• Title/Summary/Keyword: controlled blast

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Characterization of Rice Mutants with Enhanced Susceptibility to Rice Blast

  • Kim, Hye-Kyung;Lee, Sang-Kyu;Cho, Jung-Il;Lee, Sichul;An, Gynheung;Jwa, Nam-Soo;Kim, Byung-Ryun;Cho, Young-Chan;Han, Seong-Sook;Bhoo, Seong-Hee;Lee, Youn-Hyung;Hong, Yeon-Kyu;Yi, Gihwan;Park, Dae-Sup;Hahn, Tae-Ryong;Jeon, Jong-Seong
    • Molecules and Cells
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    • v.20 no.3
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    • pp.385-391
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    • 2005
  • As a first step towards identifying genes involving in the signal transduction pathways mediating rice blast resistance, we isolated 3 mutants lines that showed enhanced susceptibility to rice blast KJ105 (91-033) from a T-DNA insertion library of the japonica rice cultivar, Hwayeong. Since none of the susceptible phenotypes co-segregated with the T-DNA insertion we adapted a map-based cloning strategy to isolate the gene(s) responsible for the enhanced susceptibility of the Hwayeong mutants. A genetic mapping population was produced by crossing the resistant wild type Hwayeong with the susceptible cultivar, Nagdong. Chi-square analysis of the $F_2$ segregating population indicated that resistance in Hwayeong was controlled by a single major gene that we tentatively named Pi-hy. Randomly selected susceptible plants in the $F_2$ population were used to build an initial map of Pi-hy. The SSLP marker RM2265 on chromosome 2 was closely linked to resistance. High resolution mapping using 105 $F_2$ plants revealed that the resistance gene was tightly linked, or identical, to Pib, a resistance gene with a nucleotide binding sequence and leucine-rich repeats (NB-LRR) previously isolated. Sequence analysis of the Pib locus amplified from three susceptible mutants revealed lesions within this gene, demonstrating that the Pi-hy gene is Pib. The Pib mutations in 1D-22-10-13, 1D-54-16-8, and 1C-143-16-1 were, respectively, a missense mutation in the conserved NB domain 3, a nonsense mutation in the 5th LRR, and a nonsense mutation in the C terminus following the LRRs that causes a small deletion of the C terminus. These findings provide evidence that NB domain 3 and the C terminus are required for full activity of the plant R gene. They also suggest that alterations of the resistance gene can cause major differences in pathogen specificity by affecting interactions with an avirulence factor.

A Study on the Effect of Artificial Cutting Slot on the Fragmentation and Vibration Propagation in the Full-scaled Concrete Block Blasting (콘크리트 블록 발파 실험을 통한 인공 슬롯 자유면이 진동전파 및 파쇄효과에 미치는 영향에 관한 연구)

  • Oh, Se-Wook;Min, Gyeong-Jo;Park, Se-Woong;Park, Hoon;Noh, You-Song;Suk, Chul-Gi;Cho, Sang-Ho
    • Tunnel and Underground Space
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    • v.28 no.6
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    • pp.692-705
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    • 2018
  • Ground vibration is one of the remarkable issues in tunnel blasting. In recent studies, to improve the fragmentation with reduction of ground vibration in tunnel blasting, a vibration-controlled blasting method with artificial cutting slot near the center-cut holes has been suggested. This study examines the effect of the different arrangement of artificial cut-slot on the vibration reduction and fragmentation by performing the full-scaled concrete block blast experiments and the numerical simulations with 3D-DFPA. The results show that the existence of artificial slot contributes to the improvement of vibration reduction, blast fragmentation and the efficiency of the cutting slot blast. It can be explained that the artificial slot play a free surface role and should decrease the burden between the cut holes. Crater volumes of the blasted concrete blocks were measured by 3-dimensional digital image analysis and compared with the ideal standard crater volume which can be calculated by theoretical standard blast design method. As a result, the ratio of burden and hole diameter which should achieve the standard crater in the cut-hole blasting were suggested.

