• 생약학회지
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• 제26권4호
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• pp.411-418
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• 1995

In order to clarify the originality of several Alisma Rhizomes, we investigated the differences of their morphological, anatomical and physiochemical characteristics. Morphological observation shows the variability of Alisma shape and quality. The diversities in morphological shapes and qualities of tuber were observed among different origins. Chinese product looked like corm shape, but korean's irregular shape. The microscopic observation of korean Alisma revealed the presence of irregular vascular bundle which was scattered transversely and longitudinaly, which was different from that of the rhizome. This facts suggest that the portion of crude drug in Alisma should be the corm rather than the rhizome. In the physicochemical differences, chinese Alisma contains larger amount of mineral elements such as K, Na and Ca than korean Alisma. On the contrary, protein contents in lyophilized powder of aqueous extracts in korean Alisma rhizome was significantly higher than chinese Alisma rhizome. The leaves and stems contained larger amount of Ca and Na than tuber, and may be used as the osmotic diuretics for chinese traditional medicine.

• 원예과학기술지
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• 제33권6호
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• pp.812-819
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• 2015

Gladiolus is one of the most popular cut flowers in the world floral market and is highly attractive to consumers. The production of poor-quality gladiolus spikes is one of the most important hurdles to obtaining high prices for this crop. The main objectives of this study were to improve the quantitative characteristics of gladiolus spikes, to increase propagule production and to enhance chemical constituent levels in leaves to help improve plant growth. We performed foliar application of bioregulators, including polyamines (putrescine and spermine) and vitamins (ascorbic acid and thiamine), at different concentrations (0, 0.1, 0.4, 0.7 and 1 mM) to gladiolus plants at the three leaf, five leaf and slipping stages. Application of 1 mM putrescine was the most effective treatment, with prominent effects on economically important spike traits including spike length (55.53 cm), spike diameter (7.53 mm), florets per spike (13.99), first floret diameter (9.66 cm) and first floret weight (4.90 g), followed by spermine treatment. The 1 mM putrescine treatment also had positive effects on corm-associated traits including corm diameter and corm weight, with values of 4.57 cm and 26.16 g compared to 3.47 cm and 17.16 g in control plants, respectively. The contents of chemical constituents in leaves, including total chlorophylls ($8.06mg{\cdot}g^{-1}$), total carotenoids ($1.66mg{\cdot}g^{-1}$), total soluble sugars ($4.75 mg{\cdot}g^{-1}$) and phenolics ($0.89mg{\cdot}g^{-1}$) increased in response to foliar application of 1 mM putrescine compared to the control. Ascorbic acid and thiamine also had positive effects on various plant morphological traits and chemical constituents in leaves compared to control plants, but the growth-promoting effects of polyamines were more pronounced. Hence, applying polyamines to gladiolus leaves will help improve spike production and increasing propagule (cormel) yields.

• 동의생리병리학회지
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• 제19권6호
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• pp.1541-1545
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• 2005

The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Corm fructus. The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being $200{\mu}g/mL$. The extract at concentrations of $10-500{\mu}g/ml$ showed dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (63.0%) was blocked by the extract (10, 50, 100, 250 and $500{\mu}g/ml$) concentration-dependently. Furthermore, the extract (50, 100 and $250{\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Corm fructus has potent antioxidant activity.

• 화훼연구
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• 제18권2호
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• pp.142-146
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• 2010

순백색 바탕에 자주색 화심이 있는 조생종 글라디올러스 '아리랑'이 1998년 국립원예특작과학원(구 원예연구소)에 의해 개발되었다. '아리랑'은 어두운 오렌지색 조생종 '아리안느'에 밝은 홍적색 대형종 국산품종인 '홍광'을 교배한 조합에서 선발되었다. 1993년에 교배하여 얻은 실생 종자를 파종, 1994년에 처음 선발하였으며 1998년까지 5년에 걸쳐 생육특성검정을 하였다. 아리랑은 밝은 백색바탕에 자주색 화심이 있는 절화용 품종으로 조생종이면서 꽃대가 휘지 않는 강한 초세를 갖고 있다. 따라서 여름철 노지 재배용으로 이용이 가능하다. 또한 장마철 습해에도 강하고 구근부패병에 강해 장마철 절화가 가능한 품종이다. 생육속도는 매우 빠르며 구근 번식력도 좋다. 아리랑의 여름철 개화소요일수는 75일 정도이며 구근 생산재배 시 품질 좋은 구근생산을 위해 생육 기간 중 추비를 1-2회 뿌려 주는 것이 좋다.

