Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Antioxidant Effects of Corni Fructus in GC-1 Cells

산수유(山茱萸)가 남성 생식세포 GC-1의 항산화에 미치는 영향

  • Oh Myung-Sook (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Do-Rim (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Sung Eun-Jin (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang Mun-Seog (Department of Medicine, Division of Newborn Medicine, Children's Hospital and Harvard Medical School) ;
  • Park Seong-Kyu (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 오명숙 (경희대학교 한의과대학 방제학교실) ;
  • 김도림 (경희대학교 한의과대학 방제학교실) ;
  • 성은진 (경희대학교 한의과대학 방제학교실) ;
  • 장문석 (하버드대학교 의과대학 소아병원) ;
  • 박성규 (경희대학교 한의과대학 방제학교실)
  • Published : 2005.12.01
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Abstract

The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Corm fructus. The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being $200{\mu}g/mL$. The extract at concentrations of $10-500{\mu}g/ml$ showed dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (63.0%) was blocked by the extract (10, 50, 100, 250 and $500{\mu}g/ml$) concentration-dependently. Furthermore, the extract (50, 100 and $250{\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Corm fructus has potent antioxidant activity.

Keywords

References

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