• Journal of Pharmacopuncture
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• v.11 no.2
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• pp.81-85
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• 2008

Objective This study was done to investigate the safety of Bovis Calculus pharmacopuncture solution manufactured with freezing dryness method to use eye drop. Methods The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration (2005. 10. 21, KFDA 2005-60). After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, the auther observed eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Results 1. After Bovis Calculus pharmacopuncture solution was medicated in the left eye of the rabbits, there wasn't physical problem at 9 rabbits. 2. After Bovis Calculus pharmacopuncture solutionwas medicated in the left eye of the rabbits, there wasn't eye irritation of the cornea, iris, conjunctiva at 1, 2, 3, 4 & 7day. Conclusions I suggested that Bovis Calculus pharmacopuncture solution didn't induced eye irritation in rabbits.

• Proceedings of the KOSOMBE Conference
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• v.1994 no.05
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• pp.127-130
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• 1994

This study is focused on investigating the mechanical properties of the human cornea and its shape through the inverse nonlinear finite element approach using the clinical and experimental data. The results of the inverse approach were used to construct the finite element model of the photorefractive excimer laser surgery for the myopia patients. The results of the finite element model were compared with those of the current clinical experiences and showed good agreements. Finally this study came to the conclusion that the finite element method has potential of the application to the medicine.

• Applied Microscopy
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• v.24 no.4
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• pp.98-106
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• 1994

The corneal formation of compound eye of Pieris rapae L., which was mostly made during pupal stage, was morphologically investigated with light microscope, scanning electron microscope and transmission electron microscope. The regeneration of the microvilli were found on the surface membranes of corneagen cells and retinular pigment cells of preommatidium after apolysis pupal cuticle. The microvilli were finally differentiated to corneal nipples of the ommatidium. The corneal cuticle was generated on the superficial layer of the preommatidium from corneagen cells and retinular pigment cells. The corneal process was also formed under the cuticular layer from the corneagen cells. The pore canal was appeared within the cuticular layer and connected with the retinular pigment cell as if the root of interommatidial hair was connected. The interommatidial hair was projected randomly among the ommatidial facets and cornal nipple was arrayed regular on the ommatidial facets. The cornea was convex lens and the refracting power by its convex shape was 4 diopter.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.289-292
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• 2002

The corneal tissue consists of three layers : epithelium, stroma, and endothelium. Central cornea is a highly differentiated tissue whereas the limbus contains the epithelial stem cell. In the present study. we report the engineering of the three-dimensional reconstructed cornea derived from rabbit limbal epithelial and stromal cells. The differentiation degree of corneal stem cells were assessed in serum concentration and inoculation density of stromal cells. Optimal condition differentiation of corneal stem cells is achieved when 5% FBS was supplemented to culture medium and $1-2{\times}10^5$ cells/ml inoculation density of stromal cells.

• Journal of Veterinary Clinics
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• v.19 no.4
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• pp.415-417
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• 2002

To investigate the diurnal change of intraocular pressures(IOP) and compare of $Tono-pen^{XL}$ and Schi$\emptyset$tz tonometer, we measured the IOP in clinically normal eyes of dogs. To estimate the diurnal change of IOP, the measurements of IOP were taken with $Tono-pen^{XL}$ in 44 normal canine eyes. Two physicians measured the IOP through the central cornea and limbal cornea. To compare of two tonometers, a physician measured the IOP with both $Tono-pen^{XL}$ and Schi$\emptyset$tz (7.5 g) tonometer in 23 clinically normal eyes of dogs. Mean of lOP was 26.51 $\pm$ 6.91 mmHg and IOP was highest as 30.65 mmHg at a.m. 10, but not significantly different within physicians and corneal regions (p > 0.05). Correlation coefficient within two tonometers was too low.

• Journal of The Korean Society of Inherited Metabolic disease
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• v.22 no.2
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• pp.37-45
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• 2022

Inherited metabolic disorders (IMD) are a large group of rare disorders affecting normal biochemical pathways. The ophthalmic involvement can be very varied affecting any part of the eye, including abnormalities of cornea, lens dislocation and cataracts, retina and the optic nerve, and extraocular muscles. Eye disorders can be initial symptoms of some IMD and can be clue for diagnosis of IMD. However, eye disorders can evolve later in the natural history of an already diagnosed metabolic disorder. Awareness of IMDs is important to facilitate early diagnosis and in some cases instigate early treatment if a patient presents with eye involvement suggestive of a metabolic disorder. Ophthalmological interventions are also an important component of the multisystem holistic approach to treating patients with metabolic disorders.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.27 no.2
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• pp.59-67
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• 2001

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.3
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• pp.262-269
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• 2005

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

• Biomedical Science Letters
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• v.14 no.3
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• pp.173-178
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• 2008

With the widespread use of laser in medical and industrial settings, the incidence of laser injury to the ocular continues to grow among workers involved in handling lasers. The aim of this study is to compare ocular damages after irradiation with various laser wavelengths and power density. Ocular of pigmented rats was irradiated with $CO_2$ laser, 1064 nm Nd:YAG laser, and 532 nm diode laser. We observed damage of cornea, lens, and retina using slit lamp microscope and funduscopy. H&E staining of histopathology were applied to study the specimens. The higher exposure ($200mW/cm^2$, 10 sec) with $CO_2$ laser resulted in severe damage at the cornea. For the 1064 nm Nd:YAG laser, the higher exposure than $10mW/cm^2$ (10 sec) resulted in damage at the cornea and lens. Further, with the 532 nm diode laser, retinal lesions were induced when $10mW/cm^2$ (0.25 sec) was delivered to the eye. Theses results suggest that the ocular damages are different from various laser wavelength and power density.

• ALGAE
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• v.31 no.3
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• pp.243-256
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• 2016

Concerns about how ocean acidification will impact marine organisms have steadily increased in recent years, but there is a lack of knowledge on the responses of macroalgae. Here, we adopt an outdoor continuous-flowing mesocosm system designed for ocean acidification experiment that allows high CO₂ conditions to vary with natural fluctuations in the environment. Following the establishment of the mesocosm, five species of macroalgae that are common along the coast of Korea (namely Ulva pertusa, Codium fragile, Sargassum thunbergii, S. horneri, and Prionitis cornea) were exposed to three different CO₂ concentrations: ambient (×1) and elevated CO₂ (2× and 4× ambient), over two-week period, and their ecophysiological traits were measured. Results indicated that both photosynthesis and growth exhibited species-specific responses to the different CO₂ concentrations. Most notably, photosynthesis and growth increased in S. thunbergii when exposed to elevated CO₂ conditions but decreased in P. cornea. The preference for different inorganic carbon species (CO₂ and HCO₃⁻), which were estimated by gross photosynthesis in the presence and absence of the external carbonic anhydrase (eCA) inhibitor acetazolamide, were also found to vary among species and CO₂ treatments. Specifically, the two Sargassum species exhibited decreased eCA inhibition of photosynthesis with increased growth when exposed to high CO₂ conditions. In contrast, growth of U. pertusa and C. fragile were not notably affected by increased CO₂. Together, these results suggest that the five species of macroalgae may respond differently to changes in ocean acidity, with species-specific responses based on their differentiated photosynthetic acclimation. Understanding these physiological changes might allow us to better predict future changes in macroalgal communities in a more acidic ocean.