The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Investigation on Artificial Fruiting of Cordyceps militaris

번데기동충하초의 자실체 형성에 관한 연구

  • Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University) ;
  • Choi, Young-Sang (Department of Environmental Biology, Kangwon National University) ;
  • Shrestha, Bhushan (Department of Environmental Biology, Kangwon National University) ;
  • Park, Young-Joon (Department of Environmental Biology, Kangwon National University)
  • Published : 2002.04.30
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Abstract

The isolates of Cordyceps militaris preserved in EFCC, Kangwon National University were investigated to form the fruiting bodies under artificial conditions. The fruiting bodies were observed to be better in the 1l polyethylene bottle containing $60{\sim}80\;gm$ of brown rice and $100{\sim}110\;ml$ of water. Addition of $10{\sim}20\;gm$ of pupae per bottle showed higher fruiting. Similarly, addition of sucrose, peptone or hemoglobin also had favorable effect on fruiting. $25^{\circ}C\;and\;20^{\circ}C$ were favorable for mycelial growth and fruiting respectively. Light intensity of 500 lux and 12 h of light/dark period produced highest amount of fruit bodies.

배양병의 선발시험에서는 1100 cc pp병에서 자실체 형성이 우수하였다. 경제성을 고려하여 1000 cc병을 이용한 자실체형성에서 현미의 입병량은 $60{\sim}80\;g$이 적당하였다. 자실체 형성을 위한 배양온도는 $24^{\circ}C$에서 균사 배양한 후 $20^{\circ}C$에서 발아를 유도하였을 때 자실체 형성이 우수하였다. 현미배지의 sucrose첨가량은 10%를 첨가하고 번데기첨가량은 병당 10 g을 첨가하였을 때 자실체 형성이 양호하였으며 peptone과 hemoglobin, 건조누에 등은 자실체의 수확량을 증가시키지 못하였다. 현미배지의 공극률에서는 $0.59{\sim}0.63%$의 공극률에서 자실체 형성이 양호하였다. 광조사의 시간은 500 lx, 12시간 정도가 적당하였다.

Keywords

References

  1. 성재모. 1996. 한국의 동충하초. 교학사.
  2. 李賢卿. 1996. 韓國産 冬蟲夏草의 分布와 分類 및 培養的特性에 관한 硏究. 江原大學校 碩士學位 論文.
  3. Basith, M. and Madelin, M. F. 1968. Studies on the production of perithecial stromata by Cordyceps militaris in artificial culture. Can. J. Bot. 46: 473-480. https://doi.org/10.1139/b68-071
  4. Choi, I. Y., Choi, J. S., Lee, W. H., Yu, Y. J., Joung, G. T., Ju, L. O. and Choi, Y. K. 1999. The condition of production of artificial fruiting body of Cordyceps militaris. Kor. J. Mycol. 27(4): 243-248.
  5. Glare, T. R. and Milner. R. J. 1991. Ecology of entomopathogenic fungi. In: Handbook of Applied Mycology. Vol. 2. Ed. D. K. Arora, L. Ajello, and K.G. Mukergi. Marcel Dekker, Inc.
  6. Humber, R. A. 2000. Fungal pathogens and parasites of insects. In: Applied Microbial Systematics. Ed. F. G. Priest and M. Goodfellow. Kluwer academic Publishers.
  7. Kobayasi, Y. 1941. The genus Cordyceps and its allies. Sei. Rept. Tokyo Buntika Daigaku, sect. B.5: 53-260.
  8. Kobayasi, Y. 1982. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans. Mycol. Soc. Japan 23: 329-364.
  9. Liang, Z. Q. 1990. Anarnorph of Cordyceps militaris and artificial culture of its fruitbody. Southwest China J. Agri. Sci. 3(2):1-6
  10. Mains, E. B. 1958. North American entomogenous species of Cordyceps. Mycologia 50: 169-222. https://doi.org/10.2307/3756193
  11. Samson, R. A. 1974. Paecilomyces and some allied Hyphomycetes. Studies in Mycology 6: 31-36.
  12. Pen, X. 1995. The cultivation of Cordyceps militaris fruitbody on artificial media and the determination of SOD activity. Acta Edulis Fungi. 2(3): 25-28.
  13. Seaver, F. J. 1911. The Hypocreales of North America- IV. Mycologia 3: 207-230. https://doi.org/10.2307/3753505
  14. Shanor, L. 1936. The production of mature perithecia of Cordycpes militaris (L.) Link in laboratory culture. J. Elisha Mitchell Sci. Soc. 52: 99-105.
  15. Sung, J. M., Choi, Y. S., Lee. H. K, Kim, S. H., Kim, Y O. and Sung, G. H. 1999. Production of fruiting body using cultures of entomopathogenic fungal species. Kor. J. Mycol. 27(1): 15-19.
  16. Sung, J. M., Kim, C., H. Yang, K. J., Lee, H. K. and Kim, Y. S. 1993. Studies on distribution and utilization of Cordyceps militaris and C. nutans. Kor. J. Mycol. 21(2): 94-105.
  17. Sung, J. M., Lee, H. K., Choi, Y. S. Kim, Y. O. Kim, S. H. and Sung, G. H. 1997. Distribution and taxonomy of entomopathogenic fungal species from Korea Kor. J. Mycol. 25(4): 231-252.
  18. Zeng. M and Kinjo, N. 1998. Notes on the alpine Cordyceps of China and nearby nations. Mycotaxon 46: 215-229.

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