• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1760-1768
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• 2020

Vibrio cholerae, cause of the life-threatening diarrheal disease cholera, can be divided into different serogroups based on the structure of its lipopolysaccharide (LPS), which consists of lipid-A, core-polysaccharide and O-antigen polysaccharide (O-PS). The O1 serogroup, the predominant cause of cholera, includes two major serotypes, Inaba and Ogawa. These serotypes are differentiated by the presence of a single 2-O-methyl group in the upstream terminal perosamine of the Ogawa O-PS, which is absent in the Inaba O-PS. To ensure the consistent quality and efficacy of the current cholera vaccines, accurate measurement and characterization of each of these two serotypes is highly important. In this study, we efficiently screened a phage-displayed human synthetic Fab library by bio-panning against Ogawa LPS and finally selected three unique mAbs (D9, E11, and F7) that specifically react with Ogawa LPS. The mAbs bound to Vibrio cholerae vaccine in a dose-dependent fashion. Sequence and structure analyses of antibody paratopes suggest that IgG D9 might have the same fine specificity as that of the murine mAbs, which were shown to bind to the upstream terminal perosamine of Ogawa O-PS, whereas IgGs F7 and E11 showed some different characteristics in the paratopes. To our knowledge, this study is the first to demonstrate the generation of Ogawa-specific mAbs using phage display technology. The mAbs will be useful for identification and quantification of Ogawa LPS in multivalent V. cholerae vaccines.

• Journal of the East Asian Society of Dietary Life
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• v.26 no.4
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• pp.346-358
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• 2016

Local food reflects the identity and image of a specific region. It is also a core element for determining tourists' experience of a region. The purpose of the present study was to develop a Jeju food tour package using local foods and estimate the economic value of the suggested tour package using Contingent Valuation Method (CVM). Five food tour packages were developed by researchers. In order to modify and measure the value of each package, an expert panel survey was conducted, and 'package 5' was selected as a food tour scenario for the tourist survey. A survey was conducted on 295 domestic tourists who visited Jeju within the past 10 years in order to evaluate the economic value and feasibility of the Jeju food tour package. A total fo 72.9% of respondents answered that they were willing to pay for the Jeju local food tour, and the willingness to pay (WTP) range was between ￦45,000 and ￦105,000. The primary factors correlated with WTP was food expenses, followed by leisure experience expenses, interest in local foods, education level, visit frequency and age. The WTP of the Jeju local food tour was calculated with the significantly affected factors using stepwise regression model. The result of the present study reveal that tourists' WTP increased with higher food expenses, education level, and age group. The average value of food expenses, education level, and age were substituted into the formula derived from the regression analysis, yielding 58,385.752 KRW as the WTP. The expected economic value created by exploiting the Jeju local food tour was shown to be 700 billion KRW per year, calculated by multiplying WTP of the food tour packages by the number of tourists. This study examined the feasibility and plan of the food tour package to increase the economic value of Jeju local food. In the case of the culinary tour program based on Jeju local food launching, the estimated economic effect was great. Therefore, in-depth research to merchandise the Jeju local food tour program is needed.

• Korean Journal of Agricultural Science
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• v.39 no.4
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• pp.459-466
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• 2012

The objective of this study is to observe growth pattern of grand rapids(lettuce) according to different dexperimental conditions for minimizing its growth period such as composition of nutrient, irradiation time of light source and cultivation mode. The culture of water spray type using seeds of grand rapids was performed during growth period of total 50 days. Experimental conditions consist of five compositions of nutrient (PW 0.5 mS/cm, PW 1.0 mS/cm, PY 0.5 mS/cm, PY 1.0 mS/cm, PW 2.0 mS/cm) and three irradiation times of light source (12h(on) / 12h(off), 18h(on) / 06h(off), 24h(on) / 00h(off)). Illumination was adjusted to ratio of 7:1:1 of red, blue and white color, respectively. Indoor environmental condition for cultivating grand rapids is as follows: temperature (19-$22^{\circ}C$), relative humidity (60-70%) and carbon dioxide (1,000-1,200 ppm). The data were obtained from five iteration tests. The maximum growth level was observed in the experimental condition of 18 hr(on) / 06 hr(off) and PY 1 mS/cm for 1st week of cultivation period, 24 hr(on) / 00 hr(off) and PY 1mS/cm for 2nd week of cultivation period, and 24 hr(on) / 00hr(off) and PW 1 mS/cm for 3rd week of cultivation period, respectively. On the contrary, the minimum growth level was observed in the experimental condition of 18 hr(on) / 06 hr(off) and PW 0.5 mS/cm for 1st week of cultivation period, 12 hr(on) / 12 hr(off) and PW 0.5 mS/cm for 2nd week of cultivation period, and 12 hr(on) / 12 hr(off) and PY 0.5 mS/cm for 3rd week of cultivation period, respectively. Based on the results obtained from this study, it is concluded that the growth level of grand rapids varied with different irradiation time of light source and composition ratio of nutrient according to cultivation period.

