in 19 different solvents are well correlated with the extended Grunwald-Winstein equation, using the NT solvent nucleophilicity scale, YCl solvent ionizing scale, and I aromatic ring parameter with sensitivity values of 0.31±0.10, 0.46±0.05, and 0.96±0.20 for l, m, and h, respectively. These l, m, and h values can be considered to support a dissciative SN2 reaction pathway. This interpretation is further supported by the activation parameters, i.e., relatively small positive ΔH (15.3~16.1 kcal/mol) values and large negative ΔS (−17.2~−20.0 cal/mol·K) values.

Effects of Flow Direction and Consolidation Pressure on Hydraulic Resistance Capacity of Soils (흐름방향과 압밀응력이 지반의 수리저항특성에 미치는 영향)

  • Kim, Youngsang;Jeong, Shinhyun;Lee, Changho
    • Journal of the Korean GEO-environmental Society
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    • v.16 no.5
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    • pp.55-66
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    • 2015
  • Big tidal differences, which range from 3.0 m to 8.0 m, exist with regional locations at south and west shores of Korea. Under this ocean circumstance, since a large scour may occur due to multi-directional tidal current and transverse stress of the wind, the scour surrounding the wind turbine structure can make instability of the system due to unexpected system vibration. The hydraulic resistance capacity of soils consolidated under different pressures are evaluated by Erosion Function Apparatus (EFA) under unidirectional and bi-directional flows in this study. It was found that the flow direction change affects significantly on the sour rate and critical shear stress, regardless of soil types while the consolidation pressure affects mainly cohesive soil. Among geotechnical parameters, the undrained shear strength can be well-correlated with the hydraulic resistance capacity, regardless soil type while the shear wave velocity shows the proportional relationships with the hydraulic resistance capacities of fine grained soil and coarse grained soil, respectively.