• 한국자원식물학회지
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• 제14권3호
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• pp.229-234
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• 2001

토란 재배시 관행적으로 이용가치가 적어서 버리는 설우(屑芋를 종우(種芋)로 활용하는 방법을 연구하고자 본 시험을 실시한 결과는 다음과 같다. 1. 토란의 출현은 종우(種芋)의 크기별로 약간의 차이가 인정되어 자우(子芋)인 설우(屑芋)나 소우(小芋)가 친우(親芋)보다 약간 빠른 경향이었으나 출현율은 친우가 높았다. 2. 생육은 자우(子芋)보다는 친우가 엽병장이 크고 엽병속수(葉炳束數)가 많아 양호하였으며, 자우(子芋)가 클수록 생육이 양호한 경 향이었다. 자우(子芋)중 설우(屑芋)도 엽병속이 1.5~2.6개 발생되어 정상적인 생장이 가능하였다. 3.토란 종우(種芋)의 크기에 따른 괴경(塊莖)과 부산물(副産物) 엽병(葉炳)의 수량은 자우(子芋)의 크기간에 차이가 인정되어 자우(子芋)가 클수록 수량이 증수되는 경향이었다. 또한 설우(屑芋)도 크기에 따라 10a 당730~1,680kg의 괴경(壞莖)과 100~310kg의 엽병(葉炳)을 생산할 수가 있어서 대면적의 재배시 종우(種芋)로 이용이 가능할 것으로 생각된다.

• Biomolecules & Therapeutics
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• 제29권2호
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• pp.175-183
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• 2021

The mitogen-activated protein kinase (MAPK) pathway controls intestinal epithelial barrier permeability by regulating tight junctions (TJs) and epithelial cells damage. Heme oxygenase-1 (HO-1) and carbon monoxide (CO) protect the intestinal epithelial barrier function, but the molecular mechanism is not yet clarified. MAPK activation and barrier permeability were studied using monolayers of Caco-2 cells treated with tissue necrosis factor α (TNF-α) transfected with FUGW-HO-1 or pLKO.1-sh-HO-1 plasmid. Intestinal mucosal barrier permeability and MAPK activation were also investigated using carbon tetrachloride (CCl4) administration with CoPP (a HO-1 inducer), ZnPP (a HO-1 inhibitor), CO releasing molecule 2 (CORM-2), or inactived-CORM-2-treated wild-type mice and mice with HO-1 deficiency in intestinal epithelial cells. TNF-α increased epithelial TJ disruption and cleaved caspase-3 expression, induced ERK, p38, and JNK phosphorylation. In addition, HO-1 blocked TNF-α-induced increase in epithelial TJs disruption, cleaved caspase-3 expression, as well as ERK, p38, and JNK phosphorylation in an HO-1-dependent manner. CoPP and CORM-2 directly ameliorated intestinal mucosal injury, attenuated TJ disruption and cleaved caspase-3 expression, and inhibited epithelial ERK, p38, and JNK phosphorylation after chronic CCl4 injection. Conversely, ZnPP completely reversed these effects. Furthermore, mice with intestinal epithelial HO-1 deficient exhibited a robust increase in mucosal TJs disruption, cleaved caspase-3 expression, and MAPKs activation as compared to the control group mice. These data demonstrated that HO-1-dependent MAPK signaling inhibition preserves the intestinal mucosal barrier integrity by abrogating TJ dysregulation and epithelial cell damage. The differential targeting of gut HO-1-MAPK axis leads to improved intestinal disease therapy.

• 한국자원식물학회지
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• 제15권2호
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• pp.172-176
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• 2002

토란 재배시 종우가 부족할 경우 절편자우를 종우로 활용하는 방법을 연구하고자 본 시험을 실시한 결과는 다음과 같다. 1. 토란의 출현은 절편자우의 크기 별로 차이가 인정되지 않았고, 출현율은 90% 이상 출현되었다. 2. 생육은 절편자우가 클수록 엽병장이 크고 엽병속수가 많아 양호하였다. 3. 토란 절편자우의 크기에 따른 괴경과 부산물인 엽병의 수량은 크기간에 유의성이 인정되어 절편자우가 클수록 수량이 증수되는 경향으로 절편자우 20g을 종우로 사용할 경우 10g당 1,981kg을 생산할 수가 있었다. 이와 같은 결과로 보아 절편자우가 20g 이상인 것을 종우로 사용할 경우 토란의 재배가 가능할 것으로 본다.