• Journal of architectural history
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• v.3 no.1
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• pp.57-82
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• 1994

This paper is the result of the researches and the field surveys of the villages and the dwellings of Korean immigrants in Yien-Pien area, north-eastern China. This study aims to persue both of the origin and the process of development of their settlements and dwelling types from late 19C to the present. Their processes are too complex to analysis by single view-point. I have eyes to interprete them from three pionts; 1)correspondences between the dwelling types and the econo-political history of their region, 2)cultural assimilation with the native dwelling types, and 3)the direction of their modernization with the economical development of modern China. Three village types have been pioneered; 1)the villages of indivisual immigration, 2)the villages of planned group immigration, and 3)the villages of socilistic reform villages of 1) were composed of organic village patterns and various shaped dwelling lots on the sloped site; villages both of 2) and 3), gird patterns and uniformed lots on open fields. Historically, villages of 1) were pioneered before 1931; villages of 2), 1936-1945; villages of 3), from 1945. Each of dwelling types had strong relations with the village types to which it belonged. Before 1931, dwellings were built up based on so called "Ham-buk dwelling type" which was dominent in north-eastern Korea. In the era of gruop-immigration, various dwelling types were flew into Yen-Pien from southern Korea. In modern China, their southern types were changed into Yen-Pien type as similar as Ham-book type. After 1945, with the Great leap Forward and the Cultural Revolution, as communization of indivisual properties and reorganization of rural communities, each of dwellings became smaller and simpler in aspects of scales as well as functions. There are two types in Yen-Pien dwellings, those are 'single-file' and 'double-file' type. Three sub-types of latter arc 'six-bays', 'eight-bays', and rarely 'ten-bays'. The most common element of all types is Chong-ju-k'an; which is large room with heated floor, openig to kitchen. Now, modern dwellings of Korean immigrants are changing their spatial compositions, materials, and structures. With cultural assimilation as well as modernization, especially in urban areas, they are compelled to accept the elements of Chinese dwellings. But the spatial element of "Chong-ju-k'an", which is the core element of Yen-Pien dwelling type, never fade away nor is changed.

• Korea journal of population studies
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• v.28 no.2
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• pp.131-164
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• 2005

The central focus of this research paper is to explore the recent structural core empirical unemployment reason of IT new professional manpower who graduated in higher education level in Korea. The final goal of this issue is to estimate and analyze rambling impediment in IT manpower labor market between 26 detail occupations and between detail occupations and 3 educational levels of new graduates and entrants who entered in IT 6 occupational groups composing 26 particular occupations. This study uses the 2004 data published by the Ministry of ICT and relies upon both one-way analysis of variance and two-way analysis variance to tackle the issue. According to the ANOVA results, 2 main points were delineated In the one-way analysis of variance method. the total sum of squares (SST) is decomposed into two major components: within sum of squares (SSW) (6 detail occupations, 85%) and between sum of squares (SSG) (26 detail occupation, 15%) The other major finding is that total sum of squares (SST) is decomposed into three major components: between detail occupations (SSG) (19.3%), between educations (SSB) and errors (SSE) (56%) in IT SIW development design group.

