• 한국가축번식학회지
• /
• 제7권2호
• /
• pp.72-85
• /
• 1983

This presentation firstly is discussed the characteristics of estrus, the time of first postpartum estrus, and the relative accurate of various estrus detection aids and secondly discussed the abnormalities of estrus and ovarian function and its control by treatment of exogenous hormones in cattle and pig. Longer estrus cycles as well as the shorter than 18 day cycles showed the lowered conception rates as compared to the normal cycles of 18 to 25 days. Other characteristics of est겨s such as duration of estrus, intensity of estrus and time of estrus are reviewed to affect fertility. The first postpartum ovulation and estrus in cows usually occurs about 20 to 30 days and 40 to 50 days after parturition, respectively. Irregularities in estrus cycle length have been conducted during early postpartum period. In sows, weaning is followed by ovulation and estrus although there is some individual variation. The most common method of estrus detection is direct visual observation on standing estrus behavior, but various aids of estrus detection have been empolyed with varying degree of effectiveness. The results from heat detector devices are about as accurate as twice-daily observation(about 90%). The abnormal estrus can be classified into three types; irregular or continuous estrus, silent estrus and anestrus. Cystic ovarian disease, follicular cysts and luteal cysts, is a serious cause of reproductive failure in cattle and pig. The follicular cysts are much more common than luteal cysts and the incidence of ovarian cysts in dairy cattle is higher than beef cattle and pig. The occurrences of ovarian cysts have been closely associated with levels of milk production, stages of postpartum period, nutritional levels and seasons. The luteal cysts and persistent corpora lutea are responsive to the luteolytic effects of the recently synthetic analogues of PGF2$\alpha$ in cows and sows and recently GnRH or LH-RH has been successfully used as a treatment for cows and sows with ovarian follicular cysts.

• 한국수정란이식학회지
• /
• 제26권4호
• /
• pp.229-235
• /
• 2011

Sloan-Kettering virus gene product of a cellular protooncogene c-Ski is an unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. Ski plays multiple roles in a variety of cell types, it can induce both oncogenic transformation and terminal muscle differentiation when expressed at high levels. Ski protein is implicated in proliferation/differentiation in a variety of cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells, however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of this study was, by means of immunohistochemical methods, to locate Ski protein in the rat ovaries during ovulation and corpora lutea (CL) formation to predict the possible involvement of Ski in luteinization. In addition, we performed to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vivo models. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rat, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of corpus luteum (CL). These results indicate that Ski is profoundly expressed in the luteinized granulosa cells and luteal cells of CL during luteinization, and suggest that Ski may play a role in luteinization of granulosa cells.

• 한국수정란이식학회지
• /
• 제13권2호
• /
• pp.179-190
• /
• 1998

Prolactin (PRL) surge in cycling rats at proestrous afternoon has previously been reported as an inducer of apoptotic cell death of luteal cells. This death-inducing action of PRL seeins unusual, because PRL can he categorized as a cell-survival factor, if other known physiological functions of PRL are taken into account. In this study, the apoptotic action of PRL was assessed in cultured cells prepared from rat luteal tissue and underlying molecular /cellular mechanism of PRL-induced luteolysis was analyzed. The latest crop of corpora lutea (CLs) were enucleated from rat ovaries at 18:00 h on the proestrous day before the next ovulation. Donor rats were pretreated with CB154, a dopamine agonist, in order to he exempted from the endogenous PRL surge. The harvested GLs were dispersed and cultured with or without PRL (2$\mu$g /ml) for 24 or 48 h. An addition of PRL to the culture medium changed the parameters indicative of cell death via apoptosis: a decrease in cell viability (MTT) and an increase in chromatin condensation. Most of the DNA breakdown in nuclei induced by PRL occurred in steroidogenic cells which were identified by 3$\beta$-HSD activity staining, and the number of 3$\beta$-HSD-positivecells were significantly decreased. Interestingly, most of the cells with an apoptotic nucleus adhered to one or more intact and seemingly non-steroidogenic cells. Because the expression of Fas has heen shown to be abundant in murine ovary, and Fas is known to have an exact physiological role in occurrence of apoptotic cell death, the membrane form-Fas ligand (rnFasL) was quantified in the cell lysate. An addition of PRL increased expression of mFasL. Moreover, an addition of concanavalin A (ConA), a T-cell specific activator, in place of PRL, enhanced the apoptotic parameters. Cumulatively, the apoptotic PRL action was addressed to cells unknown than steroidogenic lute~ cells. The most prohable candidate for the direct target cells is Tcells in the luteal tissue that can express mFasL in response to PRL.