• The Korean Journal of Physiology and Pharmacology
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• 제25권3호
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• pp.227-237
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• 2021

Carbon monoxide (CO) is a cardioprotectant and potential cardiovascular therapeutic agent. Human cardiac fibroblasts (HCFs) are important determinants of myocardial structure and function. Large-conductance Ca2+-activated K+ (BK) channel is a potential therapeutic target for cardiovascular disease. We investigated whether CO modulates BK channels and the signaling pathways in HCFs using whole-cell mode patch-clamp recordings. CO-releasing molecules (CORMs; CORM-2 and CORM-3) significantly increased the amplitudes of BK currents (IBK). The CO-induced stimulating effects on IBK were blocked by pre-treatment with specific nitric oxide synthase (NOS) blockers (L-NG-monomethyl arginine citrate and L-NG-nitroarginine methyl ester). 8-bromo-cyclic GMP increased IBK. KT5823 (inhibits PKG) or ODQ (inhibits soluble guanylate cyclase) blocked the CO-stimulating effect on IBK. Moreover, 8-bromo-cyclic AMP also increased IBK, and pre-treatment with KT5720 (inhibits PKA) or SQ22536 (inhibits adenylate cyclase) blocked the CO effect. Pre-treatment with N-ethylmaleimide (a thiol-alkylating reagent) also blocked the CO effect on IBK, and DL-dithiothreitol (a reducing agent) reversed the CO effect. These data suggest that CO activates IBK through NO via the NOS and through the PKG, PKA, and S-nitrosylation pathways.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.62-62
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• 2022

Freesia has been an important worldwide cut flower because of its fragrance, long vase life and the wide color range of the flower. The conventional propagation methods by seeds and corms have many disadvantages such as shorter inflorescences with fewer numbers of florets, a reduction in cut flower quality and the accumulation of plant viruses in corms by successive cultivation. Therefore, the conventional propagation systems in Freesia needs to be replaced with tissue cultures to overcome the disadvantages. This study explored an efficient multiplication protocol using the combination of plant growth regulators (PGRs) for developed cultivar 'Sunny Gold'. The combination between 6-benzylaminopurin (BA) and α-naphthalene acetic acid (NAA) did not produce new shoots but developed enlarged roots. BA only treatments and the combination between BA and kinetin treatments were effective on shoot multiplication. The highest average number of shoots was 5.3 in the presence of 3 mg/L BA and 0.5 mg/L kinetin. To produce corms and cormlets, proliferated shoots were subcultured on 1/2 Murashige and Skoog (MS) medium supplemented with 90 g/L sucrose, 1 g/L charcoal and 7 g/L plant agar and placed at 4℃ in the dark for 6 months. The small size of corms and comlets were produced. The average number of regenerated comlets was 2.75 per shoot. The results showed that shoot multiplication is more efficient than cormlet regeneration for in vitro freesia proliferation.

• 한국잡초학회지
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• 제11권2호
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• pp.111-116
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• 1991

본(本) 연구(硏究)에서는 간척지(干拓地)의 우점잡초(優占雜草)인 새섬매자기의 방제(防除)를 위한 기초자료(基礎資料)를 얻고자 새섬매자거의 번식(繁殖)에 관한 생태적(生態的) 특성(特性)을 조사(調査), 검토(檢討)하였다. l. 새섬매자기의 구경(球莖)은 토양심도(土壤深度) 0-5cm에 16%, 5-8cm에 66% 그리고 8-10cm에 18% 각각 분포(分布)되였으며 평균분포심도(平均分布深度)는 6.51cm였다. 2. 구경(球莖)당 근경(根莖) 및 신아(新芽)의 수(數)는 각각 1개(個)에서 4개(個)까지로 근경(根莖)은 평균(平均) 2.34개(個), 신아(新芽)는 평균(平均)2.85개(個)였으며 전체(全體) 눈 (근경(根莖)과 신아(新芽))의 수(數)는 3-7개로 평균(平均) 5.2개(個)였다 3. 엽서(葉序)는 1/3이였으며 총(總) 12엽중(葉中) 4엽(葉)까지는 포엽(苞葉)이었다. 4. 파종구경(播種球莖)으로부터 출아주(出芽株)가 100주(株)로 증식(增植)되기까지의 일수(日數)는 3월(月) 25일(日) 출아(出芽)를 시작한 구경(球莖)에서 58일(日), 5월(月)7일(日) 출아구경(出芽球莖)에서 52일(日), 그리고 6월(月) 6일(日) 출아구경(出芽球莖)에서 43일(日)이 각각 소요(所要)되었다. 5. 파종구경(播種球莖)으로부터 번식(繁殖)에 따른 출아(出芽)(신구경발달(新球莖發達) 차수(次數)가 증가(增加)할수록 다음 출아(出芽)까지의 일수(日數)는 단축(短縮)되었고 근경장(根莖長), 초장(草長) 및 줄기의 지름은 점차(漸次) 증가(增加)되었다. 6. 초장(草長)은 직파재배(直播栽培)에서 재배초기(栽培初期)부터 벼보다 빠르게 신장(伸長)되었으며 이앙재배(移秧栽培)에서는 이앙후(移秧後) 약 30일(日)부터 벼의 것을 능가하였다. 7. 새섬매자기의 번식속도(繁殖速度)는 이앙재배(移秧栽培)보다 직파재배(直播栽培)에서 빨랐다.