• The Plant Pathology Journal
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• 제15권1호
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• pp.14-20
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• 1999

Twenty isolates of Helminthosporium species were obtained from various grass plants and tested for controlling efficacy on the development of plant diseases. An isolate of Helminthosporium sp. TP-4 was chosen and six antibiotic substances were purified from cultures of the fungus by repeated silica gel column chromatography and preparative thin-layer chromatography. They were identified as ophiobolin a, 6-epiophiobolin A, 3-anhydroophiobolin A, 3-anhydro-6-epiophiobolin A, iphiobolin B, and iphiobolin I mainly by mass spectrometry and nuclear magnetic resonance spectrometry. Ophiobolins inhibited the growth of a grampositive bacterium Streptomyces griseus, but were not active against gram-negative bacteria. They also showed an antifungal activity. In in vivo tests, iphiobolin B exhibited potent controlling activities against rice blast, tomato late blight, and wheat leaf rust with control values more than 90% and 70% at concentration of $500\mu\textrm{m}$/ml and 100 ${\mu}{\textrm}{m}$/ml. Ophiobolin A and 6-epiophiobolin A controlled the development of wheat leaf rust more than 80% at concentrations of 100 /ml and $500\mu\textrm{m}$/ml respectively. 3-Anhydro-6-epiophiobolin A was not active against any plant disease. On the other hand, the A-series ophiobolins other than 3-anhydroophiobolin A showed stronger phytotoxic activity in a leaf-wounding assay using 8 plant species than those of 3-anhydroophiobolin A, ophiobolin B, and ophiobolin I. The results indicate that there is little correlation between antifungal activity and phytotoxicity of ophiobolins.

• 터널과지하공간
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• 제20권1호
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• pp.65-72
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• 2010

암반발파에서 평활한 파단면을 확보하고 굴착손상영역을 제어하기 위하여 노치장약공을 이용한 균열제어공법이 제안되었다. 본 연구에서는 노치형상과 장약조건이 균열발생 및 성장에 미치는 영향을 살펴보기 위하여 날개형 노치장약공을 갖는 발파모델을 구축하고 동적 파괴과정 해석법을 이용한 암반 파괴과정 해석을 수행하였다. 그 결과, 노치 길이가 증가함에 균열의 성장 길이가 증가하며 파단면의 거칠기가 감소하고 장약공 상하부에 손상균열의 발생이 억제되는 경향을 보였다. 해석결과로부터 노치 길이 및 개구 폭에 따른 응력집중계수의 변화 및 균열발생 양상을 비교 분석하여 균열제어에 미치는 영향인자에 대하여 고찰하였다.

• Computers and Concrete
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• 제12권6호
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• pp.785-802
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• 2013

Ready-mixed soil material, known as a kind of controlled low-strength material, is a new way of soil cement combination. It can be used as backfill materials. In this paper, artificial neural network and nonlinear regression approach were applied to predict the compressive strength of ready-mixed soil material containing Portland cement, slag, sand, and soil in mixture. The data used for analyzing were obtained from our testing program. In the experiment, we carried out a mix design with three proportions of sand to soil (e.g., 6:4, 5:5, and 4:6). In addition, blast furnace slag partially replaced cement to improve workability, whereas the water-to-binder ratio was fixed. Testing was conducted on samples to estimate its engineering properties as per ASTM such as flowability, strength, and pulse velocity. Based on testing data, the empirical pulse velocity-strength correlation was established by regression method. Next, three topologies of neural network were developed to predict the strength, namely ANN-I, ANN-II, and ANN-III. The first two models are back-propagation feed-forward networks, and the other one is radial basis neural network. The results show that the compressive strength of ready-mixed soil material can be well-predicted from neural networks. Among all currently proposed neural network models, the ANN-I gives the best prediction because it is closest to the actual strength. Moreover, considering combination of pulse velocity and other factors, viz. curing time, and material contents in mixture, the proposed neural networks offer better evaluation than interpolated from pulse velocity only.