• 한국수정란이식학회지
• /
• 제17권2호
• /
• pp.123-128
• /
• 2002

The present study aimed at determining the effective dose of Folltropin, a follicle timulating hormone (FSH), on superovulation in indigenous cows of Bangladesh. Fifteen regularly cycling 5～7 years old dry cows, weighing 200～250 kg with 2.5～3.0 body condition scores (BCS) were divided into three groups (n＝5). Individual groups were superovulated with 100, 200 or 300 mg of Folltropin per animal. The superovulation treatment was initiated at Day 10 or Day 11 of the estrous cycle (Day 0＝day of estrus). Alfaprostol (6 mg) was injected to each cow 72 h after the initiation of superovulation treatment to induce eestrus. After confirming standing estrus, the cows were inseminated 2～3 times, 12 h apart, depending on the duration of estrus. At Day 6 or Day 7, individual horns of the uterus were flushed with 150～200 $m\ell$ of phosphate buffered saline supplemented with BSA (0.2％), penicillin (100 IU／$m\ell$) and streptomycin (100 $\mu\textrm{g}$／$m\ell$) using a two-way foley catheter. The embryos were concentrated, removing the excess medium through an embryo filter, and identified under a stereomicroscope. The identified embryos were collected, washed four times, evaluated and graded as excellent, good, fair or poor. The excellent, good and fair embryos were considered as transferable quality embryos. The mean (range). numbers of embryos collected vs. transferable quality embryos far 100, 200 and 300 mg of Folltropin were 4.5 (1～10) vs. 3.5 (1～8); 2.5 (1～4) vs. 1 (0～2) and 0.0 (0～0) vs. 0.0 (0～0), respectively, Folltropin at a dose of 100 or 200 mg produced suitable ovarian stimulation for superovulation in indigenous zebu cows of Bangladesh. A dose of 300 mg or more Folltropin consistently caused preovulatory corpora lutea formation in the ovaries and resulted in zero embryo recovery.

• 한국정보과학회논문지:소프트웨어및응용
• /
• 제35권10호
• /
• pp.627-635
• /
• 2008

본 연구에서는 사용자 어휘지능망(U-WIN)의 어휘 관계 중의 하나인 구문관계를 자동으로 구축하는 방법을 제시하고자 한다. 먼저, 구문관계를 형성할 수 있는 후보명사를 용언의 용례에서 문형 정보를 기준으로 추출함으로써, 용언의 세분화된 의미별로 정확하고 다양한 후보명사를 추출할 수 있다. 그러나 추출된 후보명사는 다양한 의미를 지니고 있으므로, 어휘간의 명확한 구문관계를 설정하기 위해서는 후보명사의 여러 의미 중에서 정확한 의미로 결정해야 한다. 그래서 본 연구에서는 용례 매칭 규칙, 구문 패턴, 의미 유사도, 빈도 정보 등을 이용하여 후보명사의 의미를 분별한다. 또한 구문패턴의 빈도 정보를 이용하여 용례에 나타나지 않지만 구문관계를 형성할 수 있는 명사를 추출하여 구문관계를 확장하고자 하였다. 이러한 연구는 명사 중심의 어휘망이 용언과의 구문관계 구축을 통해 형태소 분석, 구문 분석, 의미분석 등에 광범위하게 활용할 수 있는 어휘망의 기반을 다지는 작업이 될 수 있을 것이다.