Mapping and Race Specific Reaction of the Resistance Gene Pi45(t) in Rice (벼 도열병 저항성 유전자 Pi45(t)의 균계 특이적 반응과 고밀도지도 작성)

  • Kim, Dong-Min;Ju, Hong-Guang;Yang, Paul;Han, Seong-Sook;Roh, Jae-Hwan;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.43 no.1
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    • pp.42-49
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    • 2011
  • QTL analysis for blast resistance was carried out using 140 $BC_3F_3$ lines derived from a cross between Ilpum as a recurrent parent and Moroberekan as a donor parent. 140 $BC_3F_3$ lines with the parents were inoculated with nine blast isolates. To identify QTLs for resistance to nine blast isolates, 134 SSR markers showing polymorphisms between the parents were genotyped for the 140 $BC_3F_3$ lines. A total of 17 resistance QTLs to nine isolates were detected on chromosomes 2, 3, 4, 6, 7, 9 and 10. The phenotypic variance explained by each QTL ranged from 8.2% to 26.4%. The Moroberekan alleles contributed the positive effect at these 17 QTL loci. In a previous study, the QTL, Pi45(t) for durable resistance to blast was identified using a sequential planting method. To know the relationship between Pi45(t) and the isolate-specific resistance gene, an $F_2$ population was developed from a cross between Ilpum and an introgression line harboring Pi45(t). $F_3$ lines segregating for the Pi45(t) were inoculated to three isolates. $F_3$ lines from the $F_2$ plants with the Moroberekan segment at the target region showed resistance to two isolates. This result seems to indicate that the Pi45(t) and the isolate-specific resistance gene are tightly linked or the resistance is controlled by the same gene(s). The markers linked to genes controlling blast resistance would be useful in developing blast resistance lines in the breeding program.

Pallet speed control in a sintering plant using neural networks (신경회로망을 이용한 소결기 팰릿 속도 제어)

  • Jang, Min;Cho, Sung-Jun
    • Proceedings of the Korea Database Society Conference
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    • 1999.06a
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    • pp.261-270
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    • 1999
  • Sintering transforms powdered ore into lumped ore so that the latter can be used in a blast furnace. The powdered ore combined with coke and other materials is loaded into a container and moved along by a pallet while the ignited coke bums. The speed by which the pallet moves determines how much sintering takes place. Since the process is complicated and lacks an accurate mathematical model, human operators manually control the speed by monitoring various factors in the plant. In this paper, we propose a neural network-based pallet speed controller which copies human operator knowledge. Actual process data were collected from a sintering plant fer eight months and preprocessed to remove noisy and inconsistent data. A multilayer perceptron was trained using a back-propagation learning algorithm. In on-line testing at the sinter plant, the proposed model reliably controlled pallet speed during normal operation without the help of human operators. Moreover, the duality and productivity was as good as with human operators.

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Pallet speed control in a sintering plant using neural networks (신경회로망을 이용한 소결기 팰릿 속도 제어)

  • Jang, Min;Cho, Sung-Jun
    • Proceedings of the Korea Inteligent Information System Society Conference
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    • 1999.03a
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    • pp.261-270
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    • 1999
  • Sintering transforms powdered ore into lumped ore so that the latter can be used in a blast furnace. The powdered or combined with coke and other materials is loaded into a container and moved along by a pallet while the ignited coke burns. The speed by which the pallet moves determines how much sintering takes place. Since the process is complicated and lacks an accurate mathematical model, human operators manually control the speed by monitoring various factors in the plant. In this paper, we propose a neural network-based pallet speed controller which copies human operator knowledge. Actual process data were collected from a sintering plant for eight months and preprocessed to remove noisy and inconsistent data. A multilayer perceptron was trained using a back-propagation learning algorithm. In on-line testing at the sinter plant, the proposed model reliably controlled pallet speed during normal operation without the help of human operators. Moreover, the quality and productivity was as good as with human operators.