• 한국미생물·생명공학회지
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• 제30권4호
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• pp.339-345
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• 2002

가지에서 분리한 F. equiseti FO-68균주와 벗풀에서 분리한 Fusarium sp. FO-510균주로부터 항균물질을 분리한 후 이들의 식물병원곰팡이에 대한 항균활성을 in vitro및 in vivo에서 조사하였다. FO-68균주의 쌀배양체로부터 하나의 항생물질을 순화하였는데 , 이 물질은 equisetin이라는 물질로 동정되었다. 그리고 FO-510균주의 쌀배양체로부터는 두 개의 항균활성 물질을 분리하였는데, 이들은 zearalenone과 8'-hydroxyzearalenone으로 동정되었다. Equisetin과 zearalenone은 in vitro에서 실험한 식물병원곰팡이 대부분에 대해서 높은 항균활성을 보였지만, 8'-hydroxyzearalenone은 거의 항균활성이 없었다. In vivo assay에서 equisetin은 토마토ㆍ잿빛곰팡이병과 토마토ㆍ역병에 방제효과가 컸으며, zearalenone은 벼ㆍ도열병, 벼ㆍ잎집무의마름병, 토마토ㆍ잿빛곰팡이병 및 토마토ㆍ역병에 대하여 효과를 나타내었다. 하지만 8'-hydroxyzearalenone는 토마토ㆍ잿빛곰팡이병을 제외한 나머지 식물병의 발생은 억제하지 못했다. Equisetin, zearalenone 및 8'-hydroxyzearalenone의 항진균활성은 본 논문에서 처음으로 보고하는 바이다.

• Journal of Periodontal and Implant Science
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• 제46권5호
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• pp.337-349
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• 2016

Purpose: The aim of this study was to compare and analyze the peri-implant tissue conditions and prospective clinical outcomes associated with 2 types of hydroxyapatite (HA)-coated implants: (1) fully HA-coated implants and (2) partially HA-coated implants with resorbable blast medium on the coronal portion of the threads. Methods: Forty-four partially edentulous patients were randomly assigned to undergo the placement of 62 HA-coated implants, and were classified as the control group (partially HA-coated, n=30) and the test group (fully HA-coated, n=32). All patients had chronic periodontitis with moderate crestal bone loss around the edentulous area. The stability and clinical outcomes of the implants were evaluated using the primary and secondary implant stability quotient (ISQ), as well as radiographic, mobility, and peri-implant soft tissue assessments around the implants. The Wilcoxon signed-rank test and the Mann-Whitney test were used to evaluate differences between and within the 2 groups, with P values <0.05 considered to indicate statistical significance. Results: The fully HA-coated implants displayed good retention of crestal bone, and insignificant differences were found in annual marginal bone loss between the 2 types of HA-coated implants (P>0.05). No significant differences were found in the survival rate (group I, 100%; group II, 100%) or the success rate (group I, 93.3%; group II, 93.8%). The fully HA-coated implants also did not significantly increase the risk of peri-implantitis (P>0.05). Conclusions: The fully HA-coated implants did not lead to an increased risk of peri-implantitis and showed good retention of the crestal bone, as well as good survival and success rates. Our study suggests that fully HA-coated implants could become a reliable treatment alternative for edentulous posterior sites and are capable of providing good retention of the crestal bone.

• Structural Engineering and Mechanics
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• 제60권5호
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• pp.809-828
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• 2016

In deep open pit mines, slope stability is very important. Particularly, increasing the depths increase the risks in mines having weak rock mass. Blasting operations in this type of open pits may have a negative impact on slope stability. Several or combination of methods can be used in order to enable better analysis in this type of deep open-pit mines. Numerical modeling is one of these options. Many complex problems can be integrated into numerical methods at the same time and analysis, solutions can be performed on a single model. Rock failure criterions and rock models are used in numerical modeling. Hoek-Brown and Mohr-Coulomb terms are the two most commonly used rock failure conditions. In this study, mine planning and discontinuity conditions of a lignite mine facing two big landslides previously, has been investigated. Moreover, the presence of some damage before starting the study was identified in surrounding structures. The primary research of this study is on slope study. In slope stability analysis, numerical modeling methods with Hoek-Brown and Mohr-Coulomb failure criterions were used separately. Preparing the input data to the numerical model, the outcomes of patented-blast vibration minimization method, developed by co-author was used. The analysis showed that, the model prepared by applying Hoek-Brown failure criterion, failed in the stage of 10. However, the model prepared by using Mohr-Coulomb failure criterion did not fail even in the stage 17. Examining the full research field, there has been ongoing production in this mine without any failure and damage to surface structures.

• Journal of Microbiology and Biotechnology
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• 제15권1호
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• pp.112-117
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• 2005

Abstract Griseofulvin has been used as an antifungal antibiotic for the treatment of mycotic diseases of humans and veterinary animals. The purpose of this work was to identify a griseofulvin-producing endophytic fungus from Abies holophylla and evaluate its in vivo antifungal activity against plant pathogenic fungi. Based on nuclear ribosomal ITS1-5.8SITS2 sequence analysis, the fungus was identified and labeled as Xylaria sp. F0010. Two antifungal substances were purified from liquid cultures of Xylaria sp. F0010, and their chemical identities were determined to be griseofulvin and dechlorogriseofulvin through mass and NMR spectral analyses. Compared to dechlorogriseofulvin, griseofulvin showed high in vivo and in vitro antifungal activity, and effectively controlled the development of rice blast (Magnaporthe grisea), rice sheath blight (Corticium sasaki), wheat leaf rust (Puccinia recondita), and barley powdery mildew (Blumeria graminis f. sp. hordei), at doses of 50 to 150 ${\mu}$g/ml, depending on the disease. This is the first report on the production of griseofulvin and dechlorogriseofulvin by Xylaria species.