• 한국어정보학회:학술대회논문집
• /
• 한국어정보학회 2016년도 제28회 한글및한국어정보처리학술대회
• /
• pp.195-200
• /
• 2016

본 연구는 MUSE 감성 코퍼스를 활용하여 문장의 극성과 키워드의 극성이 얼마만큼 일치하고 일치하지 않은지를 분석함으로써 특히 문장의 극성과 키워드의 극성이 불일치하는 유형에 대한 연구의 필요성을 역설하고자 한다. 본 연구를 위하여 DICORA에서 구축한 MUSE 감성주석코퍼스 가운데 IT 리뷰글 도메인으로부터 긍정 1,257문장, 부정 1,935문장을, 맛집 리뷰글 도메인으로부터는 긍정 2,418문장, 부정 432문장을 추출하였다. UNITEX를 이용하여 LGG를 구축한 후 이를 위의 코퍼스에 적용하여 나타난 양상을 살펴본 결과, 긍 부정 문장에서 반대 극성의 키워드가 실현된 경우는 두 도메인에서 약 4~16%의 비율로 나타났으며, 단일 키워드가 아닌 구나 문장 차원으로 극성이 표현된 경우는 두 도메인에서 약 25~40%의 비교적 높은 비율로 나타났음을 확인하였다. 이를 통해 키워드의 극성에 의존하기 보다는 문장과 키워드의 극성이 일치하지 않는 경우들, 가령 문장 전체의 극성을 전환시키는 극성전환장치(PSD)가 실현된 유형이나 문장 내 극성 어휘가 존재하지 않지만 구 또는 문장 차원의 극성이 표현되는 유형들에 대한 유의미한 연구가 수행되어야 비로소 신뢰할만한 오피니언 자동 분류 시스템의 구현이 가능하다는 것을 알 수 있다.

• 한국한의학연구원논문집
• /
• 제16권2호
• /
• pp.143-157
• /
• 2010

Objectives : The objective of this study was to access the effect of Artemisiae capillaris herba Extracts on the organogenetic reproductive toxicity of New Zealand White rabbits when administered by oral gavage. Methods : Gestational New Zealand White rabbits were dosed from 6 days of gestation to 18 days of gestation. It was conducted in accordance with the recommendations of the KFDA Guideline for Detection of Toxicity to Reproduction for Medicinal Products. In addition, serum AST, ALT, LDH and ALP levels were detected with their necropsy and histopathological observation Results: No significant changes of body weights, gains and food consumption were demonstrated in all dosed groups compared to those of vehicle control group except for 2,000mg/kg-dosing group, which showed significantly increase of body weight compared to that of vehicle control group. Normal gross findings were demonstrated in all tested groups. No significant changes of number of corpora lutea, implantation, implantation rate, number of fetal death, loss rate of post-implantation, number of live youngs at C-section, survival rate of post-implantation, number of male live youngs at C-section, number of female live youngs, sex ratio of live young, external anomalies of live youngs and body weight of live youngs were demonstrated in all dosed groups compared to those of vehicle control group. Although some visceral variations such as thymic reminant in the neck, dilation of renal pelvis, dilated ureter and malpositioned left common carotid artery, no significant changes were demonstrated in all dosed groups compared to that of vehicle control group. Some malformations and variation were demonstrated with retardations. However, no significant changes were demonstrated in all dosed groups compared to that of vehicle control group. In addition, similar ossification numbers were detected in sternebrae, metacarpals, metatarsals, phalanges, cervical vertebrae, and sacral and caudal vertebrae. No Artemisiae capillaris herba Extracts treatment-related changes of serum AST, ALT, LDH and ALP levels were demonstrated in all dosed levels in this study. Conclusions : We could conclude that oral administration of Artemisiae capillaris herba Extracts hasn't an influence on the organogenetic reproductive toxicity.

• 정보처리학회논문지B
• /
• 제13B권4호
• /
• pp.479-488
• /
• 2006

언어 장애인에게 기존의 키보드에 의한 문자입력 방법보다 원활하고 편리한 의사전달 환경을 제공하기 위한 아이콘 언어 인터페이스를 설계한다. 이를 위하여 활용성이 높은 대화 영역으로부터 구축한 원시 말뭉치를 대상으로 어휘 구사 경향과 특성을 분석하고 형태소, 구문, 의미 분석을 적용하여 아이콘에 부여되는 한국어의 어휘와 의미를 추출한다. 사용자가 직관적으로 인지하고 전달할 수 있는 아이콘 영역을 선별하고 추출한 한국어의 어휘와 의미를 정합시킨다. 이웃하는 아이콘간의 연결로부터 전달하고자 하는 의미적 상황을 만들어내기 위하여, 아이콘 언어의 어휘와 품사, 문법 규칙, 의미체계를 정의하여 아이콘 언어를 설계한다. 아이콘 언어에서 나타날 수 있는 언어적 애매성을 해결하기 위한 방법으로 범용의 한국어 의미사전과 술어 중심의 하위범주화사전으로부터 아이콘 언어에 대한 상황중심의 의미 데이터를 구축한다. 이를 바탕으로 아이콘 언어 인터페이스로부터 한국어를 의미적인 범주에서 생성한다.