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Isolation and Identification of Myxobacteria KR025 and Searching of Their Bioactive Compounds (점액세균 KR025의 분리 동정 및 생리활성물질의 탐색)

  • 김병섭;안종웅;조광연
    • Korean Journal Plant Pathology
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    • v.14 no.4
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    • pp.345-349
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    • 1998
  • Fifty isolates of myxobacteria were isolated from soils from several areas in Korea during 1996-1997 and bioactivity against plant pathogenic fungi of these isolates was examined. A myxobacterial isolate KR025 showed good antifungal activities against Pyricularia oryzae, Cryphonectria parasitica, Colletotrichum lagenarium, and C. gloeosporioides but did not against Rhizoctonia solani, Fusarium oxysporum and Pythium ultimum. The bacterium was identified as Myxococcus fulvus based on morphological and physiological characteristics. Antifungal substances were extracted from culture broth and bacterial cell of Myxococcus fulvus KR025 by ethyl acetate. Antifungal substance of Myxothiazole (100 ${\mu}{\textrm}{m}$/ml) produced by Myxococcus fulvus KR 025 controlled 97.0% rice blast, tomato late blight, wheat leaf rust, and barley powdery mildew and showed 45.0 and 82.6% disease control of rice sheath blight and cucumber gray model, respectively.

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Experimental studies on rheological properties of smart dynamic concrete

  • Bauchkara, Sunil D.;Chore, H.S.
    • Advances in concrete construction
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    • v.5 no.3
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    • pp.183-199
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    • 2017
  • This paper reports an experimental study into the rheological behaviour of Smart Dynamic Concrete (SDC). The investigation is aimed at quantifying the effect of the varying amount of mineral admixtures on the rheology, setting time and compressive strength of SDC containing natural sand and crushed sand. Ordinary Portland cement (OPC) in conjunction with the mineral admixtures was used in different replacement ratio keeping the mix paste volume (35%) and water binder ratio (0.4) constant at controlled laboratory atmospheric temperature ($33^{\circ}C$ to $35^{\circ}C$). The results show that the properties and amount of fine aggregate have a strong influence on the admixture demand for similar initial workability, i.e., flow. The large amounts of fines and lower value of fineness modulus (FM) of natural sand primarily increases the yield stress of the SDC. The mineral admixtures at various replacement ratios strongly contribute to the yield stress and plastic viscosity of SDC due to inter particle friction and cohesion.

Recent Improvements in Integrated Zinc Control and Dust/Sludge Recycling at China Steel

  • Liu, Kuo-chung;Chiang, Shyh-yi
    • Proceedings of the IEEK Conference
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    • 2001.10a
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    • pp.437-442
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    • 2001
  • In an integrated steel mill, zinc (Zn) inputs from raw materials and steel scrap are enriched in the dusts and sludges collected from gas and water cleaning systems. The reuse of these dusts and sludges must be controlled within certain limit to avoid Zn accumulation and related operational problems in blast furnace. An integrated system has been established at China Steel Corporation (CSC) to enhance the internal reuse of Zn-containing dust/sludge while keeping Zn input within control lim it. However. the performance of this system has not been very satisfactory until one and half years ago when a rationalization process was initiated. The essence of this rationalization process, the recent improvements in Zn control and dust and sludge reuse are reported and discussed.

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A study on characteristics of blast vibration waveform by vibration time history analysis (진동이력분석을 응용한 발파 진동파형의 특성에 관한 연구)

  • 김진수;임한욱
    • Tunnel and Underground Space
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    • v.9 no.1
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    • pp.36-47
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    • 1999
  • For cautious controlled blasting, it is necessary to understand characteristics of the blasting vibration. In this study, a series of tests were carried out to investigate the several characteristics of blasting vibration waveform by vibration time history analysis. Separation between impulse vibration and free vibration from blasting vibration, duration time, effects of overlap of free vibration upon the level of vibration and changes of waveform according to increase of charge weight per delay etc. were studied with waveform analysis.

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The Influence of Ground Vibration Caused by Pile Driving on Power Line Tower Foundation (항타 진동이 송전탑 기초에 미치는 영향 연구)

  • Park, Jung-Bong
    • Explosives and Blasting
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    • v.27 no.2
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    • pp.42-47
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    • 2009
  • Ground vibrations caused by pile driving or explosive blasting can affect the stability of power line tower and its foundation. Because the characteristics of ground vibrations generally depend on the distances from the blast, the ground vibrations should be controlled by taking the distance into account. In this study, ground vibration levels were measured at the foundation of a power line tower and on ground surface adjacent to the tower. The relationships between the dominant frequencies of the ground vibrations that were measured at both locations were comparatively investigated.