• 방사성폐기물학회지
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• 제2권4호
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• pp.253-262
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• 2004

Construction of tunnels in a deep crystalline host rock for a potential High-Level Radioactive Waste(HLW) repository inevitably generates an excavation disturbed zone (EDZ). There have been a series of debates on whether a permeability in an EDZ increases or not and what would be the maximum depth of an EDZ. Recent studies show mixed opinions on permeability. However, there has been an international consensus on the thickness of an EDZ; 30 cm for TBM and 1 meter for controlled blast. One of the impacts of an EDZ is on determining the distance between adjacent deposition holes. The void gap by the excavation hinders relaxation of temperature profiles so that the current Korean reference designing distance between holes should be stretched out more to keep the maximum temperature in a buffer region below 100 degrees Celsius. The other impact of an EDZ is on the long-term post closure radiological safety. To estimate the impact, the reference scenario, the well scenario, is chosen. Released nuclides diffuse through a bentonite buffer region experiencing strong sorption and reach a fracture surrounded by a porous medium. Inside a fractured porous region, radionuclides migrate by advection and dispersion with matrix diffusion into a porous medium. Finally, they reach a well assumed to be a source of potable water for local residents. The annual individual dose is assessed on this well scenario to find out the significance of an EDZ. A profound sensitivity study was performed, but all results show that the impact is negligible. Even though the role of an EDZ turns out to be limited on overall safety assessment, still it is worthwhile to study the chemical role of an EDZ, such as a potential source for natural colloids, potential sealing of an open fracture by fine clay particles generated by the process of an EDZ, and alteration of a sorption mechanism by an EDZ in the future.

• 한국건축시공학회지
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• 제15권4호
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• pp.383-389
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• 2015

ALC 블록(0.6품) 성능 수준의 습식형 경량 기포콘크리트 배합설계 제시를 위하여 7배합의 섬유가 혼입된 기포콘크리트 배합실험을 수행하였다. 수화열에 의한 온도균열제어를 위해 결합재에 고로슬래그를 30% 치환하였으며, 기포콘크리트의 균열저항성을 높이기 위하여 PVA 및 PA섬유를 혼입하였다. 고로슬래그를 30% 치환한 결합재는 시멘트 100% 대비 최대 열발생률 및 콘크리트 내부 최고 온도를 각각 28% 및 9% 저감시킬 수 있었다. 실험결과 섬유가 혼입된 경량기포 콘크리트의 압축강도, 공극분포, 휨 강도를 고려하면 PVA 및 PA 섬유 $0.6kg/m^3$ 혼입이 추천될 수 있었다.

• 한국응용곤충학회지
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• 제12권1호
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• pp.33-39
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• 1973

시비량의 증시에 따라 수량은 증가하지만 어느 일정수준 이상 과용하던 병충해발생을 유발하여 수량의 감소를 초래하므로 시비량을 현행수준 보다 올리는 반면 병충해 방제를 강화하여 병충해피해를 줄이고 최대한의 증수를 가져 올 수 있는 적정시비수준과 병충해 방제효과를 구명하고자 본 시침을 실시하여 다음과 같은 결과를 얻었다. (1) 잎도열병, 이삭도열병 발병율은 다 같이 시비량과 $1\%$수준에서 높은 정(+)의 상관을 나타내었고 인산과 가리를 과용하면 삼요소 표준구보다 발병이 훨씬 많았다. (2) 병충해방제를 실시하지 않을 때는 시비량을 질소기준 8-8.5kg/10a 시용하였을 때 347-378kg/10a의 최고현미수량을 얻었는데 비하여 병충해방제를 실시하면 질소기준 18.5-19kg/10a수준에서 453-462kg/10a의 최고수량을 나타내었고 병충해방제 실시여부에 따른 수량의 차이는 평균 100kg/10a을 상회하였다. (3) 질소비료만 증시한 구와 삼요소비료를 동시에 증시한 구 사이의 수량에 미치는 영 향은 유의 차가 없었다. (4) 현미수량지수로 환산한 병충해탕제효과는 최고 $65\%$에서 최저 $30\%$까지 나타났으며 평균방제효과는 $42-45\%$였다.