• 대한한의학회지
• /
• 제26권2호
• /
• pp.32-51
• /
• 2005

Objectives: To study the effect of Artemisiae Capillaris Herba extracts, that have been used as oriental medicine to treat liver disease, on the perinatal and lactational n;)productive toxicity of SD rats when administered by oral lavage. Methods: Female SD rats were dosed from 6 days of gestation to 3 weeks postpartum. This was conducted in accordance with the recommendations of the KFDA Guidelines for Detection of Toxicity to Reproduction for Medicinal Products. Results: No Artemisiae Capillaris Herba extracts treatment-related changes in clinical signs, mortalities, implantation number, dead fetus number, loss rate of fetus, number of live young, survival rate of fetus, sex ratio of live young, external anomalies, pregnancy periods, viability index, lactational index, survival rate of litters at 4 days after birth or delivery index were demonstrated in any dosed levels in this study. However, the body weight and gains, food consumption and absolute organ weights of brain, adrenal glands, liver, spleen, kidney, ovaries and heart were significantly increased in 2000 or 1000mg/kg-dosing groups and the relative organ Weights of adrenal glands were significantly increased in 2,000mg/kg-dosing groups. Therefore, it was concluded that this increase was natural according to growth. Also, no changes of gross findings, clinical signs, mortalities, body weight and gains, physical development results, necropsy findings, organ weight, faculty test, open filed test and water-filled simple T-maze test, copulation, fertility, pregnancy indices, body weight and gains during gestation periods, necropsy findings, corpora lutea number, implantation number, implantation rate, dead fetus number, post-implantation loss rate, live young, post-implantation survival rate, sex ratio of live young, external anomalies and individual body weights of live young were demonstrated in any dosed levels in this study. Conclusions: It is considered that the NOAEL (No-Observed-Adverse-Effect Level) for perinatal and lactational reproductive toxicity of Artemisiae Capillaris Herba extracts was up to 2000mg/kg/day because no changes of other perinatal and lactational reproductive indices were demonstrated.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권1호
• /
• pp.65-70
• /
• 1998

To understand the caused for poor response to superovulation in water buffalo compared to crossbred cows, follicular events, before start of superovulation, during superovulation and after superovulation were compared. Follicular development was monitored a day before start of superovulation, daily upto superestrus and on the day of flushing. A real time B mode diagnostic instrument equipped with a linear array, 5 MHz transducer was used in five crossbred cows and five Murrah buffaloes. Crossbred cows yielded significantly (p < 0.01) higher number of corpora lutea than buffaloes (21 vs 10). The mean number of small size (2 to 5 mm); medium size (6 to 9 mm) and large size $({\geq}10mm)$ follicles, a day before start of superovulation were almost similar or even slightly higher in buffalo. Though initial shift in the mean number of follicles was higher in buffalo than cow, yet, from Day 2 to Day 3 of the treatment, the average increase in medium (3.2 vs 1.2) and large size (5.0 vs 2.0) follicles was higher in cows than buffaloes. The mean number of medium and large size follicles was 9.8 and 14.4 in cows and 6.4 and 7.6 in buffaloes. On the day of flushing, the number of large size follicle was more in buffaloes than cows, indicating the ovulation problem in this species. The major conclusion from this investigation was that, a day before start of superovulatory treatment, the number of small and medium size follicles was slightly higher in buffaloes, even then superovulatory response was better in cows, due to shift, recruitment and passage of follicles from smaller size to larger size from Day 2 of treatment. Ovulation problem in buffaloes was also responsible for lower superovulatory responses as revealed by the presence of higher number of large size follicles on the day of flushing.