• Perspectives in Nursing Science
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• v.10 no.2
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• pp.87-96
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• 2013

Purpose: The purpose of this study was to investigate the relationships of activity status, anxiety, depression, social support, symptom experience, and functional status in patients with lung cancer based on the theory of unpleasant symptoms. Methods: The participants for this study were 101 lung cancer patients who visited the out-patient department for treatment or follow-up at one hospital in Seoul. Data were collected from January 1 to February 8, 2013 using self-reported questionnaires and clinical records. To measure variables, the functional scale and symptom scale of the European Organization for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire-Core 30, EORTC QLQ-Lung Cancer 13, Eastern Cooperative Oncology Group Performance Status, Hospital Anxiety and Depression Scale, and Multidimensional Scale of Perceived Social Support were used in this study. The data were analyzed using SPSS 19.0 software for Windows. Results: The symptom experience showed more severity in patients with lower activity status, higher anxiety and depression. With lower activity status and social support, functional status was lower. When anxiety, depression, and symptom experience were higher, functional status was also lower. The significant factors predicting symptom experience were depression, anxiety, activity status, and social support, which explained 57.8% of the variance. Conclusion: These results suggest that psychological factors such as anxiety and depression had a negative influence on the symptom experience of lung cancer patients. Therefore, providing emotional support based on the patients' needs prior to providing symptom management could be a useful strategy for improving symptom experience and functional status.

• Journal of Environmental Science International
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• v.19 no.5
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• pp.565-575
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• 2010

This study is mainly focused on micellar effect of cetylpyridinium chloride(CPyCl) solution including alkylbenzimidazole(R-BI) on dephosphorylation of diphenyl-4-nitrophenylphosphinate(DPNPIN) in carbonate buffer(pH 10.7). The reactions of DPNPIN with R-BI$^{\ominus}$ are strongly catalyzed by the micelles of CPyCl. Dephosphorylation of DPNPIN is accelerated by BI$^{\ominus}$ ion in $10^{-2}M$ carbonate buffer(pH 10.7) of $4{\times}10^{-3}M$ CPyCl solution up to 100 times as compared with the reaction in carbonate buffer by no BI solution of $4{\times}10^{-3}M$ CPyCl. The value of pseudo first order rate constant($k^m_{BI}$) of the reaction in CPyCl solution reached a maximum rate constant increasing micelle concentration. Such rate maxima are typical of micellar catalyzed bimolecular reactions. The reaction mediated by R-BI$^{\ominus}$ in micellar solutions are obviously slower than those by BI$^{\ominus}$, and the reaction rate were decreased with increase of lengths of alkyl groups. It seems due to steric effect of alkyl groups of R-BI$^{\ominus}$ in Stern layer of micellar solution. The surfactant reagent, cetylpyridinium chloride(CPyCl), strongly catalyzes the reaction of diphenyl-4-nitrophenylphosphinate(DPNPIN) with alkylbenzimidazole (R-BI) and its anion(R-BI$^{\ominus}$) in carbonate buffer(pH 10.7). For example, $4{\times}10^{-3}M$ CPyCl in $1{\times}10^{-4}M$ BI solution increase the rate constant ($k_{\Psi}=1.0{\times}10^{-2}sec^{-1}$) of the dephosphorylation by a factor ca.14, when compared with reaction ($k_{\Psi}=7.3{\times}10^{-4}sec^{-1}$) in $1{\times}10^{-4}M$ BI solution(without CPyCl). And no CPyCl solution, in $1{\times}10^{-4}M$ BI solution increase the rate constant ($k_{\Psi}=7.3{\times}10^{-4}sec^{-1}$) of the dephosphorylation by a factor ca.36, when compared with reaction ($k_{\Psi}=2.0{\times}10^{-5}sec^{-1}$) in water solution(without BI). This predicts that the reactivities of R-BI$^{\ominus}$ in the micellar pseudophase are much smaller than that of BI$^{\ominus}$. Due to the hydrophobicity and steric effect of alkyl group substituents, these groups would penetrate into the core of the micelle for stabilization by van der Waals interaction with long alkyl groups of CPyCl.

