• The Korean Journal of Mycology
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• v.29 no.1
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• pp.34-40
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• 2001

RAPD test and the observation of morphological, cultural characteristics of fourteen selected entomogenous fungi were conducted to investigate the analysis of their internal relationships. Paecilomyces tenuipes showed snow flower form attached to numerous white conidiophores, produced globular and semi-egg types on the club types of phialides. Cordyceps militaris formed globosely conidiophores, dark yellow fruiting body on pupa. The phialide as on Acremonium-type in global conidiophores. Beauveria bassiana covered with conidia was not formed fruiting body and adhered conidia on conidiophore of zigzag type. The PDA and SDAY medium were confirmed as an optimum growth of them. P. tenuipes showed to velvet and plane types in several media whereas C. militaris was belong to centrally raised and floccose in the morphological type. In contrast, B. bassiana covered with conidia on velvet shape. The size of amplified products were analyzed by RAPD using URP primer and were from 100 bp to 2.0 kb with $10{\sim}14$ geuomic DNA. Total similarities of two groups were by dendrogram of UPGMA analysis. The homology of P. tenuipes groups was 94.8 to 100%. It also showed 70.1 to 96.6% in C. militaris group and B. bassiana was higher similarity than any other. The internal change of C. militaris, produced telemorph fruiting body, was higher seperated in species than P. tenuipes and B. bassiana in the RAPD.

• Journal of Life Science
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• v.23 no.7
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• pp.904-912
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• 2013

This study aimed to evaluate the protective effect of cordycepin-increased Cordyceps militaris strain on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity and oxidative stress in rats. Male Sprague-Dawley rats were randomly divided into five groups (n=6) based on six dietary categories: normal (N), $CCl_4$ control (C), $CCl_4$ plus Paecilomyces japonica (CPJ) (3%, w/w), $CCl_4$ plus C. militaris (CCM) (3%, w/w), and $CCl_4$ plus cordycepin-increased C. militaris ($CCM{\alpha}$) (3%, w/w). The activities of the liver marker enzymes ALT, AST, and LDH and the levels of lipid peroxidation were increased in the $CCl_4$-treated groups, but these parameters were significantly decreased in the $CCM{\alpha}$ group. The TBARS content in the liver homogenate, microsome, and mitochondrial fractions of the C group was significantly elevated compared with the N group. However, in the $CCl_4$-treated groups, $CCM{\alpha}$ group was significantly lowered in the TBARS levels of hepatic homogenate and microsomal fractions. The C group showed a significant decrease in the levels of plasma and hepatic glutathione, whereas they were significantly increased in the $CCM{\alpha}$ group. Accordingly, cordycepin-increased C. militaris may be an ideal animal model for studying hepatoprotective effects.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.169-170
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• 2002

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.65.1-65.1
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• 2007

See Full Text

• Proceedings of the Korean Society of Life Science Conference
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• 2002.03a
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• pp.105-105
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• 2002

• Proceedings of the Zoological Society Korea Conference
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• 2001.04a
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• pp.79.2-79
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• 2001

No Abstract, See Full Text

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.190.2-190
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• 1999

No Abstract, See Full Text

• The Journal of Internal Korean Medicine
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• v.21 no.3
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• pp.389-398
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• 2000

The fallowing are the results of the experimental studies of Cordyceps militaris (CM) on the penile nitric oxide synthase (NOS) activity and the level of blood testosterone in hydrocortisone acetate-treated rats. CM was tested for the effects on activity of xanthine oxidase and lipid peroxidation in penis of hydrocortisone acetate-treated rats. In vitro, CM didn't effect the levels of lipid peroxide and the activity of NOS. In the penis of hydrocortisone acetate-treated rats, lipid peroxide, the activities and ratio of type conversion of xanthine oxidase were increased but activity of NOS and content of nitrite were decreased. In vivo, after administration of CM to hydrocortisone acetate-treated rats, levels of lipid peroxide in penis was decreased. Also, the activities and ratio of type conversion of xanthine oxidase were decreased, too. The body weight and concentration of testosterone in the blood were increased. The effects of Cordyceps militalis Broth did better than the effects of Cordyceps militalls Mycelia, These results suggest that CM decrease the activities of free radical generating enzymes such as xanthine oxidase which form lipid peroxide and increase the penile NOS activity and the level of blood testosterone in hydrocortisone acetate-treated rats. Conclusively, CM is capable of improving of sexual ability in hydrocortisone acetate-treated rats.

• Journal of Mushroom
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• v.7 no.4
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• pp.182-186
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• 2009

Single ascospores was isolated in Yesan-gun, Chungnam in 2006 with Cordyceps militaris. This study was carried out to select the superior isolates which produce more fruiting body and contain more cordycepin contents. Five single ascospore isolates were cultured on brown rice pupae medium and they formed fruiting bodies. To select better isolates, each isolates was hybridized and then the characteristics and cordycepin contents were investigated. After biological efficiency and codycepin contents were examined, finally C0601S05 was selected. Top-cross test of isolate C0601S05 was carried with 29 single ascospores. From this test, isolate C0601S05${\times}$C0601S33 and isolate C0601S05${\times}$C0601S43 containing highest cordycepin contents were selected. Codycepin contents of C0601S05 was 246mg% and those of C0601S05${\times}$C0601S33 and C0601S05${\times}$C0601S43 were 325mg% and 280mg%, respectively. C0601S05${\times}$C0601S33 was registered as Yedang 3 at the Korea seed & variety service.

• The Korean Journal of Mycology
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• v.30 no.1
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• pp.6-10
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• 2002

The isolates of Cordyceps militaris preserved in EFCC, Kangwon National University were investigated to form the fruiting bodies under artificial conditions. The fruiting bodies were observed to be better in the 1l polyethylene bottle containing $60{\sim}80\;gm$ of brown rice and $100{\sim}110\;ml$ of water. Addition of $10{\sim}20\;gm$ of pupae per bottle showed higher fruiting. Similarly, addition of sucrose, peptone or hemoglobin also had favorable effect on fruiting. $25^{\circ}C\;and\;20^{\circ}C$ were favorable for mycelial growth and fruiting respectively. Light intensity of 500 lux and 12 h of light/dark period produced highest amount of fruit bodies.