• Journal of Navigation and Port Research
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• v.33 no.1
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• pp.9-19
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• 2009

This paper describes empirical approach methodology for the quantitative risk assessment of maritime transportation accident (MTA) of a merchant ship. The principal aim of this project is to estimate the risk of MTA that could degrade the ship safety by analyzing the underlying factors contributing to MTA based on the IMO's Formal Safety Assessment techniques and, by assessing the probabilistic risk level of MTA based on the quantitative risk assessment methodology. The probabilistic risk level of MTA to Risk Index (RI) composed with Probability Index (PI) and Severity Index (SI) can be estimated from proposed Maritime Transportation Accident Model (MTAM) based on Bayesian Network with Bayesian theorem Then the applicability of the proposed MTAM can be evaluated using the scenario group with 355 core damaged accident history. As evaluation results, the correction rate of estimated PI, $r_{Acc}$ is shown as 82.8%, the over ranged rate of PI variable sensitivity with $S_p{\gg}1.0$ and $S_p{\ll}1.0$ is shown within 10%, the averaged error of estimated SI, $\bar{d_{SI}}$ is shown as 0.0195 and, the correction rate of estimated RI, $r_{Acc}$(%), is shown as 91.8%. These results clearly shown that the proposed accident model and methodology can be use in the practical maritime transportation field.

• Korean Journal of Oriental Medicine
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• v.17 no.2
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• pp.1-15
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• 2011

In the latter half of the Joseon(朝鮮) Dynasty, the medical world was encountering a great change. It is said that a large stream between the first half and the latter half of the Joseon Dynasty was a qualitative transition from official relationships to private relationships, that is, from adjustments by governmental power to contractual relationships between individuals. Doctors who can be said to be the core of the medical world became to be left in severer competition. The fact that the number of people engaged in medical practice increased to the extent that doctors had to compete with each other implies that not only demand for medical care was increasing but also that medical care was becoming social service that must be shared by all people in the Joseon Dynasty rather than by small numbers of men of power. Anyway, it seems like that, in the competition that was becoming fiercer, they tried to establish their authority in diverse methods unlike before. As an authority to determine the social positions of doctors in the latter half of the Joseon Dynasty, the government was still occupying an important position, but doctors tried to show off their medical techniques utilizing excellent teachers or books. Meanwhile, they were making efforts to improve treating skills and thereby they were contributing to the development of medical techniques although they were sometimes criticised because of radical treatment or fierce drugs. In this process, it seems like that some doctors made efforts to establish the social meaning of medicine and their identity. In the short dialogue with Hong Yangho(洪良浩), Cho Gwangil(趙光一) was presenting the image of doctors as active and subjective beings. Pointing out the fact that in the society where feudal position systems were still impregnable, even the Confucian scholars who could be considered as a leading group could not but be passive in front of the sovereign power, he emphasized the fact that doctors could practice treatment as they liked. In that he re-discovered the meaning of treating people's diseases as a professional intellectual and that he was forming a subjective sense that medical techniques are active self expression, it can be carefully said that Cho Gwangil was obtaining his identity as a doctor. In the society in the Joseon Dynasty where the position systems were still valid and the value system under Neo-confucianism(性理學) supporting the system was impregnable, this change can be thought to be small yet quite meaningful.

• Restorative Dentistry and Endodontics
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• v.34 no.5
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• pp.397-405
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• 2009

This study compared the effect of an activator, intermediate bonding resin and low-viscosity flowable resin on the microtensile bond strength of a self-curing composite resin used with two-step total etching adhesives. Twenty extracted permanent molars were used. The teeth were assigned randomly to nine groups (n=10) according to the adhesive system and application of additional methods (activator, intermediate adhesive, flowable resin). The bonding agents and additional applications of each group were applied to the dentin surfaces. Self-curing composite resin buildups were made for each tooth to form a core, 5mm in height. The restored teeth were then stored in distilled water at room temperature for 24h before sectioning. The microtensile bond strength of all specimens was examined. The data was analyzed statistically by one-way ANOVA and a Scheffe's test. The application of an intermediate bonding resin (Optibond FL adhesive) and low-viscosity flowable resin (Tetric N-flow) produced higher bond strength than that with the activator in all groups. Regardless of the method selected, Optibond solo plus produced the lowest ${\mu}TBS$ to dentin. The failure modes of the tested dentin bonding agents were mostly adhesive failure but there were some cases showed cohesive failure in the resin.